Mitochondrial biogenesis in white adipose tissue mediated by JMJD1A-PGC-1 axis limits age-related metabolic disease.

Mitochondria play a vital role in non-shivering thermogenesis in both brown and subcutaneous white adipose tissues (BAT and scWAT, respectively). However, specific regulatory mechanisms driving mitochondrial function in these tissues have been unclear. Here we demonstrate that prolonged activation of ß-adrenergic signaling induces epigenetic modifications in scWAT, specifically targeting the enhancers for the mitochondria master regulator genes Pgc1a/b. This is mediated at least partially through JMJD1A, a histone demethylase that in response to ß-adrenergic signals, facilitates H3K9 demethylation of the Pgc1a/b enhancers, promoting mitochondrial biogenesis and the formation of beige adipocytes. Disruption of demethylation activity of JMJD1A in mice impairs activation of Pgc1a/b driven mitochondrial biogenesis and limits scWAT beiging, contributing to reduced energy expenditure, obesity, insulin resistance, and metabolic disorders. Notably, JMJD1A demethylase activity is not required for Pgc1a/b dependent thermogenic capacity of BAT especially during acute cold stress, emphasizing the importance of scWAT thermogenesis in overall energy metabolism.

Effects of long-term different-scale rice-duck farming on the growth and yield of paddy rice.

BACKGROUND: To maintain rice production and increase revenue, rice-duck (RD) farming is a contemporary ecological cycle technology that has been widely used in Asia. However, due to the clustering activity of duck flocks, the consequences of long-term RD farming on rice growth at different scales are still unknown. Here, we studied RD farming using several different treatments (CK: conventional rice farming; RD1: 667 m2 ; RD2: 2000 m2 ; and RD3: 3333 m2 ). RESULTS: The results demonstrated that the maximum tillers, effective spikes, dry matter accumulation, and lodging index of rice under RD farming were significantly decreased by 17.9%, 9.8%, 14.8%, and 17.8%, respectively, which ultimately caused a significant decrease in yield of 10.6%. However, RD farming significantly increased root oxidation activity and the ear-bearing tiller rate of rice by 25.5% and 11.1%, respectively, and improved yield stability. For different scales of RD farming, the lodging resistance index of RD1 was significantly lower than that of RD2 and RD3 by 10.0% and 15.2%, respectively, whereas the root oxidation activity and dry matter accumulation of RD2 were significantly higher than those of RD1 and RD3 by 11.1%, 4.7%, 8.6%, and 5.1%, respectively. For rice yield, there was no significant difference among the different scales. CONCLUSION: This long-term experiment helped elucidate the complicated effects of RD farming at different scales on the growth and yield of rice. It is also critical to consider the economic advantages of different scales of RD farming to assess the impact of this system more thoroughly. © 2023 Society of Chemical Industry.

Structural characterization and in vitro fermentation properties of polysaccharides from Polygonatum cyrtonema.

Polysaccharides, the major active ingredient and quality control indicator of Polygomatum cyrtonema are in need of elucidation for its in vitro fermentation characteristics. This study aimed to investigate the structural characteristics of the homogeneous Polygomatum cyrtonema polysaccharide (PCP-80 %) and its effects on human intestinal bacteria and short chain fatty acids (SCFAs) production during the in vitro fermentation. The results revealed that PCP-80 % was yielded in 10.44 % and the molecular weight was identified to be 4.1 kDa. PCP-80 % exhibited a smooth, porous, irregular sheet structure and provided good thermal stability. The analysis of Gas chromatograph-mass spectrometer (GC-MS) suggested that PCP-80 % contained six glycosidic bonds, with 2,1-linked-Fruf residues accounted for a largest proportion. Nuclear magnetic resonance (NMR) provided additional evidence that the partial structure of PCP-80 % probably consists of →1)-ß-D-Fruf-(2 â†’ as the main chain, accompanied by side chains dominated by →6)-ß-D-Fruf-(2→. Besides, PCP-80 % promoted the production of SCFAs and increased the relative abundance of beneficial bacteria such as Megamonas, Bifidobacterium and Phascolarctobacterium during in vitro colonic fermentation, which changed the composition of the intestinal microbiota. These findings indicated that Polygomatum cyrtonema polysaccharides were able to modulate the structure and composition of the intestinal bacteria flora and had potential probiotic properties.

