Systematic investigation of the material basis, effectiveness and safety of Thesium chinense Turcz. and its preparation Bairui Granules against lung inflammation.

BACKGROUND: Thesium chinense Turcz. (Named as Bai Rui Cao in Chinese) and its preparations (e.g., Bairui Granules) have been used to treat inflammatory diseases, such as acute mastitis, lobar pneumonia, tonsillitis, coronavirus disease 2019 (COVID-19), and upper respiratory tract infection. However, the material basis, pharmacological efficiency, and safety have not been illustrated. METHODS: Anti-inflammatory activity-guided isolation of constituents has been performed using multiple column chromatography, and their structures were elucidated by NMR spectroscopy and ECD calculations. The inhibitory effects on lung inflammation and safety of the crude ethanol extract (CE), Bairui Granules (BG), and the purified active constituents were evaluated using lipopolysaccharide (LPS)-stimulated acute lung inflammation (ALI) mice model or normal mice. RESULTS: Seven new compounds (1-7) and fifty-six known compounds (8-63) were isolated from T. chinense, and fifty-four were reported from this plant for the first time. The new flavonoid glycosides 1-2, new fatty acids 4-5, new alkaloid 7 as well as the known constituents including flavonoid aglycones 8-11, lignans 46-54, alkaloids 34 and 45, coumarins 57, phenylpropionic acids 27, and simple aromatic compounds 39, 44 and 58 exhibited anti-inflammatory activity. Network pharmacology analysis indicated that anti-inflammation of T. chinense was attributed to flavonoids and alkaloids by regulating inflammation-related proteins (e.g., TNF, NF-κB, TGF-ß). Furthermore, constituents of T. chinense including kaempferol-3-O-glucorhamnoside (KN, also named as Bairuisu I, 19), astragalin (AG, Bairuisu II, 12), and kaempferol (KF, Bairuisu III, 8), as well as CE and BG could alleviate lung inflammation caused by LPS in mice by preventing neutrophils infiltration and the expression of the genes for pro-inflammatory cytokines NLRP3, caspase-1, IL-1ß, and COX-2. After a 28-day subacute toxicity test, BG at doses of 4.875 g/kg and 9.750 g/kg (equivalent to onefold and twofold the clinically recommended dose) and CE at a dose of 11.138 g/kg (equivalent to fourfold the clinical dose of BG) were found to be safe and non-toxic. CONCLUSIONS: The discovery of sixty-three constituents comprehensively illustrated the material basis of T. chinense. T. chinense and Bairui Granules could alleviate lung inflammation by regulating inflammation-related proteins and no toxicity was observed under the twofold of clinically used doses.

"Simmer pus and grow flesh" method promotes chronic wound healing in rats via bFGF-Wnt ß-catenin signaling pathway.

The purpose of this study was to explore the mechanism of "simmer pus and grow meat" method based on bFGF regulating WNT / ß-Catenin signaling pathway. Of 100 SPF rats, 25 were randomly selected as blank group, and 75 rats were established chronic infectious wound model and divided into blank group, model group (normal saline treatment, n = 25), experimental group (purple and white ointment treatment, n = 25), and wet burn ointment group (wet burn treatment, n = 25). The wound healing rate of rats was compared. The protein expressions of PCAN, VEGF, bFGF, ß-Catenin, GSK-3ß and C-Myc in granulation tissues were detected. On the 7th day, the wound healing rate of the model group was lower than that of the other 3 groups (P<0.05), and the wound healing rate of the positive control group was higher than that of the experimental group and the control group (P<0.05). The expressions of bFGF, GSK-3ß and C-MyC in model group were higher than those in control group (P<0.05). The ß-catenin protein expression in the model group was lower than that in the control group (P<0.05), and the ß-catenin protein expression in the experimental group and the positive control group was higher than that in the model group (P<0.05). The expressions of PCAN and VEGF in model group were lower than those in model group (P<0.05). We found that Zibai ointment promotes chronic wound healing by modulating the bFGF/Wnt/ß-Catenin signaling pathway.

Certifying Sets of Quantum Observables with Any Full-Rank State.

