T6SS nuclease effectors in Pseudomonas syringae act as potent antimicrobials in interbacterial competition.

Type VI secretion system (T6SS) is a potent weapon employed by various Pseudomonas species to compete with neighboring microorganisms for limited nutrients and ecological niches. However, the involvement of T6SS effectors in interbacterial competition within the phytopathogen Pseudomonas syringae remains unknown. In this study, we examined two T6SS clusters in a wild-type P. syringae MB03 and verified the involvement of one cluster, namely, T6SS-1, in interbacterial competition. Additionally, our results showed that two T6SS DNase effectors, specifically Tde1 and Tde4, effectively outcompeted antagonistic bacteria, with Tde4 playing a prominent role. Furthermore, we found several cognate immunity proteins, including Tde1ia, Tde1ib, and Tde4i, which are located in the downstream loci of their corresponding effector protein genes and worked synergistically to protect MB03 cells from self-intoxication. Moreover, expression of either Tde1 or C-terminus of Tde4 in Escherichia coli cells induced DNA degradation and changes in cell morphology. Thus, our results provide new insights into the role of the T6SS effectors of P. syringae in the interbacterial competition in the natural environment. IMPORTANCE: The phytopathogen Pseudomonas syringae employs an active type VI secretion system (T6SS) to outcompete other microorganisms in the natural environment, particularly during the epiphytic growth in the phyllosphere. By examining two T6SS clusters in P. syringae MB03, T6SS-1 is found to be effective in killing Escherichia coli cells. We highlight the excellent antibacterial effect of two T6SS DNase effectors, namely, Tde1 and Tde4. Both of them function as nuclease effectors, leading to DNA degradation and cell filamentation in prey cells, ultimately resulting in cell death. Our findings deepen our understanding of the T6SS effector repertoires used in P. syringae and will facilitate the development of effective antibacterial strategies.

The immobilization of cadmium by rape straw derived biochar in alkaline conditions: Sorption isotherm, molecular binding mechanism, and in-situ remediation of Cd-contaminated soil.

The issue of cadmium (Cd) contamination in alkaline soils is escalating, necessitating the prompt implementation of effective passivation strategies. Biochar has gained significant attention for its potential in immobilizing heavy metals; however, the suitability of biochar as a remediation material and its micro-scale interaction mechanisms with Cd under alkaline conditions remain unclear. Rape straw (RS) were pyrolyzed at 400 °C (RB400) and 700 °C (RB700) to produce biochar. Adsorption and soil incubation experiments were carried out to assess the feasibility of using rape straw derived biochar pyrolyze at different temperatures and understanding their remediation mechanisms in alkaline environments. The sorption capacity for Cd immobilization was evaluated using sorption isotherms, revealing that RB700 exhibited enhanced Cd sorption performance with a maximum sorption capacity of 119.33 mg g-1 calculated from the Langmuir isotherm equation at pH 8. Cd L3-edge X-ray absorption near-edge structure (XANES) spectroscopy analysis confirmed that the dominant sorption species of Cd were organic Cd in RB400, with CdCO3 precipitation increased to 73.9% in RB700. Solid-state 13C nuclear magnetic resonance (13C-NMR) spectroscopy demonstrated that aromatic and carboxyl C functional groups are involved in the organic sorption of Cd through complexation and Cd2+-π interactions in alkaline solutions. The precipitation of CdCO3 in RB700 may resulted in a more effective passivation effect compared to RB400, leading to a significant 15.54% reduction in the DTPA-Cd content in Cd-contaminated soil. These findings highlight the effective Cd passivation Cd in alkaline environments by rape straw derived biochar, providing new molecular insights into the Cd retention mechanism of biochar. Furthermore, it presents novel ideas for improving remediation approaches for alkaline Cd-contaminated soils.

Neonicotinoid insecticides in waters of the northern Jiangsu segment of the Beijing-Hangzhou Grand Canal: Environmental and health implications.

