RESUMO
AIM: To study the relationship between the cyclooxygenase (COX)-2 gene and the proliferation and apoptosis of esophageal squamous carcinoma EC109 cells. METHODS: The techniques of RNA interference (RNAi) and cell transfection, as well as the levels of oncogenicity in nude mice, were used to study the role of COX-2 in the esophageal squamous carcinoma cell (ESCC) line EC109. Following RNAi and transfection, Western blotting analysis was used to determine the expression of the COX-2 protein. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) reduction assay was used to evaluate cell growth, and flow cytometry was used to detect cell apoptosis. RESULTS: Western blotting analysis demonstrated that COX-2 expression was significantly reduced in EC109 cells treated with COX-2-specific short interfering RNA (siRNA) but was increased in EC109 cells transfected with COX-2. Furthermore, COX-2 siRNA treatment inhibited cell proliferation (P < 0.01) and induced apoptosis in EC109 cells, as determined by an MTT assay and by flow cytometry, respectively. In contrast, transfected COX-2 led to increased cell proliferation (P < 0.05) and decreased apoptosis in EC109 cells. In addition, combination treatment of cells with COX-2 siRNA and aspirin had a synergistic effect (P < 0.01). For experiments measuring tumorigenicity, xenograft tumors of a greater volume and weight were found in the COX-2 group compared with other groups (P < 0.05). A large dose of aspirin inhibited tumor growth in nude mice effectively (P < 0.05), and the rate of tumor suppression was 51.8% in the high-dose aspirin group. CONCLUSION: COX-2 plays a very critical role in ESCC carcinogenesis, and COX-2 siRNA combined with aspirin has the potential to be an anticancer therapy for the treatment of ESCC.
Assuntos
Carcinoma de Células Escamosas/enzimologia , Ciclo-Oxigenase 2/metabolismo , Neoplasias Esofágicas/enzimologia , Animais , Apoptose/efeitos dos fármacos , Aspirina/farmacologia , Carcinoma de Células Escamosas/genética , Carcinoma de Células Escamosas/patologia , Carcinoma de Células Escamosas/fisiopatologia , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Ciclo-Oxigenase 2/genética , Inibidores de Ciclo-Oxigenase/farmacologia , Neoplasias Esofágicas/genética , Neoplasias Esofágicas/patologia , Neoplasias Esofágicas/fisiopatologia , Regulação Enzimológica da Expressão Gênica/efeitos dos fármacos , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , Humanos , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Nus , Transplante de Neoplasias , Interferência de RNA , RNA Interferente Pequeno/genética , RNA Interferente Pequeno/metabolismo , TransfecçãoRESUMO
Erythropoietin (EPO) is the major means of treating anemia of chronic disease (ACD) through stimulating hematopoiesis, inhibiting hepcidin and decreasing proinflammatory factors. Recently, it has been found that monocytes are another source of hepcidin. EPO can reduce the hepcidin stimulated by IL-6 in monocytes, it is assumed that EPO can reduce hepcidin indirectly by reducing IL-6. However, the specific mechanism of EPO inhibiting the proinflammatory cytokines in monocytes is unclear now. This study was purposed to investigate the effect of EPO on monocyte proinflammatory factors and its molecular mechanism. IL-6 mRNA and TNF-α mRNA were detected by real time PCR, level of signaling molecule PARP-1 protein was detected by Western blot. THP-1 monocytes were stimulated by 1 µg/ml lipopolysaccharide (LPS) to observe the impact of EPO at different concentrations (0.5, 1, 2, 5, 10 U/ml) for different time (0, 3, 6, 12, 24 hours) on the expression of IL-6 mRNA, TNF-α mRNA and PARP-1 protein. 1 µg/ml or 5 µg/ml EPO receptor (EPOR) antibody and/or 3-aminobenzamide (3-AB, PARP-1 inhibitor) were added to observe the antagonistic effect on EPO and the impact on PARP-1. The results showed that LPS could stimulate the THP-1 cells. EPO could decrease the levels of IL-6 and TNF-α stimulated by LPS in a dose- and time-dependent manners. The most significant decrease in IL-6 mRNA expression was observed in 2 U/ml EPO for 6 hours. And down-regulation of TNF-α mRNA expression was pronounced at 10 U/ml EPO for 3 hours. IL-6 mRNA expression could be stimulated by LPS, PARP-1 protein was induced at the same time. EPO inhibited the expression of IL-6 mRNA, while PARP-1 protein also decreased. Down-regulation of IL-6 mRNA and PARP-1 protein level was pronounced at 2 U/ml EPO for 6 hours. 3AB is a direct inhibitor of PARP-1. Similar to 3AB, EPO receptor antibody could antagonize the decline of IL-6 induced by EPO. It is concluded that EPO can inhibit the expression of IL-6 and TNF-α in monocytes, and the inhibition of IL-6 expression may be associated with decrease of PARP level.