We show that some sets of quantum observables are unique up to an isometry and have a contextuality witness that attains the same value for any initial state. We prove that these two properties make it possible to certify any of these sets by looking at the statistics of experiments with sequential measurements and using any initial state of full rank, including thermal and maximally mixed states. We prove that this "certification with any full-rank state" (CFR) is possible for any quantum system of finite dimension d≥3 and is robust and experimentally useful in dimensions 3 and 4. In addition, we prove that complete Kochen-Specker sets can be Bell self-tested if and only if they enable CFR. This establishes a fundamental connection between these two methods of certification, shows that both methods can be combined in the same experiment, and opens new possibilities for certifying quantum devices.

Bounding the Amount of Entanglement from Witness Operators.

We present an approach to estimate the operational distinguishability between an entangled state and any separable state directly from measuring an entanglement witness. We show that this estimation also implies bounds on a variety of other well-known entanglement quantifiers. This approach for entanglement estimation is then extended to both the measurement-device-independent scenario and the fully device-independent scenario, where we obtain nontrivial but suboptimal bounds. The procedure requires no numerical optimization and is easy to compute. It offers ways for experimenters to not only detect, but also quantify, entanglement from the standard entanglement witness procedure.

Integrated network pharmacology and pharmacological investigations to explore the potential mechanism of Ding-Chuan-Tang against chronic obstructive pulmonary disease.

ETHNOPHARMACOLOGICAL RELEVANCE: Ding-Chuan-Tang (Abbreviated as DCT) is frequently prescribed for treatment of respiratory diseases, including chronic obstructive pulmonary disease (COPD), which is characterized by coughing, wheezing, and chest tightness in traditional Chinese medicine (TCM). However, the potential mechanism of DCT has not been investigated. AIM OF STUDY: The aim of the study is to explore the efficiency of DCT in the treatment of COPD in vivo and in vitro, and to illustrate the possible mechanism against COPD. METHODS: COPD model was induced by exposure of mice to cigarette smoke (CS) for 16 weeks. Enzyme-linked immunosorbent assay (ELISA), immunofluorescence assay, Western blot, etc., were used to explore the efficiency and mechanisms of DCT. Network pharmacology analysis, including Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis, etc., was performed to explore the potential targets in the treatment of DCT on COPD. RESULTS: DCT significantly alleviated pulmonary pathological changes in mouse COPD model, and inhibited inflammatory response induced by CS and LPS in vivo and in vitro. Network pharmacology analysis suggested that DCT alleviated COPD via inhibiting inflammation by regulating PI3K-AKT pathway. In cell-based models, DCT suppressed the phosphorylation of PI3K and AKT, which further regulated its downstream targets Nrf2 and NF-κB, and inhibited inflammatory response. CONCLUSIONS: DCT effectively attenuated COPD in the mouse model induced by CS. The therapeutic mechanism of DCT against COPD was closely associated with the regulation of PI3K-AKT pathway and its downstream transcription factors, Nrf2 and NF-κB.

Five new sesquiterpenoids isolated from Dictamnus dasycarpus Turcz.

Five new sesquiterpenoids, dictamtrinorguaianols E and F (1-2), and dictameudesmnosides F, G, and H (3-5), along with seven known sesquiterpenoids (6-12) were isolated from Dictamnus dasycarpus Turcz. The structures of all new compounds were characterized by spectroscopic methods, including UV, IR, HR-ESI-MS, and 1D and 2D NMR. The In-vitro anti-proliferative activities of all the compounds against two human cancer cell lines (SW982 and A549) were evaluated by CCK-8 assay. Compounds 1 and 4 showed medium anti-proliferative activity against SW982 cells, with IC50 values of 3.49 ± 0.10 and 6.42 ± 1.23 µM, respectively. Additionally, compounds 2, 7, and 8 exhibited medium anti-proliferative activity against A549 cells, with IC50 values ranging from 0.80 ± 0.05 to 6.60 ± 0.46 µM.

Supramolecular assembly of blue and green halide perovskites with near-unity photoluminescence.

The metal-halide ionic octahedron is the optoelectronic unit for halide perovskites, and a crown ether-assisted supramolecular assembly approach can pack various ionic octahedra into tunable symmetries. In this work, we demonstrate near-unity photoluminescence quantum yield (PLQY) blue and green emission with the supramolecular assembly of hafnium (Hf) and zirconium (Zr) halide octahedral clusters. (18C6@K)2HfBr6 powders showed blue emission with a near-unity PLQY (96.2%), and green emission was also achieved with (18C6@K)2ZrCl4Br2 powders at a PLQY of 82.7%. These highly emissive powders feature facile low-temperature solution-based synthesis conditions and maintain high PLQY in solution-processable semiconductor inks under ambient conditions, and they were used in thin-film displays and emissive three-dimensional-printed architectures that exhibited high spatial resolution.

