Canopy height, rather than neighborhood effects, shapes leaf herbivory in a tropical rainforest.

Factors shaping the interspecific variations in herbivory have puzzled ecologists for decades and several hypotheses have been proposed to explain interspecific variation in leaf herbivory. In a tropical rainforest in Yunnan Province, China, we collected 6732 leaves from 129 species with canopy heights ranging from 1.6 to 65.0 m above the ground. We tested the role of canopy height, the diversity, composition and structural heterogeneity of neighbors and leaf traits in shaping the interspecific variations in herbivory. Results show that leaf herbivory decreased with canopy height and specific leaf area (SLA) and increased with leaf size. However, neighboring species' diversity, composition, and structural heterogeneity showed no association with herbivory. Therefore, neither the visual apparency effect nor the associational resistance effect was detected in this hyperdiverse tropical rainforest. These findings highlight the importance of vertical structure in shaping herbivory patterns in natural communities.

Soil microplastic pollution under different land uses in tropics, southwestern China.

Terrestrial ecosystems encounter emerging risks of microplastic (MP) pollution. However, the distribution characteristics of soil MPs across different land uses in tropical areas have remain largely unknown. We sampled soils from two natural ecosystems (primary and secondary forests) and two artificial ecosystems (rubber and banana plantations) in tropical region of southwestern China. We aimed to evaluate the overall characteristics of soil MPs and analyze the distribution and source of MPs in different soil layers and land uses. We found that the dominant size of soil MPs were <1 mm and the major shapes were fragments and fibres, with colours blue, yellow, and green-blue. Most MPs were polyethylene (PE, 59.6%), rayon (RY, 12.0%), and polypropylene (PP, 10.9%). In artificial ecosystems, the abundance of MPs in the top soil (0-10 cm) was approximately 2.5 times that of in deep soil (10-20 cm), whereas it was only 50% in the natural ecosystems. The abundance of MPs in banana plantations reached as high as 10975.0 ± 261.0 particles kg-1 (p kg-1), which was about 10 times that of in rubber plantations (1112.5 ± 151.6 p kg-1) and 18 times of those in secondary and primary forests (612.5 ± 119.2 p kg-1 and 637.5 ± 181.6 p kg-1). Anthropogenic and atmospheric transport may be the major sources of soil MPs for artificial ecosystems to natural ecosystems, respectively. Our results revealed the widespread presence of soil MPs in tropical areas, from artificial ecosystems to natural ecosystems, in both the top and deep soil layers. MP pollution in artificial ecosystems is considerably serious than that in natural ecosystems. Our study provides important support for further research on ecosystem risks pertaining to MPs in the context of land use changes, and promotes the development of effective measures and policies to control MP pollution in tropical areas.

[Scaling laws of altitudinal pattern of soil fauna diversity in Dongling Mountain, Beijing, China]. / åŒ—äº¬ä¸œçµå±±åœŸå£¤åŠ¨ç‰©å¤šæ ·æ€§æµ·æ‹”æ ¼å±€çš„å°ºåº¦æŽ¨ç»Žè§„å¾‹.

Identifying the complexity of diversity pattern of various taxa within a community is a challenge for ecologist. Scaling law is one of the suitable ways to detecting the complex ecological structure. In this study, we explored the scaling laws of soil fauna diversity pattern along an altitudinal gradient by multifractal analysis, and compared the difference of multifractal spectra between the litter and the soil layers. Consistent with results from plant communities in previous studies, there was power law scaling law for soil fauna diversity, i.e., richness, the exponential of Shannon's Diversity Index, and the inverse Simpson's Diversity Index. Moreover, power law scaling law also existed for the richness changes of different relative abundance species in both litter and soil layers. Although multifractal characteristics existed for both litter layer and soil layer of soil fauna diversity, the fractal structure of the diversity in the litter layer was more even than that in the soil layer, and the scaling properties of dominant and rare species showed different patterns in multifractal spectra between litter layer and soil layer. In conclusion, there were power law scaling laws for soil fauna diversity which had high richness and abundance along the altitudinal gradient, which would help us uncovering the spatial distribution mechanism of belowground biodiversity.

Impacts of Urbanization Undermine Nestedness of the Plant-Arbuscular Mycorrhizal Fungal Network.