Release characteristic of bound polyphenols from tea residues insoluble dietary fiber by mixed solid-state fermentation with cellulose degrading strains CZ-6 and CZ-7.

The purpose of this work was to investigate the release characteristic of bound polyphenols (BP) from tea residues insoluble dietary fiber (IDF) by mixed solid-state fermentation (SSF) with cellulose degrading strains CZ-6 and CZ-7. The results implied that cellulase, ß-glucosidase and filter paper lyase activities were strongly correlated with the BP content. The scanning electron microscop and fourier transform infrared spectroscopy manifested that the cellulose network of the IDF was decomposed and dissolve, forming more loose fibrous structure. Additionally, 28 polyphenols components were detected and their biotransformation pathways were preliminary speculated. Moreover, the BP obtained by mixed SSF produced prominent inhibitory activities against α-glucosidase and α-amylase, as well as exhibited significant scavenging effects on DPPH•, ABTS+• free radicals and ferric reducing antioxidant power. These findings could further promote the utilization of BP from agricultural by-products in a more natural and economical method, CZ-6 and CZ-7 strains provide a new approach to expound the release and conversion of BP.

Study of Selective Dry Etching Effects of 15-Cycle Si0.7Ge0.3/Si Multilayer Structure in Gate-All-Around Transistor Process.

Gate-all-around (GAA) structures are important for future logic devices and 3D-DRAM. Inner-spacer cavity etching and channel release both require selective etching of Si0.7Ge0.3. Increasing the number of channel-stacking layers is an effective way to improve device current-driving capability and storage density. Previous work investigated ICP selective etching of a three-cycle Si0.7Ge0.3/Si multilayer structure and the related etching effects. This study focuses on the dry etching of a 15-cycle Si0.7Ge0.3/Si multilayer structure and the associated etching effects, using simulation and experimentation. The simulation predicts the random effect of lateral etching depth and the asymmetric effect of silicon nanosheet damage on the edge, both of which are verified by experiments. Furthermore, the study experimentally investigates the influence and mechanism of pressure, power, and other parameters on the etching results. Research on these etching effects and mechanisms will provide important points of reference for the dry selective etching of Si0.7Ge0.3 in GAA structures.

Structural properties and adsorption capacities of Mesona chinensis Benth residues dietary fiber prepared by cellulase treatment assisted by Aspergillus niger or Trichoderma reesei.

The effects of enzyme hydrolysis treatment, Aspergillus niger fermentation treatment, Trichoderma reesei fermentation treatment, Aspergillus niger-enzyme hydrolysis treatment and Trichoderma reesei-enzyme hydrolysis treatment on structural properties and adsorption capacities of soluble dietary fiber from Mesona chinensis Benth residues were evaluated and compared. The Aspergillus niger-enzyme hydrolysis treatment sample possessed more diverse structure, lower crystallinity and thermal stability than other modified samples. Meanwhile, it also observed the highest soluble dietary fiber yield (20.76 ± 0.31 %), water-holding capacity and glucose adsorption capacity (38.03 ± 0.28 mg/g). The Aspergillus niger fermentation treatment sample generated a high oil-holding capacity, nitrite ion adsorption capacity (181.84 ± 6.67 ug/g), cholesterol adsorption capacity (16.40 ± 0.37 mg/g) and sodium cholate adsorption capacity (94.80 ± 1.41 mg/g). Additionally, different monosaccharide composition was exhibited due to diverse extraction methods. Our finding revealed that these two modification methods could effectively enhance the economic value of Mesona chinensis Benth residues.

Physicochemical Characteristics of Soluble Dietary Fiber Obtained from Grapefruit Peel Insoluble Dietary Fiber and Its Effects on Blueberry Jam.