Assuntos
Anemia/metabolismo , Eritropoetina/farmacologia , Monócitos/efeitos dos fármacos , Monócitos/metabolismo , Linhagem Celular , Humanos , Interleucina-6/metabolismo , Poli(ADP-Ribose) Polimerase-1 , Poli(ADP-Ribose) Polimerases/metabolismo , Fator de Necrose Tumoral alfa/metabolismoRESUMO
OBJECTIVE: To investigate the in vitro effect of erythropoietin (EPO) on hepcidin of monocytes and its molecular mechanisms. METHODS: Hepcidin and signaling molecules including C/EBPalpha, Smad1/5/8, p-Smad1/5/8 and p-STAT3 were detected by real time PCR and Western blot. THP-1 monocytes were stimulated by interleukin-6 (IL-6) or lipopolysaccharide (LPS). EPO receptor (EPOR) antibody was added to observe its antagonistic effect on EPO and impact on the signaling proteins. RESULTS: EPO suppressed mRNA expression of THP-1 hepcidin of monocytes induced by 20 ng/ml IL-6 or 1 microg/ml LPS in both dose and time dependent manner. The most decrease of hepcidin expression was observed at 2 IU/ml EPO for 6 hours. EPO also down-regulated hepcidin protein induced by 20 ng/ml IL-6. At 2 IU/ml EPO for 6 hours hepcidin protein was down-regulated, as was C/EBPalpha, p-Smad1/5/8 and p-STAT3. Antibody to EPOR antagonized the down-regulation of EPO on hepcidin and signaling proteins. CONCLUSIONS: Monocytes hepcidin can be reduced by EPO when stimulated by IL-6 or LPS. The mechanism of which may be at least in part, via suppression of C/EBPalpha, p-Smad1/5/8 and p-STAT3 signaling.
Assuntos
Peptídeos Catiônicos Antimicrobianos/metabolismo , Eritropoetina/farmacologia , Monócitos/efeitos dos fármacos , Monócitos/metabolismo , Células Cultivadas , Hepcidinas , Humanos , Interleucina-6/farmacologia , Lipopolissacarídeos/farmacologia , Transdução de SinaisRESUMO
OBJECTIVE: To explore the effects of sodium selenite on the expressions of beta-catenin and cyclin D1 in colorectal cancer cells HCT 116 and SW480. METHODS: HCT 116 and SW480 cells were treated by 10 micromol/L sodium selenite at different time points. The expressions and transcription of beta-catenin and cyclin D1 were detected by Western blot analysis and reverse transcriptase polymerase chain reaction (RT-PCR), respectively. Meanwhile, the impact of MG132 (a proteasome inhibitor) pretreatment on the expressions of beta-catenin and cyclin D1 was observed through Western blot analysis. The interaction between beta-catenin and T cell factor 4 (TCF4) after selenite treatment was evaluated using co-immunoprecipitation assay. RESULTS: Sodium selenite inhibited the expression of beta-catenin and transcription of its target such as cyclin D1. MG132 pretreatment prevented the inhibition of beta-catenin signaling triggered by selenite in HCT 116 and SW480 cells. Furthermore, selenite treatment disrupted the interaction between beta-catenin and TCF4 in HCT 116 and SW480 cells. CONCLUSIONS: Sodium selenite can lower the expression levels of beta-catenin and its target cyclin D1, during which the proteasome-mediated degradative pathway may be involved. The decreased interaction between beta-catenin and TCF4 due to sodium selenite may be also involved in the regulation of beta-catenin signaling.
Assuntos
Neoplasias Colorretais/metabolismo , Ciclina D1/metabolismo , Selenito de Sódio/farmacologia , beta Catenina/metabolismo , Linhagem Celular Tumoral , Células HCT116 , HumanosRESUMO
We revealed in our previous research that sodium selenite induced obvious apoptosis of human leukemia NB4 cells, with reactive oxygen species (ROS), mitochondrial apoptosis pathway, and endoplasmic reticulum stress (ER stress) involved. In the present study, we revealed protein kinase Ca (PKCalpha) was dramatically downregulated in selenite-induced apoptosis, which was mediated by ROS. Besides, we confirmed PKCalpha played an antiapoptotic role through its effects on ERK1/2 and Akt, while its downregulation was attributed to caspase-3 and PP2Ac under the regulation of ROS. In summary, we speculated that in apoptosis of NB4 cells induced by selenite, PKCalpha functioned to counteract apoptosis, thus its downregulation seemed a mechanism aggravating apoptosis.
