Light control of a movable microbubble in an ethanol-filled fiber microcavity for displacement measurement.

An optical fiber displacement sensor based on a light-controlled microbubble in an ethanol-filled fiber microcavity is proposed. The single-frequency 1550 nm laser in the side-lead-in single-mode fiber (SMF) produces an uneven temperature gradient by side irradiation in the ethanol-filled fiber microcavity. The microbubble shifts to the laser irradiation position along the microcavity due to the Marangoni effect and finally stops at the laser irradiation position. When the side-lead-in SMF moves, the microbubble follows. The surfaces of the microcavity and microbubble form a Fabry-Perot interferometer (FPI). The optical path difference (OPD) of the FPI is demodulated by the position of the side-lead-in SMF, which can be used for the displacement measurement with ultrahigh sensitivity (1.1 × 10-3 nm-1/µm). What is more, the proposed structure is only sensitive to a one-dimensional direction and has the advantages of non-contact, large range, and high resolution, which makes it a perfect candidate for displacement sensors.

Optical fiber inclinometer with dynamically controllable excitation length of quantum dots liquid-core waveguide based on a photo-controlled bubble.

An ultracompact fiber inclinometer based on a bubble controlled by Marangoni force is proposed in this Letter. By coupling a 980-nm laser, the bubble can suspend in a quantum dots (QDs) liquid-core waveguide (LCW) due to the Marangoni effect. Under the excitation of a 405-nm laser, QDs LCW exhibit green emissions centered at 523 nm. When the tilt angle changes, the position of the bubble changes as well, which causes the variation of the 523-nm fluorescence intensity. The experimental results show that the sensitivity based on the peak intensity ratio (PIR) reaches 0.22/° with a linearity of 0.979 from 0° to 35°. Furthermore, the sensor has excellent stability and repeatability.

Optical fiber sensor based on upconversion luminescence for synchronous temperature and curvature sensing.

A multifunctional optical fiber sensor based upconversion luminescence (UCL) for synchronous temperature and curvature sensing was proposed. The sensor was fabricated by assembling UCL nanoparticles doped by rare earth (RE) ions in polydimethylsiloxane (PDMS) materials. Temperature measurements were achieved through the fluorescent intensity ratio (FIR) technique with the dual green UC luminescence generated. The fabricated sensor provided the temperature sensitivity of 714.82 K-1 with excellent linearity (R2=0.997) at a temperature range of 303 to 423 K. In the lower temperature region, temperature measurement based on the FIR technology is almost independent on the fluorescence intensity of a 525 nm emission peak. Hence, deformation produced by the sensor through bending results in detectable and reversible changes in its reflected light, allowing the curvature to be simultaneously measured. The sensor can monitor temperature and curvature simultaneously, providing a new optical alternative for multi-parameters monitoring in the future.

Interplay of cGAS with micronuclei: Regulation and diseases.

In higher eukaryotes, sophisticate regulation of genome function requires all chromosomes to be packed into a single nucleus. Micronucleus (MN), the dissociative nucleus-like structure frequently observed in aging and multiple disease settings, has critical, yet under-recognized, pathophysiological functions. Micronuclei (MNi) have recently emerged as major sources of cytosolic DNA that can activate the cGAS-STING axis in a cell-intrinsic manner. However, MNi induced from different genotoxic stressors display great heterogeneity in binding or activating cGAS and the signaling responses downstream of the MN-induced cGAS-STING axis have divergent outcomes including autoimmunity, autoinflammation, metastasis, or cell death. Thus, full characterization of molecular network underpinning the interplay of cGAS and MN is important to elucidate the pathophysiological roles of immunogenic MN and design improved drugs that selectively target cancer via boosting the MN-derived cGAS-STING axis. Here, we summarize our current understanding of the mechanisms for self-DNA discrimination by cGAS. We focus on discussing how MN immunogencity is dictated by multiple mechanisms including integrity of micronuclear envelope, state of nucleosome and DNA, competitive factors, damaged mitochondrial DNA and micronucleophagy. We also describe emerging links between immunogenic MN and human diseases including cancer, neurodegenerative diseases and COVID-19. Particularly, we explore the exciting concept of inducing immunogenic MN as a therapeutic approach in treating cancer. We propose a new theoretical framework to describe immunogenic MN as a biological sensor to modulate cellular processes in response to genotoxic stress and provide perspectives on developing novel experimental approaches to unravel the complexity of MN immunogenicity regulation and immunogenic MN pathophysiology.