Appropriate modification methods can increase the proportion of soluble dietary fiber (SDF). In this study, grapefruit peel insoluble dietary fiber (GP-IDF) was modified with the combined microwave and enzymatic method to obtain SDF. With regard to structural characterization, SDF from grapefruit peel IDF (GP-IDF-SDF) presented as a flat sheet with cracks, composed of a typical cellulose type I crystal, and had good stability below 200 °C. Galacturonic acid, arabinose and glucuronic acid were the main monosaccharide compositions, indicating that pectin might have been the principal component. Moreover, GP-IDF-SDF was excellent in water retention capacity (13.43 ± 1.19 g/g), oil retention capacity (22.10 ± 0.85 g/g) and glucose adsorption capacity (14.49 ± 0.068 mg/g). Thereafter, the effects of GP-IDF-SDF and commercial pectin addition on the color, rheology, texture and sensory properties of blueberry jam were compared. The results showed that the color of jam with GP-IDF-SDF was lighter. The addition of GP-IDF-SDF had less effects on the viscosity and gel strength of jam, but it enhanced the stability of jam. According to sensory data, the color, texture and spreadability of jam with GP-IDF-SDF or pectin were improved and more acceptable. Overall, GP-IDF-SDF had functional characteristics and played a positive role in jam, and it is expected to be a candidate for the development of functional food ingredients.

Radiomics features of DSC-PWI in time dimension may provide a new chance to identify ischemic stroke.

Ischemic stroke has become a severe disease endangering human life. However, few studies have analyzed the radiomics features that are of great clinical significance for the diagnosis, treatment, and prognosis of patients with ischemic stroke. Due to sufficient cerebral blood flow information in dynamic susceptibility contrast perfusion-weighted imaging (DSC-PWI) images, this study aims to find the critical features hidden in DSC-PWI images to characterize hypoperfusion areas (HA) and normal areas (NA). This study retrospectively analyzed 80 DSC-PWI data of 56 patients with ischemic stroke from 2013 to 2016. For exploring features in HA and NA,13 feature sets (F method ) were obtained from different feature selection algorithms. Furthermore, these 13 F method were validated in identifying HA and NA and distinguishing the proportion of ischemic lesions in brain tissue. In identifying HA and NA, the composite score (CS) of the 13 F method ranged from 0.624 to 0.925. F Lasso in the 13 F method achieved the best performance with mAcc of 0.958, mPre of 0.96, mAuc of 0.982, mF1 of 0.959, and mRecall of 0.96. As to classifying the proportion of the ischemic region, the best CS was 0.786, with Acc of 0.888 and Pre of 0.863. The classification ability was relatively stable when the reference threshold (RT) was <0.25. Otherwise, when RT was >0.25, the performance will gradually decrease as its increases. These results showed that radiomics features extracted from the Lasso algorithms could accurately reflect cerebral blood flow changes and classify HA and NA. Besides, In the event of ischemic stroke, the ability of radiomics features to distinguish the proportion of ischemic areas needs to be improved. Further research should be conducted on feature engineering, model optimization, and the universality of the algorithms in the future.

Improving the physicochemical and in vitro hypolipidemic properties of soluble dietary fiber in camellia seed residue by a cellulose degrading fungus YC49.

Camellia seed residue (CSR) is the main by-product after edible oil extraction from camellia seed, which is a potential low-cost source of soluble dietary fiber (SDF). However, the lower yield and functional properties limit the further application of CSR SDF in the food industry. To fully utilize this resource, in this study, the cellulose degrading fungus YC49 was screened and used to modify SDF in CSR. After fermentation with YC49, the physicochemical structure and in vitro hypolipidemic properties of CSR SDF were improved. Scanning electron microscopy, X-ray diffraction, molecular weight distribution and flow properties showed that fermentation endowed F-SDF (after fermentation) with a more porous microstructure, lower crystallinity, smaller molecular weight and higher viscosity. The results of monosaccharide composition and Fourier transform infrared spectroscopy pointed to differences in the monosaccharide percentages and functional group types between CK-SDF (control) and F-SDF. These alterations combined to achieve improvements in the hydration properties and hypolipidemic activities of CSR SDF, including water holding capacity, oil retention capacity, cholesterol adsorption capacity, pancreatic lipase inhibition capacity, and bile acid binding capacity. Overall, the results indicated that YC49 could be used as an ideal strain for the fermentation modification of SDF in CSR, and F-SDF has the potential to serve as a hypolipidemic component in functional foods.

MYPT1-PP1ß phosphatase negatively regulates both chromatin landscape and co-activator recruitment for beige adipogenesis.