Assuntos
Antineoplásicos/farmacologia , Apoptose/efeitos dos fármacos , Proteína Quinase C-alfa/antagonistas & inibidores , Selenito de Sódio/farmacologia , Caspase 3/fisiologia , Linhagem Celular Tumoral , Regulação para Baixo , MAP Quinases Reguladas por Sinal Extracelular/metabolismo , Humanos , Leucemia Promielocítica Aguda/tratamento farmacológico , Leucemia Promielocítica Aguda/patologia , Proteína Fosfatase 2/fisiologia , Proteínas Proto-Oncogênicas c-akt/metabolismo , Espécies Reativas de OxigênioRESUMO
Cyclooxygenase-2 (COX-2) has been shown to be upregulated in a variety of tumors so that COX-2 may be a potential target in the treatment of cancer. In order to further explore the mechanism, we used RNA interference to study effects of the inhibition of COX-2 on esophageal squamous cell carcinoma (ESCC) lines. Western blot analysis demonstrated that COX-2 expression was significantly reduced in ESCC cells treated with the COX-2-specific siRNA. Furthermore, the COX-2 siRNA treatment inhibited cell proliferation and induced apoptosis in ESCC cells. In addition, the combination treatment of COX-2 siRNA and acidum acetil salicylicum (aspirin) has a synergistic effect. Therefore, this combination has potential as an anticancer therapy for the treatment of ESCC.
Assuntos
Apoptose/efeitos dos fármacos , Carcinoma de Células Escamosas/enzimologia , Proliferação de Células/efeitos dos fármacos , Inibidores de Ciclo-Oxigenase 2/farmacologia , Ciclo-Oxigenase 2/metabolismo , Neoplasias Esofágicas/enzimologia , Anti-Inflamatórios não Esteroides/farmacologia , Aspirina/farmacologia , Western Blotting , Carcinoma de Células Escamosas/patologia , Linhagem Celular Tumoral , Separação Celular , Ciclo-Oxigenase 2/efeitos dos fármacos , Neoplasias Esofágicas/patologia , Citometria de Fluxo , Humanos , RNA Interferente Pequeno , TransfecçãoRESUMO
Abnormalities and impaired functions of the olfactory system have been reported in Alzheimer's disease (AD), and these changes may appear much earlier than other clinical symptoms of AD. However, little is known about these abnormalities at the level of gene expression. In this study, we investigated alterations of expression of 22,012 genes in the olfactory bulbs of a rat model of AD by using a microarray approach. The rat model was produced by intracerebroventricular injection of amyloid-beta(25-35), which demonstrated pathological changes in olfactory bulbs and memory impairment in the Morris water maze test. We found that expression of 811 genes among the 22,012 genes was altered by more than 1.5-fold in the amyloid-beta-injected rats as compared with control injected rats. We analyzed the distribution of the 811 altered genes according to the Affymetrix criteria and found that the majority of these genes were related to cellular processes, binding, and enzyme activities. The alterations of three of these genes, i.e., calcineurin, olfactory receptor, and protein kinase C, were also confirmed by RT-PCR and Western blots. These studies provide new insight into the abnormalities of the olfactory system in AD and might help to further the understanding of the molecular mechanisms of AD.
