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1.
Rheumatology (Oxford) ; 63(2): 277-284, 2024 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-37594755

RESUMO

OBJECTIVE: The relationship between FMF and pregnancy outcomes remains unclear. This systematic review and meta-analysis aimed to clarify this association. METHODS: Electronic databases-PubMed, Web of Science, Cochrane, and EMBASE-were searched on 20 December 2022, using specific search terms. Case-control, cohort, and randomized clinical trial studies comparing patients with FMF and healthy controls were considered eligible. We excluded systematic reviews, meta-analyses, case series with fewer than five cases, republished articles without new findings on pregnancy outcomes, studies targeting paternal FMF, and those not published in English. The results were summarized in the form of odds ratios (ORs) and 95% CIs, using a random-effects model. This study was registered in the University hospital Medical Information Network Clinical Trials Registry (Japan) as UMIN000049827. RESULTS: The initial electronic search identified 611 records, of which 9 were included in this meta-analysis (177 735 pregnancies, 1242 with FMF, and 176 493 healthy controls). FMF was significantly associated with increased odds of preterm deliveries (OR, 1.67; 95% CI, 1.05-2.67; I2 = 22%) and insignificantly associated with increased odds of fetal growth restriction (OR, 1.45; 95% CI, 0.90-2.34; I2 = 0%) and hypertensive disorders during pregnancy (OR, 1.28; 95% CI, 0.87-1.87; I2 = 0%). CONCLUSION: FMF was significantly associated with preterm delivery and insignificantly associated with fetal growth restriction and hypertensive disorders. All of the included studies were observational studies. Treatment characteristics were not fully collected from the articles, and further analysis of treatments for FMF in pregnancy is still warranted.


Assuntos
Febre Familiar do Mediterrâneo , Hipertensão Induzida pela Gravidez , Nascimento Prematuro , Gravidez , Recém-Nascido , Feminino , Humanos , Resultado da Gravidez , Retardo do Crescimento Fetal , Febre Familiar do Mediterrâneo/complicações , Febre Familiar do Mediterrâneo/tratamento farmacológico , Ensaios Clínicos Controlados Aleatórios como Assunto
2.
Biochem Biophys Res Commun ; 674: 147-153, 2023 09 24.
Artigo em Inglês | MEDLINE | ID: mdl-37419036

RESUMO

The age-related hearing loss allele (Cdh23ahl) of the cadherin 23 gene leads to a more severe hearing loss phenotype through additive effects with risk alleles for hearing loss. In this study, we genome edited the Cdh23ahl allele to the wild-type Cdh23+ allele in outbred ICR mice and inbred NOD/Shi mice established from ICR mice and investigated their effects on hearing phenotypes. Several hearing tests confirmed that ICR mice developed early onset high-frequency hearing loss and exhibited individual differences in hearing loss onset times. Severe loss of cochlear hair cells was also detected in the high-frequency areas in ICR mice. These phenotypes were rescued by genome editing the Cdh23ahl allele to Cdh23+, suggesting that abnormal hearing phenotypes develop because of the interaction of the Cdh23ahl and risk alleles in the genetic background of ICR mice. NOD/Shi mice developed more severe hearing loss and hair cell degeneration than ICR mice. Hearing loss was detected at 1 month old. Hair cell loss, including degeneration of cell bodies and stereocilia, was observed in all regions of the cochlea in NOD/Shi mice. Although these phenotypes were partially rescued by genome editing to the Cdh23+ allele, the phenotypes associated with high-frequency hearing were mostly unrecovered in NOD/Shi mice. These results strongly suggest that the genetic background of NOD/Shi mice contain a potential risk allele for the acceleration of early onset high-frequency hearing loss.


Assuntos
Surdez , Perda Auditiva de Alta Frequência , Camundongos , Animais , Alelos , Camundongos Endogâmicos NOD , Perda Auditiva de Alta Frequência/genética , Camundongos Endogâmicos ICR , Camundongos Endogâmicos C57BL , Surdez/genética , Caderinas/genética
3.
J Virol ; 96(15): e0056122, 2022 08 10.
Artigo em Inglês | MEDLINE | ID: mdl-35867561

