RESUMO
Osteoarthritis (OA) is a slowly progressive joint disease typically seen in middle-age to elderly people. At present, there is no ideal agent to treat OA. Chenodeoxycholic acid (CDCA) was a principal active constituent from animal bile. However, the therapeutic effect of CDCA on OA severity was largely unknown. The purpose of this study was to evaluate the therapeutic effect of intra-articular injection of CDCA in a rabbit OA model. OA was induced in experimental rabbits by anterior cruciate ligament transection (ACLT) and then rabbits were intra-articularly injected with CDCA (10 mg/kg or 50 mg/kg) once per week for 5 weeks. The results showed that CDCA significantly decreased cartilage degradation on the surface of femoral condyles, reducing the pathological changes of articular cartilage and synovial membrane by macroscopic and histological analysis. CDCA also significantly decreased bone destruction and erosion of joint evaluated by micro-CT. Furthermore, CDCA could markedly reduce the release of matrix metalloproteinase-1 (MMP-1), matrix metalloproteinase-3 (MMP-3), interleukin-1ß (IL-1ß), and prostaglandin E2 (PGE2) in synovial fluid. These observations highlight CDCA might be a potential therapeutic agent for OA.
Assuntos
Ácido Quenodesoxicólico/uso terapêutico , Osteoartrite/tratamento farmacológico , Animais , Ligamento Cruzado Anterior/cirurgia , Cartilagem Articular/patologia , Modelos Animais de Doenças , Ensaio de Imunoadsorção Enzimática , Fêmur/patologia , Injeções Intra-Articulares , Interleucina-1beta/metabolismo , Masculino , Metaloproteinase 1 da Matriz/metabolismo , Metaloproteinase 3 da Matriz/metabolismo , Prostaglandinas E/metabolismo , Coelhos , Membrana Sinovial/patologia , Microtomografia por Raio-XRESUMO
A new triterpenoid, 3,4-seco-lupane-20(29)-ene-3,28-dioic acid, together with three known lignans, (-)-schisandrin B, (-)-sesamin and (-)-syringaresinol, was isolated from the pulp of Acanthopanax senticosus (Rupr. et Maxim) Harms. Their structures were elucidated by means of physicochemical properties and spectroscopic methods (1D, 2D-NMR and MS).
Assuntos
Eleutherococcus/química , Triterpenos/isolamento & purificação , Ciclo-Octanos/isolamento & purificação , Dioxóis/isolamento & purificação , Furanos/isolamento & purificação , Lignanas/isolamento & purificação , Estrutura Molecular , Plantas Medicinais/química , Compostos Policíclicos/isolamento & purificação , Triterpenos/químicaRESUMO
OBJECTIVE: To identify the metastasis-related miRNAs in hepatocellular carcinoma (HCC) cell lines. METHODS: A qRT-PCR method was established and optimized. RESULTS: All candidate metastasis associated miRNAs except miR-124a were expressed in high metastasis cell line MHCC97H and low metastasis cell line MHCC97L, while some miRNAs were differentially expressed between liver cancer cell line (HepG2) and hepatic cell line (L02) (P less than 0.05), these miRNAs include: miR-148b (1.96+/-0.51 vs 3.76+/-0.28), miR-9 (-4.38+/-0.86 vs -1.10+/-0.53), miR-30c (8.41+/-0.40 vs 6.82+/-0.29), miR-338 (3.14+/-0.29 vs -2.36+/-0.32), miR-34a (0.71+/-0.40 vs -2.95+/-0.26), Let-7g (-4.07+/-0.55 vs -6.98+/-0.56). miR-148b expression was about 4 times higher than miR-148a [5.46 (IQR 4.25-6.67) vs 1.29 (IQR 0.94-1.64)] in all cell line tested (Z=-5.097, P=3x10(-7)). CONCLUSION: This study may help to understand the biological significance of miRNAs in HCC metastasis.
Assuntos
Carcinoma Hepatocelular/patologia , Neoplasias Hepáticas/patologia , MicroRNAs/genética , Reação em Cadeia da Polimerase , Carcinoma Hepatocelular/genética , Carcinoma Hepatocelular/metabolismo , Linhagem Celular , Linhagem Celular Tumoral , DNA Complementar/genética , Células Epiteliais/metabolismo , Humanos , Neoplasias Hepáticas/genética , Neoplasias Hepáticas/metabolismo , MicroRNAs/metabolismo , Metástase NeoplásicaRESUMO
A new compound, named 3alpha-acetoxy-7alpha-hydroxy-5beta-cholan-24-oic acid (2), along with chenodeoxycholic acid (1), was isolated from the bile of Anser anser. Their structures were elucidated by means of physicochemical properties and spectroscopic methods (1D, 2D NMR and MS).