Sensitivity-Bandwidth Optimization of PMUT with Acoustical Matching Using Finite Element Method.

A new model in finite element method to study round-trip performance of piezoelectric micromachined ultrasonic transducers (pMUTs) is established. Most studies on the performance of pMUT are based only on the transmission sensibility, but the reception capacity is as much important as the transmission one, and is quite different from this latter. In this work, the round-trip sensitivity of pMUT is defined as the product of the frequency response of transmitted far field pressure to source voltage excitation and that of reception output to return wave pressure. Based on this sensitivity characteristic, firstly, a multi-parameter optimization for a cavity pMUT is performed using the sensitivity-bandwidth product parameter SBW as criterion. The radii of the electrode and the piezoelectric layer, the thicknesses of the piezoelectric layer and the vibration diaphragm are adjusted to maximize the performance. Secondly, an acoustic matching method is proposed and applied to pMUTs for the first time. As a result, the round-trip sensitivity can be evaluated and the pulse-echo response of wide-band excitation can be simulated, giving the most quantitative and intuitive feedback for pMUT design. The optimization enhances the sensitivity-bandwidth product by 52% when the top electrode and piezoelectric layer are both etched to 75% radius of the cavity beneath; the introduction of an acoustic matching layer shows significant bandwidth expansion in both the transmitting and receiving process.

Development of Broadband High-Frequency Piezoelectric Micromachined Ultrasonic Transducer Array.

Piezoelectric micromachined ultrasonic transducers (PMUT) are promising elements to fabricate a two-dimensional (2D) array with a pitch small enough (approximately half wavelength) to form and receive arbitrary acoustic beams for medical imaging. However, PMUT arrays have so far failed to combine the wide, high-frequency bandwidth needed to achieve a high axial resolution. In this paper, a polydimethylsiloxane (PDMS) backing structure is introduced into the PMUTs to improve the device bandwidth while keeping a sub-wavelength (λ) pitch. We implement this backing on a 16 × 8 array with 75 µm pitch (3λ/4) with a 15 MHz working frequency. Adding the backing nearly doubles the bandwidth to 92% (-6 dB) and has little influence on the impulse response sensitivity. By widening the transducer bandwidth, this backing may enable using PMUT ultrasonic arrays for high-resolution 3D imaging.

Polystoma luohetong n. sp. (Monogenea: Polystomatidae) from Rana chaochiaoensis Liu (Amphibia: Ranidae) in China.

Polystoma chaochiaoensis from the urinary bladder of the chaochiao frog Rana chaochiaoensis Liu was briefly described in a symposium abstract and presented at the Third Symposium on Parasitology of China in 1990. Types were not assigned and the original specimens collected are no longer available. The morphological description was incomplete and no illustrations were provided. We consider Polystoma chaochiaoensis a nomen nudum and provide a full description for the species and assign types. Based on morphological characteristics and molecular data of partial 18S rDNA sequences, we describe this species as Polystoma luohetong n. sp. Out of 578 frogs examined, 16 male and 38 female frogs were infected (prevalence 9.3%; mean intensity 1.02). Polystoma luohetong n. sp. is distinguished from all other Polystoma species by the presense of a prominent crest on the hamulus as well as by the shape and size of marginal hooklets and the intestinal arrangement. Furthermore, the phylogentic analysis based on the 18S rRNA gene shows Polystoma luohetong n. sp. well nested within the Ploystoma clade and as a sister taxon to Polystoma integerrimum.

Patients with COVID-19 in 19 ICUs in Wuhan, China: a cross-sectional study.