RESUMO

Enterovirus A71 (EV-A71) is a human pathogen that causes hand, foot, and mouth disease, which can progress to severe neurological disease. EV-A71 infects humans via the human scavenger receptor B2 (hSCARB2). It can also infect neonatal mice experimentally. Wild-type (WT) EV-A71 strains replicate primarily in the muscle of neonatal mice; however, susceptibility lasts only for a week after birth. Mouse-adapted (MA) strains, which can be obtained by serial passages in neonatal mice, are capable of infecting both muscle and neurons of the central nervous system. It is not clear how the host range and tropism of EV-A71 are regulated and why neonatal mice lose their susceptibility during development. We hypothesized that EV-A71 infection in neonatal mice is mediated by mouse Scarb2 (mScarb2) protein. Rhabdomyosarcoma (RD) cells expressing mScarb2 were prepared. Both WT and MA strains infected mScarb2-expressing cells, but the infection efficiency of the WT strain was much lower than that of the MA strain. Infection by WT and MA strains in vivo was abolished completely in Scarb2-/- mice. Scarb2+/- mice, in which Scarb2 expression was approximately half of that in Scarb2+/+ mice, showed a milder pathology than Scarb2+/+ mice after infection with the WT strain. The Scarb2 expression level in muscle decreased with aging, which was consistent with the reduced susceptibility of aged mice to infection. These results indicated that EV-A71 infection is mediated by mScarb2 and that the severity of the disease, the spread of virus, and the susceptibility period are modulated by mScarb2 expression. IMPORTANCE EV-A71 infects humans naturally but can also infect neonatal mice. The tissue tropism and severity of EV-A71 disease are determined by several factors, among which the virus receptor is thought to be important. We show that EV-A71 can infect neonatal mice using mScarb2. However, the infection efficiency of WT strains via mScarb2 is so low that an elevated virus-receptor interaction associated with mouse adaptation mutation and decrease in mScarb2 expression level during development modulate the severity of the disease, the spread of virus, and the susceptibility period in the artificial neonatal mice model.


Assuntos
Antígenos CD36 , Enterovirus Humano A , Proteínas de Membrana Lisossomal , Receptores Virais , Animais , Animais Recém-Nascidos/metabolismo , Animais Recém-Nascidos/virologia , Antígenos CD36/biossíntese , Antígenos CD36/metabolismo , Modelos Animais de Doenças , Suscetibilidade a Doenças , Enterovirus Humano A/metabolismo , Enterovirus Humano A/patogenicidade , Doença de Mão, Pé e Boca/metabolismo , Doença de Mão, Pé e Boca/transmissão , Doença de Mão, Pé e Boca/virologia , Especificidade de Hospedeiro , Humanos , Proteínas de Membrana Lisossomal/biossíntese , Proteínas de Membrana Lisossomal/metabolismo , Camundongos , Receptores Virais/biossíntese , Receptores Virais/metabolismo , Tropismo Viral , Virulência
4.
FASEB J ; 36(5): e22301, 2022 05.
Artigo em Inglês | MEDLINE | ID: mdl-35478358

RESUMO

Psoriasis is an inflammatory disorder characterized by keratinocyte hyper-proliferation and Th17-type immune responses. However, the roles of bioactive lipids and the regulation of their biosynthesis in this chronic skin disease are not fully understood. Herein, we show that group IVE cytosolic phospholipase A2 (cPLA2 ε/PLA2G4E) plays a counterregulatory role against psoriatic inflammation by producing the anti-inflammatory lipid N-acylethanolamine (NAE). Lipidomics analysis of mouse skin revealed that NAE species and their precursors (N-acyl-phosphatidylethanolamine and glycerophospho-N-acylethanolamine) were robustly increased in parallel with the ongoing process of imiquimod (IMQ)-induced psoriasis, accompanied by a marked upregulation of cPLA2 ε in epidermal keratinocytes. Genetic deletion of cPLA2 ε exacerbated IMQ-induced ear swelling and psoriatic marker expression, with a dramatic reduction of NAE-related lipids in IMQ-treated, and even normal, skin. Stimulation of cultured human keratinocytes with psoriatic cytokines concomitantly increased PLA2G4E expression and NAE production, and supplementation with NAEs significantly attenuated the cytokine-induced upregulation of the psoriatic marker S100A9. Increased expression of cPLA2 ε was also evident in the epidermis of psoriatic patients. These findings reveal for the first time the in vivo role of cPLA2 ε, which is highly induced in the keratinocytes of the psoriatic skin, promotes the biosynthesis of NAE-related lipids, and contributes to limiting psoriatic inflammation.


Assuntos
Psoríase , Animais , Anti-Inflamatórios/uso terapêutico , Anticorpos , Citocinas/metabolismo , Etanolaminas , Humanos , Imiquimode , Inflamação , Lipídeos/efeitos adversos , Camundongos , Fosfolipases/uso terapêutico , Psoríase/induzido quimicamente , Psoríase/tratamento farmacológico
5.
Intern Med ; 59(2): 293-296, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31534081

RESUMO

Opioid-induced-constipation (OIC) can be treated by naldemedine and other peripherally acting mu-opioid receptor antagonists (PAMORA) via a novel mechanism. We describe the case of a 52-year-old female outpatient who developed OIC while receiving oxycodone for pain due to cancer with multiple bone metastases. Although she did not have brain metastasis, opioid withdrawal syndrome (OWS) developed after taking naldemedine orally. Her Clinical Opiate-Withdrawal Score (COWS) was 19 (moderate symptoms). However, she recovered from OWS on intravenous fentanyl and a continuous infusion of oxycodone. She did not develop OWS thereafter and was discharged two days after recovery.