Protein kinase A promotes beige adipogenesis downstream from ß-adrenergic receptor signaling by phosphorylating proteins, including histone H3 lysine 9 (H3K9) demethylase JMJD1A. To ensure homeostasis, this process needs to be reversible however, this step is not well understood. We show that myosin phosphatase target subunit 1- protein phosphatase 1ß (MYPT1-PP1ß) phosphatase activity is inhibited via PKA-dependent phosphorylation, which increases phosphorylated JMJD1A and beige adipogenesis. Mechanistically, MYPT1-PP1ß depletion results in JMJD1A-mediated H3K9 demethylation and activation of the Ucp1 enhancer/promoter regions. Interestingly, MYPT1-PP1ß also dephosphorylates myosin light chain which regulates actomyosin tension-mediated activation of YAP/TAZ which directly stimulates Ucp1 gene expression. Pre-adipocyte specific Mypt1 deficiency increases cold tolerance with higher Ucp1 levels in subcutaneous white adipose tissues compared to control mice, confirming this regulatory mechanism in vivo. Thus, we have uncovered regulatory cross-talk involved in beige adipogenesis that coordinates epigenetic regulation with direct activation of the mechano-sensitive YAP/TAZ transcriptional co-activators.

Screen of high efficiency cellulose degrading strains and effects on tea residues dietary fiber modification: Structural properties and adsorption capacities.

In our study, two high efficiency cellulose degrading strains were screened, isolated and identified as Cochliobolus kusanoi and Aspergillus puulaauensis by 18S rDNA gene sequencing. In addition, the composite microbial system was constructed to develop the synergistic effect among different strains. Under the optimum conditions, the yield of soluble dietary fiber from tea residues by mixed fermentation method (MF-SDF) dramatically increased compared to single strain fermentation. The structural analysis demonstrated that all samples possessed the representative infrared absorption peaks of polysaccharides, whereas MF-SDF revealed more loose structure, lower crystallinity and smaller molecular size. For the adsorption capacities indexes, MF-SDF also owned the highest adsorbing capacity for the water molecule, oil molecule, cholesterol molecule and nitrite ion. Overall, our data showed that mixed fermentation method could be better choices to improve the functional properties of dietary fiber, and screening of cellulose degrading strains could provide new thinkings for the study of dietary fiber modification and realize high-quality utilization of crop residues.

Surface Transformation Enables a Dendrite-Free Zinc-Metal Anode in Nonaqueous Electrolyte.

Significant challenges remain in developing rechargeable zinc batteries mainly because of reversibility problems on zinc-metal anodes. The dendritic growth and hydrogen evolution on zinc electrodes are major obstacles to overcome in developing practical and safe zinc batteries. Here, a dendrite-free and hydrogen-free Zn-metal anode with high Coulombic efficiency up to 99.6% over 300 cycles is realized in a newly designed nonaqueous electrolyte, which comprises an inexpensive zinc salt, zinc acetate, and a green low-cost solvent, dimethyl sulfoxide. Surface transformation on Cu substrate plays a critical role in facilitating the dendrite-free deposition process, which lowers the diffusion energy barrier of the Zn atoms, leading to a uniform and compact thin film for zinc plating. Furthermore, in situ electrochemical atomic force microscopy reveals the plating process via a layer-by-layer growth mechanism and the stripping process through an edge-dissolution mechanism. In addition, Zn||Mo6 S8 full cells exhibit excellent electrochemical performance in terms of cycling stability and rate capability. This work presents a new opportunity to develop nonaqueous rechargeable zinc batteries.

A new approach to automatic measure fetal head circumference in ultrasound images using convolutional neural networks.

Fetal head circumference (HC) is an important biological index in prenatal ultrasound screening. In the clinic, fetal HC is usually measured manually by sonographers in two dimensional (2D) ultrasound images. The manual method is significantly affected by the inter/intra-observer difference and the process of manual measurement is inconvenient and time-consuming for sonographers. Although several artificial intelligence (AI) approaches had been applied to fetal HC measurement, they had weak generalization ability, especially for the incomplete or blurred skull edge. In this study, a fast and accurate method for fetal HC auto-measurement was proposed. Different from the common region segmentation method, an end-to-end convolutional neural network (CNN) for fetal skull boundary segmentation in 2D ultrasound images is proposed, which is an efficient method to directly segment the boundary of fetal skull by using the proposed double-branch structure. The segmentation results can be directly used to calculate fetal HC without complex post-processing. The proposed approach achieved excellent results: Mean Dice Sore (MDS)±std: 97.98 ± 1.30, Mean Hausdorff Distance (MHD)±std: 1.20 ± 0.68 mm, Mean Absolute Difference (MAD)±std: 1.75 ± 1.60 mm, Mean Difference (MD)±std: 0.08 ± 2.37 mm. Additionally, we drew a Bland-Altman plot to demonstrate that HC measured by the proposed approach has high agreement with the real value. Comprehensive results show that the proposed approach is comparable to the state-of-the-art methods for fetal HC measurement. Meanwhile, our approach belongs to a lightweight network with less parameters, which is convenient for deployment. We hope it could provide help for precision medicine in prenatal ultrasound screening.

