Exploring the Catalytic Flexibility and Reversibility of Plant Glycosyltransferase HtUGT72AS1 for Glycodiversification of Phenolic Compounds.

Plant bioactive metabolites such as flavonoids are usually present in glycosylated forms by the attachment of various sugar groups. In this study, a catalytically flexible and reversible glycosyltransferase (HtUGT72AS1) was cloned and characterized from Helleborus thibetanus. HtUGT72AS1 could directly accept six sugar donors (UDP-glucose/-arabinose/-galactose/-xylose/-N-acetylglucosamine/-rhamnose) to catalyze the 3-OH glycosylation of flavonols. It also catalyzed the 4' and 7-OH glycosylation of other types of flavonoids, which lacked the 3-OH group. Additionally, the HtUGT72AS1-catalyzed reaction was highly reversible when using 2-chloro-4-nitrophenyl glycosides as substrates, which could be used for one-pot or coupled production of bioactive glycosides. It is the first reported UGT for the synthesis of arabinosides and galactosides using a transglycosylation platform. Based on structural modeling and mutagenetic analysis, the mutation of Tyr377 to Ara enhanced the catalytic efficiency of HtUGT72AS1 toward UDP-N-acetylglucosamine, and the V146S mutant gained an improvement in the regioselectivity toward 7-OH of flavonoids.

Multifunctional Carbon Nanocomposites as Nanoneurons from Multimode and Multianalyte Sensing to Molecular Logic Computing, Steganography, and Cryptography.

Inspired by information exchange and logic functions of life based on molecular recognition and interaction networks, ongoing efforts are directed toward development of molecular or nanosystems for multiplexed chem/biosensing and advanced information processing. However, because of their preparation shortcomings, poor functionality, and limited paradigms, it is still a big challenge to develop advanced nanomaterials-based systems and comprehensively realize neuron-like functions from multimode sensing to molecular information processing and safety. Herein, using fish scales derived carbon nanoparticles (FSCN) as a reducing agent and stabilizer, a simple one-step synthesis method of multifunctional silver-carbon nanocomposites (AgNPs-FSCN) is developed. The prepared AgNPs-FSCN own wide antibacterial and multisignal response abilities in five channels (including color, Tyndall, absorption and fluorescence intensities, and absorption wavelength) for quantitative colorimetric and fluorescence sensing of H2 O2 , ascorbic acid, and dopamine. Benefiting from its multicoding stimuli-responsive ability, molecular concealment, and programmability, AgNPs-FSCN can be abstracted as nanoneurons for implementing batch and parallel molecular logic computing, steganography, and cryptography. This research will promote the preparation of advanced multifunctional nanocomposites and the development of their multipurpose applications, including the multireadout-guided multianalyte intelligent sensing and sophisticated molecular computing, communication, and security.

miR-34b/c regulates doxorubicin-induced myocardial cell injury through ITCH.

Objective: To determine the underlying mechanism of miR-34b/c in regulating doxorubicin (Dox)-induced myocardial cell injury.Methods: The viability of mouse myocardial cells HL-1 was detected by MTT assay. The apoptosis of HL-1 cells was detected by TUNEL assay. mRNA expressions of ITCH, TNF-α and IL-6 were measured by qRT-PCR. Protein levels of ITCH, NF-κB, TNF-α and IL-6 were measured by western blot. Dual luciferase assay was performed to detect the regulation of miR-34b/c on ITCH. Mouse model of cardiomyopathy was induced by intraperitoneal injection of Dox.Results: Dox reduced HL-1 cell viability and activated NF-κB pathway in HL-1 cells. miR-34b/c expressions were gradually up-regulated and ITCH expression was gradually down-regulated in Dox-treated HL-1 cells. miR-34b/c expression had negative correlation with the mRNA expression of ITCH. Besides, ITCH was a target of miR-34b/c. miR-34b/c mimic reduced cell viability, suppressed ITCH expression, increased TNF-α and IL-6 level, and promoted NF-κB expression in nucleus and cytoplasm of HL-1 cells. Whereas silencing miR-34 protected HL-1 cells through regulating ITCH. Finally, we demonstrated miR-34 antagomir-protected myocardial cells in mouse model of cardiomyopathy.Conclusion: miR-34b/c decreased HL-1 cell viability and promoted the secretion of proinflammatory cytokines in Dox-induced myocardial cells through ITCH/NF-κB pathway.

[Correlation of the Serum bFGF Level with MDS Typing, Serum IL-32 Level and Prognosis].

