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Objective:To systematically evaluate the teaching effect of problem-based learning (PBL) plus case-based learning (CBL) versus lecture-based learning (LBL) in surgical internships in Chinese medical students.Methods:We searched PubMed, the Cochrane Library, Embase, China National Knowledge Infrastructure, VIP, and WanFang Data for randomized controlled studies evaluating PBL+CBL versus LBL in surgical internships in Chinese medical students. The retrieval time was from database establishment to June 2019. Data analysis was performed using RevMan 5.2 software.Results:Seventeen randomized controlled trials were included, involving a total of 1161 students, including 581 in the PBL+CBL group and 580 in the LBL group. Meta-analysis results showed that the PBL+CBL group had significantly higher scores of theory examination [mean difference ( MD)=6.15, 95%CI=4.14-8.15, P<0.001], skill examination ( MD=7.67, 95%CI=3.66-11.69, P<0.001), and case analysis ( MD=6.06, 95%CI=2.89-9.23, P=0.000 2) compared with the control group. The PBL+CBL group had a significantly higher percentage of satisfaction with the teaching results than the LBL group [odds ratio( OR)=2.98, 95%CI=1.88-4.73, P<0.001]. Conclusion:PBL+CBL is superior to LBL in medical students' surgical internships, which needs verification by more high-quality studies due to limitations in the number and quality of the included studies.
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Unlike healthy, non-transformed cells, the proteostasis network of cancer cells is taxed to produce proteins involved in tumor development. Cancer cells have a higher dependency on molecular chaperones to maintain proteostasis. The chaperonin T-complex protein ring complex (TRiC) contains eight paralogous subunits (CCT1-8), and assists the folding of as many as 10% of cytosolic proteome. TRiC is essential for the progression of some cancers, but the roles of TRiC subunits in osteosarcoma remain to be explored. Here, we show that CCT4/TRiC is significantly correlated in human osteosarcoma, and plays a critical role in osteosarcoma cell survival. We identify a compound anticarin-β that can specifically bind to and inhibit CCT4. Anticarin-β shows higher selectivity in cancer cells than in normal cells. Mechanistically, anticarin-β potently impedes CCT4-mediated STAT3 maturation. Anticarin-β displays remarkable antitumor efficacy in orthotopic and patient-derived xenograft models of osteosarcoma. Collectively, our data uncover a key role of CCT4 in osteosarcoma, and propose a promising treatment strategy for osteosarcoma by disrupting CCT4 and proteostasis.
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OBJECTIVE: To investigate in vitro antibacterial activity of the extracts from the root, stem and leaves of Psychotria rubra, and to investigate its mechanism of antibacterial effects. METHODS: 95% ethanol extracts from the root, stem and leaves of P. rubra were used to prepare solution with mass concentration of 100 mg/mL (calculated by extract). Using as penicillin sodium (0.5 mg/mL) and streptomycin sulfate (128 mg/mL) as positive control, plat stiletto method was used to determine antibacterial effects of the extracts from different parts of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, Micrococcus luteus and Enterococcus faecalis. Using systematic solvent extraction method, the petroleum ether, ethyl acetate and n-butyl alcohol were used to extract antibacterial activity parts from P. rubra as ad to obtain the extracts of corresponding polar parts. After preparing 100 mg/mL drug solution (calculated by extract), antibacterial effects of above 6 kinds of bacteria were investigated. The micro-broth dilution method and agar culture medium plate method were used to determine minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC), and screen polar parts with bacteriostasis and drug-sensitive strains. Under the concentration of 0.5MIC and MIC, the growth curves of sensitive bacteria were drawn (treated for 36 h, every 4 h); the contents of alkaline phosphatase (AKP) and soluble protein were detected in suspension (treated for 10 h, every 2 h); bouillon culture medium instead of the extract as blank control. RESULTS: 100 mg/mL ethanol extracts from the root, stem and leaves of P. rubra showed good antibacterial effect to above 6 kinds of bacteria, in descending order as stem>leaves>root. Among different polar parts from the stem of P. rubra, antibacterial effect of the ethyl acetate extract was best, especially for the S. aureus, the diameter of inhibition zone reached (38.93±0.12) mm, and both MIC and MBC were 0.39 mg/mL. The ethyl acetate extract could significantly inhibit the proliferation of S. aureus, and its alkaline phosphatase and protein content in suspension were increased significantly compared with blank control (P<0.05). CONCLUSIONS: The stem from P. rubra is effective antibacterial parts, and exhibit good antibacterial activity to 6 kinds of common pathogens. The ethyl acetate extract from the stem of P. rubra shows strongest antibacterial effect on S. aureus, and could gradually destroy the integrity of cell wall and cell membrane.