Assuntos
Naltrexona/análogos & derivados , Antagonistas de Entorpecentes/efeitos adversos , Constipação Induzida por Opioides/tratamento farmacológico , Oxicodona/efeitos adversos , Síndrome de Abstinência a Substâncias/etiologia , Analgésicos Opioides/uso terapêutico , Neoplasias da Mama/patologia , Dor do Câncer/tratamento farmacológico , Feminino , Humanos , Pessoa de Meia-Idade , Naltrexona/efeitos adversos , Naltrexona/uso terapêutico , Antagonistas de Entorpecentes/uso terapêutico , Oxicodona/uso terapêutico , Síndrome de Abstinência a Substâncias/tratamento farmacológico
6.
Sci Rep ; 8(1): 425, 2018 01 11.
Artigo em Inglês | MEDLINE | ID: mdl-29323231

RESUMO

Accumulation of somatic mutations in mitochondrial DNA (mtDNA) has been proposed to be responsible for human aging and age-associated mitochondrial respiration defects. However, our previous findings suggested an alternative hypothesis of human aging-that epigenetic changes but not mutations regulate age-associated mitochondrial respiration defects, and that epigenetic downregulation of nuclear-coded genes responsible for mitochondrial translation [e.g., glycine C-acetyltransferase (GCAT), serine hydroxymethyltransferase 2 (SHMT2)] is related to age-associated respiration defects. To examine our hypothesis, here we generated mice deficient in Gcat or Shmt2 and investigated whether they have respiration defects and premature aging phenotypes. Gcat-deficient mice showed no macroscopic abnormalities including premature aging phenotypes for up to 9 months after birth. In contrast, Shmt2-deficient mice showed embryonic lethality after 13.5 days post coitum (dpc), and fibroblasts obtained from 12.5-dpc Shmt2-deficient embryos had respiration defects and retardation of cell growth. Because Shmt2 substantially controls production of N-formylmethionine-tRNA (fMet-tRNA) in mitochondria, its suppression would reduce mitochondrial translation, resulting in expression of the respiration defects in fibroblasts from Shmt2-deficient embryos. These findings support our hypothesis that age-associated respiration defects in fibroblasts of elderly humans are caused not by mtDNA mutations but by epigenetic regulation of nuclear genes including SHMT2.


Assuntos
Senilidade Prematura/genética , Epigênese Genética , Genes Letais , Glicina Hidroximetiltransferase/genética , Mitocôndrias/fisiologia , Acetiltransferases/deficiência , Acetiltransferases/genética , Animais , Células Cultivadas , Desenvolvimento Embrionário , Feminino , Fibroblastos/citologia , Fibroblastos/metabolismo , Técnicas de Inativação de Genes , Glicina Hidroximetiltransferase/deficiência , Humanos , Masculino , Camundongos , Mitocôndrias/genética , Modelos Animais , N-Formilmetionina/metabolismo , RNA de Transferência/genética
7.
Biochem Biophys Res Commun ; 496(1): 231-237, 2018 01 29.
Artigo em Inglês | MEDLINE | ID: mdl-29317205

RESUMO

The Foxe3rct mutation, which causes early-onset cataracts, is a recessive mutation found in SJL/J mice. A previous study reported that cataract phenotypes are modified by the genetic background of mouse inbred strains and that the Pde6brd1 mutation, which induced degeneration of the photoreceptor cells, is a strong candidate genetic modifier to accelerate the severity of cataractogenesis of Foxe3rct mice. We created congenic mice by transferring a genomic region including the Foxe3rct mutation to the B6 genetic background, which does not carry the Pde6brd1 mutation. In the congenic mice, the cataract phenotypes became remarkably mild, and the development of cataracts was suppressed for a long time. Moreover, we created transgenic mice by injecting BAC clones including the wild-type Pde6b gene into the eggs of SJL-Foxe3rct mice. Although the resistant effect for cataract phenotypes in transgenic mice was less than that in congenic mice, the severity and onset time of cataract phenotypes were clearly improved and delayed, respectively, compared with the phenotypes of the original SJL-Foxe3rct mice. These results clearly show that the development of early-onset cataracts requires at least two mutant alleles of Foxe3rct and Pde6brd1, and another modifier associated with the severity of cataract phenotypes in Foxe3rct mice underlies the genetic backgrounds in mice.


