RESUMO
BACKGROUND:Exercise has a regulatory effect on intestinal flora dysbiosis,which can effectively protect the beneficial flora and improve the intestinal environment.However,the effect of treadmill exercise on the structure and diversity of intestinal microbial community in Parkinson's disease and the specific mechanism are not clear. OBJECTIVE:Using 16S rDNA technique to analyze the effect of treadmill exercise on the structure and diversity of the intestinal flora of rats with Parkinson's disease,and to investigate the mechanism of non-pharmacological treadmill exercise to improve Parkinson's disease. METHODS:Twelve of the 18 Sprague-Dawley rats were randomly selected to make animal models of Parkinson's disease using unilateral 2-point nigrostriatal injection of 6-hydroxydopamine.The remaining six rats were used as sham-operation group,which were injected with the same dose of saline containing 0.2%ascorbic acid using the same positioning and injection method.After successful modeling,12 rats with Parkinson's disease were randomly divided into model group and treadmill exercise group(n=6 per group).The treadmill exercise group was subjected to a middle and low intensity tread mill exercise,10 m/min,30 minutes per day,5 days per week for 4 weeks.Fresh feces were collected and stored in liquid nitrogen 24 hour after the last exercise session,and the changes in fecal flora were analyzed by 16S rDNA sequencing technique. RESULTS AND CONCLUSION:Treadmill exercise significantly improved behavior and nigrostriatal tyrosine hydroxylase-positive cell expression in rats with Parkinson's disease model and alleviated changes in the structure and diversity of the gut microbial community caused by Parkinson's disease,increased the number of operational taxonomic units and modulated Alpha and Beta diversity in rats.At the phylum and genus levels,the abundance ratio of Firmicutes/Bacteroidetes in the model group decreased compared with the sham-operated group,while beneficial bacteria such as Prevotella,Bacteroides,and Clostridium_XlV increased significantly after treadmill exercise.To conclude,treadmill exercise has a significant modulating effect on behavioral abnormalities,toxic damage to dopaminergic neurons and gut microbial imbalance caused by Parkinson's disease,alleviates the symptoms of flora-related diseases,and has a positive effect on the improvement of Parkinson's disease.
RESUMO
OBJECTIVE: To analyze aging-related regional changes in the lower eyelid tissue structure of Han Chinese women as data reference and theoretical basis for rejuvenation treatment of lower eyelids. METHODS: Magnetic resonance imaging (MRI) data of healthy Han Chinese women with a body mass index (BMI) between 18.5-23.9 kg/m2 were collected and stratified by age. MRI of the mid-sagittal plane of the eyeball was analyzed. The orbital margin was marked as the coordinate O point while the surface of the zygomatic bone was marked as the coordinate y-axis. The vertical line between the O point and y-axis was marked as the x-axis. The position of each point of the orbicularis oculi muscle contour and the subcutaneous fat tissue contour on the coordinate axis was measured. The thickness and contour morphology of the subcutaneous fat and orbicularis oculi muscle at different positions were calculated according to the coordinate position of each point. Age-related changes in the structure, contour, and position of the subcutaneous tissue in the lower eyelid area were analyzed. RESULTS: With aging, the thickness of the orbicularis oculi muscle decreased while its width increased. Notably, the radian of the orbicularis oculi muscle in the tarsal portion protruding to the skin increased. The subcutaneous fat of the orbital margin thinned while the subcutaneous fat below the orbital margin thickened with age. CONCLUSION: The subcutaneous tissue structure of the lower eyelid region in Han Chinese women showed regular changes during aging, chiefly characterized by the widening and thinning of the orbicularis oculi muscle, and the gradual shifting of the subcutaneous fat to the middle and lower regions.