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OBJECTIVE: To compare inhibitory effects of ethanol extract of different medicinal parts (root, stem, leaf, seed, flower and flesh) from Syzygium jambos on the activities of α-glycosidase and α-amylase. METHODS: Using half-inhibitory concentration value (IC50) as evaluation index, acarbose as positive control, inhibitory effects of ethanol extract of different medicinal parts from S. jambos on the activities of α-glycosidase (from yeast and small instestine in mice) and α-amylase were evaluated with in vitro inhibition model. The enzymatic dynamics and Lineweaver-Burk methods were used to analyze the inhibitory type of the best medicinal part on the activities of α-glycosidase and α-amylase. RESULTS: In the yeast α-glucosidase inhibitory activity test, the order of inhibitory activity was S. jambos seed>S. jambos stem>S. jambos leaf>S. jambos root>S. jambos flower>S. jambos flesh>acarbose. In the mice intestine α-glucosidase inhibitory activity test, the order of inhibitory activity was S. jambos seed>S. jambos stem>S. jambos root>S. jambos leaf>S. jambos flower>S. jambos flesh>acarbose. In the α-amylase inhibitory activity test, the order of inhibitory activity was acarbose>S. jambos seed>S. jambos stem>S. jambos root>S. jambos leaf>S. jambos flesh>S. jambos flower. Ethanol extract of S. jambos seed had the stronger inhibition activity against α-glucosidase from yeast,α-glucosidase from small intestine in mice and α-amylase than other medicinal parts [IC50 were(6.64±0.24), (32.77±2.46) and (41.18±1.63) μg/mL]. Ethanol extract of S. jambos seed had the stronger inhibition activity against α-glucosidase than acarbose [IC50 to α-glucosidase from yeast and α-glucosidase from small intestine in mice were (2 833.33±5.48), (1 304.21±6.45) μg/mL] (P<0.05). The inhibitory effect of ethanol extract from S. jambos on the activity of α-amylase was less than that of acarbose [IC50 was (27.27±1.24) μg/mL] (P<0.05). Enzymatic dynamics showed that the inhibitory type of ethanol extract from S. jambos seed on α-glucosidase and α-amylase were both reversible competitive inhibition. CONCLUSIONS: Among different parts of S. jambos such as root, stem, leaf, seed, flower and flesh, S. jambos seed shows the strongest inhibitory effects on the activities of α-glucosidase and α-amylase, which has the value of being developed for the treatment of diabetes or health food.
