[Inhibiting effect and mechanism of Lactobacillus E6-1 on oral cancer cell line Cal-27].

PURPOSE: To study the effect of Lactobacillus E6-1 on proliferation and apoptosis of human oral cancer cell line Cal-27 in vitro. METHODS: MTT assay was carried out by different concentrations of Lactobacillus E6-1 on Cal-27 to detect the inhibitory effect on cell proliferation, and the effect on DNA was detected by agarose gel electrophoresis. The expression levels of cyt-c, caspase-9 and caspase-3 in each group was detected by Western blot. The data were analyzed with SPSS 21.0 software package. RESULTS: The results of MTT assay showed that E6-1 had obvious inhibitory effect on Cal-27 in a dose-dependent manner. DNA ladder in Cal-27 cells was induced by different concentrations of E6-1 (10, 20, 40 mg/mL). Compared with blank control group, Western blot results showed that the expression of cyt-c, caspase-9 and caspase-3 increased significantly(P<0.05). CONCLUSIONS: Lactobacillus E6-1 can inhibit the proliferation of Cal-27 and effectively induce apoptosis of tumor cells In vitro.

Copper catalyzed decarboxylative alkynylation of quaternary α-cyano acetate salts.

Cu-catalyzed decarboxylative alkynylation of quaternary α-cyano acetate salts with alkynyl bromides and alkynyl chlorides is described. This new reaction can be used for preparing functionalized butynenitrile derivatives.