BACKGROUND: A COVID-19 outbreak started in Wuhan, China, last December and now has become a global pandemic. The clinical information in caring of critically ill patients with COVID-19 needs to be shared timely, especially under the situations that there is still a largely ongoing spread of COVID-19 in many countries. METHODS: A multicenter prospective observational study investigated all the COVID-19 patients received in 19 ICUs of 16 hospitals in Wuhan, China, over 24 h between 8 AM February 2h and 8 AM February 27, 2020. The demographic information, clinical characteristics, vital signs, complications, laboratory values, and clinical managements of the patients were studied. RESULTS: A total of 226 patients were included. Their median (interquartile range, IQR) age was 64 (57-70) years, and 139 (61.5%) patients were male. The duration from the date of ICU admission to the study date was 11 (5-17) days, and the duration from onset of symptoms to the study date was 31 (24-36) days. Among all the patients, 155 (68.6%) had at least one coexisting disease, and their sequential organ failure assessment score was 4 (2-8). Organ function damages were found in most of the patients: ARDS in 161 (71.2%) patients, septic shock in 34 (15.0%) patients, acute kidney injury occurred in 57 (25.2%) patients, cardiac injury in 61 (27.0%) patients, and lymphocytopenia in 160 (70.8%) patients. Of all the studied patients, 85 (37.6%) received invasive mechanical ventilation, including 14 (6.2%) treated with extracorporeal membrane oxygenation (ECMO) at the same time, 20 (8.8%) received noninvasive mechanical ventilation, and 24 (10.6%) received continuous renal replacement therapy. By April 9, 2020, 87 (38.5%) patients were deceased and 15 (6.7%) were still in the hospital. CONCLUSIONS: Critically ill patients with COVID-19 are associated with a higher risk of severe complications and need to receive an intensive level of treatments. COVID-19 poses a great strain on critical care resources in hospitals. TRIAL REGISTRATION: Chinese Clinical Trial Registry, ChiCTR2000030164. Registered on February 24, 2020, http://www.chictr.org.cn/edit.aspx?pid=49983&htm=4.

3D FEM Analysis of High-Frequency AlN-Based PMUT Arrays on Cavity SOI.

This paper presents three-dimensional (3D) models of high-frequency piezoelectric micromachined ultrasonic transducers (PMUTs) based on the finite element method (FEM). These models are verified with fabricated aluminum nitride (AlN)-based PMUT arrays. The 3D numerical model consists of a sandwiched piezoelectric structure, a silicon passive layer, and a silicon substrate with a cavity. Two types of parameters are simulated with periodic boundary conditions: (1) the resonant frequencies and mode shapes of PMUT, and (2) the electrical impedance and acoustic field of PMUT loaded with air and water. The resonant frequencies and mode shapes of an electrically connected PMUT array are obtained with a laser Doppler vibrometer (LDV). The first resonant frequency difference between 3D FEM simulation and the measurement for a 16-MHz PMUT is reasonably within 6%, which is just one-third of that between the analytical method and the measurement. The electrical impedance of the PMUT array measured in air and water is consistent with the simulation results. The 3D model is suitable for predicting electrical and acoustic performance and, thus, optimizing the structure of high-frequency PMUTs. It also has good potential to analyze the transmission and reception performances of a PMUT array for future compact ultrasonic systems.

Nonlinear Acoustic Modeling and Measurements during the Fatigue Process in Metals.

The nonlinear spring model combined with dislocation dipole theory was applied to describe the acoustic nonlinearity during the fatigue process in metals. The spring stiffness changes with fatigue degree. For the early stage, spring stiffness approaches infinity, and the heavier nonlinearity mainly results from the increase of dislocation density. Further fatigue leads to the occurrence of micro-cracks, during which spring stiffness begins to decrease. Abundant micro-crack sprouting accelerates the crack's expansion, and spring stiffness drops quickly, which causes the obvious decline in the transmitted harmonic amplitudes. Solutions obtained from the nonlinear wave equation with dislocation terms were added into the spring model. Varying spring stiffness was chosen for simulating the fatigue process. Then, nonlinear harmonic variation during this process was observed, which was classified into three stages: (I) the early dislocation fatigue stage; (II) the micro-crack sprouting stage; (III) the crack expansion stage. Nonlinear acoustic measurements were carried out on an aluminum alloy specimen during its fatigue process until cracks could be seen clearly. Harmonic variations in experiments can also be classified into the same three stages as the numerical results, which provides a theoretical and experimental reference for fatigue evaluation in metals using the nonlinear acoustic method.