Assuntos
Doença de Alzheimer/genética , Doença de Alzheimer/fisiopatologia , Perfilação da Expressão Gênica/métodos , Bulbo Olfatório/fisiopatologia , Doença de Alzheimer/metabolismo , Peptídeos beta-Amiloides/metabolismo , Animais , Western Blotting , Encéfalo/metabolismo , Encéfalo/fisiopatologia , Primers do DNA/genética , Taxa de Filtração Glomerular/fisiologia , Masculino , Análise de Sequência com Séries de Oligonucleotídeos , Distribuição Aleatória , Ratos , Ratos Sprague-Dawley , Reação em Cadeia da Polimerase Via Transcriptase ReversaRESUMO
We investigate the correlation between the glycosylation modified prion proteins and apoptosis. The wild-type PRNP gene and four PRNP gene glycosylated mutants were transiently expressed in HeLa cells. The effect of apoptosis induced by PrP mutants was confirmed by MTT assay, Hochest staining, Annexin-V staining and PI staining. ROS test detected ROS generation within the cells. The mitochondrial membrane potential was analyzed by the flow cytometry. The expression levels of Bcl-xL, Bax, cleaved Caspase-9 proteins were analyzed by Western Blot. The results indicated that the expressed non-glycosylated PrP in HeLa cells obviously induced apoptosis, inhibited the growth of cells and reduced the mitochondrial membrane potential, and more ROS generation and low levels of the apoptosis-related proteins Bcl-xL, the activated the cleaved Caspase-9 proteins were found. The apoptosis induced by non-glycosylated PrP demonstrates that its underlying mechanism correlates with the mitochondria-mediated signal transduction pathway.
Assuntos
Apoptose , Células HeLa , Príons/metabolismo , Príons/fisiologia , Apoptose/fisiologia , Caspase 9/metabolismo , Proliferação de Células , Sobrevivência Celular , Glicosilação , Humanos , Proteínas Mutantes/metabolismo , Proteínas Mutantes/fisiologia , Príons/química , Príons/genética , Processamento de Proteína Pós-Traducional/fisiologia , Espécies Reativas de Oxigênio/metabolismo , Distribuição Tecidual , Transfecção , Proteína bcl-X/metabolismoRESUMO
OBJECTIVE: To explore the mechanism and significance of cytochrome c oxidase subunit IV (COX IV) downregulation during apoptosis of NB4 cells induced by sodium selenite. METHODS: NB4 cells were treated with 20 micromol/L sodium selenite at different time points. COX IV protein and mRNA were detected by Western blot and RT-PCR, respectively. NB4 cells were pretreated with reactive oxygen species (ROS) scavenger before selenite exposure, and then COX IV protein expression and caspase-3 activation were detected by Western blot. NB4 cells were pretreated with caspase-3 inhibitor before selenite exposure, and then COX IV protein expression was detected by Western blot. NB4 cells were transiently transfected with vectors to interfere with the expression of COX IV, and then the apoptosis induced by selenite was analyzed by flow cytometry. RESULTS: Sodium selenite induced evident downregulation of COX IV protein in NB4 cells, while its mRNA level was almost unchanged. ROS scavenger completely reversed selenite-induced COX IV downregulation and caspase-3 activation. Caspase-3 inhibitor partially reversed selenite-induced COX IV downregulation. Interference with COX IV expression dramatically enhanced selenite-induced apoptosis of NB4 cells. CONCLUSIONS: COX IV is remarkably downregulated during selenite-induced apoptosis of NB4 cells. ROS mediates COX IV downregulation and caspase-3 activation, while caspase-3 is partially involved in COX IV downregulation. COX IV interference markedly increases the sensitivity of NB4 cells to selenite-induced apoptosis.
Assuntos
Apoptose/efeitos dos fármacos , Complexo IV da Cadeia de Transporte de Elétrons/metabolismo , Selenito de Sódio/farmacologia , Caspase 3/metabolismo , Linhagem Celular Tumoral , Regulação para Baixo/efeitos dos fármacos , Complexo IV da Cadeia de Transporte de Elétrons/genética , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , Humanos , RNA Mensageiro/genética , Espécies Reativas de Oxigênio/metabolismoRESUMO
Although the function of cellular prion protein (PrPc) and the pathogenesis of prion diseases have been widely described, the mechanisms are not fully clarified. In this study, increases of the portion of non-glycosylated prion protein deposited in the hamster brains infected with scrapie strain 263K were described. To elucidate the pathological role of glycosylation profile of PrP, wild type human PrP (HuPrP) and two genetic engineering generated non-glycosylated PrP mutants (N181Q/N197Q and T183A/T199A) were transiently expressed in human astrocytoma cell line SF126. The results revealed that expressions of non-glycosylated PrP induced significantly more apoptosis cells than that of wild type PrP. It illustrated that Bcl-2 proteins might be involved in the apoptosis pathway of non-glycosylated PrPs. Our data highlights that removal of glycosylation of prion protein provokes cells apoptosis.