Physicochemical structure and functional properties of soluble dietary fibers obtained by different modification methods from Mesona chinensis Benth. residue.

Alkaline hydrogen peroxide (AHP), high-temperature cooking combined with ultrasonic (HTCU) and high-temperature cooking combined with complex enzyme hydrolysis (HTCE) were used to modify soluble dietary fiber (SDF) in Mesona chinensis Benth. residue (MCBR), then the structural and in vitro functional properties of A-SDF, HU-SDF and HE-SDF were investigated. Results showed that the three treatments significantly increased the yield of SDF. Scanning electron microscopy, FT-IR, monosaccharide composition, X-ray diffraction, molecular weight distribution and thermal stability analysis were employed to determine the structural changes. Compared with the control SDF (CK-SDF), HE-SDF and HU-SDF had looser and more porous microstructure, as well as lower crystallinity. In contrast to HE-SDF and HU-SDF, A-SDF exhibited a dense wavy microstructure, and elevated crystallinity and thermal stability. In addition, the monosaccharide composition and molecular weight of HU-SDF, HE-SDF and A-SDF were significantly altered as compared to CK-SDF. Moreover, the functional properties of HE-SDF and HU-SDF, including water holding capacity (WHC), oil holding capacity (OHC), glucose adsorption capacity (GAC), α-amylase activity inhibition ratio (α-AAIR), cholesterol adsorption capacity (CAC) and nitrite ion adsorption capacity (NIAC), were significantly higher than those of CK-SDF. However, the dense structure and high crystallinity of A-SDF resulted in a significantly lower GAC and NIAC than that of CK-SDF, with only WHC and α-AAIR being improved. Overall, this study showed that HTCU and HTCE could be used as ideal modification methods for MCBR SDF, HE-SDF and HU-SDF have potential as functional additives in food.

RDHCformer: Fusing ResDCN and Transformers for Fetal Head Circumference Automatic Measurement in 2D Ultrasound Images.

Fetal head circumference (HC) is an important biological parameter to monitor the healthy development of the fetus. Since there are some HC measurement errors that affected by the skill and experience of the sonographers, a rapid, accurate and automatic measurement for fetal HC in prenatal ultrasound is of great significance. We proposed a new one-stage network for rotating elliptic object detection based on anchor-free method, which is also an end-to-end network for fetal HC auto-measurement that no need for any post-processing. The network structure used simple transformer structure combined with convolutional neural network (CNN) for a lightweight design, meanwhile, made full use of powerful global feature extraction ability of transformer and local feature extraction ability of CNN to extract continuous and complete skull edge information. The two complement each other for promoting detection precision of fetal HC without significantly increasing the amount of computation. In order to reduce the large variation of intersection over union (IOU) in rotating elliptic object detection caused by slight angle deviation, we used soft stage-wise regression (SSR) strategy for angle regression and added KLD that is approximate to IOU loss into total loss function. The proposed method achieved good results on the HC18 dataset to prove its effectiveness. This study is expected to help less experienced sonographers, provide help for precision medicine, and relieve the shortage of sonographers for prenatal ultrasound in worldwide.

A novel lung radiomics feature for characterizing resting heart rate and COPD stage evolution based on radiomics feature combination strategy.

