RESUMO
Wastewater-based epidemiology (WBE) is an emerging discipline, which has been applied to drug abuse tracking and infectious disease pathogen surveillance. During the COVID-19 epidemic, WBE has been applied to monitor the epidemic trend and SARS-CoV-2 variants etc. In order to detect hidden COVID-19 cases and prevent transmission in the community, wastewater surveillance system for monitoring SARS-CoV-2 RNA was developed in Shenzhen. The sewage sampling sites were set up in key places such as the port areas, urban villages and residential communities of Futian, Nanshan, Luohu and Yantian districts. From July 26 to November 30, 2022, a total of 369 sewage sampling sites were set up, covering 1.93 million people. Continuous sampling was carried out for 3 hours in the peak period of water use every day. Sewage virus enrichment and SARS-CoV-2 nucleic acid detection were carried out by polyethylene glycol precipitation method and RT-qPCR, and a positive water sample disposal process was molded. This article aims to introduce the case of source tracing of COVID-19 infected patients based on urban sewage in Shenzhen. The sewage monitoring of Honghu water treatment plant in Luohu District played an early warning role, and the source of infection was traced. In the disposal of positive water samples in Futian South Road, Futian District, the important experience of monitoring point layout was obtained. In the sewage monitoring of Nanshan village, Nanshan District, the existence of occult infection was revealed. Sharing the experience of tracing the source of COVID-19 patients to avoid the spread of COVID-19 in the community based on wastewater surveillance of SARS-CoV-2 RNA in Shenzhen, and summarizing the advantages and application prospects of sewage surveillance can provide new ideas for monitoring emerging or re-emerging pathogens that are known to exhibit gastrointestinal excretion in the future.
Assuntos
COVID-19 , SARS-CoV-2 , Humanos , Vigilância Epidemiológica Baseada em Águas Residuárias , RNA Viral , Esgotos , Águas ResiduáriasRESUMO
Phase synchronization is a universal concept in macro- and microstate coupling of complex systems. In this Rapid Communication, we demonstrate the existence of phase synchronization dynamics in a wave-particle interaction process under the framework of a Vlasov-Poisson (VP) system. Starting from a random phase setup, it is shown that the phases of the electrons spontaneously develop coherent patterns in its velocity space. The most interesting finding is that, mediated by the long-wavelength electrostatic mode, the phases of the electrons tend to attract each other, condense into a mean phase, and form a chimera pattern. Due to the finite-time singularity of the mean phase, the phase pattern gets evaporated and recondenses into a different chimera pattern. For the scenario of a finite wavelength, the random phases rapidly condense to slashing lines, which become steeper and steeper through a sequence of evaporation-condensation processes. The slashing pattern drives the mixing of the distribution function in the velocity space, which then induces the damping of the electrostatic mode. This phase self-organization dynamics uncovers another aspect of wave-particle interaction physics, and sheds light onto the longstanding enigma of Landau damping.
RESUMO
Luteoloside (luteolin-7-O-glucoside), the biomarker of Lonicera japonica, was efficiently bio-synthetized from its cheaper precursor luteolin. The structure of luteoloside was characterized by LC-MS and NMR analyses. Compared to the significant inhibitory effect of luteolin on human hepatocyte cell line LO2 at high doses, luteoloside did not show obvious cytotoxic effects at any test dose. Moreover, luteoloside exhibited obvious promotive effects on human hepatocyte cells, suggesting a potential application in hepatoprotective therapies.