Assuntos
Apoptose/fisiologia , Encéfalo/metabolismo , Príons/metabolismo , Animais , Apoptose/genética , Western Blotting , Encéfalo/patologia , Linhagem Celular Tumoral , Cricetinae , Citometria de Fluxo , Imunofluorescência , Glicosilação , Humanos , Potencial da Membrana Mitocondrial , Mutação , Nucleotídeos/metabolismo , Plasmídeos/genética , Proteínas PrPSc/metabolismo , Príons/genética , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Transfecção , Proteína X Associada a bcl-2/metabolismoRESUMO
OBJECTIVE: To investigate the role of reactive oxygen species (ROS) and ROS-caused mitochondrial transmembrane potential loss in sodium selenite-induced apoptosis in NB4 cells. METHODS: ROS production was measured by ROS-specific probe DCFH-DA. Sodium selenite mitochondrial transmembrane potential loss was evaluated by flow cytometry with Rh123 staining. Protein levels of cytochrome C, Bid, Bcl-xl, and Bax were measured by Western blot using protein-specific antibodies. NB4 cells were pre-incubated by MnTmPy or BSO before selenite treatment to further confirm the effects of ROS on NB4 cells. RESULTS: 20 micromol/L sodium selenite induced ROS production and mitochondrial transmembrane potential loss in NB4 cells time-dependently. Cytochrome C accumulated in cytoplasm after selenite treatment. Sodium selenite also downregulated Bcl-xl and activated Bax and Bid at protein level. Pretreatment with antioxidant MnTmPy almost fully abrogated the proapoptotic effect of sodium selenite prevented the cleavage of Bid protein and in turn the mitochondrail transmembrane potential loss. On the contrary, pretreatment with BSO intensified the mitochondrail transmembrane potential loss induced by sodium selenite. CONCLUSIONS: Sodium selenite may induce apoptosis by inducing ROS production in NB4 cells, which leads to the downregulation of Bcl-xl, upregulation of Bax, and cleavage and activation of Bid. Bax and tBid then agregate on mitochondrial membrane, which in turn causes a decrease of mitochondrial transmembrane potential and release of cytochrome C into cytoplasm.
Assuntos
Apoptose , Potencial da Membrana Mitocondrial/efeitos dos fármacos , Espécies Reativas de Oxigênio/metabolismo , Selenito de Sódio/farmacologia , Proteína Agonista de Morte Celular de Domínio Interatuante com BH3/biossíntese , Linhagem Celular Tumoral , Citocromos c/metabolismo , Humanos , Proteína X Associada a bcl-2/biossíntese , Proteína bcl-X/biossínteseRESUMO
To study the biological function of the N-glycosylation modification of prion proteins (PrP), various eukaryotic expression vectors for the mutants with N-glycosylation modification of human PrP had been constructed and expressed. With site-direct mutation technique, human PRNP gene was mutated and the obtained mutants were subcloned into eukaryotic expressing plasmid pcDNA3.1 and transiently expressed in Hela cervical adenocarcinoma cell. The expression products of the mutated PrP were identified with Western blotting assay and the PNGase digestion assay. Several mutants with specific glycosylation modification were identified from the expressed products by Western blot, including two mutants with one glycosylation site mutated and one without any mutation at glycosylation sites. The expressed products were digested with PNGase F. The wild type proteins and those with one of glycosylation sites mutated were digested, resulting in their molecular weights reduced, while the molecular weights of products with mutations at both glycosylation sites were not changed. The mutant of wild type human PRNP gene at N-glycosylation modification sites and six modified mutants with mono- or non-N-glycosylation had been obtained successfully in the study. Moreover, the modified PrP with mono- and non-N-glycosylation were able to be expressed transitantly in Hela cells, which could be a useful means for studying prions.
Assuntos
Proteínas Mutantes/genética , Príons/biossíntese , Príons/genética , Escherichia coli/genética , Escherichia coli/metabolismo , Produtos Finais de Glicação Avançada/biossíntese , Produtos Finais de Glicação Avançada/genética , Glicosilação , Células HeLa , Humanos , Mutagênese Sítio-Dirigida , Proteínas Mutantes/biossíntese , TransfecçãoRESUMO
Selenium at a low concentration has a chemopreventive role against cancer, while at a high concentration, it exerts a direct antitumor effect. However, the mechanisms remain elusive. In this article, we discovered that Na(2)SeO(3) at 20 micromol/l concentration could significantly inhibit the proliferation of NB4 cells, affect the cell cycle distribution of cell population, and induce cellular changes characteristic of apoptotic cells, while this same compound at 2 micromol/l concentration had no such effects. The mechanisms underlying these overt differences caused by treatment of different concentrations of selenium were further investigated. cDNA microarray analysis showed that after treatment by 20 micromol/l Na(2)SeO(3), 34 genes were changed in expression, while treatment by 2 micromol/l Na(2)SeO(3) resulted in the changes of 29 genes. Nine genes were regulated in both groups, among which three showed opposite changes caused by 2 and 20 micromol/l Na(2)SeO(3). The majority of regulated genes did not coincide between the two experiment groups. In conclusion, 2 and 20 micromol/l Na(2)SeO(3) could have different effects on NB4 cells, and some genes might be involved in the underlying mechanisms. Our findings could provide basis for further uncovering the molecular mechanisms of the chemopreventive and antitumor effects of selenium and, in turn, for probing the rationality of treating leukemia with selenium.