Neonicotinoid (NEO) insecticides have been frequently detected in natural aquatic environments. Nevertheless, the distribution of NEOs in artificial environments is not clear. The Beijing-Hangzhou Grand Canal is the longest canal in the world. The northern Jiangsu segment of the Grand Canal was selected to study the spatiotemporal variation and source of eight NEOs in the canal water and assess their ecological and health risks. The total NEO concentration in the canal water was 12-289 ng L-1 in the dry season and 18-373 ng L-1 in the wet season, which were within the concentration range in other 11 natural rivers worldwide. The average total NEO concentrations were not statistically different between the seasons; only the concentrations of imidaclothiz, thiacloprid (THI), acetamiprid, and dinotefuran were different. At city scale, the total NEO concentration in the dry season showed a decreasing trend along the water flow from Xuzhou City to Yangzhou City. The total NEO concentrations were found to be positively correlated with the sown area of farm crops and the rural labour force, indicating the agricultural influence on the spatial distribution of NEO concentrations. In the wet season, relatively high NEO concentrations were distributed in downstream sites under the influence of artificial regulation. The primary contributor to the NEO inputs into the canal was the nonpoint source in the dry and wet seasons, with a relative contribution of 68 %. THI, imidacloprid, clothianidin and thiamethoxan would produce chronic ecological risks in both seasons. Further consideration needs to be given to the above four NEOs and NEO mixtures. The human health risks that NEOs posed by drinking water were assessed based on the chronic daily intake (CDI). The maximum CDI for adults and children was lower than the reference doses. This suggested public health would not be at risk from canal water consumption.

Cyano-Rich g-C3 N4 in Photochemistry: Design, Applications, and Prospects.

Cyano-rich g-C3 N4 materials are widely used in various fields of photochemistry due to the very powerful electron-absorbing ability and electron storage function of cyano, as well as its advantages in improving light absorption, adjusting the energy band structure, increasing the polarization rate and electron density in the structure, active site concentration, and promoting oxygen activation ability. Notwithstanding, there is yet a huge knowledge break in the design, preparation, detection, application, and prospect of cyano-rich g-C3 N4 . Accordingly, an overall review is arranged to substantially comprehend the research progress and position of cyano-rich g-C3 N4 materials. An overall overview of the current research position in the synthesis, characterization (determination of their location and quantity), application, and reaction mechanism analysis of cyano-rich g-C3 N4 materials to provide a quantity of novel suggestions for cyano-modified carbon nitride materials' construction is provided. In view of the prevailing challenges and outlooks of cyano-rich g-C3 N4 materials, this paper will purify the growth direction of cyano-rich g-C3 N4 , to achieve a more in-depth exploration and broaden the applications of cyano-rich g-C3 N4 .

Synthesis and Properties of Carbon Microspheres from Waste Office Paper.

As a kind of biomass resource, waste office paper can be used as a carbon precursor to prepare carbon materials. In this work, carbon microspheres with regular shape, uniform particle size and high carbon content were successfully prepared from waste office paper via a hydrothermal synthesis method with sulfuric acid as the catalyst. The effects of reaction temperature and sulfuric acid dosage on the morphology of the carbon microspheres were studied. The formation mechanism of the carbon microspheres was investigated by analyzing the structure and composition of the products. The results show that the hydrolysis of cellulose in waste paper under hydrothermal conditions was the key for the formation of carbon microspheres. The temperature of hydrothermal reaction and the use of sulfuric acid can affect the morphology of carbon microspheres. The carbon microspheres synthesized at 210 °C with 10 mL sulfuric acid have the best surface morphology, with uniform particle size and higher dispersion. Cyclic voltammetry and electrochemical impedance spectroscopy show that the carbon microspheres have good capacitance performance and can be used in capacitors. This study provides a low-cost precursor for carbon microspheres as well as a new method for the recycle of waste paper.

The interaction of different chlorine-based additives with swine manure during pyrolysis: Effects on biochar properties and heavy metal volatilization.