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Objective@#To investigate the changes in clinical characteristics and laboratory indexes before and after the termination of pregnancy in patients with acute fatty liver of pregnancy (AFLP).@*Methods@#Patients with acute fatty liver of pregnancy who had been admitted to the Department of Obstetrics and Gynecology at the Second Affiliated Hospital of Chongqing Medical University and Chongqing Municipal People’s Hospital of Jiangbei District between 2007 and 2018 were selected. Clinical characteristics and complications during diagnosis and treatment, changes in blood coagulation, liver and kidney function, and postpartum recovery were collected for retrospectively analysis.@*Results@#54 cases with average gestational age of 35.0±1.7 weeks at third trimester of pregnancy with AFLP were treated. The most common gastrointestinal symptoms were yellow urine, nausea and vomiting. All patients had elevated bilirubin. 90.7% patients had changes in blood coagulation function and 68.5% had elevated serum creatinine. Transaminase levels were dropped rapidly within 1-2 days after the termination of pregnancy. Total bilirubin recovery was slow and partially recovered after 6-8 days. Serum creatinine and BUN increased slightly after delivery, reaching a peak at 3-4 days and then began to deplete. There was slight change in prothrombin time and fibrinogen after delivery, but returned to normal level after 5-6 days. The most common complications were AKI (74.1%), LF (42.6%), PPH (40.7%) and DIC (33.3%). Twenty-three of the 54 cases (42.6%) progressed to acute liver failure. AFLP complicated with ALF course was significantly longer than healthy controls, and the disease severity was significantly increased, with a mortality rate of 17.4% (4/23), and 0 in healthy controls. The difference was statistically significant.@*Conclusion@#Early diagnosis and termination of pregnancy are the key factors to determine the prognosis of pregnant patients with acute fatty liver. Blood coagulation function does not deteriorate after termination of pregnancy and renal function begins to recover after 4 days with slight restoration of liver function. The control of complications is an important factor to determine the prognosis of patients.
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The neuroimaging provides a lot of useful information about the neuromechanism of restless legs syndrome (RLS).The voxel-based morphometry and diffusion tensor imaging findings reported brain microstructural abnormalities,which were more consistent concerning levels of white matter and belonged to the sensorimotor and limbic system.Functional magnetic resonance imaging (MRI) studies also demonstrated the neural activity and/or functional connectivity changes in the sensorimotor and limbic network.Positron emission tomography and single photon emission computed tomography studies supported the dysfunction of the dopaminergic pathways involving not only the nigrostriatal but also mesolimbic pathways.Iron-sensitive MRI verified low brain iron content mainly in substantia nigra and thalamus.The primary change might be the reduction of brain iron content,which leads to the dysfunction of the nigrostriatal and mesolimbic dopaminergic pathways and in turn to a dysregulation of sensorimotor and limbic network resulting in the symptoms of RLS.
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Objective To evaluate the clinical efficacy and safety of Chaigan Jieyou decoction in treating Parkinson's disease depression (PDD).Methods Applying the method of simple random contrast,this research collects 70 patients who meet the study criteria and divide the patients into two groups of 35 peoples each:Chaigan Jieyou decoction group,which received treatment of Chaigan Jieyou decoction and fluoxetine group with treatment of fluoxetine.Treatment period was 2 months.Apply the HAMD-17 (Hamilton Depression Scale),Beck Depression Scale (Beck Depression Inventory,BDI),Parkinson's composite score (unified Parkinson disease rating scale,UPDRS) and TCM symptoms rating scale to evaluate the clinical efficacy before and after treatment.Resuits Compared the two groups by HAMD Score reduction rate,there is no statistical significance in the general efficacy rate (P > 0.05);compared by the curative effect of TCM syndrome,Chaigan Jieyou decoction group is better than fluoxetine group (P < 0.05).There is statistical significance in the differences of all the HAMD,BDI,UPDRS Ⅱ,UPDRS Ⅲ scores between the two groups after the treatment (P < 0.01,P < 0.05).Compared the treatment efficacy by the HAMD scores,Chaigan Jieyou decoction can alleviate the clinical symptoms of patients of varying degrees.Conclusions Chaigan Jieyou decoction can significantly relieve depression of PDD patients,and there is exact curative effect to the mild and moderate PDD patients,improving their quality of life to a certain extent.