Assuntos
Apoptose/efeitos dos fármacos , Ciclo Celular/efeitos dos fármacos , Perfilação da Expressão Gênica , Selenito de Sódio/farmacologia , Divisão Celular/efeitos dos fármacos , Linhagem Celular Tumoral , Fragmentação do DNA , Primers do DNA , Relação Dose-Resposta a Droga , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , Humanos , Leucemia Promielocítica Aguda , Reação em Cadeia da Polimerase Via Transcriptase ReversaRESUMO
Two relatively recent discoveries stand behind our current effort to investigate the effects of the chemopreventive agent, selenium, on the proliferation and survival of NB4 cells. The first is that certain selenium compounds such as sodium selenite have pro-oxidant ability to catalyze the oxidation of thiols and simultaneously generate superoxide. The second lies in the exquisite susceptibility of NB4 cells to arsenic trioxide-induced, reactive oxygen species (ROS)-mediated apoptosis due to less efficiency of the cellular defense system. In this study, we demonstrated that sodium selenite could induce apoptosis in NB4 cells via the classic mitochondrial pathway involving caspase-3 activation and Bcl-2 cleavage. An increase in the basal cellular glutathione (GSH) content rendered NB4 cells resistant to arsenic trioxide, but could sensitize NB4 cells to sodium selenite. Moreover, combined treatment of NB4 cells with all- trans retinoic acid (ATRA) at low concentration and sodium selenite exhibited a synergistic effect on apoptosis induction. Together, our results suggest that selenite is a promising candidate for treatment of acute promyelocytic leukemia (APL) and the mechanism underlying its anticancer effects warrants further investigation.
Assuntos
Antineoplásicos/farmacologia , Apoptose/efeitos dos fármacos , Caspases/metabolismo , Leucemia Promielocítica Aguda/tratamento farmacológico , Selenito de Sódio/farmacologia , Tretinoína/farmacologia , Trióxido de Arsênio , Arsenicais/farmacologia , Caspase 3 , Linhagem Celular Tumoral , Relação Dose-Resposta a Droga , Resistencia a Medicamentos Antineoplásicos , Sinergismo Farmacológico , Ativação Enzimática/efeitos dos fármacos , Humanos , Leucemia Promielocítica Aguda/metabolismo , Leucemia Promielocítica Aguda/patologia , Mitocôndrias/metabolismo , Oxirredução/efeitos dos fármacos , Óxidos/farmacologia , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Transdução de Sinais/efeitos dos fármacos , Compostos de Sulfidrila/metabolismoRESUMO
OBJECTIVE: To understand the infectious characteristics of a hamster-adapted scrapie strain 263K with five different routes of infection including intracerebral (i.c.), intraperitoneal (i.p.), intragastrical (i.g.), intracardiac and intramuscular (i.m.) approaches. METHODS: Hamsters were infected with crude- or fine-prepared brain extracts. The neuropathological changes, PrP(Sc) deposits, and patterns of PK-resistant PrP were analyzed by HE stain, immnunohistochemistry (IHC) assay and Western blot. Reactive gliosis and neuron loss were evaluated by glial fibrillary acidic protein (GFAP) and neuron specific enolase (NSE) specific IHC. RESULTS: The animals inoculated in i.m. and i.p. ways with crude PrP(Sc) extracts showed clinical signs at the average incubation of 69.2 +/- 2.8 and 65.5 +/- 3.9 days. Inoculation in i.c. and intracardiac ways with fine PrP(Sc) extracts (0.00035 g) caused similar, but relative long incubation of around 90 days. Only one out of eight hamsters challenged in i.g. way with low dosage (0.01 g) became ill after a much longer incubation (185 d), while all animals (4/4) with high dosage (0.04 g) developed clinical signs 105 days postinfection. The most remarkable spongiform degeneration and PrP(Sc) deposits were found in brain stem among the five challenge groups generally. The number of GFAP-positive astrocytes increased distinctly in brain stems in all infection groups, while the number of NSE-positive cells decreased significantly in cerebrum, except i.c. group. The patterns of PK-resistant PrP in brains were basically identical among the five infection routes. CONCLUSION: Typical TSE could be induced in hamsters by inoculating strain 263K in the five infection ways. The incubation periods in bioassays depend on infective dosage, administrating pathway and preparation of PrP(Sc). The neuropathological changes and PrP(Sc) deposits seem to be related with regions and inoculating pathways.