Phenolic components from the fruits of Solanum xanthocarpum with anti-inflammatory activity.

Two new compounds (1 and 2), along with thirty-one known compounds (3-33) were isolated from the fruits of Solanum xanthocarpum. The structure of isolates was elucidated by analysis of spectroscopic data and the physicochemical methods. Meanwhile, the anti-inflammatory activity of isolates was determined using LPS-induced RAW 264.7 cells. The results of anti-inflammatory assays indicated that most isolated compounds (3, 4, 6, 8-14, 17-20, and 30) possessed significant nitric oxide (NO) production inhibition in lipopolysaccharide (LPS)-induced RAW 264.7 cells with IC50 values ranging from 14.33 to 48.55 µM.

Terpenoid compounds from the fruits of Solanum virginianum.

Eleven compounds were isolated and identified from ethanolic extracts of Solanum virginianum fruits, including two new compounds (1-2) and nine known compounds (3-11). Their structures were determined to be melongenaterpene C15-O-ß-D-glucopyranoside (1), (9Z)-3,7,11,15-tetramethyl -hexadeca-1,6,10-triene-3,5,14,15-tetraol-5-O-ß-D-glucopyranoside (2), actini-dioionoside A (3), byzantionoside B (4), citroside A (5), 7Z-roseoside (6), matenoside A (7), megastigmane (8), dihydrophaseic acid 3'-O-ß-D-glucopyranoside (9), taraxerol (10), and huzhangoside C (11). In this paper, NMR spectroscopy was used to study the structures of the compounds, comparing their data with those in the literature. In addition, the potential anti-inflammatory activity of the compounds was also evaluated using the RAW264.7 cell inflammation model induced by lipopolysaccharide (LPS). The terpenoids showed no significant anti-inflammatory activity.

Rapid inverse design of metamaterials based on prescribed mechanical behavior through machine learning.

Designing and printing metamaterials with customizable architectures enables the realization of unprecedented mechanical behaviors that transcend those of their constituent materials. These behaviors are recorded in the form of response curves, with stress-strain curves describing their quasi-static footprint. However, existing inverse design approaches are yet matured to capture the full desired behaviors due to challenges stemmed from multiple design objectives, nonlinear behavior, and process-dependent manufacturing errors. Here, we report a rapid inverse design methodology, leveraging generative machine learning and desktop additive manufacturing, which enables the creation of nearly all possible uniaxial compressive stress‒strain curve cases while accounting for process-dependent errors from printing. Results show that mechanical behavior with full tailorability can be achieved with nearly 90% fidelity between target and experimentally measured results. Our approach represents a starting point to inverse design materials that meet prescribed yet complex behaviors and potentially bypasses iterative design-manufacturing cycles.

[Contamination Characteristics and Risk Assessment of Polycyclic Aromatic Compounds in Surface Dust of Suntuan Mining Area in Huaibei].

The Suntuan mining area of Huaibei is a typical coal resource mining base in the Huainan-Huaibei areas in North China. Previous environmental studies related to surface dust in coal mining areas mainly focused on heavy metals and water-soluble ions, with little research on polycyclic aromatic compounds (PACs). In this study, gas chromatography-triple tandem quadrupole mass spectrometry (GC-MS/MS) was used to determine the contents of 16 parent polycyclic aromatic hydrocarbons (16PAHs), alkyl polycyclic aromatic hydrocarbons (aPAHs), and some oxygenated polycyclic aromatic hydrocarbons (OPAHs) in surface dust from the Suntuan mining area and surrounding environment. The results showed that the ΣPACs concentration range of surface dust in the Suntuan mining area was 283.8-36852.5 µg·kg-1 (mean:4114.2 µg·kg-1). ΣaPAHs (mean:2593.8 µg·kg-1) was 2.4 times higher than Σ16PAHs (mean:1074.9 µg·kg-1), which was the main contributor to PAC pollution. The composition of 16PAHs and aPAHs in surface dust was dominated by low molecular polycyclic aromatic hydrocarbons. The average OPAH content was 445.6 µg·kg-1. At the same time, PAC pollution was mainly concentrated around the mining area and near the road of a coal gangue landfill. Based on the positive matrix factor (PMF) analysis, it was inferred that the study area was mainly affected by petroleum sources, followed by coal and biomass combustion, and traffic sources and petroleum product leakage accounted for a relatively small proportion. Based on the ratio and distribution pattern of 16PAHs and aPAHs, it was inferred that when Σ16PAHs/ΣPACs<0.25, it was mainly polluted by the coal mining area. The results of PMF combined with lifetime carcinogenic risk (ILCR) showed that there were potential carcinogenic risks for children near the study area, mainly from coal and biomass burning and coal mining. There are many coal mining areas in Huaihe River Basin in China. The results of this study can provide reference for pollution prevention and control of PACs in these coal mining areas.