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This study preliminarily discussed the TCM syndrome types of myasthenia gravis (or flaccidity) and its treatments and prescriptions through consensus method,providing evidence and level revision of the recommended expert consensus of myasthenia gravis in TCM clinical practice guideline.Literatures over TCM syndrome types,symptoms,therapeutic regime,medication and acupuncture were involved in the syndrome analysis of myasthenia gravis through literature retrieval.The common clinical syndromes were outputted by mentioned rate.Ten first-rate hospitals were finally included,such as The Affiliated Hospital of Changchun University of Traditional Chinese Medicine,Dongfang Hospital of Beijing University of Chinese Medicine,Guangdong Province Traditional Chinese Medical Hospital and Affiliated Hospital of Anhui University of Traditional Chinese Medicine.Twelve experts in aggregate were confirmed to participate in the discussion for the identification of the TCM syndrome types,treatments and prescriptions of myasthenia gravis.As a result,five TCM syndrome types,such as syndrome of spleen and stomach qi deficiency,syndrome of spleen-kidney deficiency,deficiency syndrome of both qi and yin,syndrome of deficiency and sinking of qi,were confirmed;while five therapeutic regimes were approved,including reinforcement of qi,tonification of spleen and stomach,tonification of spleen and kidney qi,tonification of qi and yin,the supplement of qi and restoration of yang for ascending qi collapse.In addition,five prescriptions were supported by the experts,such as Bu Zhong Yi Qi (BZYQ) decoction,BZYQ decoction combined with You Gui pills,BZYQ decoction combined with Sheng Mai powders and Sheng Xian decoction.In conclusion,the expert consensus method for the screen of TCM syndrome types of myasthenia gravis embodied the conception of literature research based on the clinical practice combined with the scientific methods in conformity with the current research approaches to TCM syndrome types.
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Objective To investigate the clinical efficacy of buttress plating for patients with posterior pilon fracture.Methods From April 2012 to January 2015,12 patients with posterior pilon fracture of the distal tibia were treated in our hospital.They were 7 men and 5 women,30 to 56 years of age (average,41.2 years).According to the CT classification by Haraguchi et al.,5 cases belonged to type I,3 to type Ⅱ and 4 to type Ⅲ.All the patients underwent open reduction and internal fixation with buttress plate via either a posterolateral approach or a dual posterolateral-posteromedial approach.All the patients were available for follow-up.The clinical outcomes were evaluated using the American Orthopaedic Foot and Ankle Society (AOFAS) ankle-hindfoot score and the visual analogue scale (VAS).The radiological evaluation was performed using the osteoarthritis-score (OA-score).Results The patients obtained an average follow-up of 21.2 months (range,from 12 to 30 months).Bone fractures united after an average of 15 weeks (range,from 13 to 19 weeks).The time for full weight walking averaged 16 weeks,ranging from 15 to 23 weeks.The ankle plantar flexion ranged from 36° to 42° (average,40.4°);the ankle dorsal extension ranged from 38° to 44° (average,42.6°).At the final follow-ups,the AOFAS scores ranged from 82 to 97 (average,88.2);the OA-score ranged from 0.6 to 0.8 (average,0.71);the VAS scores during rest,active motion and weight-bearing walking ranged from 0.5 to 0.8 (average,0.66),from 0.6 to 0.9 (average,0.82) and from 1.2 to 1.8 (average,1.41),respectively.No fracture malunion,implant loosening,pain or stiffness of the affected ankle was observed at the final follow-ups.Conclusion Buttress plating for posterior pilon fractures can lead to satisfactory clinical outcomes,because it ensures rigid fixation which in turn enables earlier postoperative mobilization.
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Regional hypoxia caused by accelerated cell proliferation and overgrowth is an important characteristic of neoplasm. Hypoxia can cause a series of changes in gene transcription and protein expression, thereby not only inducing tumor cell resistance to radiotherapy and chemotherapy but also promoting tumor invasion and metastasis. This study aimed to investigate the relationship between HIF-1α expression and cellular apoptosis, angiogenesis and clinical prognosis in rectal carcinoma. In 113 rectal carcinoma cases, cellular apoptosis was analyzed by the in situ terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) assay, whereas the levels of HIF-1α expression, VEGF expression, microvessel density (MVD) and lymphatic vessel density(LVD) were examined by immunohistochemical staining. HIF-1 expression was detected in 67 of 113 rectal carcinoma cases (59.3 %). A positive correlation was found among HIF-1α expression, cellular apoptosis and angiogenesis. The 5-year survival rate in the HIF-1α-negative group was significantly higher than that in the HIF-1α-positive group (81.34 % versus 50 %, P < 0.05). According to the Cox regression analysis, HIF-1α expression, VEGF expression and cellular apoptosis index were independent risk factors for clinical prognosis in rectal carcinoma. Aberrant HIF-1α expression correlates with apoptosis inhibition, angiogenesis and poor prognosis in rectal carcinoma.