Assuntos
Catarata/genética , Catarata/metabolismo , Nucleotídeo Cíclico Fosfodiesterase do Tipo 6/genética , Nucleotídeo Cíclico Fosfodiesterase do Tipo 6/metabolismo , Fatores de Transcrição Forkhead/genética , Fatores de Transcrição Forkhead/metabolismo , Predisposição Genética para Doença/genética , Animais , Progressão da Doença , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Transgênicos , Relação Estrutura-Atividade
8.
Sci Rep ; 7(1): 11208, 2017 09 11.
Artigo em Inglês | MEDLINE | ID: mdl-28894263

RESUMO

Using a forward genetics approach to map loci in a mouse skin cancer model, we previously identified a genetic locus, Skin tumour modifier of MSM 1 (Stmm1) on chromosome 7, conferring strong tumour resistance. Sub-congenic mapping localized Parathyroid hormone (Pth) in Stmm1b. Here, we report that serum intact-PTH (iPTH) and a genetic polymorphism in Pth are important for skin tumour resistance. We identified higher iPTH levels in sera from cancer-resistant MSM/Ms mice compared with susceptible FVB/NJ mice. Therefore, we performed skin carcinogenesis experiments with MSM-BAC transgenic mice (Pth MSM-Tg) and Pth knockout heterozygous mice (Pth +/-). As a result, the higher amounts of iPTH in sera conferred stronger resistance to skin tumours. Furthermore, we found that the coding SNP (rs51104087, Val28Met) localizes in the mouse Pro-PTH encoding region, which is linked to processing efficacy and increased PTH secretion. Finally, we report that PTH increases intracellular calcium in keratinocytes and promotes their terminal differentiation. Taken together, our data suggest that Pth is one of the genes responsible for Stmm1, and serum iPTH could serve as a prevention marker of skin cancer and a target for new therapies.


Assuntos
Hormônios e Agentes Reguladores de Cálcio/genética , Hormônios e Agentes Reguladores de Cálcio/metabolismo , Predisposição Genética para Doença , Hormônio Paratireóideo/genética , Hormônio Paratireóideo/metabolismo , Neoplasias Cutâneas/epidemiologia , Neoplasias Cutâneas/genética , Animais , Modelos Animais de Doenças , Camundongos , Camundongos Knockout , Camundongos Transgênicos , Polimorfismo de Nucleotídeo Único
9.
Transgenic Res ; 26(4): 559-565, 2017 08.
Artigo em Inglês | MEDLINE | ID: mdl-28220277

RESUMO

Mitochondrial DNA segregation is one of the characteristic modes of mitochondrial inheritance in which the heteroplasmic state of mitochondrial DNA is transmitted to the next generation in variable proportions. To analyze mitochondrial DNA segregation, we produced a heteroplasmic mouse strain with interspecific mitochondrial DNA haplotypes, which contains two types of mitochondrial DNA derived from C57BL/6J and Mus spretus strains. The strain was produced on a C57BL/6J nuclear genomic background by microinjection of donor cytoplasm into fertilized eggs. The PCR-RFLP semi-quantitative analysis method, which was improved to reduce the effect of heteroduplex formation, was used to measure the proportion of heteroplasmic mitochondrial DNA in tissues. Founder mice contained up to approximately 14% of exogenous Mus spretus mitochondrial DNA molecules in their tails, and the detected proportions differed among tissues of the same individual. Heteroplasmic mitochondrial DNA is transmitted to the next generation in varying proportions under the maternal inheritance mode. This mitochondrial heteroplasmic mouse strain and the improved PCR-RFLP measurement system enable analysis of the transmission of heteroplasmic mitochondrial DNA variants between tissues and generations.


Assuntos
DNA Mitocondrial/genética , Haplótipos/genética , Polimorfismo de Fragmento de Restrição/genética , Animais , Feminino , Camundongos , Microinjeções , Reação em Cadeia da Polimerase , Zigoto/crescimento & desenvolvimento
10.
J Biol Chem ; 291(53): 27313-27322, 2016 12 30.
Artigo em Inglês | MEDLINE | ID: mdl-27881674

RESUMO

Calpains (CAPN) are a family of Ca2+-dependent cysteine proteases that regulate various cellular functions by cleaving diverse substrates. Of the 15 mammalian calpains, CAPN8 and CAPN9 are two that are expressed predominantly in the gastrointestinal tract, where they interact to form a protease complex, termed G-calpain. However, because native G-calpain exhibits a highly restricted expression pattern, it has never been purified, and the interactions between CAPN8 and CAPN9 have not been characterized. Here, we clarified the molecular nature of G-calpain by using recombinant proteins and transgenic mice expressing FLAG-tagged CAPN8 (CAPN8-FLAG). Recombinant mouse CAPN8 and CAPN9 co-expressed in eukaryotic expression systems exhibited the same mobility as native mouse G-calpain in Blue Native-PAGE gels, and CAPN8-FLAG immunoprecipitation from stomach homogenates of the transgenic mice showed that CAPN9 was the only protein that associated with CAPN8-FLAG. These results indicated that G-calpain is a heterodimer of CAPN8 and CAPN9. In addition, active recombinant G-calpain was expressed and purified using an in vitro translation system, and the purified protease exhibited enzymatic properties that were comparable with that of calpain-2. We found that an active-site mutant of CAPN8, but not CAPN9, compromised G-calpain's substrate cleavage activity, and that the N-terminal helix region of CAPN8 and the C-terminal EF-hands of CAPN8 and CAPN9 were involved in CAPN8/9 dimerization. Furthermore, CAPN8 protein in Capn9-/- mice was almost completely lost, whereas CAPN9 was only partially lost in Capn8-/- mice. Collectively, these results demonstrated that CAPN8 and CAPN9 function as catalytic and chaperone-like subunits, respectively, in G-calpain.