Indole-3-acetonitrile Is a Critical Molecule with Weed Allopathic Suppression Function in Broccoli (Brassica oleracea var. italica).

Cruciferous plants are frequently used for ecologically benign weed control in agricultural production. Most effective Broccoli varieties were screened using the entropy method-based topsis model at first. Result showed that varieties of Lvwawa and Lvbaoshiwere most effective in allelopathic suppression on radishes. Column and thin-layer chromatography were used to extract the allelopathic compounds from broccoli residues, which contained various herbicidal active substances; among them, purified single-molecule indole-3-acetonitrile has a stronger inhibitory effect than pendimethalin (commercial herbicide). The weed inhibition rate increased with increasing broccoli residue dosage, with a 40 g/m2 broccoli residue dose yielding the highest suppression rate. Its effect was similar to that of indole-3-acetic acid. Too much of this substance leads to the plant's death. Moreover, broccoli residues had effective control effect on weeds in natural soils in greenhouse and field trials. The results demonstrated that broccoli residue could be used for weed management in field for abundant allopathic suppression molecules to weeds, and that Indole-3-acetonitrile is one of the most important allopathic molecule.

Experimental Test of High-Dimensional Quantum Contextuality Based on Contextuality Concentration.

Contextuality is a distinctive feature of quantum theory and a fundamental resource for quantum computation. However, existing examples of contextuality in high-dimensional systems lack the necessary robustness required in experiments. Here, we address this problem by identifying a family of noncontextuality inequalities whose maximum quantum violation grows with the dimension of the system. At first glance, this contextuality is the single-system version of multipartite Bell nonlocality taken to an extreme form. What is interesting is that the single-system version achieves the same degree of contextuality but uses a Hilbert space of lower dimension. That is, contextuality "concentrates" as the degree of contextuality per dimension increases. We show the practicality of this result by presenting an experimental test of contextuality in a seven-dimensional system. By simulating sequences of quantum ideal measurements with destructive measurements and repreparation in an all-optical setup, we report a violation of 68.7 standard deviations of the simplest case of the noncontextuality inequalities identified. Our results advance the investigation of high-dimensional contextuality, its connection to the Clifford algebra, and its role in quantum computation.

Investigating the role of Zibai ointment on apoptosis-related factors Bcl-2 and Bax in wound healing after anal fistula surgery.

OBJECTIVE: In this study, we investigated the impact of Zibai ointment on wound healing by analyzing the expression levels of two key apoptosis-related factors-B-cell lymphoma 2 (Bcl-2) and Bcl-2-associated X protein (Bax), in patients following surgery for anal fistula. METHODS: We included 90 patients with anal fistulas who were treated in the People's Hospital Affiliated to Fujian University of Traditional Chinese Medicine. Patients were randomly assigned to receive treatment with Zibai ointment (n = 45) or petroleum jelly (n = 45). The levels of apoptosis-related factors Bcl-2 and Bax were evaluated using enzyme-linked immunosorbent assay (ELISA), while cell apoptosis was assessed using Terminal deoxynucleotidyl transferase (TdT) dUTP Nick-End Labeling (TUNEL) assay. RESULTS: The results of ELISA showed that on Day 21 after the surgery, the levels of Bcl-2 and Bax in the Zibai ointment group were significantly different compared to the petroleum jelly group, with values of (60.11 ± 1.31) ng/mL and (7.05 ± 0.01) versus (83.79 ± 1.74) ng/mL and (6.00 ± 0.05) ng/mL, respectively (p < .05). Furthermore, light microscopy revealed a large number of apoptotic cells within the field of vision 14 days postsurgery in the Zibai ointment group, and the healing time in the Zibai ointment group was significantly different from that in the petroleum jelly group (p < .05). CONCLUSION: We found that Zibai ointment effectively promoted wound healing in patients following anal fistula surgery, possibly by regulating Bcl-2 and Bax apoptosis-related factors.