OBJECTIVE: To investigate the correlation of serum bFGF level with MDS typing, serum IL-32 and prognosis. METHODS: A total of 62 patients with myelodysplastic syndromes admitted in our hospital from April of 2014 to April of 2017 were enrolled in MDS group. And 50 healthy people who received healthy physical examination in our hospital were selected as the control group at the same period. According to the type of disease, the MDS group was divided into 4 subgroups: A, B, C, D. the serum levels of bFGF, and IL-32 were detected, and the prognosis of patients with different MDS types was evaluated. RESULTS: The serum levels of bFGF and IL-32 in 4 subgroups of MDS were higher than those in the control group (P<0.05). the serum level of bFGF and IL-32 was not significanty different between subgroup A and B (P>0.05), and the serum levels bFGF and IL-32 also was no significantly different between subgroup C and D (P>0.05), but the serum levels of bFGF and IL-32 in subgroup C and D were significantly higher than those in subgroup A and B (P<0.05). The total efficiency of clinical treatment was not significantly different between subgroup A and B (P>0.05),but significantly higher than that in the other 2 subgroups (P<0.05). CONCLUSION: The serum levels of bFGF and IL-32 in MDS patients were significantly higher than that in the normal controls, while the serum levels of bFGF and IL-32 in RAEB and RAEB-t subtypes were significantly higher than that in RA and RAS. The changes of serum bFGF and IL-32 levels are positively correlative.

A new species group in the genus Dichaetophora, with descriptions of six new species from the Oriental region (Diptera, Drosophilidae).

The genus Dichaetophora Duda comprises 61 described species classified into four species groups: agbo, tenuicauda, acutissima and sinensis. This genus is distributed exclusively in the Old World, and is rich in species in the tropical and subtropical areas of the Oriental, Australasian, and Afrotropical regions. In this paper, a new species group, the trilobita group, is established for six new species discovered from the Oriental region. The delimitation of these species is firstly performed in light of morphology and further with the aid of DNA sequences of the mitochondrial COI and COII (cytochrome c oxydase, subunits I and II, respectively) genes, considering also their respective geographical origins. Then, the new species (trilobita Yang & Gao, sp. n., heterochroma Yang & Gao, sp. n., flatosternata Yang & Gao, sp. n., borneoensis Yang & Gao, sp. n., javaensis Yang & Gao, sp. n., and sumatraensis Yang & Gao, sp. n.) are described, and a key, based on not only morphological but also molecular information, is provided.

[Biological Characteristics and Risk Factors for Prognosis of Myelodysplastic Syndrome].

OBJECTIVE: To investigate the biological characteristics and risk factors for prognosis of patients with myelodysplastic syndrome (MDS), to emphatically study the correlation of different chromosome karyotype and immune phenotype with the prognosis of MDS patients. METHODS: All clinical data of 60 patients diagnosed as primary MDS over a period of 5 years were collected and compared. The bone marrow short-term culture and G banding technique were used to analyze the chromosome karyotypes of 60 patients, the flow cytometry was used to detect the immune phenotypes(CD10, CD13, CD15, CD7, CD34, CD117). SPSS 19.0 software was applied to deal with all data, the WHO2008 classification and IPSS-R score were used for grouping of MDS. RESULTS: There were 12 cases of low-risk, and 7 cases of high-risk in 60 patients with MDS; 35 out of 60 patients (58.33%) showed karyotypic abnormalities. The proportion of 5, 7, 8, 20, and Y chromosome abnormality were 22.89%, 20.0%, 17.14%, 11.43% and 5.71%, respectively, complex chromosome karyotype accounted for 14.29%. The percentage of normal karyotype transformed into leukemia was 8.0%, the percentage of other abnormal karyotype transformed into leukemia respectively was 80% for complex karyotype, 66.67% for 8 chromosome abnormality, 71.43% for 7 chromosome abnormality, 75.0% for 5 chromosome abnormality. The expression rate of immature myeloid cell surface antigen markers(CD13, CD33, CD117) in high-risk group of patients with MDS significantly higher than that in low-risk group(P<0.05). And the expression rate of mature myeloid cell surface antigen markers (CD15) in high-risk group was significantly lower than that in low-risk group(P<0.05). The expression rate of lymphoid cell surface antigen markers (CD7, CD10) in high-risk group was lower than that in low-risk group(P<0.05). CONCLUSION: Chromosome karyotypes and immune phenotypes have great influence on the prognosis of patients with MDS.

Construction and functional analysis of an anti-human cervical carcinoma/anti-human CD3 single-chain bispecific antibody.