Paradiplozoon yunnanensis n. sp. (Monogenea, Diplozoidae) from Sikukia gudgeri (Cyprinidae, Barbinae) in southwest China.

Paradiplozoon yunnanensis n. sp. (Monogenea, Diplozoidae) is described from the gills of Sikukia gudgeri Smith, 1931 (Cyprinidae) collected from Jinghong Basin, a tributary of the international Lancang-Mekong River. This is the first diplozoid species from S. gudgeri and its description increases the number of Paradiplozoon species recorded in China to 25. The new species is distinguished from congeners by a combination of morphological and molecular features. The anterior end of the median plate is thickened in the marginal area and a narrow rectangular trapeze spur connects to the anterior jaw through two separate anterior joining sclerites. The posterior end of the median plate sclerite is invaginated with a smooth strip-shaped posterior joining sclerite. Comparison of a newly obtained sequence of rRNA ITS2 with 18 other congeneric sequences from GenBank provides support for separation of the new species.

Association between the MTHFR C677T polymorphism, blood folate and vitamin B12 deficiency, and elevated serum total homocysteine in healthy individuals in Yunnan Province, China.

BACKGROUND: An increased serum total homocysteine (tHcy) concentration is typically associated with genetic defects involved in Hcy metabolism or related nutritional deficiencies. In this study, the combined effects of methylenetetrahydrofolate reductase (MTHFR) C677T polymorphism and folate and vitamin B12 deficiency on serum total Hcy (tHcy) levels were evaluated in a healthy Chinese population in Yunnan Province, China. METHODS: The MTHFR C677T polymorphism was genotyped in 330 volunteers (164 men and 166 women) using polymerase chain reaction-restriction fragment length polymorphism analysis. Folate, vitamin B12, and tHcy concentrations were determined by corpuscle immune chemiluminescence assays. The tHcy concentration was determined using an enzymatic assay. RESULTS: Significant negative correlations (p<0.001) were observed between the serum levels of tHcy and folate (r=-0.252) and vitamin B12 (r=-0.243). Men had significantly higher serum tHcy concentrations than women (p<0.001). Individuals with the MTHFR TT genotype had significantly higher serum tHcy concentrations than individuals with the CC and CT genotypes (p<0.001). The folate level of red blood cells was significantly increased in individuals with the TT genotype than in individuals with the CC genotype (p<0.05). Moreover, in the low vitamin group, the serum tHcy level was significantly correlated with the levels of folate (r=-0.334, p=0.001) and vitamin B12 (r=-0.212, p=0.046). CONCLUSION: The MTHFR C677T polymorphism, folate deficiency, and B12 deficiency were significantly associated with elevated serum tHcy levels. Among these three factors, folate deficiency had the greatest contribution to the serum tHcy concentration, followed by (in order of decreasing effect) MTHFR C677T and vitamin B12 deficiency. Thus, folic acid and vitamin B12 supplementation could help prevent diseases associated with tHcy accumulation, especially in individuals with the MTHFR 677TT genotype.

The Role of Genetic Polymorphisms as Related to One-Carbon Metabolism, Vitamin B6, and Gene-Nutrient Interactions in Maintaining Genomic Stability and Cell Viability in Chinese Breast Cancer Patients.

Folate-mediated one-carbon metabolism (FMOCM) is linked to DNA synthesis, methylation, and cell proliferation. Vitamin B6 (B6) is a cofactor, and genetic polymorphisms of related key enzymes, such as serine hydroxymethyltransferase (SHMT), methionine synthase reductase (MTRR), and methionine synthase (MS), in FMOCM may govern the bioavailability of metabolites and play important roles in the maintenance of genomic stability and cell viability (GSACV). To evaluate the influences of B6, genetic polymorphisms of these enzymes, and gene-nutrient interactions on GSACV, we utilized the cytokinesis-block micronucleus assay (CBMN) and PCR-restriction fragment length polymorphism (PCR-RFLP) techniques in the lymphocytes from female breast cancer cases and controls. GSACV showed a significantly positive correlation with B6 concentration, and 48 nmol/L of B6 was the most suitable concentration for maintaining GSACV in vitro. The GSACV indexes showed significantly different sensitivity to B6 deficiency between cases and controls; the B6 effect on the GSACV variance contribution of each index was significantly higher than that of genetic polymorphisms and the sample state (tumor state). SHMT C1420T mutations may reduce breast cancer susceptibility, whereas MTRR A66G and MS A2756G mutations may increase breast cancer susceptibility. The role of SHMT, MS, and MTRR genotype polymorphisms in GSACV is reduced compared with that of B6. The results appear to suggest that the long-term lack of B6 under these conditions may increase genetic damage and cell injury and that individuals with various genotypes have different sensitivities to B6 deficiency. FMOCM metabolic enzyme gene polymorphism may be related to breast cancer susceptibility to a certain extent due to the effect of other factors such as stress, hormones, cancer therapies, psychological conditions, and diet. Adequate B6 intake may be good for maintaining genome health and preventing breast cancer.

