RESUMO
Using the cytochrome b gene (1143 bp), species identification and the phylogenetic analysis of voles of the generic group Microtus from the eastern part of the Greater Caucasus, including the Ismayilli, Khizi, and Balakan Districts of Azerbaijan, have been carried out. Three species, the Major's pine vole (M. majori), the social vole (M. socialis), and the common vole (M. arvalis form obscurus), have been identified, and five new haplotypes have been described for them. Genetic analysis with the inclusion of the new data showed that for each of the species, the physiographic conditions of the Greater Caucasus played a certain role (isolation, migration route or refugium) during the formation of the modern genetic structure. The obtained results indicate that any new data from the Caucasus could be of critical importance for the reconstruction of the evolutionary history of the modern biodiversity both within the region itself and in adjacent territories.
Assuntos
Arvicolinae , Animais , Arvicolinae/genética , Citocromos b/genética , Variação Genética/genética , FilogeniaRESUMO
The phylogenetic relationships of burbot (Lota lota L., 1758) of the Volga-Kama River basin are reconstructed for the first time. The sequences of the gene cytochrome b and the mtDNA control region obtained for 44 samples from the Kama River and Mezhevaya Utka River (the Volga River tributaries) are studied. New haplotypes of both markers were revealed. The results of phylogenetic reconstructions based on cytochrome b and control region mtDNA do not contradict the existing ideas about the phylogenetic structure of the species, and indicate inclusion of burbot from the Volga-Kama basin in Eurasian haplogroup. According to obtained data, the Volga-Kama River basin could play an important role in shaping the genetic diversity of burbot in Europe, and during certain periods it served as a corridor connecting the river systems of the European and Asian parts of the species range.
Assuntos
DNA Mitocondrial/genética , Gadiformes/genética , Marcadores Genéticos , Filogenia , Animais , Teorema de Bayes , Citocromos b/metabolismo , Europa (Continente) , Variação Genética , Geografia , Haplótipos , Músculo Esquelético/metabolismo , Rios , Análise de Sequência de DNARESUMO
The genetic variability of burbot (Lota lota L., 1758) inhabiting the Ob-Irtysh and Taz river basins in Western Siberia has been studied based on the polymorphism of the hypervariable fragment of mtDNA control region (407 bp). The analysis of 134 fish samples revealed 30 haplotypes, 23 of which were new. Among haplotypes, previously detected in Eurasia and North America, EB30 was the most frequently found in Western Siberia (45.5% frequency). The results of our study are in agreement with previous research pointing to the genetic differentiation of two burbot subspecies, L. l. lota and L. l. maculosa, and indicate that burbot inhabiting the Ob-Irtysh and Taz river basins belong to the Eurasian-Beringian clade (nominative subspecies L. l. lota). However, a high genetic diversity of burbot in Western Siberia, along with a relatively high differentiation of burbot groups within studied territory, points to a regional specificity of burbot population.
Assuntos
DNA Mitocondrial/genética , Gadiformes/genética , Polimorfismo Genético , Animais , SibériaRESUMO
Analysis of genetic diversity of burbot (Lota lota Linneus, 1758) mitochondrial control region (mtCR) haplotypes from geographically distant localities in the Ob-Irtysh River basin in comparison with distribution of known burbot haplotypes was conducted. mtCR fragments from burbot samples, obtained in two localities (longitudinal part of the Irtysh near Tobolsk and the Sob River, a left-bank tributary of the Ob River), were sequenced.