Assuntos
Calpaína/metabolismo , Mucosa Gástrica/metabolismo , Proteínas Recombinantes/metabolismo , Sequência de Aminoácidos , Animais , Catálise , Células Cultivadas , Feminino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Transgênicos , Isoformas de Proteínas , Homologia de Sequência de Aminoácidos
11.
Masui ; 63(3): 315-9, 2014 Mar.
Artigo em Japonês | MEDLINE | ID: mdl-24724442

RESUMO

BACKGROUND: We investigated whether sugammadex could reverse neuromuscular blockade induced by rocuronium in patients for cesarean delivery treated with magnesium sulfate preoperatively. METHODS: Twenty-three pregnant women received general anesthesia induced with thiopental and rocuronium. They were maintained by nitrous oxide, oxygen and sevoflurane (GOS) before delivery and after delivery by GOS, midazolam, and propofol. After the surgery, the patients with two or more counts of train-of-four (TOF), the moderate block group were classified into Mg (-) M and Mg (+) M, depending whether magnesium sulfate had been injected or not, and sugammadex 2 mg x kg(-1) was administered to both groups. Patients with PTC 2 or more, the profound block group, were classified into Mg (-) P or Mg (+) P and sugammadex 4 mg x kg(-1) was administered to both groups. Recovery time was defined as the time required to reach TOFR 0.9 or more after the injection of sugammadex. RESULTS: Median recovery times of the Mg (-) M, the Mg (-) P, the Mg (+) M and the Mg (+) P were 63 seconds (range: 26-130, N = 7), 127 seconds (range: 63-228, N = 7), 104 seconds (range: 67-133, N = 5), and 142 seconds (range: 57-209, N = 4), respectively. CONCLUSIONS: Sugammadex could reverse rocuronium-induced neuromuscular blockade in a dose-response manner even in the patients treated with magnesium sulfate.


Assuntos
Androstanóis/antagonistas & inibidores , Período de Recuperação da Anestesia , Anestesia Obstétrica , Cesárea , Sulfato de Magnésio/administração & dosagem , Relaxamento Muscular/efeitos dos fármacos , Bloqueio Neuromuscular , Fármacos Neuromusculares não Despolarizantes/antagonistas & inibidores , Assistência Perioperatória , gama-Ciclodextrinas/farmacologia , Adulto , Androstanóis/administração & dosagem , Androstanóis/farmacologia , Relação Dose-Resposta a Droga , Feminino , Humanos , Fármacos Neuromusculares não Despolarizantes/administração & dosagem , Fármacos Neuromusculares não Despolarizantes/farmacologia , Gravidez , Rocurônio , Sugammadex , gama-Ciclodextrinas/administração & dosagem
12.
Masui ; 63(3): 320-3, 2014 Mar.
Artigo em Japonês | MEDLINE | ID: mdl-24724443

RESUMO

BACKGROUND: Although magnesium can potentially shorten onset time of a muscle relaxant, it is unclear whether it does shorten the onset time of rocuronium for patients in cesarean delivery (CD). Thus the purpose of this study is to compare the onset time of rocuronium in patients with or without preoperative magnesium treatment. METHODS: In 9 CD patients without magnesium (CD group) and 8 CD patients with magnesium (CD-Mg group), neuromuscular function was continuously assessed with acceleromyography immediately after induction of general anesthesia with intravenous injection of thiopental (4-5 mg x kg(-1)) and rocuronium (0.6 mg x kg(-1)). Onset time of muscle relaxation, defined by the time from rocuronium bolus injection until complete absence of twitch height, was measured. RESULTS: The onset time of both groups did not significantly differ (50 +/- 10 seconds vs. 51 +/- 11 seconds; P = 0.93, unpaired t-test). Median magnesium concentration, 5.2 mg x dl(-1) (3.8-6.3), and the onset time of CD-Mg group did not correlate magnesium concentrations (P = 0.23, Pearson's correlation coefficient test). CONCLUSIONS: Magnesium did not shorten the onset time of rocuronium 0.6 mg x kg(-1) in CD patients.


