RESUMO
Adipic acid diselenoester was developed as an efficient cross-linker for covalent protein conjugation with a variety of small molecular haptens, including mono- and disaccharides, peptide, fluorescence dye, and nicotine. Compared to the counterparts of N-hydroxysuccinimide (NHS) and p-nitrophenyl (PNP) linkers, the diselenoester linker demonstrates improved balance between reactivity and stability and coupling of haptens to proteins under mild conditions with high incorporation efficiency.
Assuntos
Adipatos/química , Reagentes de Ligações Cruzadas/química , Haptenos/química , Compostos Organosselênicos/química , Ovalbumina/química , Soroalbumina Bovina/química , Adipatos/síntese química , Reagentes de Ligações Cruzadas/síntese química , História do Século XX , Compostos Organosselênicos/síntese química , Vacinas Sintéticas/químicaRESUMO
An efficient N-linked glycosylation reaction between glycosylamines and p-nitrophenyl thioester peptides has been developed. The reaction conditions are mild and compatible with the C-terminal free carboxylic acid group and the unprotected N-linked sialyloligosaccharide. By means of this convergent strategy, a versatile N-glycopeptide fragment containing an N-terminal Thz and a C-terminal thioester was readily prepared, which is available for the synthesis of long glycopeptides and glycoproteins using the protocol of native chemical ligation.
