Strategies of invasive snail Pomacea canaliculata during hibernation in rice fields of south China: effects of body size, sex, and soil depth.

BACKGROUND: The invasive freshwater snail Pomacea canaliculata is an agricultural pest with a certain level of tolerance to abiotic stress. After the harvest of late rice, the snails usually burrow themselves into the soil surface layers to overwinter and pose a renewed threat to rice production in the following year. Revealing the response of snails to environmental stresses is crucial for developing countermeasures to control their damage and spread. RESULTS: In this study, we conducted a 120-day in situ experiment during the winter to investigate the survival and physiological changes of hibernating snails in 0-5 and 5-10 cm soil depths, aiming to explore their overwintering strategies. Our results showed that 73.61%, 87.50%, and 90.28% of male, female, and juvenile snails survived after hibernation for 120 days in 0-10 cm soil depth, respectively. The differences in survival rates based on sex and size of snails potentially reflect the countermeasures of snails to rapidly reproduce after hibernation. Simultaneously, the hibernating snails exhibited the ability to maintain a certain level of body weight. During this period, the snails increased their antioxidant enzyme activities to cope with oxidative stress, and enhanced their lipid storage. The hibernation survival of snails was not significantly affected by different soil depths, indicating that they have the potential to hibernate into deeper soils. Furthermore, snails were capable of increasing their contents of bound water and glycerol to cope with sudden cold spells during hibernation. CONCLUSION: Our findings emphasize the adaptive changes of P. canaliculata snails overwintering in paddy soils. In future studies, the vulnerabilities of P. canaliculata during hibernation (e.g. shell characteristics, nutrient reserves, and dehydration tolerance, etc.,) should be investigated to develop effective control methods for this period. © 2024 Society of Chemical Industry.

Spatiotemporal characterization of heavy metal and antibiotics in the Pearl River Basin and pollutants removal assessment using invasive species-derived biochars.

Rivers play essential roles in human civilization, while anthropogenic activities have deteriorated their resilience and functionalities. Combating contamination is one of the priorities for building the river's resilience and providing safe water and habitats for livelihoods, wildlife preservation, and food production. We collected 174 water and sediment samples from the upstream to the estuary of the Pearl River (PR), characterized the heavy metal and antibiotics contamination levels, and analyzed the spatiotemporal distribution by compiling historical datasets extracted from published research papers and governmental documents. We also assessed the feasibility of removing PR water heavy metals and antibiotics using biochars derived from two invasive plants, Bidens pilosa L. and Lantana camara. According to our findings, heavy metals and antibiotics in water and sediment increased towards the downstream region of the Pearl River Delta (PRD). The water and sediment samples obtained from the Dongguan and Shenzhen regions exhibited the most elevated levels of heavy metals, whereas the samples from the Huizhou region demonstrated the highest levels of antibiotics. Compared with previously published PRD sediment heavy metals (1976-2011) and antibiotics contamination data (2006-2017), we found that some heavy metals and all measured antibiotics contents in sediment substantially reduced (80-100%). Cu, Zn, Cr, and As significantly polluted the sediment in PRD. Shenzhen had the highest Index of geo-accumulation (Igeo) for Cu, Zn, and Cr, while Zhaoqing had the highest Igeo for As. The dominant antibiotics were Ciprofloxacin, Doxycycline, Norfloxacin, Ofloxacin, Oxytetracycline, and Tetracycline. Invasive plant-derived biochars showed high antibiotic removal capacity but failed to reduce most PR water heavy metals since these invasive plants are potential heavy metal hyperaccumulators. The spatial distribution of heavy metal and antibiotics concentration/content in water and sediment samples is primarily affected by anthropogenic activities such as industrialization, aquaculture, pharmaceutical, and agricultural practice. Our study provides insights into the extensive freshwater watersheds' decontamination and green policymaking.

High-capacity removal of oxytetracycline hydrochloride from wastewater via Mikania micrantha Kunth-derived biochar modified by Zn/Fe-layered double hydroxide.

Antibiotic contamination in water has been an increasing global concern, and how to effectively remove antibiotics (e.g., oxytetracycline [OTC] hydrochloride) from wastewater becomes imperative. In this study, the biochar derived from an invasive plant (Mikania micrantha Kunth) was synthesized with Zn/Fe- layered double hydroxide (LDH) by co-precipitation method (ZnFe-LDH/MBC) to remove OTC from water. ZnFe-LDH/MBC posed the highest OTC removal performance of 426.61 mg/g. ZnFe-LDH/MBC exhibited stability and efficiency in OTC adsorption at different pH levels and under interfering conditions with co-existing ions, as well as outstanding regeneration capabilities during adsorption-desorption cycles. Furthermore, the removal of OTC by ZnFe-LDH/MBC was mediated by several processes including pore filling, hydrogen bonding force, electrostatic interaction, π-π interaction, as well as complexation. Consequently, ZnFe-LDH/MBC has excellent potential for the purification of OTC pollutants that is low-cost, efficient, and environmentally friendly.

Effects of acid rain on plant growth: A meta-analysis.

Anthropogenic driven acid gases emission has caused acid rain in many regions globally. Although efforts have been made to assess the effects of acid rain on terrestrial ecosystems, a systematic assessment of growth-related traits across plant aboveground and belowground is lacking. Hence, we performed a phylogenetically controlled meta-analysis of 755 observations from 69 independent studies to quantify the effects of acid rain on six growth-related traits of plant. We estimated the inhibitory effects of acid rain on plant growth in general and found that aboveground and belowground plant parts responded differently. The acidity of acid rain and acid rain interval had direct modulation effects on plant growth. We also found that there were interactions between acid rain pH and other acid rain characteristics (i.e., acid rain interval, mole ratio of S:N, and acid rain rate) and experimental characteristics (i.e., initial soil pH and plant exposure part), indicating that there were pH-dependent interaction patterns. Thus, an effective approach to evaluate and predict the effects of acid rain on plant growth is to fully consider the direct effects of acid rain pH and the interactions between acid rain pH and other factors.

Simultaneous quantification of the lipids phosphatidylcholine, 3-sn-phosphatidylethanolamine, sphingomyelin, and L-α-lysophosphatidylcholine extracted from the tissues of the invasive golden apple snail (Pomacea canaliculata) using UHPLC-ESI-MS/MS.

Lipids such as phosphatidylcholine (PC), 3-sn-phosphatidylethanolamine (PE), sphingomyelin (SM) and L-α-lysophosphatidylcholine (LPC) are the major components of biological membranes and play important roles in physiological functions. Here, PC, PE, SM, and LPC were extracted from golden apple snails (GAS, Pomacea canaliculata) and GAS flesh (GASF) using an ethanol/hexane sequential scheme and quantified simultaneously using ultra-high performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UHPLC-ESI-MS/MS) to evaluate whether the GAS could be the source of the four lipids. Our results suggest that ethanol extracts contained the most crude lipids, and the yield of dry (evaporated) lipids were 3.45 g per 100 g fresh GASF and 1.82 g per 100 g of fresh GAS. Quantification of the lipids using UHPLC-ESI-MS/MS suggested that GAS contained PE, PC, SM and LPC, with SM being the most abundant lipid (after purification: 1.71 and 1.42 mg g-1 dry weight from 100 g of GASF and GAS, respectively). The method we used is cost-effective, and the recovery rates of ethanol and hexane ranged from 80-91% and 87-91% respectively. Overall, GAS and GASF are potential raw materials for lipids such as SM and PC extraction using the ethanol/hexane method. Comparatively, lipids extraction from the GAS is more effective and timesaving. Our finding would provide a way to utilize GAS and potentially control its invasion.