Assuntos
Androstanóis/farmacologia , Cesárea , Compostos de Magnésio/farmacologia , Relaxamento Muscular/efeitos dos fármacos , Fármacos Neuromusculares não Despolarizantes/farmacologia , Adulto , Androstanóis/administração & dosagem , Anestesia Geral , Anestesia Obstétrica , Interações Medicamentosas , Feminino , Humanos , Compostos de Magnésio/administração & dosagem , Pessoa de Meia-Idade , Fármacos Neuromusculares não Despolarizantes/administração & dosagem , Gravidez , Cuidados Pré-Operatórios , Rocurônio , Fatores de Tempo
13.
Masui ; 63(3): 324-7, 2014 Mar.
Artigo em Japonês | MEDLINE | ID: mdl-24724444

RESUMO

BACKGROUND: Rapid onset of a muscle relaxant is required for general anesthesia with rapid sequence induction technique. Although a high dose of rocuronium was demonstrated to achieve the rapid onset in non-pregnant persons, it is still unknown whether this is true in women during late pregnancy. The purpose of this study is to compare the onset time of high dose rocuronium between pregnant and non-pregnant women. METHODS: Ten pregnant women undergoing cesarean delivery and 10 age, height, and body weight-matched non-pregnant women were enrolled in the study. Neuromuscular function was continuously assessed by twitch response with acceleromyography immediately after induction of general anesthesia with intravenous injection of thiopental (4-5 mg x kg(-1)) and rocuronium (0.9 mg x kg(-1)). Onset time of muscle relaxation, defined by the time from rocuronium bolus injection to complete absence of twitch height, was measured. RESULTS: The onset time of rocuronium in pregnant women was significantly shorter than that in non-pregnant women (42 +/- 9 seconds vs. 56 +/- 10 seconds; P = 0.0039. unpaired t-test). CONCLUSIONS: The onset time of high dose of rocuronium in pregnant women was significantly shorter than that of non-pregnant women.


Assuntos
Androstanóis/farmacologia , Anestesia Geral , Anestesia Obstétrica , Relaxamento Muscular/efeitos dos fármacos , Fármacos Neuromusculares não Despolarizantes/farmacologia , Gravidez/fisiologia , Adulto , Androstanóis/administração & dosagem , Cesárea , Feminino , Humanos , Fármacos Neuromusculares não Despolarizantes/administração & dosagem , Rocurônio , Fatores de Tempo , Adulto Jovem
14.
BMC Public Health ; 13: 754, 2013 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-23945173

RESUMO

BACKGROUND: The prevalence of chronic obstructive pulmonary disease (COPD) is similar in Japan and China and is increasing due to high rates of smoking in these countries. Reducing COPD is an important public health issue. The goals of this study were to verify the reliability and validity of the Japanese version of the COOP/WONCA charts, a tool for measuring health status, and to examine the qualitative differences in health status between Japanese and Chinese patients with COPD and between these patients and healthy subjects. METHODS: From 2008 to 2011, we examined the factors affecting the health status of Japanese and Chinese populations living in six cities. Participants were patients with COPD staged according to the Global Initiative for Chronic Obstructive Lung Disease (GOLD) criteria (140 Japanese, 201 Chinese) and healthy subjects (243 Japanese, 199 Chinese), all 50 to 79 years old. Health status was measured by using the COOP/WONCA charts, and basic information such as smoking status and medical history was reported by the participants. RESULTS: The Japanese and Chinese versions of the COOP/WONCA charts were shown to be reliable and valid by test-retest, comparison with the SF-36 and respiratory symptoms, and correlation of results obtained from patients and their physicians. Stepwise multiple regression analyses demonstrated that "Physical fitness", "Daily activities", and "Social activities" were predicted by COPD status and/or respiratory symptoms; "Feelings" by nationality and respiratory symptoms; "Pain" by sex and respiratory symptoms; and "Overall health" by nationality. When the COOP/WONCA scores were stratified by nationality, age, sex, and COPD status, the difference of each score between the patients and healthy subjects was larger for the Chinese subjects than for the Japanese. The physical, psychosocial activities, and pain scores increased significantly as COPD status worsened in Chinese subjects, whereas these scores were not affected by sex, age, or COPD status for Japanese subjects. Brinkman index and use of smoky fuel indoors affected the COOP/WONCA scores in Chinese patients but not in Japanese patients. CONCLUSIONS: The Japanese COOP/WONCA charts are reliable and valid. COPD more severely affected the health status of Chinese participants than of Japanese participants. These results suggest that countermeasures against insufficient health care and smoky environments may improve the health status of Chinese patients with COPD.


Assuntos
Nível de Saúde , Doença Pulmonar Obstrutiva Crônica/fisiopatologia , Inquéritos e Questionários , Idoso , China , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Pesquisa Qualitativa , Reprodutibilidade dos Testes
15.
Masui ; 62(4): 395-401, 2013 Apr.
Artigo em Japonês | MEDLINE | ID: mdl-23697188

RESUMO

BACKGROUND: It has been demonstrated that preparatory ultrasound scanning can facilitate lumbar epidural anesthesia. We compared longitudinal with transverse approach of ultrasound imaging to test the precision and the quality of acquired images in cesar ean delivery patients. METHODS: We enrolled 34 cesarean delivery patients. Measured distances from the skin to dura on each ultrasound view was compared with the actual needle depth. Additionally we measured ultrasound visibility score. RESULTS: The both distances measured in ultrasound strongly correlated with the actual depth (R2= 0.87 and 95% limits of agreement of -3.07 to 6.45 mm on longitudinal view, R2=0.82 and 95% limits of agreement of -3.50 to 7.74 mm on longitudinal view). The visibility score of the longitudinal view was significantly higher than that of transverse view (P<0.01). CONCLUSIONS: Both planes provided accurate information about the epidural space and depth, and the longitudinal plane was superior in the imaging quality. It was suggested that both approaches may help identify epidural spaces.


