Utilization of Drivers' Dynamic Visual Characteristics to Find the Appropriate Information Quantity of Traffic Engineering Facilities on Straight Roads of Grassland Highways.

To find the appropriate range of information quantity, we studied how the information quantity of traffic engineering facilities (TEFs) on straight roads of grassland highways affects a driver's eye movements. We used a combination of survey, statistics, analysis of variance, and the establishment of virtual scene to do this research, and carried out simulated driving tests at six levels (Z0, Z1, Z2, Z3, Z4, and Z5) of TEF information quantity. The driver's fixation duration, visual search breadth, and glance speed were evaluated in a quantitative way. Results showed that the information quantity had a significant impact on eye movements. It is concluded that the information quantity from 0 to 10 bits/km may cause problems to drivers, whereas the information quantity of 40 bits/km serves as the limit. The information quantity from 30 to 40 bits/km is the appropriate one for TEF on grassland highways.

Carrier ampholyte-free isoelectric focusing on a paper-based analytical device for the fractionation of proteins.

Isoelectric focusing plays a critical role in the analysis of complex protein samples. Conventionally, isoelectric focusing is implemented with carrier ampholytes in capillary or immobilized pH gradient gel. In this study, we successfully exhibited a carrier ampholyte-free isoelectric focusing on paper-based analytical device. Proof of the concept was visually demonstrated with color model proteins. Experimental results showed that not only a pH gradient was well established along the open paper fluidic channel as confirmed by pH indicator strip, the pH gradient range could also be tuned by the catholyte or anolyte. Furthermore, the isoelectric focusing fractions from the paper channel can be directly cut and recovered into solutions for post analysis with sodium dodecyl sulfate-polyacrylamide gel electrophoresis and matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry. This paper-based isoelectric focusing method is fast, cheap, simple and easy to operate, and could potentially be used as a cost-effective protein sample clean-up method for target protein analysis with mass spectrometry.