Evaluating the adhesion of human gingival fibroblasts and MG-63 osteoblast-like cells to activated PRP-coated membranes.

Regeneration of periodontal tissues is affected by the biological and morphological characteristics of the membrane surface. The current study evaluated the adhesion of human gingival fibroblasts (HGF) and MG-63 osteoblast-like cells to Membranes, with and without activated PRP. The line of human gingival fibroblast cells and MG-63 osteoblast-like cells were first prepared and cultured on three types of membranes, including Jason, CenoMembrane and TXT-200 in three groups (FBS 10%, FBS 0.5% and activated PRP). Cell viability was investigated by MTT assay and electron microscopy (SEM) was used to evaluate the cell morphology and adhesion on these membranes after 24 and 72 h. Two-way ANOVA was carried out at the significance level of 0.05. The highest adhesion in the 10% FBS group for HGF and The MG-63 osteoblast-like cells was observed to the Jason membrane during 24 h and 72 h (p < 0.05). However, there were no significant differences among the three membranes in PRP and FBS groups for HGF during 24 h and for MG-63 cells during 72 h (p > 0.05). Activated PRP had a positive effect on the viability and adhesion of both human gingival fibroblasts and osteoblast-like cells as compared to the FBS 0.5% group, but these effects were not as 10% FBS group. The results also showed that Jason membrane had the highest amount of cell viability and adhesion.

Cytotoxicity of Two Experimental Epoxy Resin-Based Sealers.

INTRODUCTION: Many endodontic sealers are available, but search for the ideal sealer continues. This study aimed to assess the cytotoxicity of two experimental endodontic sealers in comparison with AH-26 resin sealer. METHODS AND MATERIALS: This in vitro study was conducted on conventional and experimental root canal sealers: AH-26, an epoxy resin experimental sealer A (ES-A) composed of calcium tungstate, zirconium oxide, aerosil, bismuth oxide, titanium oxide, hexamine and an epoxy resin and experimental sealer B (ES-B) with compositions similar to ES-A except for the presence of imidazoline as a catalyst. The experimental sealers containing nano-particles were mixed with 37.5% of an epoxy resin. The extraction of five samples of each experimental sealer (A, B) and AH-26 sealer were subjected to MTT assay in the form of set and fresh at 1, 24 and 72 h with 1, 10, 100% dilution according to the International Standard ISO:10993-2012. Data were analyzed using the one-way ANOVA. RESULTS: The set ES-A had the least cytotoxicity from the first hour but the cytotoxicity of ES-B and AH-26 extraction decreased over time. In fresh form, except for 100% concentration, ES-A showed the least cytotoxicity compared to the other two sealers. CONCLUSION: All three sealers had high cytotoxicity in 100% concentration but had low cytotoxicity in 10% and 1% concentrations.