Safety research of extracorporeal membrane oxygenation treatment on cardiogenic shock: a multicenter clinical study.

BACKGROUND: Study the survival rate of extracorporeal membrane oxygenation (ECMO) and control group to treat cardiogenic shock and the complication and safety of the ECMO. METHODS: We collected patients with cardiogenic shock in the ICU of six hospitals from March 2013 to November 2014. There were 29 cases in the ECMO treatment group and 28 cases in the control group. The survival rate between ECMO treatment group and control group for 30 days and the occurrence rate of complication in the ECMO treatment group were compared. RESULTS: For the 29 patients in the ECMO group, 11 patients survived (with a survival rate of 37.9%) for 30 days, and 18 patients died (with a mortality rate of 62.1%). For the 28 patients in the control group, three patients survived (with a survival rate of 10.7%) and 25 patients died (with a mortality rate of 89.3%). Fifteen patients succeeded in separating from the ECMO machine within 14 days in the ECMO group. There were 11 patients that survived and left the hospital, with the time of 272.91±397.98 hours on ECMO, and 14 patients could not separate from the machine and died. For complications in the ECMO group, 11 patients had hemorrhaging (37.93%), 5 patients with neurological complications (17.21%), 1 patient with pneumothorax (3.44%), 8 patients with renal failure (27.59%), 12 patients with heart complication (41.38%), 9 patients with infection (31.03%), 7 patients with hyperbilirubinemia (24.14%). Twenty nine patients on the ECMO all used ventilator for support, among which, 17 patients adopted intra-aortic balloon pump (IABP) simultaneously, 8 patients with continuous hemofiltration due to renal failure (27.59%), for the death patients in ECMO group, 12 patients died from cardiogenic shock (48%), 3 patients with brain death (12%), 2 patients with septic shock (8%), and one patient with multiple organ failure (4%). CONCLUSIONS: The ECMO treatment can decrease the mortality rate with a low occurrence rate of complication, effective to rescue cardiogenic shock.

Metagenomic analysis of the Rhinopithecus bieti fecal microbiome reveals a broad diversity of bacterial and glycoside hydrolase profiles related to lignocellulose degradation.

BACKGROUND: The animal gastrointestinal tract contains a complex community of microbes, whose composition ultimately reflects the co-evolution of microorganisms with their animal host and the diet adopted by the host. Although the importance of gut microbiota of humans has been well demonstrated, there is a paucity of research regarding non-human primates (NHPs), especially herbivorous NHPs. RESULTS: In this study, an analysis of 97,942 pyrosequencing reads generated from Rhinopithecus bieti fecal DNA extracts was performed to help better understanding of the microbial diversity and functional capacity of the R. bieti gut microbiome. The taxonomic analysis of the metagenomic reads indicated that R. bieti fecal microbiomes were dominated by Firmicutes, Bacteroidetes, Proteobacteria and Actinobacteria phyla. The comparative analysis of taxonomic classification revealed that the metagenome of R. bieti was characterized by an overrepresentation of bacteria of phylum Fibrobacteres and Spirochaetes as compared with other animals. Primary functional categories were associated mainly with protein, carbohydrates, amino acids, DNA and RNA metabolism, cofactors, cell wall and capsule and membrane transport. Comparing glycoside hydrolase profiles of R. bieti with those of other animal revealed that the R. bieti microbiome was most closely related to cow rumen. CONCLUSIONS: Metagenomic and functional analysis demonstrated that R. bieti possesses a broad diversity of bacteria and numerous glycoside hydrolases responsible for lignocellulosic biomass degradation which might reflect the adaptations associated with a diet rich in fibrous matter. These results would contribute to the limited body of NHPs metagenome studies and provide a unique genetic resource of plant cell wall degrading microbial enzymes. However, future studies on the metagenome sequencing of R. bieti regarding the effects of age, genetics, diet and environment on the composition and activity of the metagenomes are required.