Assuntos
Anestesia Epidural/métodos , Anestesia Obstétrica/métodos , Cesárea , Espaço Epidural/diagnóstico por imagem , Adulto , Feminino , Humanos , Região Lombossacral , Gravidez , Ultrassonografia
16.
Diabetes Res Clin Pract ; 99(1): 39-47, 2013 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-23107110

RESUMO

AIMS: An appropriate questionnaire for assessing family support of self-management behavior of Japanese Type 2 diabetes patients has yet to be developed. We produced a Japanese version of the Diabetes Family Behavior Checklist (DFBC) and tested its reliability and validity. METHODS: The study enrolled Japanese patients with Type 2 diabetes mellitus who were living with their families: 158 patients in the Insulin Group and 169 in the Oral Hypoglycemic Agents Group. The external validity of the DFBC was tested with questionnaires of self-managed dietary and exercise behaviors, the Appraisal of Diabetes Scale (ADS), and HbA1c. RESULTS: The DFBC comprised two components: "Negative" and "Positive" feedbacks. Cronbach's alpha in the subcategories was ≥0.93, and the test-retest showed an intraclass correlation coefficient of 0.89. "Positive" and "Negative" scores correlated with self-managed dietary and/or exercise behaviors, the ADS scores in the both Groups. For patients having HbA1c levels of ≤6.8% there was a correlation between their "Positive" and "Negative" scores and the scores of their families in both Groups. CONCLUSION: The DFBC showed evidence of validity and reliability and may be a useful tool for quick assessment of self-managed treatment behavior of Japanese Type 2 diabetes patients and support received from their family.


Assuntos
Diabetes Mellitus Tipo 2/terapia , Família , Comportamentos Relacionados com a Saúde , Cooperação do Paciente , Autocuidado , Apoio Social , Idoso , Terapia Combinada , Estudos Transversais , Diabetes Mellitus Tipo 2/sangue , Diabetes Mellitus Tipo 2/dietoterapia , Diabetes Mellitus Tipo 2/tratamento farmacológico , Dieta para Diabéticos/etnologia , Exercício Físico , Família/etnologia , Feminino , Hemoglobinas Glicadas/análise , Comportamentos Relacionados com a Saúde/etnologia , Humanos , Hipoglicemiantes/administração & dosagem , Hipoglicemiantes/uso terapêutico , Japão , Masculino , Pessoa de Meia-Idade , Cooperação do Paciente/etnologia , Reprodutibilidade dos Testes , Inquéritos e Questionários
17.
Nature ; 476(7360): 332-5, 2011 Jul 31.
Artigo em Inglês | MEDLINE | ID: mdl-21804566

RESUMO

'Florigen' was proposed 75 years ago to be synthesized in the leaf and transported to the shoot apex, where it induces flowering. Only recently have genetic and biochemical studies established that florigen is encoded by FLOWERING LOCUS T (FT), a gene that is universally conserved in higher plants. Nonetheless, the exact function of florigen during floral induction remains poorly understood and receptors for florigen have not been identified. Here we show that the rice FT homologue Hd3a interacts with 14-3-3 proteins in the apical cells of shoots, yielding a complex that translocates to the nucleus and binds to the Oryza sativa (Os)FD1 transcription factor, a rice homologue of Arabidopsis thaliana FD. The resultant ternary 'florigen activation complex' (FAC) induces transcription of OsMADS15, a homologue of A. thaliana APETALA1 (AP1), which leads to flowering. We have determined the 2.4 Å crystal structure of rice FAC, which provides a mechanistic basis for florigen function in flowering. Our results indicate that 14-3-3 proteins act as intracellular receptors for florigen in shoot apical cells, and offer new approaches to manipulate flowering in various crops and trees.


Assuntos
Proteínas 14-3-3/metabolismo , Proteínas de Arabidopsis , Flores/crescimento & desenvolvimento , Flores/metabolismo , Oryza/metabolismo , Proteínas de Plantas/metabolismo , Proteínas de Arabidopsis/química , Proteínas de Ligação ao Cálcio/química , Núcleo Celular/metabolismo , Regulação da Expressão Gênica de Plantas , Proteínas de Domínio MADS/química , Modelos Moleculares , Complexos Multiproteicos/química , Complexos Multiproteicos/metabolismo , Oryza/genética , Oryza/crescimento & desenvolvimento , Proteínas de Plantas/química , Proteínas de Plantas/genética , Brotos de Planta/citologia , Ligação Proteica , Homologia de Sequência de Aminoácidos , Fatores de Transcrição/química , Técnicas do Sistema de Duplo-Híbrido
18.
Masui ; 60(7): 790-8, 2011 Jul.
Artigo em Japonês | MEDLINE | ID: mdl-21800657