Poor properties and high concentrations of heavy metals are still major concerns of successful application of animal manure-derived biochar into the environment. This work thus proposed to add chlorine-based additives (Cl-additives, i.e., CaCl2, MgCl2, KCl, NaCl, and PVC, 50 g Cl/ kg) to improve biochar properties and enhance heavy metal volatilization during swine manure pyrolysis. The results showed that the addition of CaCl2 could improve the retention of carbon (C) by up to 13.1% during pyrolysis, whereas other Cl-additives had little effect on it. Moreover, CaCl2 could enhance the aromaticity of biochar, as indicated by lower H/C ratio than raw biochar. Pretreatment with CaCl2, MgCl2 and PVC reduced phosphorus (P) solubility but increased its bioavailability via the formation of chlorapatite (Ca5(PO4)3Cl). The CaCl2 was more effective for enhancing the volatilization efficiency of heavy metals than other Cl-additives, except for Pb that tended to react with the generated Ca5(PO4)3Cl to form more stable and less volatile Pb5(PO4)3Cl. However, high pyrolysis temperature (900℃) was essential for CaCl2 to simultaneously decrease the bioavailability of heavy metals. Our results indicated that co-pyrolysis of swine manure with CaCl2 is a promising strategy to increase C retention, P bioavailability, and volatilization of heavy metals, and, at higher temperature, reduce the bioavailability of biochar-born heavy metals.

Occurrence, source, and risk assessment of neonicotinoid insecticides in the Huai River, China.

Neonicotinoids (NEOs) are the most widely used insecticides in the world. Nevertheless, the occurrence and distribution of NEOs in agricultural areas are not well understood. This study investigated the concentration, sources, ecological risks, and health risks of eight NEOs in the water of the Huai River, which flows through a typical agricultural area in China. The total concentration of NEOs in the river water ranged from 1.02 to 191.2 ng L-1, with an average of 64.1 ng L-1. Thiamethoxam was the dominant compound, with an average relative contribution of 42.5%. The average concentration of the total NEOs in downstream was significantly higher than that in upstream (p < 0.05). This may be related to the intensity of agricultural activities. The riverine NEO fluxes increased by approximately 12 times from the upper site to the lower site. More than 1.3 tons of NEOs in 2022 were transferred into Lake Hongze, the largest regulative lake on the Eastern Route of the South-to-North Water Diversion Project. Nonpoint sources were the major contributor to the total NEO inputs, and water use was the main output pathway. The risk assessment indicated that the individual NEOs in the river water presented low ecological risks. The NEO mixtures would produce chronic risks to aquatic invertebrates in 50% of the sampling sites, which were mostly distributed in downstream. Thus, more attention should be given to the downstream. Based on the Monte Carlo simulation, the health risks of NEOs via water consumption were estimated. The maximum chronic daily intakes were 8.4 × 10-4, 2.25 × 10-4, 1.27 × 10-4, 1.88 × 10-4 mg kg-1 day-1 for boys, girls, men, and women, respectively, which were approximately 2 orders of magnitude lower than the acceptable daily intake. Therefore, river water consumption would not be a concern for the public health.

Dexmedetomidine promotes necroptosis by upregulating PARP1 in non-small cell lung cancer.

The score and prognostic value of necroptosis were analyzed in the TCGA and GSE120622 datasets. Necroptosis has the highest correlation with the immune microenvironment, and the high score in NSCLC correlates with poor prognosis. Differentially expressed genes between non-small cell lung cancer (NSCLC) and controls in both datasets were identified and subjected to construct co-expression networks, respectively. Black and blue modules were selected because of high correction with necroptosis. The intersected two module genes were mainly involved in immune and inflammatory response, cell cycle process and DNA replication. Nine marker genes of necroptosis were identified in these modules and considered as candidate genes. Based on candidate genes, we identified two clusters utilizing concordance clustering, additionally dividing NSCLC samples into high- and low-risk groups. There were significant differences in overall survival between two clusters and between high- and low-risk groups. Furthermore, PARP1 was found among the candidate genes to be the target gene of dexmedetomidine acting on necroptosis. Molecular experimental results found that PARP1 was highly expressed in the dexmedetomidine treated NSCLC compared with the NSCLC. Candidate genes associated with necroptosis may provide a powerful prognostic tool for precision oncology. Dexmedetomidine may target PARP1 to promote necroptosis and then affect NSCLC.

Combined analysis of gut microbiome and serum metabolomics reveals novel biomarkers in patients with early-stage non-small cell lung cancer.