Mechanical and electrical properties of electrospun PVDF/MWCNT ultrafine fibers using rotating collector.

Poly(vinylidene fluoride) (PVDF) ultrafine fibers with different proportions of multi-walled carbon nanotube (MWCNT) embedded have been fabricated using a modified electrospinning device with a rotating collector. With the increasing of MWCNT content, the ß phase was noticeable enhanced, and the fibers became more elastic, which was manifested by Young's modulus decreased drastically. Furthermore, with adding the amounts of MWCNTs, the density of carbon nanotube (CNT)-CNT junctions among the fibers increased accordingly. When the MWCNT content was of 1.2 wt.%, a stable three-dimensional conducting network was formed. After this percolation threshold, the density of CNT-CNT junctions among the fibers tended to be a constant quantity, leading to a stabilized conductivity consequently. It is hoped that our results can be helpful for the fabrication of flexible devices, piezoelectric devices, force transducer, and so on. PACS: 81.05.Qk; 81.16.-c.

Plasma homocysteine levels and genetic polymorphisms in folate metablism are associated with breast cancer risk in chinese women.

BACKGROUND: Folate plays a pivotal role in DNA synthesis, repair, methylation and homocysteine (Hcy) metabolism. Therefore, alterations in the folate-mediated one-carbon metabolism may lead to abnormal methylation proliferation, increases of tumor/neoplasia and vein thrombosis/cardiovascular risk. The serine hydroxymethyhransferase (SHMT), methionine synthase (MS), methionine synthase reductase (MTRR) and cystathionine beta synthase (CBS) regulate key reactions in the folate and Hcy metabolism. Therefore, we investigated whether the genetic variants of the SHMT, MS, MTRR and CBS gene can affect plasma Hcy levels and are associated with breast cancer risk. METHODS: Genotyping was performed by PCR-RFLP method. Plasma Hcy levels were measured by the fluorescence polarization immunoassay on samples of 96 cases and 85 controls. RESULTS: (a) The SHMT 1420 T, MS 2756G, MTRR 66G allele frequency distribution showed significant difference between case and controls (p < 0.01 ~ 0.05). (b) The concentration of plasma Hcy levels of SHMT 1420TT was significantly lower than that of the wild type, while the plasma Hcy levels of MS 2756GG, CBS 699TT/1080TT significantly higher than that of the wild type both in case and controls. The plasma Hcy levels of MTRR 66GG was significantly higher than that of wild type in cases. The plasma Hcy levels of the same genotype in cases were significantly higher than those of controls except SHMT 1420CC, MS 2756AA, MTRR 66GG; (c) Multivariate Logistic regression analysis showed that SHMT C1420T (OR = 0.527, 95% CI = 0.55 ~ 1.24), MS A2756G (OR = 2.32, 95% CI = 0.29 ~ 0.82), MTRR A66G (OR = 1.84, 95% CI = 0.25 ~ 1.66) polymorphism is significantly associated with breast cancer risk. And elevated plasma Hcy levels were significantly linked to increased risk of breast cancer (adjusted OR = 4.45, 95% CI = 1.89-6.24 for the highest tertile as compared with the lowest tertile). CONCLUSIONS: The current study results seem to suggest a possibility that SHMT C1420T mutation may be negatively correlated with breast cancer susceptibility; while MS A2756G and MTRR A66G mutation may be positively associated with breast cancer risk. SHMT C1420T, MS A2756G, MTRR A66G, CBS C1080T, CBS C699T locus mutation may be factors affecting plasma levels of Hcy. The plasma Hcy levels could be metabolic risk factor for breast cancer risk to a certain extent.

Metagenomic analysis of the pygmy loris fecal microbiome reveals unique functional capacity related to metabolism of aromatic compounds.

