RESUMO
In the present study, scapaundulin C (1), a new labdane diterpenoid, and four related known compounds scapaundulin A (2), 5α, 8α, 9α-trihydroxy-13E-labden-12-one (3), 5α, 8α-dihydroxy-13E-labden-12-one (4), and (13S)-15-hydroxylabd-8 (17)-en-19-oic acid (5), were isolated from the Chinese liverwort Scapania undulate (L.) Dum., using column chromatography. The structures of these compounds were determined on the basis of 1D- and 2D-NMR analyses. The acetylcholinesterase (AchE) inhibitory activity was evaluated using a bioautographic TLC assay and the cytotoxic activity was evaluated by the MTT method. All the compounds were reported for the first time to exhibit moderate AchE inhibitory activity with minimal inhibitory quantities ranging from 250 to 500 ng. All the compounds were tested for their cytotoxicity against five human tumor cell lines, A549, K562, A2780, Hela, and HT29, and compounds 3 and 4 exhibited moderate inhibitory effects on the growth of A2780 cells.
Assuntos
Humanos , Acetilcolinesterase , Inibidores da Colinesterase , Química , Diterpenos , Química , Hepatófitas , Química , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Extratos Vegetais , QuímicaRESUMO
OBJECTIVE: To study the chemical constituent from the antithrombus activity extract of Radix Aerio Fici Microcarpae. METHODS: The isolation and purification of the extract were conducted by means of silica gel column chromatography, polyamide column chromatography and recrytallization. The structures of these compounds were elucidated based on the physicochemical properties and spectroscopic analysis. RESULTS: 7 compounds were isolated from the extract. They were identified as isovanillic acid (1), 4-hydroxybenzoic acid(2),3,4-dihydroxybenzoic acid (3), friedelane (4), alpha-amyrin acetate (5), lanosterol (6) and beta-sitosterol (7) . CONCLUSION: Compound 3,4 and 7 are isolated from this plant for the first time.
Assuntos
Fibrinolíticos/isolamento & purificação , Ficus/química , Hidroxibenzoatos/isolamento & purificação , Sitosteroides/isolamento & purificação , Triterpenos/isolamento & purificação , Fibrinolíticos/química , Hidroxibenzoatos/química , Lanosterol/química , Lanosterol/isolamento & purificação , Estrutura Molecular , Ácido Oleanólico/análogos & derivados , Ácido Oleanólico/química , Ácido Oleanólico/isolamento & purificação , Parabenos/química , Parabenos/isolamento & purificação , Raízes de Plantas/química , Sitosteroides/química , Triterpenos/química , Ácido Vanílico/análogos & derivados , Ácido Vanílico/química , Ácido Vanílico/isolamento & purificaçãoRESUMO
To study the chemical constituents of the traditional Chinese herb Baeckea Frutescens L., a new flavonol glycoside, named 6, 8-dimethylkaempferol-3-O-alpha-L-rhamnoside (1), together with seven known compounds: quercetin (2), quercetin-3-O-alpha-L-rhamnoside (3), myricetin (4), myricetin-3-O-alpha-L-rhamnoside (5), gallic acid (6), ursolic acid (7) and 1,3-dihydroxy-2-(2'-methoxylpropionyl)-5-methoxy-6-methylbenzene (8) were isolated by using silica gel column chromatography, polyamide column chromatography and recrytallization. Their structures were identified on the basis of physicochemical properties and spectroscopic analysis. Among them, compounds 2-7 were isolated from this plant for the first time and compound 8 was first isolated from plant.
