Open Reading Frame-3a gene of the 2019 novel coronavirus inhibits the occurrence and development of colorectal cancer.

Intestinal microecology is composed of bacteria, fungi and viruses. As a part of intestinal microecology, viruses participate in the occurrence and development of colorectal cancer. The 2019-nCoV was detected in stool samples from patients during COVID-19, suggesting that the 2019-nCoV may be associated with intestinal microecology. However, the relationship of the 2019-nCoV and CRC is unclear. The aim of this study is to explore the role of Open Reading Frame-3a (ORF3a) of the 2019-nCoV in CRC. After the pCDH-CMV-MCS-EF1-Puro vector that provides high expression of ORF3a was transfected into the SW480 CRC cell line, immunofluorescence was used to determine the localization of ORF3a in SW480 cells. The proliferation, migration, invasion, apoptosis, and cell cycle progression of SW480 cells were measured using the Cell Counting Kit-8 (CCK-8), wound healing, Transwell assay, flow cytometry, the TUNEL assay, and propidium iodide single staining. The results showed that ORF3a inhibited the proliferation, invasion, and migration of SW480 cells and induced their apoptosis after 24, 48, 72 h. Meanwhile, ORF3a inhibited the cell cycle and blocked SW480 CRC cells in the G1 phase. In in vivo experiments, high ORF3a expression was associated with decreased tumor volume, tumor weight, relative tumor volume, and tumor activity. ORF3a inhibited the growth and induced apoptosis and necrosis of tumor tissues. Based on this, we demonstrated that ORF3a might play a role in CRC, providing a new direction for the prevention and treatment of CRC.

Mechanisms of induction of tumors by cholesterol and potential therapeutic prospects.

Increasing evidence suggested that cholesterol is an important integrant of cell membranes, that plays a key role in tumor progression, immune dysregulation, and pathological changes in epigenetic mechanisms. Based on these theories, there is a growing interest on targeting cholesterol in the treatment of cancer. Here, we comprehensively reviewed the major function of cholesterol on oncogenicity, the therapeutic targets of cholesterol and its metabolites in cancer, and provide detailed insight into the essential roles of cholesterol in mediating immune and epigenetic mechanisms of the tumor microenvironment. It is also worth mentioning that the gut microbiome is an indispensable component of cancer mediation because of its role in cholesterol metabolism. Finally, we summarized recent studies on the potential targets of cholesterol and their metabolism, to provide more therapeutic interventions in oncology.

Differences in T cell immune-related lncRNA and mRNA expression patterns between right- and left-sided colorectal cancers.

BACKGROUND: Right-sided colorectal cancer (RCRC) and left-sided colorectal cancer (LCRC) harbor different genetic alterations associated with immune response. OBJECTIVE: This study aimed to analyze the differences in T cell immune-related RNA expression patterns between RCRC and LCRC. METHODS: The differentially expressed genes (DEGs) and lncRNAs (DElncRNAs) between LCRC and RCRC were screened from the Cancer Genome Atlas (TCGA) database. A correlation analysis between DEGs or DElncRNAs and differential T cells was also performed to obtain T cell-related genes, followed by miRNA prediction. The mRNA-lncRNA network and the competitive endogenous RNA (ceRNA) network were subsequently constructed, and the expression level of mRNA in the ceRNA network was verified using GSE104645. RESULTS: RCRC patients had a poorer prognosis and were older than LCRC patients. In total, 923 DEGs and 328 DElncRNAs were screened between LCRC and RCRC patients. Compared to RCRC patients, LCRC patients showed a decrease in CD8+ T cells. In addition, 26 miRNAs, 8 mRNAs, and 10 lncRNAs were included in the ceRNA network. Finally, the validation analysis revealed that CDHR1 and PRLR were significantly downregulated, while TRIB2 was upregulated in RCRC patients compared to LCRC patients. CONCLUSION: The analysis of T cell immune-related RNA expression might provide new insights into the underlying molecular mechanisms of the differences between LCRC and RCRC.

Advance of Aquatic Therapy for Autistic Children (review) / 中国康复理论与实践

The aquatic therapy is a part of comprehensive rehabilitation. Aquatic therapy may benefit the social interaction, stereotyp-ing and motor learning of children with autism, which may associate with the physical and mental mechanism. The programs applied for the autism include game in water, swimming, physical fitness training in water, etc. The assessment tools include scales and video, and so on.

Role of Swimming in Rehabilitation to School-age Children with Cerebral Palsy / 中国康复理论与实践

@#Objective To observe the effects of swimming on school-age children with cerebral palsy. MethodsHalliwick swimming method was used in swimming group (n=16), while the control group (n=15) received routine training. Before and after training, they were assessed with the Gross Motor Function Measure (GMFM-88) and the Berg Balance Scale. ResultsMost of the swimming group mastered swimming skills. The total scores of GMFM-88 and the Berg Balance Scale scores improved significantly in the swimming group (P<0.05), but not significantly in the control group (P>0.05) after training. ConclusionHalliwick technique is an effective way to learn swimming for school-age children with cerebral palsy. There is certain effect of swimming on motor function, especially on the balance functions.

Urban-rural disparity of overweight, hypertension, undiagnosed hypertension, and untreated hypertension in China.

The objective of this study was to examine the urban-rural variations in the prevalence of overweight, hypertension, undiagnosed hypertension, and untreated hypertension among adults. The China Health and Nutrition Survey was used to study adults 25 to 65 years old. Logistic regression was used to obtain the odds ratios (ORs) after adjusting for demographic, socioeconomic, and lifestyle variables. Urban adults have a higher probability of being overweight (OR = 1.18, P < .01) and having hypertension (OR = 1.19, P < .1). After further controlling for lifestyle variables, the difference is not significant. Urban hypertensive adults are less likely to be undiagnosed (OR = 0.54, P < .001) and untreated (OR = 0.53, P < .001), even after controlling for socioeconomic and lifestyle variables, suggesting that there are other reasons for the urban-rural disparity of undiagnosed and untreated hypertension. This finding suggests that modifying lifestyles is critical in preventing urban adults from being overweight. Interventions targeting rural adults should promote the awareness and treatment of hypertension.