Non-small cell lung cancer (NSCLC) is the predominant form of lung cancer and is one of the most fatal cancers worldwide. Recently, the International Association for the Study of Lung Cancer (IASLC) proposed a novel grading system based on the predominant and high-grade histological patterns for invasive pulmonary adenocarcinoma (IPA). To improve outcomes for NSCLC patients, we combined serum metabolomics and fecal microbiology to screen biomarkers in patients with early-stage NSCLC and identified characteristic microbial profiles in patients with different grades of IPA. 26 genera and 123 metabolites were significantly altered in the early-stage NSCLC patients. Agathobacter, Blautia, Clostridium, and Muribaculacea were more abundant in the early-stage NSCLC patients compared with healthy controls. For the different grades of IPA, the characteristic microorganisms are as follows: Blautia and Marinobacter in IPA grade type 1; Dorea in IPA grade type 2; and Agathobacter in IPA grade type 3. In the metabolome results, the early-stage NSCLC group mainly included higher levels of sphingolipids (D-erythro-sphingosine 1-phosphate, palmitoyl sphingomyelin), fatty acyl (Avocadyne 1-acetate, 12(S)-HETE, 20-Carboxy-Leukotriene B4, Thromboxane B3, 6-Keto-prostaglandin f1alpha, Sebacic acid, Tetradecanedioic acid) and glycerophospholipids (LPC 20:2, LPC 18:0, LPC 18:4, LPE 20:2, LPC 20:1, LPC 16:1, LPC 20:0, LPA 18:2, LPC 17:1, LPC 17:2, LPC 19:0). Dysregulation of pathways, such as sphingolipid metabolism and sphingolipid signaling pathway may become an emerging therapeutic strategy for early-NSCLC. Correlation analysis showed that gut microbiota and serum metabolic profiles were closely related, while Muribaculacea and Clostridium were the core genera. These findings provide new biomarkers for the diagnosis of early-stage NSCLC and the precise grading assessment of prognostic-related IPAs, which are of clinical importance and warrant further investigation of the underlying molecular mechanisms.

Green synthesized hydroxyapatite for efficient immobilization of cadmium in weakly alkaline environment.

The development of cost-effective passivators for the remediation of heavy metal-contaminated soils has been a research hotspot and an unsolved challenge. Herein, a novel hydroxyapatite (GSCH) was synthesized by co-precipitating distiller effluent-derived Ca with (NH4)2HPO4 using straw-derived dissolved organic matter (S-DOM) as the dispersant. Batch adsorption experiments and soil incubation tests were performed to assess the immobilization efficiency of GSCH for Cd in weakly alkaline environments. As a result, GSCH showed an excellent adsorption efficiency to Cd with a maximum adsorption amount of ∼222 mg g-1, which was fairly competitive compared to other similar previously materials reported. The kinetic data indicated that the adsorption of Cd on GSCH was a chemical and irreversible process, while the thermodynamic data revealed a spontaneous (ΔG° < 0) and endothermic (ΔH° > 0) adsorption process. Based on mechanism analysis, both physisorption (e.g., electrostatic attraction and pore filling) and chemisorption (e.g., ion exchange and complexation) were responsible for Cd adsorption on GSCH. Particularly, the incorporated S-DOM and hydroxyapatite phase in GSCH acted synergistically in the adsorption process. The incubation results showed that GSCH application could significantly reduce the bioavailability, phytoavailability and bioaccessibility of Cd in soil by 48.4%-57.8%, 20.4%-28.6% and 12.6%-24.0%, respectively. Moreover, GSCH application also improved soil bacterial communities and enhanced soil nutrient availability. Overall, this is the first study to demonstrate the potential application value of GSCH in Cd immobilization, providing promising insights into the development of green and cost-effective hydroxyapatite-based passivators for the remediation of heavy metal-contaminated soils.

Ultrahigh sorption of sulfamethoxazole by potassium hydroxide-modified biochars derived from bean-worm skin waste.

