Retrospective analysis of molecular biology mechanism of ABO blood group typing discrepancy among blood donors in Jinan blood station.

BACKGROUND: To accurately identify ABO blood typing in pre-transfusion testing is very important to ensure blood transfusion safely, which is a major responsibility of blood station. METHODS: Eighty-one blood donors samples with ABO blood group typing discrepancy was collected among 61952 donor samples in our blood station from January 2019 to July 2020. Blood group serological method was used to detect ABO blood group. DNA Sequencing was used to determine the genotype. The antibody screening test detects antibodies other than ABO. RESULTS: In total, 61,952 donor samples were analysed for ABO typing discrepancies. The incidence among blood donors was 0.13% (81/61952). The most common reason of ABO typing discrepancies was due to specific antibody or non-specific agglutination (54.32%, 44/81), mainly anti-M antibody, cold autoantibody, anti-D antibody, anti-N antibody and anti-Lea antibody. The major cause of forward typing discrepancies among blood donors was ABO subgroups (25.93%, 21/81), including 10 cases of A subtype (1 case of A2, 2 cases of A3, 2 cases of Ax, 3 cases of AxB, 1 case of Ael, 1 case of Ahm), 6 cases of B subtype (2 cases of B3, 1 case of Bel, 3 cases of AB3), 2 cases of B subtype (A), 1 case of cisAB, and 2 cases of acquired B. The serum antibody was weakened in 16 cases (19.75%). CONCLUSIONS: The blood types should be correctly identified by combining serology with gene sequencing to ensure the safety of clinical blood transfusion, when the forward and reverse typing discrepancies among the blood donors.

Expression of Wnt-1 and TSLC1 in condyloma acuminatum.

BACKGROUND: Despite its high contagiousness, high recurrence rate and potential for malignant transformation, effective treatments for condyloma acuminatum (CA) have not yet been developed. Accordingly, it is necessary to clarify the mechanisms underlying CA development. AIM: To investigate the expression and significance of the proteins Wnt-1 and TSLC1 in patients with CA and in normal foreskin controls. METHODS: Wnt-1 and TSLC1 were assessed by immunohistochemistry in 45 patients with CA. RESULTS: Positive expression rates of Wnt-1 and TSLC1 were 82.22% (37/45) and 37.78% (17/45), respectively, in CA tissues, and 29.17% (7/24) and 91.67% (22/24), respectively, in normal foreskin controls. Wnt-1 expression intensity in CA was markedly higher (positive to strongly positive) than that in normal controls (negative to weakly positive), whereas TSLC1 expression intensity ranged from weakly positive to positive in CA, and nearly strongly positive in the normal control group. The differences in the positive expression rate and expression intensity of Wnt-1 and TSLC1 between the two groups were statistically significant (P < 0.05). In addition, Wnt-1 and TSLC1 were negatively correlated. (r = -0.336, P < 0.05). CONCLUSIONS: Overexpression of Wnt-1 and low expression of TSLC1 may be associated with the growth of CA. These findings may provide a basis for the development of therapies to prevent recurrence or malignant transformation of CA.

Apolipoprotein A1 and neuronal nitric oxide synthase gene polymorphisms and hormone-related osteonecrosis of the femoral head.

OBJECTIVE: This study was designed to investigate the influence of several polymorphisms in neuronal NOS genes and apolipoprotein AI on the hormone-related osteonecrosis of the femoral head (ONFH) risk. PATIENTS AND METHODS: Peripheral blood mononuclear cell (PBMCs) samples extracted from hormone-related ONFH patients and controls were used to amplify fragments of the apolipoprotein A1 and neuronal NOS exon 7 and intron 4 using specific PCR primers. The products were analyzed by DNA sequencing and mapped to find the genotype distributions. RESULTS: The proportions of G/G, G/T and T/T on NOS exon 7 in hormone-related ONFH patient group and control group were 68%, 27%, 5% and 81%, 16%, and 3% respectively. The proportions of G/T and T/T in the experimental group were significantly higher than those in the control group (p<0.05). The proportions of b/b, a/b and a/a on NOS intron 4 in hormone-related ONFH patient group and control group were 85%, 13%, 1% and 96%, 5% and 0% respectively. The proportion of a/b in the experimental group was much higher than in the control group. The distribution of A/A, G/A and G/G in the apolipoprotein gene in control and experimental groups were 19%, 33%, 71% and 42%, 20%, 38% respectively. In this case, the experimental group's A/A genotype was significantly higher than the control's genotype. CONCLUSIONS: In our study group, several polymorphisms of the neuronal NOS gene and apolipoprotein A1 genes were significantly associated with hormone-related ONFH. These results suggest that those gene polymorphisms might be involved in the occurrence and development of ONFH.

[Clinical analysis of 41 cases of pulmonary atypical mycobacteriosis].

This paper reported forty-one patients with pulmonary atypical mycobacteriae infection from Jan. 1988 to Oct. 1990 in our hospital. 25 male and 16 female. The age ranged from 27 to 70 years, average 50.9 years. According to Runyon classification of mycobacterium diseases, there are 4 cases with Mycobacterisos kansasii, 2 with M. scrofulaceum, 14 with M. intracellulare or M. avium, 9 with M. chelonae and 12 with M. fortuitum, respectively. Characteristics of this disease were long history, mild symptom and unsatisfactory therapeutic effect by anti-tuberculosis drugs.