The animal gastrointestinal tract contains a complex community of microbes, whose composition ultimately reflects the co-evolution of microorganisms with their animal host. An analysis of 78,619 pyrosequencing reads generated from pygmy loris fecal DNA extracts was performed to help better understand the microbial diversity and functional capacity of the pygmy loris gut microbiome. The taxonomic analysis of the metagenomic reads indicated that pygmy loris fecal microbiomes were dominated by Bacteroidetes and Proteobacteria phyla. The hierarchical clustering of several gastrointestinal metagenomes demonstrated the similarities of the microbial community structures of pygmy loris and mouse gut systems despite their differences in functional capacity. The comparative analysis of function classification revealed that the metagenome of the pygmy loris was characterized by an overrepresentation of those sequences involved in aromatic compound metabolism compared with humans and other animals. The key enzymes related to the benzoate degradation pathway were identified based on the Kyoto Encyclopedia of Genes and Genomes pathway assignment. These results would contribute to the limited body of primate metagenome studies and provide a framework for comparative metagenomic analysis between human and non-human primates, as well as a comparative understanding of the evolution of humans and their microbiome. However, future studies on the metagenome sequencing of pygmy loris and other prosimians regarding the effects of age, genetics, and environment on the composition and activity of the metagenomes are required.

Interactions between MTHFR C677T-A1298C variants and folic acid deficiency affect breast cancer risk in a Chinese population.

BACKGROUND: Our objective was to evaluate the MTHFR C677T-A1298C polymorphisms in patients with breast cancer and in individuals with no history of cancer, to compare the levels of genetic damage and apoptosis under folic acid (FA) deficiency between patients and controls, and to assess associations with breast cancer. METHODS: Genetic damage was marked by micronucleated binucleated cells (MNBN) and apoptosis was estimated by cytokinesis-block micronucleus assay (CBMN). PCR-RFLP molecular analysis was carried out. RESULTS: The results showed significant associations between the MTHFR 677TT or the combined MTHFR C677T-A1298C and breast cancer risk (OR=2.51, CI=0.85 to 7.37, p=0.08; OR=4.11, CI=0.78 to 21.8, p<0.001). The MNBN from the combined MTHFR C677T-A1298C was higher and the apoptosis was lower than that of the single variants (p<0.05). At 15 to 60 nmol /L FA, the MNBN in cases with the TTAC genotype was higher than controls (p<0.05), whereas no significant difference in apoptosis was found between the cases and controls after excluding the genetic background. CONCLUSIONS: Associations between the combined MTHFR C677T-A1298C polymorphism and breast cancer are possible from this study. A dose of 120 nmol/L FA could enhance apoptosis in cases with MTHFR C677T-A1298C. Breast cancer individuals with the TTAC genotype may be more sensitive to the genotoxic effects of FA deficiency than controls.

Choline and/or folic acid deficiency is associated with genomic damage and cell death in human lymphocytes in vitro.

Choline and folate are interrelated methyl donors. Previous studies showed that folate prevents genomic damage in human lymphocytes in vitro; however, the association between choline and human genomic stability is uncertain. To explore the genotoxicity, cytotoxicity, and cytostatic effects and possible interactions of choline and/or folate deficiency on the human genome, lymphocytes from 6 volunteers were cultured in 18 combinations of choline (CC) and folic acid (FA) media for 9 days. The genotoxicity was evaluated by micronuclei, nucleoplasmic bridges, and nuclear buds in the binucleated cell; the cytotoxicity indices included apoptosis and necrosis, and the cytostatic effects were indicated by nuclear division index (NDI). Across all choline concentrations, the frequencies of all biomarkers except NDI were diminished when FA concentration was more than or equal to 120 nmol/L. The frequencies of micronuclei, buds, and necrosis were significantly higher at lower levels of CC (0-6 µmol/L) compared with higher concentrations of CC (12-21.5 µmol/L) while maintaining the same FA concentration. We concluded that both choline and folate significantly impact genomic stability and cell death, although effects of folate were 2.5- to 6.2-fold greater, depending on the biomarker and dose. A combination of 12 µmol/L CC and 120 nmol/L FA appears to be optimal for genomic integrity in vitro.