Exploring the impact mechanism of executives' environmental attention on corporate green transformation: evidence from the textual analysis of Chinese companies' management discussion and analysis.

The allocation of executives' environmental attention (EEA) is of great significance in promoting the green upgrading of industrial structures and achieving corporate green transformation. Based on upper echelon theory and the attention-based view, we use panel data of Chinese manufacturing companies from 2015 to 2020 to construct a two-way fixed effects model to explore the impact mechanism of EEA on corporate green transformation performance (CGTP). Baseline regression shows that EEA significantly improves CGTP. The reliability of findings is verified by reducing time windows, replacing the independent variable, expanding the data source, and adding missing variables. In the heterogeneity analysis, the positive effect of EEA on CGTP is significant for eastern companies and does not differ in the property rights grouping. After propensity score matching, environmental attribute grouping shows that the positive effect of EEA on CGTP is more significant for non-heavy polluters. Extended research shows that government subsidies have a positive moderating effect, while female executives play only a symbolic role. Moreover, green innovation activities have positive partial mediating effects. Green innovation is the best way to address environmental pollution and achieve corporate green transformation. Our research provides implications for decision-makers to allocate their attention, and thereby achieve green development appropriately.

Quantum Contextuality Provides Communication Complexity Advantage.

Despite the conceptual importance of contextuality in quantum mechanics, there is a hitherto limited number of applications requiring contextuality but not entanglement. Here, we show that for any quantum state and observables of sufficiently small dimensions producing contextuality, there exists a communication task with quantum advantage. Conversely, any quantum advantage in this task admits a proof of contextuality whenever an additional condition holds. We further show that given any set of observables allowing for quantum state-independent contextuality, there exists a class of communication tasks wherein the difference between classical and quantum communication complexities increases as the number of inputs grows. Finally, we show how to convert each of these communication tasks into a semi-device-independent protocol for quantum key distribution.

Parent and alkylated polycyclic aromatic hydrocarbon emissions from coal seam fire at Wuda, Inner Mongolia, China: characteristics, spatial distribution, sources, and health risk assessment.

The Wuda coalfield in Inner Mongolia is a vital coal base in China, and it is the hardest-hit area for coal fires (spontaneous combustion of coal seams and coal gangue). Using gas chromatography-mass spectrometry, this work tested the concentration and analyzed the characteristics, distribution, sources, and health risks of polycyclic aromatic compounds (PACs) in the surface soil of the Wuda District, including the coal mine, coal fire, agricultural, and background areas. The soil of coal mine and coal fire area were heavily polluted with PACs, with mean concentrations of 9107 and 3163 µg kg-1, respectively, considerably higher than those in the agricultural (1232 µg kg-1) and background areas (710 µg kg-1). Alkyl polycyclic aromatic hydrocarbons (APAHs) were the dominant pollutants among these PACs, accounting for 60-81%. Alkyl naphthalenes and alkyl phenanthrenes are the primary pollutants in APAHs, accounting for 80-90% of the total amounts. Additionally, using the positive matrix factorization method, it can be concluded that the primary PAC sources are petrogenic sources, coal and biomass combustion, coal fires, and vehicle emissions. Finally, according to the cancer risk values of 16 PAHs, only the coal mine area showed a potential cancer risk. However, this result lacks a risk assessment of APAHs and underestimates the actual risk. The results of this study improved the understanding of PAC pollution in coal fire and surrounding areas and provided a reference for environmental and health risk investigations.

Contamination characteristics of polycyclic aromatic compounds from coal sources in typical coal mining areas in Huaibei area, China.

