Improved production of adipic acid from a high loading of corn stover via an efficient and mild combination pretreatment.

Adipic acid is one kind of important organic dibasic acid, which has crucial role in manufacturing plastics, lubricants, resins, fibers, etc. Using lignocellulose as feedstock for producing adipic acid can reduce production cost and improve bioresource utilization. After pretreated in the mixture of 7 wt% NaOH and 8 wt% ChCl-PEG10000 at 25 °C for 10 min, the surface of corn stover became loose and rough. The specific surface area was increased after the removal of lignin. A high loading of pretreated corn stover was enzymatically hydrolyzed by cellulase (20 FPU/g substrate) and xylanase (15 U/g substrate), and the yield of reducing sugars was as high as 75%. Biomass-hydrolysates obtained by enzymatic hydrolysis were efficiently fermented to produce adipic acid, and the yield was 0.48 g adipic acid per g reducing sugar. A sustainable approach for manufacturing adipic acid from lignocellulose via a room temperature pretreatment has great potential in future.

Significant Enhancement of 5-Hydroxymethylfural Productivity from D-Fructose with SG(SiO2) in Betaine:Glycerol-Water for Efficient Synthesis of Biobased 5-(Hydroxymethyl)furfurylamine.

5-Hydroxymethyl-2-furfurylamine (5-HMFA) as an important 5-HMF derivative has been widely utilized in the manufacture of diuretics, antihypertensive drugs, preservatives and curing agents. In this work, an efficient chemoenzymatic route was constructed for producing 5-(hydroxymethyl)furfurylamine (5-HMFA) from biobased D-fructose in deep eutectic solvent Betaine:Glycerol-water. The introduction of Betaine:Glycerol could greatly promote the dehydration of D-fructose to 5-HMF and inhibit the secondary decomposition reactions of 5-HMF, compared with a single aqueous phase. D-Fructose (200 mM) could be catalyzed to 5-HMF (183.4 mM) at 91.7% yield by SG(SiO2) (3 wt%) after 90 min in Betaine:Glycerol (20 wt%), and at 150 °C. E. coli AT exhibited excellent bio-transamination activity to aminate 5-HMF into 5-HMFA at 35 °C and pH 7.5. After 24 h, D-fructose-derived 5-HMF (165.4 mM) was converted to 5-HMFA (155.7 mM) in 94.1% yield with D-Ala (D-Ala-to-5-HMF molar ratio 15:1) in Betaine:Glycerol (20 wt%) without removal of SG(SiO2), achieving a productivity of 0.61 g 5-HMFA/(g substrate D-fructose). Chemoenzymatic valorization of D-fructose with SG(SiO2) and E. coli AT was established for sustainable production of 5-HMFA, which has potential application.

Biodegradation of alkali lignin by a newly isolated Rhodococcus pyridinivorans CCZU-B16.

Based on the Prussian blue spectrophotometric method, one high-throughput screening strategy for screening lignin-degrading microorganisms was built on 24-well plate at room temperature. One high activity of alkali lignin-degrading strain Rhodococcus pyridinivorans CCZU-B16 was isolated from soil. After the optimization of biodegradation, 30.2% of alkali lignin (4 g/L) was degraded under the nitrogen-limited condition (30/1 of C/N ratio; g/g) at 30 °C for 72 h. It was found that syringyl (S) units and guaiacyl (G) in lignin decreased after biodegradation. Moreover, the accumulated lipid in cells had a fatty acid profile rich in C16 and C18 with four major constituent fatty acids including palmitic acid (C16:0; 22.4%), palmitoleic acid (C16:1; 21.1%), stearic acid (C18:0; 16.2%), and oleic acid (C18:1; 23.1%). In conclusion, Rhodococcus pyridinivorans CCZU-B16 showed high potential application in future.