RESUMO
The widespread use of copper and tetracycline as growth promoters in the breeding industry poses a potential threat to environmental health. Nevertheless, to the best of our knowledge, the potential adverse effects of copper and tetracycline on the gut microbiota remain unknown. Herein, mice were fed different concentrations of copper and/or tetracycline for 6 weeks to simulate real life-like exposure in the breeding industry. Following the exposure, antibiotic resistance genes (ARGs), potential pathogens, and other pathogenic factors were analyzed in mouse feces. The co-exposure of copper with tetracycline significantly increased the abundance of ARGs and enriched more potential pathogens in the gut of the co-treated mice. Copper and/or tetracycline exposure increased the abundance of bacteria carrying either ARGs, metal resistance genes, or virulence factors, contributing to the widespread dissemination of potentially harmful genes posing a severe risk to public health. Our study provides insights into the effects of copper and tetracycline exposure on the gut resistome and potential pathogens, and our findings can help reduce the risks associated with antibiotic resistance under the One Health framework.
Assuntos
Antibacterianos , Cobre , Microbioma Gastrointestinal , Tetraciclina , Animais , Cobre/toxicidade , Tetraciclina/farmacologia , Camundongos , Microbioma Gastrointestinal/efeitos dos fármacos , Antibacterianos/farmacologia , Resistência Microbiana a Medicamentos/genética , Bactérias/efeitos dos fármacos , Bactérias/genética , Fezes/microbiologiaRESUMO
<p><b>OBJECTIVE</b>To evaluate the clinical application of lung function analysis in diagnosis of children respiratory diseases.</p><p><b>METHODS</b>Respiratory function was evaluated with portable lung function detector in 6 261 children with respiratory diseases. FEV1, FVC and PEFR were measured in 268 children with cough variant asthma (CVA) and 147 children with asthma (AS), and the results were self-compared during follow-up.</p><p><b>RESULT</b>There were no significant differences in above parameters between children with cough variant asthma and those with asthma (P>0.05). Lung functions of asthma children were improved evidently by administration of glycocorticoid inhalator (P<0.001).</p><p><b>CONCLUSION</b>Lung function detection is essential for diagnosis of asthma and it can be used as a reliable index for therapeutic effect of asthma children.</p>