The Huaibei area is rich in coal resources and serves as the main energy production base in East China. However, serious environmental consequences are associated with coal mining and utilization. With increasing reports on distribution and risks by polycyclic aromatic compounds (PACs), the potential pollution of coal sources must be addressed. Here, the PAC concentrations in the topsoil, coal, and coal gangue of a typical coal mining area in Huaibei were evaluated. The mean ΣPACs in topsoil, coal, and coal gangue were 1528.3, 274,815.8, and 10,908.3 µg·kg-1, respectively. Alkyl polycyclic aromatic hydrocarbons (aPAHs) were identified as primary contributors to PACs, and the concentrations of oxygenated PAHs (oPAHs) were significantly higher in coal and coal gangue than in topsoil. PAC pollution was mainly concentrated in the coal mine area and near the coal gangue landfill road. Not only sixteen high priority pollutant PAHs (16PAHs), but PAH derivatives also contributed to the organic pollution from coal sources. Principal components analysis, multiple linear regression, characteristic ratios, and positive matrix factor analysis were used to trace PAC sources. The characteristic ratios for organic pollution from coal and gangue particles involving 16PAHs, aPAHs, and oPAHs were proposed. Further, the high-ring 16PAH ratio was also found suitable for coal mining areas. The Monte-Carlo risk assessment showed that coal particles were highly carcinogenic, and despite the low carcinogenicity of coal gangue and topsoil, they might also serve as potential carcinogens. This study aimed to disseminate knowledge on PACs from coal and coal gangue, provide a useful background for efficient resource utilization of coal gangue, and a reference for tracing PAC sources in coal mine environment media.

Contamination characteristics of alkyl polycyclic aromatic hydrocarbons in dust and topsoil collected from Huaibei Coalfield, China.

Alkyl polycyclic aromatic hydrocarbons (APAHs) are more toxic and persistent than their parent compounds. In this study, the concentrations of polycyclic aromatic compounds (PACs) in dust, topsoil and coal gangue from Huaibei Coal mine, China were analyzed by gas chromatography-mass spectrometry, confirming APAHs were the dominant pollutants. The mean concentrations of APAHs were substantially higher than those of 16 PAHs in both dust and topsoil. The mean concentration of APAHs in dust was 9197 µg kg-1, accounting for 80% of the total mean concentration of PACs. The mean concentration of APAHs in topsoil was 2835 µg kg-1, accounting for 77% of the mean concentration of PACs. Alkyl naphthalenes and alkyl phenanthrenes were the primary pollutants in APAHs. Their mean concentrations in dust and topsoil were 7782 µg kg-1 and 2333 µg kg-1, respectively. This accounted for 85% and 82% of the concentration of APAHs, respectively. Additionally, low-molecular-weight APAHs dominated the PACs of the coal mine, exhibiting petrogenic characteristics; distribution of C1-C4 NAP and C1-C4 PHE exhibited "bell shape" pattern indicated as petrogenic source. Source identification indicated that the PACs were mainly derived from petrogenic sources and vehicle emissions, followed by biomass and coal burning. Fingerprinting information of dust and topsoil were consistent with coal gangue, indicating that PACs are most likely derived from coal gangue. Coalfields comparable to our study area are widely distributed in China. Therefore, investigating PAC pollution derived from coal gangue warrants further attention.

Alkylated Polycyclic Aromatic Hydrocarbons Are the Largest Contributor to Polycyclic Aromatic Compound Concentrations in the Topsoil of Huaibei Coalfield, China.

Alkyl polycyclic aromatic hydrocarbons (APAHs) are more toxic and persistent than their parent compounds. Here, the concentrations, composition profiles, and spatial distribution of polycyclic aromatic compounds (PACs) in 127 topsoil samples from Huaibei coalfield were analyzed. The PAC concentrations in different functional areas were significantly different: mining area > industrial area > residential area > agricultural area. APAHs were the major contributors to PACs, accounting for 71-83% of total PACs. Alkylnaphthalenes and alkylphenanthrenes were the primary APAH components, accounting for 83-87% of APAHs. Principal component analysis showed that petrogenic source, coal and biomass combustion, and vehicle emissions were the primary sources of PACs. By comparing the fingerprint information of soil, coal, and coal gangue, it was hypothesized that the petrogenic source of PAC pollution in typical mining areas and surrounding areas are coal particle scattering and coal gangue weathering. Some coal mining and industrial areas potentially pose risks to children, whereas others do not. There are limited evaluation criteria for alkyl PAHs; hence, the estimated risk is likely lower than the actual risk. In addition to the conventional 16 PAHs, it is critical to consider a broader range of PACs, especially APAHs.