Assuntos
Encéfalo/patologia , Príons , Scrapie/patologia , Administração Oral , Animais , Western Blotting , Encéfalo/metabolismo , Cricetinae , Gliose/metabolismo , Gliose/patologia , Imuno-Histoquímica , Injeções Intramusculares , Injeções Intraperitoneais , Injeções Intraventriculares , Neurônios/enzimologia , Neurônios/patologia , Fosfopiruvato Hidratase/metabolismo , Príons/administração & dosagem , Príons/metabolismo , Príons/patogenicidade , Scrapie/metabolismoRESUMO
OBJECTIVE: To explore the regulational effect of oxidized low-density lipoprotein (Ox-LDL) on expression of type A scavenger receptor (SR-A) in human mesangial cells (HMC). METHODS: HMC line (HMCL) with high expression of SR-A (HMCL-SRA) was established after stable transfection of expressive vector with cDNA encoding SR-A. Uptake of Ox-LDL by HMCL was evaluated using Oil Red "O" staining. SR-A mRNA expression was examined using reverse transcription-polymerase chain reaction (RT-PCR). RESULTS: More uptake of Ox-LDL was observed in the HMCL-SRA than that in the untransfected HMCL. Ox-LDL could induce SR-A mRNA expression in HMC in a dose-dependent manner, and reached a peak level after 24 h of stimulation. After 24 h of stimulation with Ox-LDL at the dose of 10, 50 and 100 micrograms/ml, SR-A mRNA expression was up-regulated to 1.35, 1.83 and 2.30-fold of controls, respectively. However, LDL had no effect on the expression of SR-A. CONCLUSIONS: It suggests that SR-A be a major binding receptor to uptake Ox-LDL in HMC. Ox-LDL may promote the progression of chronic renal diseases through up-regulation of SR-A.
Assuntos
Mesângio Glomerular/metabolismo , Lipoproteínas LDL/farmacologia , Receptores Imunológicos/biossíntese , Células Cultivadas , DNA Complementar , Mesângio Glomerular/citologia , Humanos , RNA Mensageiro/biossíntese , RNA Mensageiro/genética , Receptores Imunológicos/genética , Receptores Depuradores , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Receptores Depuradores Classe A , Transfecção , Regulação para CimaRESUMO
The mechanisms involved in the anti-carcinogenic activity of selenium remained to be elucidated. In the present study, we examined sodium selenite induced apoptosis and oxidative stress in human acute promyelocytic leukemia cell lines (NB4). Cell growth and viability were assessed by trypan blue exclusion and cell counting; apoptosis by DNA electrophoresis and analysis of intracellular DNA contents; reactive oxygen species and reduced glutathione in the cell were measured by lucigenin dependent chemoluminescent (CL) test and spectrophotometer; mitochondrial transmembrane potential was measured by flow cytometry. Sodium selenite could inhibit the growth and induce apoptosis of NB4 cells. Sodium selenite could increase the production of reactive oxygen species (ROS) in NB4 cells and decrease the level of intracellular reduced glutathione, but caused no change in the activity of antioxidant enzymes, superoxide dismutase (SOD), glutathione peroxidase (GPx). Sodium selenite enhanced the collapse of mitochondrial transmembrane potential (MTP), in parallel with the production of ROS. Finally antioxidant N-acetylcysteine (NAC) could inhibit the ROS production, MTP collapse and apoptosis in NB4 cells. Our results suggested that sodium selenite could induce apoptosis of NB4 cells through mitochondrial change mediated by production of reactive oxygen species within the cells.