Food processing of bean worm generates copious amount of skin as solid waste posing a serious environmental concern. The present study utilized bean worm skin (BWS) waste to produce KOH-modified biochars (KBWS-BCs) for the removal of sulfamethoxazole (SMX) from aqueous solution for the first time. Characterization of KBWS-BCs was systematically investigated via multiple instrumental analysis techniques. The sorption performance of KBWS-BCs as a function of solution pH, reaction time, initial SMX concentration, and reaction temperature was investigated using batch experiments. The classic kinetics and isotherm models were employed to fit the sorption data. KBWS-BCs exhibited large surface areas (3331-4742 m2 g-1) and ultrahigh sorption performance for SMX (maximum adsorption capacities of 909-2000 mg g-1), which were comparable to those of other modified biochars and even those of well-designed materials. Thermodynamic study indicated that the sorption of SMX on KBWS-BCs was a spontaneous (△G° < 0) and exothermic (△H° < 0) process. Mechanism analysis showed that both chemisorption and physisorption were responsible for the adsorption of SMX by KBWS-BCs. Overall, recycling BWS for preparation of high-performance biochars can be a "win-win" strategy for both disposal of BWS and removal of SMX from wastewater.

Structure and functional group regulation of plastics for efficient ammonia capture.

Activated carbon and metal organic frameworks have been tested as NH3 recovery adsorbents, however, they are limited due to low NH3 adsorption capacity and high cost, respectively. In this study, ethylene glycol dimethacrylate (EGDMA) polymers as the representative ester plastics were tested, and their structure and adsorption sites were regulated using HNO3, HCl, or H2SO4 with varied H+ concentrations. The results showed that the EGDMA polymers all used hydrolysis which promoted NH3 adsorption via different mechanisms. With HNO3 and HCl optimization, an increased surface area promoted NH3 adsorption via physical forces. H2SO4 optimization resulted in -COOH, -OH, and -SO3H formation, which reacted with NH3 by chemical adsorption and hydrogen bonds. This significantly increased the NH3 adsorption capacity (85.99 mg·g-1) compared to the material before optimization (0.36 mg·g-1). This study presents a novel low-cost and efficient method to recycle waste plastics as NH3 adsorbents.

Generation of a human iPSC line CIBi011-A from amniocytes of a healthy fetus.

Human induced pluripotent stem cells (iPSC) resemble human embryonic stem cells with potential to differentiate into cells of all adult tissues. Nonetheless human iPSCs may have an epigenetic memory of their donor tissue origin, are easier to differentiate to those lineages, and their potential to other cell fates can be controlled. We generated a human iPSC line CIBi011-A from amniocytes of a healthy fetus. CIBi011-A serves as a useful source to investigate the epigenetic memory of iPSCs. As an iPSC line from a healthy donor, this line can also serve as a potential cell source from which to develop stem cell-based cell therapies.

Surgical Trauma-induced CCL2 Upregulation Mediates Lung Cancer Progression by Promoting Treg Recruitment in Mice and Patients.

Surgical removal of the tumor is currently the first-line treatment for lung cancer, but the procedure may accelerate cancer progression through immunosuppression. However, whether CCL2 (C-C motif chemokine ligand 2) enhances cancer progression by affecting regulatory T cells (Tregs) remains unknown. We found that the volume and weight of tumors were larger in the surgical trauma group than in the control group. CCL2 expression and Treg abundance were increased in tumor tissues after surgical trauma, and CCL2 expression was positively associated with Treg abundance. These results demonstrated that surgical trauma contributes to lung cancer progression by increasing CCL2 expression, thus promoting Treg recruitment.

Indoor Particulate Matter in Urban Households: Sources, Pathways, Characteristics, Health Effects, and Exposure Mitigation.

Particulate matter (PM) is a complex mixture of solid particles and liquid droplets suspended in the air with varying size, shape, and chemical composition which intensifies significant concern due to severe health effects. Based on the well-established human health effects of outdoor PM, health-based standards for outdoor air have been promoted (e.g., the National Ambient Air Quality Standards formulated by the U.S.). Due to the exchange of indoor and outdoor air, the chemical composition of indoor particulate matter is related to the sources and components of outdoor PM. However, PM in the indoor environment has the potential to exceed outdoor PM levels. Indoor PM includes particles of outdoor origin that drift indoors and particles that originate from indoor activities, which include cooking, fireplaces, smoking, fuel combustion for heating, human activities, and burning incense. Indoor PM can be enriched with inorganic and organic contaminants, including toxic heavy metals and carcinogenic volatile organic compounds. As a potential health hazard, indoor exposure to PM has received increased attention in recent years because people spend most of their time indoors. In addition, as the quantity, quality, and scope of the research have expanded, it is necessary to conduct a systematic review of indoor PM. This review discusses the sources, pathways, characteristics, health effects, and exposure mitigation of indoor PM. Practical solutions and steps to reduce exposure to indoor PM are also discussed.

