Effects of dietary dandelion extract on intestinal morphology, antioxidant status, immune function and physical barrier function of juvenile golden pompano Trachinotus ovatus.

Intestinal morphology, antioxidant status, immune function and tight junction proteins mRNA expression were examined in golden pompano (Trachinotus ovatus) that fed respectively six diets containing dandelion extracts (DE) at 0, 0.5, 1, 2, 4 and 10 g kg-1 after 8 weeks feeding. The study indicated that dietary DE significantly improved intestinal antioxidant abilities by increasing SOD, CAT, T-AOC activities and up-regulating intestinal cat, gpx mRNA levels, but by decreasing MDA content and down-regulating intestinal keap1 mRNA levels in golden pompano. Meanwhile, dietary DE improved intestinal morphology, suggesting that enhances intestinal digestion and absorption, by increasing muscle thickness, villus length, villus width and villus number in the foregut and hindgut; as well as villus number, villus width and muscle thickness in the midgut (P < .05). Dietary DE enhanced intestinal barrier function by increasing intestinal zo-1 and occludin mRNA levels, but by decreasing the mRNA levels of claudin-12 and claudin-15. Furthermore, dietary DE improved intestinal immunity via increasing goblet cells numbers and regulating expression of immune-related genes. In conclusion, dietary DE supplementation promoted intestine health by improving intestine morphology, immunity, antioxidant abilities and intestinal barrier in golden pompano.

Effects of dietary hawthorn extract on growth performance, immune responses, growth- and immune-related genes expression of juvenile golden pompano (Trachinotus ovatus) and its susceptibility to Vibrio harveyi infection.

The present study was conducted to investigate the effects of dietary hawthorn extract (HTE) supplementation on growth performance, immune responses, hepatic antioxidant abilities, growth- and immune-related and heat shock protein genes expression and resistance to the pathogen Vibrio harveyi in Trachinotus ovatus. A basal diet supplemented with HTE at 0 (Diet 1), 0.50 (Diet 2), 1.00 (Diet 3), 2.00 (Diet 4), 4.00 (Diet 5) and 10.00 (Diet 6) g kg-1 were fed to golden pompano for 8 weeks. The highest final body weight, weight gain rate, specific growth rate, feed efficiency ratio and protein efficiency rate were observed in fish fed Diet 2 (P < 0.05). Dietary HTE significantly increased plasma complement 3, complement 4 and immunoglobulin M content (P < 0.05). Hepatic antioxidant enzymes (SOD, T-AOC, CAT, GPx, GR) significantly increased (P < 0.05), whereas MDA content decreased first and then increased in fish fed HTE supplement. After challenge with Vibrio harveyi, significant higher post-challenge survival was observed in fish fed Diet 2 and Diet 3 than the control group (P < 0.05). Transcription levels of growth-related genes (IGF-I and IGF-II) were significantly up-regulated in fish fed HTE supplement (P < 0.05), whereas HSP70 and HSP90 mRNA levels were significantly down-regulated (P < 0.05). With respect to immune-related genes, such as tumour necrosis factor-alpha (TNF-α), interleukin-8 (IL-8) and inhibitor protein κBα (IκB-α), upregulation was observed in the liver of fish fed with the diet supplemented with HTE. In contrast, the expression of antioxidant enzyme genes (CAT, GPx, MnSOD and Keap1) and cytokines (IL-10, TGF-ß1 and TOR) was downregulated. These results indicated that golden pompano fed a diet supplemented with 0.50 g kg-1 HTE could significantly promote growth performance and growth-related genes expression, strengthen immunity, and improve hepatic antioxidative abilities and resistance to Vibrio harveyi infection.