The resting HR is an upward trend with the development of chronic obstructive pulmonary disease (COPD) severity. Chest computed tomography (CT) has been regarded as the most effective modality for characterizing and quantifying COPD. Therefore, CT images should provide more information to analyze the lung and heart relationship. The relationship between HR variability and PFT or/and COPD has been fully revealed, but the relationship between resting HR variability and COPD radiomics features remains unclear. 231 sets of chest high-resolution CT (HRCT) images from "COPD patients" (at risk of COPD and stage I to IV) are segmented by the trained lung region segmentation model (ResU-Net). Based on the chest HRCT images and lung segmentation images, 231 sets of the original lung parenchyma images are obtained. 1316 COPD radiomics features of each subject are calculated by the original lung parenchyma images and its derived lung parenchyma images. The 13 selected COPD radiomics features related to the resting HR are generated from the Lasso model. A COPD radiomics features combination strategy is proposed to satisfy the significant change of the lung radiomics feature among the different COPD stages. Results show no significance between COPD stage Ⅰ and COPD stage Ⅱ of the 13 selected COPD radiomics features, and the lung radiomics feature Y1-Y4 (P > 0.05). The lung radiomics feature F2 with the dominant selected COPD radiomics features based on the proposed COPD radiomics features combination significantly increases with the development of COPD stages (P < 0.05). It is concluded that the lung radiomics feature F2 with the dominant selected COPD radiomics features not only can characterize the resting HR but also can characterize the COPD stage evolution.

Light-Induced Redox Looping of a Rhodium/Cex WO3 Photocatalyst for Highly Active and Robust Dry Reforming of Methane.

Dry reforming of methane (DRM) has provided an effective avenue to convert two greenhouse gases, CH4 and CO2 , into syngas. Here, we design a DRM photocatalyst Rh/Cex WO3 that invokes both photothermal and photoelectric processes, which overcomes the thermodynamic limitation of DRM under conventional conditions. In contrast to plasmonic or UV-response photocatalysts, our photocatalyst produces a superior light-to-chemical energy efficiency (LTCEE) of 4.65 % with a moderate light intensity. We propose that a light-induced metal-to-metal charge transfer plays a crucial role in the DRM reaction, which induces a redox looping between Ce to W species to lower the activation energy. Quantum mechanical studies reveal that a high oxygen mobility of Cex WO3 , accompanied with the formation of oxo-bridge species, results in a substantial elimination of deposited C species during the reaction. Our catalyst design strategy could offer a promising energy-efficient industrial process for DRM.

Spatiotemporal dynamics of SETD5-containing NCoR-HDAC3 complex determines enhancer activation for adipogenesis.

Enhancer activation is essential for cell-type specific gene expression during cellular differentiation, however, how enhancers transition from a hypoacetylated "primed" state to a hyperacetylated-active state is incompletely understood. Here, we show SET domain-containing 5 (SETD5) forms a complex with NCoR-HDAC3 co-repressor that prevents histone acetylation of enhancers for two master adipogenic regulatory genes Cebpa and Pparg early during adipogenesis. The loss of SETD5 from the complex is followed by enhancer hyperacetylation. SETD5 protein levels were transiently increased and rapidly degraded prior to enhancer activation providing a mechanism for the loss of SETD5 during the transition. We show that induction of the CDC20 co-activator of the ubiquitin ligase leads to APC/C mediated degradation of SETD5 during the transition and this operates as a molecular switch that facilitates adipogenesis.

Hybrid MgCl2/AlCl3/Mg(TFSI)2 Electrolytes in DME Enabling High-Rate Rechargeable Mg Batteries.

Rechargeable magnesium batteries (RMBs) are considered as one of the most promising next-generation secondary batteries due to their low cost, safety, dendrite-free nature, as well as high volumetric energy density. However, the lack of suitable cathode material and electrolyte is the greatest challenge facing practical RMBs. Herein, a hybrid electrolyte MgCl2/AlCl3/Mg(TFSI)2 (MACT) in dimethyl ether (DME) is developed and exhibits excellent electrochemical performance. The high ionic conductivity (6.82 mS cm-1) and unique solvation structure of [Mg2(µ-Cl)2(DME)4]2+ promote the fast Mg kinetics and favorable thermodynamics in hybrid Mg salts and DME electrolyte, accelerating mass transport and the charge transfer process. Therefore, the great rate capability can be realized both in symmetric Mg/Mg cell and in CuS/Mg full cell.

Modeling and simulation of musculoskeletal system of human lower limb based on tensegrity structure.

In this paper, a mechanical model of the skeletal muscle of human lower limb system is established by using the Hill muscle model and kinetic equation of the movement of lower extremities according to the attachment positions of skeletal muscle. State vector and neural control are delineated by the direct configuration method. Changes of gait and skeletal muscle stress during walking process are analyzed with energy consumption as objective function. Results illustrate that simulation data are in good agreement with actual walking gait data. Feasibility and correctness of the designed model and control behavior of skeletal muscle tension structure are also verified.