Cities are prone to ecological problems, yet the impacts of rapid global urbanization on the feedback between above- and belowground subsystems remain largely unknown. We sampled the roots of 8 common herbaceous plants within the Fifth Ring (urban areas) and in Jiufeng National Forest Park (rural areas) in Beijing (China) to assess the impacts of urbanization on the network of plant-arbuscular mycorrhizal (AM) fungal associations. Using Illumina MiSeq sequencing, 81 AM fungal OTUs were identified in 78 herb root samples. The Shannon, Simpson, and Pielou indices of root AM fungi in urban areas were significantly higher than those in rural areas. In this study, a significantly nested mycorrhizal association network was observed in rural areas (NODF = 64.68), whereas a non-nested pattern was observed in urban areas (NODF = 55.50). The competition index C-score (0.0769) of AM fungi in urban areas was slightly lower than that in rural areas (0.1431), and the species specialization (d') of 8 host plants and fungal dissimilarity among 8 host plants in urban areas were significantly lower than those in rural areas. Convergent associations among hosts may be an important factor influencing this non-nested pattern of the plant-AM fungi network in urban areas. Generalists, rather than specialists, were enhanced during the establishment of mycorrhizal associations in urban areas. Our results suggest that reduced selectivity of host plants, and generalist promotion and specialist reduction of AM fungi during urbanization may contribute to the non-nested network of plant-AM fungal associations.

Opposite latitudinal patterns for bird and arthropod predation revealed in experiments with differently colored artificial prey.

The strength of biotic interactions is generally thought to increase toward the equator, but support for this hypothesis is contradictory. We explored whether predator attacks on artificial prey of eight different colors vary among climates and whether this variation affects the detection of latitudinal patterns in predation. Bird attack rates negatively correlated with model luminance in cold and temperate environments, but not in tropical environments. Bird predation on black and on white (extremes in luminance) models demonstrated different latitudinal patterns, presumably due to differences in prey conspicuousness between habitats with different light regimes. When attacks on models of all colors were combined, arthropod predation decreased, whereas bird predation increased with increasing latitude. We conclude that selection for prey coloration may vary geographically and according to predator identity, and that the importance of different predators may show contrasting patterns, thus weakening the overall latitudinal trend in top-down control of herbivorous insects.

Environmental correlates underlying elevational richness, abundance, and biomass patterns of multi-feeding guilds in litter invertebrates across the treeline.

Elevational richness patterns and underlying environmental correlates have contributed greatly to a range of general theories of biodiversity. However, the mechanisms underlying elevational abundance and biomass patterns across several trophic levels in belowground food webs remain largely unknown. In this study, we aimed to disentangle the relationships between the elevational patterns of different trophic levels of litter invertebrates and their underlying environmental correlates for two contrasting ecosystems separated by the treeline. We sampled 119 plots from 1020 to 1770 asl in forest and 21 plots from 1790 to 2280 asl in meadow on Dongling Mountain, northwest of Beijing, China. Four functional guilds were divided based on feeding regime: omnivores, herbivores, predators, and detritivores. We used eigenvector-based spatial filters to account for spatial autocorrelation and multi-model selection to determine the best environmental correlates for the community attributes of the different feeding guilds. The results showed that the richness, abundance and biomass of omnivores declined with increasing elevation in the meadow, whereas there was a hump-shaped richness pattern for detritivores. The richness and abundance of different feeding guilds were positively correlated in the forest, while not in the meadow. In the forest, the variances of richness in omnivores, predators, and detritivores were mostly correlated with litter thickness, with omnivores being best explained by mean annual temperature in the meadow. In conclusion, hump-shaped elevational richness, abundance and biomass patterns driven by the forest gradient below the treeline existed in all feeding guilds of litter invertebrates. Climate replaced productivity as the primary factor that drove the richness patterns of omnivores above the treeline, whereas heterogeneity replaced climate for herbivores. Our results highlight that the correlated elevational richness, abundance, and biomass patterns of feeding guilds are ecosystem-dependent and that the underlying environmental correlates shifted at the treeline for most feeding guilds.

Contrasting elevational diversity patterns for soil bacteria between two ecosystems divided by the treeline.

Above- and below-ground organisms are closely linked, but how elevational distribution pattern of soil microbes shifting across the treeline still remains unknown. Sampling of 140 plots with transect, we herein investigated soil bacterial distribution pattern from a temperate forest up to a subalpine meadow along an elevational gradient using Illumina sequencing. Our results revealed distinct elevational patterns of bacterial diversity above and below the treeline in responding to changes in soil conditions: a hollow elevational pattern in the forest (correlated with soil temperature, pH, and C:N ratio) and a significantly decreasing pattern in the meadow (correlated with soil pH, and available phosphorus). The bacterial community structure was also distinct between the forest and meadow, relating to soil pH in the forest and soil temperature in the meadow. Soil bacteria did not follow the distribution pattern of herb diversity, but bacterial community structure could be predicted by herb community composition. These results suggest that plant communities have an important influence on soil characteristics, and thus change the elevational distribution of soil bacteria. Our findings are useful for future assessments of climate change impacts on microbial community.