Assuntos
Apoptose , Leucemia Promielocítica Aguda/tratamento farmacológico , Leucemia Promielocítica Aguda/patologia , Mitocôndrias/fisiologia , Estresse Oxidativo , Selenito de Sódio/farmacologia , Acetilcisteína/farmacologia , Acridinas/farmacologia , Divisão Celular , Sobrevivência Celular , Fragmentação do DNA , Eletroforese em Gel de Ágar , Citometria de Fluxo , Fase G1 , Glutationa/metabolismo , Glutationa Peroxidase/metabolismo , Humanos , Medições Luminescentes , Potenciais da Membrana , Espécies Reativas de Oxigênio , Superóxido Dismutase/metabolismo , Fatores de Tempo , Células Tumorais CultivadasRESUMO
OBJECTIVE: To study the feasibility of diagnosing paroxysmal nocturnal hemoglobinuria (PNH) with toxin HEC, the abbreviation of hemolytic, entreotoxigenicity and cytotoxity secreted by Aeromonas hydrophila J-1. METHODS: The crude toxin HEC was extracted from the culture medium of Aeromonas hydrophila J-1 by precipitating with saturated (NH(4))(2)SO(4) and then purified through DEAE52. Purified toxin HEC is different from Aerolysin in molecular weight and necessity of activation. Crude toxin is prepared possessed same effect as purified ones. This crude toxin was used to act on red blood cells (RBCs) from patients with PNH, non-PNH anemia, and normal persons. Absorbance at 630 nm was measured to quantitate the extent of hemolysis. Toxin HEC treated and untreated RBCs were both stained with anti-CD59 monoclonal antibody and FITC labeled goat-anti-mouse IgG. The percentage of CD59(+) cells was detected by flow cytometry (FCM). RESULTS: After toxin HEC treatment, RBCs from PNH patients showed resistance to the toxin hemolysis, which was negatively related to the percentage of CD59(+) cells, while RBCs from normal persons and non-PNH anemic patient were nearly totally lysed. CONCLUSION: Detection of RBCs resistance to toxin HEC can be used for the diagnosis of PNH.
Assuntos
Aeromonas hydrophila/química , Toxinas Bacterianas/farmacologia , Membrana Eritrocítica/efeitos dos fármacos , Glicosilfosfatidilinositóis/deficiência , Hemoglobinúria Paroxística/diagnóstico , Hemólise/efeitos dos fármacos , Anemia/sangue , Toxinas Bacterianas/isolamento & purificação , Antígenos CD59/análise , Centrifugação , Precipitação Química , Cromatografia DEAE-Celulose , Cromatografia por Troca Iônica , Dextranos , Relação Dose-Resposta a Droga , Resistência a Medicamentos , Membrana Eritrocítica/química , Hemoglobinúria Paroxística/sangue , Humanos , Fatores de TempoRESUMO
The purpose of the study is to establish a colorimetric method of HEC toxin hemolysis test for diagnosis of paroxysmal nocturnal hemoglobinuria (PNH). RBCs from normal persons and patients with PNH and non-PNH anemia were treated with HEC toxin secreted by Aeromonas hydrophila J-1 strain and the absorbance at 630 nm was measured to quantitate the extent of hemolysis. The results demonstrated that the RBCs from PNH patients showed resistance to the toxin hemolysis, which was in accord with the percentages of CD59(-) cells, while the RBCs from normal persons and non-PNH anemic patients were nearly totally lysed. It is concluded that the method can be considered as a simple, specific and reliable method for the diagnosis of PNH.
Assuntos
Toxinas Bacterianas/toxicidade , Hemoglobinúria Paroxística/diagnóstico , Hemólise , Colorimetria , Citometria de Fluxo , HumanosRESUMO
To study the relationship of Glycosyl phosphatidylinositol anchored proteins (GIP-Pr) and apoptosis of paroxysmal nocturnal hemoglobinuria (PNH) cells, we isolated peripheral granulocytes from 10 patients with PNH and 10 normal controls and measured apoptosis induced by serum starvation. The FCM analysis of phosphotidylserine (ps) externalization in granulocytes was determined using Annexin-V-FLUOS labeling. After the cells were induced for apoptosis in serum-free medium for 20 hours, the percentage of externalization was 78.6% in normal control cells but 39.5% in PNH cells. The results of FCM analysis of PI stained granulocytes showed that the PI positive rate was 51.5% in control cells and 30.2% in PNH cells. The gel electrophoresis analysis of DNA fragmentation all indicate that PNH granulocytes were relatively resistant to apoptosis as compared with normal controls. This resistance to apoptosis might not be related to the percentage of CD59 deficient granulocytes.