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Apoptose , Biomarcadores Tumorais/metabolismo , Subunidade alfa do Fator 1 Induzível por Hipóxia/metabolismo , Microvasos/patologia , Neovascularização Patológica/metabolismo , Neoplasias Retais/metabolismo , Neoplasias Retais/patologia , Feminino , Seguimentos , Humanos , Técnicas Imunoenzimáticas , Metástase Linfática , Masculino , Microvasos/metabolismo , Pessoa de Meia-Idade , Estadiamento de Neoplasias , Prognóstico , Neoplasias Retais/irrigação sanguínea , Neoplasias Retais/mortalidade , Taxa de Sobrevida , Fator A de Crescimento do Endotélio Vascular/metabolismoRESUMO
Objective To investigate the interaciton of automatic thoughts and stress factors related depression in fe-male patients with first-episode depressive disorder. Methods The 60 female patients with first-episode depression were enrolled and assessed by Hamilton Depression Rating Scale(HAMD),Hamilton anxiety scale(HAMA),Automatic thought questionnaire (ATQ), Dysfunctional attitude scale (DAS),ways of coping, the social support scale and Y-G Personality Questionnaire. Spearman correlation analysis and path analysis were used to analyse the interaciton of auto-matic thoughts and stress factors related depression. Results ①Automatic thoughts, depression,anxiety and dysfunc-tional cognition were positively correlated each other. Automatic thoughts and vulnerability, attraction and repulsion ,perfection and autonomy attitude were positively correlated (P<0.05 or P<0.01). ②Automatic thinking and feeling of inferiority, nervosity, lack of objectivity and non cooperative were positively correlated,while dominant, social in-side and outside were negatively correlated. The differences were statistically significant(P<0.05 or P< 0.01). ③Au-tomatic thoughts and blame, rationalization were positively correlated, while automatic thoughts, total score of social support and subjective support were negatively correlated. ④Path analysis showed that automatic thoughts predicted depression directly; nervosity, the perfection, blame, anxiety predicted depression indirectly through the automatic thoughts; subjective support acted on automatic thinking as negatively predicator, and predicted depression indirectly through the automatic thoughts (P<0.05 or P<0.01). Conclusion Interaction existed among automatic thoughts and stress factors of depression related. When making the psychological treatment of depression,we should be considered these factors.
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<p><b>OBJECTIVE</b>To evaluate the influence of liver function on pregnancy outcome of women who are infected with the hepatitis B virus (HBV) and who receive individualized nutrition management during the perinatal period.</p><p><b>METHODS</b>A total of 210 pregnant women were enrolled for study between January 2011 and July 2013. The women were divided into the following groups according to self-selected participation in individualized nutritional treatment and management plans (n =87, observation group) or in routine prenatal care without any nutrition management plan (n =123, control group). Inter-group differences in pregnancy complications, liver function and perinatal outcome were assessed statistically.</p><p><b>RESULTS</b>The following pregnancy complications were recorded during the study:hypertension disorder, gestational diabetes, anemia and fetal growth restriction; however, the rates of these complications were significantly lower in the observation group than in the control group (P less than 0.05). The observation group also had significantly lower incidence rates of preterm labor, fetal distress and neonatal asphyxia (P less than 0.05). The observation group also had significantly lower proportions of hypoalbuminemia and abnormal liver function, and correlation analysis indicated that birth weight is correlated with the pregnant woman's energy and protein intake.</p><p><b>CONCLUSION</b>Women with HBV can benefit from individualized nutrition management during the prenatal period, experiencing improved pregnancy outcome and reduced occurrence of pregnancy complications. The application of individualized and balanced nutrition management had no effect on fetal growth and development.</p>
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Adulto , Feminino , Humanos , Gravidez , Adulto Jovem , Portador Sadio , Hepatite B , Terapêutica , Testes de Função Hepática , Terapia Nutricional , Métodos , Complicações na Gravidez , Epidemiologia , Resultado da GravidezRESUMO