Ten Years of EWAS.

Epigenome-wide association study (EWAS) has been applied to analyze DNA methylation variation in complex diseases for a decade, and epigenome as a research target has gradually become a hot topic of current studies. The DNA methylation microarrays, next-generation, and third-generation sequencing technologies have prepared a high-quality platform for EWAS. Here, the progress of EWAS research is reviewed, its contributions to clinical applications, and mainly describe the achievements of four typical diseases. Finally, the challenges encountered by EWAS and make bold predictions for its future development are presented.

Distribution, behaviour, bioavailability and remediation of poly- and per-fluoroalkyl substances (PFAS) in solid biowastes and biowaste-treated soil.

Aqueous film-forming foam, used in firefighting, and biowastes, including biosolids, animal and poultry manures, and composts, provide a major source of poly- and perfluoroalkyl substances (PFAS) input to soil. Large amounts of biowastes are added to soil as a source of nutrients and carbon. They also are added as soil amendments to improve soil health and crop productivity. Plant uptake of PFAS through soil application of biowastes is a pathway for animal and human exposure to PFAS. The complexity of PFAS mixtures, and their chemical and thermal stability, make remediation of PFAS in both solid and aqueous matrices challenging. Remediation of PFAS in biowastes, as well as soils treated with these biowastes, can be achieved through preventing and decreasing the concentration of PFAS in biowaste sources (i.e., prevention through source control), mobilization of PFAS in contaminated soil and subsequent removal through leaching (i.e., soil washing) and plant uptake (i.e., phytoremediation), sorption of PFAS, thereby decreasing their mobility and bioavailability (i.e., immobilization), and complete removal through thermal and chemical oxidation (i.e., destruction). In this review, the distribution, bioavailability, and remediation of PFAS in soil receiving solid biowastes, which include biosolids, composts, and manure, are presented.

MiR-139-5p Targetedly Regulates YAF2 and Mediates the AKT/P38 MAPK Signaling Pathway to Alleviate the Metastasis of Non-Small Cell Lung Cancer Cells and Their Resistance Against Cisplatin.

OBJECTIVE: To explore relevant mechanisms of miR-139-5p in alleviating the metastasis of non-small cell lung cancer cells (NSCLC) and their resistance against cisplatin. METHODS: Quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot (WB) assays were carried out to determine the protein levels of miR-139-5p and YAF2, and cisplatin (DDP)-resistant NSCLC cell strains were established. Subsequently, an MTT assay was employed to evaluate the viability of the cell strains, a Transwell assay to evaluate cell invasion activity, and flow cytometry to analyze cell apoptosis rate. Finally, a Western blot assay was carried out to determine the protein levels of P-PI3K and p-p38. RESULTS: NSCLC tissues showed lower miR-139-5p expression and higher YAF2 expression than paracancerous tissues and human normal lung epithelial cells, and miR-139-5p was related to the prognosis of NSCLC patients. Overexpression of miR-139-5p or knock-down of YAF2 inhibited the proliferation and invasion of NSCLC cells and induced their apoptosis. Additionally, the dual-luciferase reporter assay verified a targeting relationship between miR-139-5p and YAF2. Overexpression of miR-139-5p and knockdown of YAF2 reversed the resistance of A549/DDP cells against DDP, inactivated p38 and Akt proteins, and inhibited the AKT/p38 MAPK signaling pathway. Furthermore, inhibiting the AKT/p38 MAPK signaling pathway with MK2206 resisted the effects of knock-down of miR-139-5p on DDP resistance in NSCLC cells. CONCLUSION: MiR-139-5p targetedly regulates YAF2 and mediates the AKT/p38 MAPK signaling pathway to alleviate the metastasis of NSCLC cells and their resistance against cisplatin, which may be a novel target for improving the therapeutic effect on NSCLC.