RESUMO

Many anesthesiologists are reluctant to depart from their traditional long fasting periods, even though many guidelines recommend that oral intake of clear fluids administered up to 2-3 hours prior to general anesthesia does not adversely affect the gastric contents. It also indicates that the application of these guidelines does not affect the incidence of pulmonary aspiration. One of the reasons why they have not changed their practices is that they wonder whether it is safe to administer clear fluids as recommended in the guidelines. In this review, we emphasize that oral rehydration therapy using clear fluids (such as OS-1, water and carbohydrate-rich beverage) is safe based on the non-invasive gastric echo examinations as many guidelines have already indicated. Oral rehydration therapy should be considered not only as an alternative to intravenous therapy for preoperative fluid and electrolyte management but also as one of the important modalities which can enhance the recovery of surgical patients.


Assuntos
Carboidratos/administração & dosagem , Hidratação , Cuidados Pré-Operatórios , Recuperação de Função Fisiológica , Soluções para Reidratação/administração & dosagem , Segurança , Estômago/diagnóstico por imagem , Adulto , Anestesia Geral , Conteúdo Gastrointestinal , Humanos , Masculino , Pessoa de Meia-Idade , Período Pós-Operatório , Guias de Prática Clínica como Assunto , Procedimentos Cirúrgicos Operatórios , Fatores de Tempo , Ultrassonografia
19.
Masui ; 60(5): 615-20, 2011 May.
Artigo em Japonês | MEDLINE | ID: mdl-21626867

RESUMO

BACKGROUND: OS-1 is an oral rehydration solution that conforms with the principles of oral rehydration therapy. It may be useful for preoperative fluid management of surgical patients. While intake of clear fluids 2 hours before surgery is considered safe, it is not known if the same applies to OS-1. We therefore investigated the safety of OS-1 for preoperative patients as compared with clear fluids. METHODS: First, eight healthy adult volunteers were studied in a crossover manner. Volunteers ingested 500 ml of OS-1 or water (clear fluid). Gastric emptying time was measured using gastric ultrasonography. Gastric antral area as measured by ultrasonography correlates well with gastric volume in a close-to-linear manner. Next, we measured gastric volume of elective surgical patients who had drunk OS-1 until two hours before the induction of anesthesia. RESULTS: Gastric emptying time did not differ between OS-1 and water. The stomach was emptied 30 minutes after ingestion of both OS-1 and water. The fasting stomach was identified in all patients who had drunk OS-1 before surgery. CONCLUSIONS: We concluded that allowing elective surgical patients to drink OS-1 until two hours before anesthesia did not affect the volume of gastric contents.


Assuntos
Hidratação , Cuidados Pré-Operatórios , Segurança , Adulto , Idoso , Feminino , Esvaziamento Gástrico , Humanos , Masculino , Fatores de Tempo
20.
Masui ; 60(12): 1394-7, 2011 Dec.
Artigo em Japonês | MEDLINE | ID: mdl-22256582

RESUMO

A 32-year-old pregnant woman diagnosed with aplastic anemia was admitted for emergent caesarean delivery of 26th week of the gestation due to PIH (pregnancy-induced hypertension) and NRFS (non-reassuring fetal status). After compensating platelets counts to 5.3x10(4) microl-1, general anesthesia was induced with propofol and rocuronium. Anesthesia was maintained with O2 and sevoflurane until delivery and with modified-NLA after delivery. She was additionally monitored with Vigileo/FloTrac system (Edwards Lifesciences, USA) and TOF-WATCH SX (Nihon Kohden, Tokyo). After 8 minutes of operation her baby was born with the 5-minute Apgar score of 5 and the UA-pH of 7.387. It was only 2 hours and 12 minutes that the baby was born after she was admitted. The baby was tracheally intubated and transferred to NICU. Blood loss during operation was 835 g and two units of RCC was transfused. Circulatory values were kept acceptable and neuromuscular blocking was completely reversed by sugammadex and extubated in the operating room. Bleeding tendency and atonic bleeding were not observed. She survived perioperative period and was to be treated for aplastic anemia. Her baby was discharged neurologically free. We should be ready to respond to anesthetic requirement for urgent cases of aplastic anemia.


Assuntos
Anemia Aplástica/complicações , Anestesia Geral , Anestesia Obstétrica , Cesárea , Hipertensão Induzida pela Gravidez/etiologia , Complicações Hematológicas na Gravidez , Adulto , Anemia Aplástica/diagnóstico , Anemia Aplástica/terapia , Cuidados Críticos , Emergências , Feminino , Sofrimento Fetal/etiologia , Humanos , Recém-Nascido , Unidades de Terapia Intensiva Neonatal , Monitorização Intraoperatória , Assistência Perioperatória , Gravidez
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