This article was aimed to study the protection effects of Pa-Bing Formula No. 2 (PBFN-2) on neurons of substantia nigra in Parkinson's disease (PD) rats models in order to explore the possible mechanism of PBFN-2 in PD treatment. Rats were stereotaxically injected with 6-hydroxydopamine (6-OHDA) solution into the left stria-tum in two-site. Rat showed consistent right whirling and the number of rotation was more than 7 r·min-1 induced by APO in 30 min, then the rat was judged as PD model. A total of 14 rats modeled successfully were randomly di-vided into the model group and the treatment group. At the same time, the normal group and sham operation group were also established. Same volume of distilled water was given to the normal group, sham operation group and the model group. PBFN-2 (32.0 g·kg-1) was given to the treatment group for 4 weeks. Microscope was used to observe pathological changes of substantia nigra by nissl stain and changes of TH, Nrf2 and HO-1 immunohistochemical stain. The results showed that compared with the normal group, the nissl bodies were badly injured. Expressions of TH-positive cells were obviously reduced (P<0.01). The expression of Nrf2 nucleus protein and HO-1 protein were obviously increased in substantia nigra of PD rats in model group (P<0.01). Compared to the model group, PBFN-2 effectively increased nissl bodies in neuronal cells of substantia nigra of PD rats, and elevated the number of TH-immunoreactive cells in substantia nigra (P<0.05). The expressions of Nrf2 nucleus protein, HO-1 protein were significantly up-regulated (P<0.05). It was concluded that PBFN-2 had an obvious neuroprotection on the neuronal cells in substantia nigra of PD rats induced by 6-OHDA. The underlying mechanisms may be associated with regu-lation of Nrf2 nucleus protein and HO-1 protein expressions.
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Objective To build rat models and observe changes of behavior and monoamine of PDD. Methods The rats were injected with 6-OHDA and accepted CUMS for the establishment of PDD model , so as to assess its behavior changes and detect brain tissue monoamine contents by ELISA. Results Significant behavioral and monoamine abnormalities can be observed in model rats. Behavioral and monoamine improvement can be observed after treated with Chaigan Jieyou. Conclusion The PDD model can be established by 6-OHDA and CUMS. Chaigan Jieyou decoction may reach therapeutic purposes by increasing monoamine contents.
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Parkinson's disease (PD) is a commonly encountered central neurodegenerative disease in elderly people. According to theories of traditional Chinese medicine (TCM), PD is characterized by deficiency in the root and excess in the branch. Deficiency was referred to qi-blood deficiency of the liver and kidney. Excess was referred as the wind, fire, phlegm and stasis. Deficiency was the root and excess was the branch. Good efficacies have been obtained by treatment based on syndrome differentiation, treatment with specific prescriptions, acupuncture and moxibustion and comprehensive treatment. Some experiments had been conducted to elucidate its mechanisms. However, no uni-fied standard for therapeutic evaluation, poor control of medicinal quality, inferior quality of designed clinical trials, and unclear treatment mechanism in the clinical study on therapeutic effect of PD treatment with TCM require fur-ther studied.
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Objective The purpose of this study was to compare chemerin and CRP levels between women with and without ear‐ly‐onset preeclampsia based on maternal body mass index (BMI) .Methods 45 patients with early onset preeclampsia (gestation week is less than 34 weeks) ,30 normal pregnancy women were enrolled in the study .The serums levels of chemerin and CRP were measured in these pregnancy women .Results The chemerin [(379 .18 ± 52 .09)ng/L ,(481 .27 ± 63 .12)ng/L] and CRP [(7 .59 ± 1 .41)mg/L ,(11 .74 ± 1 .61)mg/L] levels in MPE and SPE were significantly higher than those [(316 .08 ± 51 .90)ng/L ,(3 .47 ± 1 .27)mg/L]in normal pregnancy women(P<0 .05) .While the chemerin(424 .64 ± 46 .61)ng/L and CRP (8 .32 ± 3 .82)mg/L levels increased with maternal BMI in patients overweight and obese women levels compared with normal weight women [(331 .66 ± 51 .77)ng/L ,(5 .93 ± 2 .95)mg/L] ,the difference are significant(P<0 .05) .The correlation analysis showed that the chemerin were positively correlated with BMI ,systolic blood pressure ,diastolic blood pressure ,triglycerides ,and CRP were positively correlated with BMI .Conclusion The expression of chemerin and CRP were associated with severity of preeclampsia and BMI .
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Objective To investigate the effect of dexcedetomidine on the postoperative delirium in adolescent idiopathic scoliosis undergoing spine surgery. Methods 160 patients (7-16 years of age) with idiopathic scoliosis undergoing posterior spinal fusion were randomly divided in to two groups. Group SS (n=80) included patients anesthetized with sevorane and sufentanil, group SSD (n=80) included patients anesthetized with sevorane,sufentanil and dexcedetomidine. In the latter group, dexcedetomidine was administered as a continuous infusion of 0.2 ug/h started after the induction of anesthesia without a loading dose. Sevorane was adjusted to maintain the bispectral index (BIS) number at 40-60 and vasoactive drugs was adjusted to maintain the mean arterial pressure (MAP) at 65-75 mmHg. The amount of anesthetic, the time of anesthesia recovery,restlessness during recovery and postoperative delirium were recorded. Results The amount of sevorane, the incidence of restlessness during recovery and the incidence of postoperative delirium were significantly lower in the group SSD than in group SS ( <0.05) . Conclusion The perioperative infusion of 0.2 ug/h dexcedetomidine can decrease the incidence of postoperative delirium in adolescent idiopathic scoliosis undergoing spine surgery.
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Objective To evaluate the efficacy and safety of brachytherapy by percutaneous paracentesis implantation of iodine-125 seeds for elderly non-small cell lung cancer.Methods 18 patients with peripheral lung cancer were undergone brachytherapy by percutaneous paracentesis implantation of iodine-125 seeds.The effect was evaluated by periodically chest CT.The complications were analyzed.Results 18 patients were observed one,two and six months after operation.CR,PR,SD were seen in 27.8%(3/18),66.7%(14/18),5.6%(1/18)respectively of the patients.Pneumothorax,hemoptysis were seen in 16.67%(3/18),95.37%(17/18),no pleural cavity bleeding.Conclusion Brachytherapy by percutaneous paracentesis implantation of iodine-125 seeds for lung cancer was effecitve.The complications were easy to treat.The methods of implantation of iodine-125 seeds could fully play the advantages of CT,and reduce the damage to the body when raising the tumorous control rate.
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The main clinical symptoms of Parkinson's disease (PD) are resting tremor, muscle rigidity, bradykinesia, and so on. There is no effective treatment for PD yet, and dyskinesia symptoms affect the life qualities of PD patients. The therapy used for reinforcing kidney and activating blood circulation in treatment of PD can achieve good clinical effects.
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This was a study aimed to observe the proliferating ability inhibited by energy controllable steep pulse (ECSP) and to detect the expression of gene with relation to the proliferating ability of the tumor in breast cancer cell line; the possible mechanisms were also addressed. Human breast cancer cell line MDA-MB-231 was treated with ECSP; the apoptosis and the expression of tumor suppressor gene--Rb genes and E2F1 genes in ECSP group and control group were detected by TUNEL staining and Reverse Transcripitional PCR respectively. ECSP was found to inhibit the proliferating ability of breast cancer cells markedly, the cell amount in ECSP group decreased and the TUNEL positive cells increased obviously, compared to control; 24 hours after treatment the expression of Rb genes mRNA increased, whereas the expression of E2F1 mRNA decreased. These findings indicate that the proliferating ability of breast cancer cells can be inhibited by ECSP markedly, the apoptosis of breast cancer cell can be induced by ECSP, and the Rb genes and E2F1 genes may be involved in the course.