The aim of the present study was to construct a single-chain bispecific antibody (scBsAb) against cervical carcinoma and to investigate its biological activities. The scBsAb was constructed using a genetic cloning technique and antigen binding activities were detected by ELISA. The iodogen method was used to analyze the pharmacokinetics. The Rosette formation test was used to detect the binding ability between peripheral blood lymphocytes (PBLs) and Cs1213 cervical cancer cells. In addition, the MTT method was performed to detect the killing effect of PBLs. The molecular weight of the scBsAb was ~60 kDa. The antigen binding activities of scBsAbs were compared with the anti­human cervical carcinoma antibody single­chain Fv fragment (CSAs­1 scFv) and anti­cluster of differentiation (CD)3 scFv (P>0.05). In addition, a pharmacokinetics assay demonstrated that compared with the two corresponding scFvs, scBsAbs exhibited a significantly prolonged retention time in the body (P<0.01). In addition, the number of rosettes formed by PBLs and Cs1213 cells in the scBsAb group was markedly greater than that in the scFv groups or the RPMI­1640 group (P<0.05 and P<0.01, respectively). The killing activity of PBLs against scBsAb­mediated Cs1213 cells was significantly greater than that mediated by the other antibodies (P<0.05). When the concentration of scBsAb was 40 µg/ml, the killing rate was 64.5%. Thus, anti­human cervical carcinoma/anti­CD3 scBsAbs may possess two types of antigen binding activity, prolong the duration in vivo and improve the killing activity of PBLs against cancer cells.

Granary Temperature Measurement Network Based on Chirped FBG.

In conventional optical fiber grating temperature measuring system, it can be loaded into a small number of fibers grating probe. At the same time, the intensity of back waves is relatively weak, and its multiplexing capability is poor. In order to solve these problems, temperature measurement system was designed based on chirped Fiber Bragg Grating. Its purpose is to obtain large-scale, multi-point temperature measurement data. The bandwidth of back waves was improved by chirp modulation techniques, so that available processing power of signal was increased, and the number of the chirped FBG probe in one fiber was greatly increased. Grating period expression was derived in chirp modulation, and modulation method and the wavelength range was provided. In the experiment, LPT-102 broadband light source and the FP optical fiber demodulator were used, and the modulation bandwidth of the system was from 1 535.0 to 1 555.0 nm. It used the WR-201 type temperature sensor as calibrated detector. Experimental results show that when the temperature changed by 1 ℃ from 20~60 ℃, the test temperature error would be closed with traditional Fiber Bragg Grating probe and chirped Fiber Bragg Grating probe, and they both meet the design requirements. In contrast, the wavelength shift data of chirped FBG was more monotone linear than the characteristic FBG, so its data was more stable. Meanwhile, in one fiber, the number of probes in the chirped FBG system was greatly more than the Uniform FBG system. In the original FBG system, without increasing the number of optical fiber or reduced the temperature measurement accuracy, design requirements for increase with the number of probe points in the system was achieved.

[Effect of Acupuncture on c-fos Expression in the Lung Tissue of Bronchial Asthmatic Rats].

Objective To observe the effect of acupuncture on c-fos expression in the lung tissue of asthmatic rats. Methods Totally 70 SPF grade male SD rats were randomly divided into 7 groups, i.e., the blank group (A) , the asthma model group (B) , the blank control group (C) , the asthma-model acupuncture control group (D) , the asthma model acupuncture group ( E) , the asthma model sham-acu- puncture group (F) , the blank acupuncture group (G) , 10 rats in each group. Corresponding interventions were performed to each group. The protein expression of c-fos in lung tissue of rats was detected u- sing immunohistochemistry and Western blot respectively. Results Immunohistochemistry showed negative expression of c-fos protein in Group A, C, G, and E, and weakly positive in Group B, D, and F. Results of Western blot showed the protein expression of c-fos was higher in Group B than in Group A and E (P <0. 01). The protein expression of c-fos was lower in Group E than in Group D and F (P <0. 05, P < 0. 01). Conclusion Acupuncture could reduce the protein expression of c-fos in lung tissue, thus attenu- ating inflammation reaction.

[Comparison of thromboelastography and routine coagulation tests for evaluation of blood coagulation function in patients].

OBJECTIVE: To investigate the correlation and consistency between thromboelastography(TEG) and routine coagulation tests, and to evaluate the value of the two methods in determining the blood coagulation of patients. METHODS: The TEG, routine coagulation tests and platelet counts of 182 patients from the Intensive Care Unit(ICU) and Department of Gastroenterology in our hospital from January to September 2014 were performed and analyzed retrospectively for their correlation, Kappa identity test analysis and chi-square test, and the diagnostic sensitivity and specificity of both methods in the patients with bleeding were evaluated. RESULTS: The TEG R time and PT, R time and APTT showed a linear dependence (P<0.01). The relationship between the TEG K value, α-Angle, MA and Fibrinogen showed a linear dependence (P<0.001). And the relationship between the TEG K value, α-Angle, MA and the platelet count were in a linear dependent way (P<0.001). The Kappa values of the TEG R time with PT and APTT were 0.038 (P>0.05) and 0.061 (P>0.05), respectively. The chi-square test values of the TEG R time with PT and APTT were 35.309 (P<0.001) and 15.848 (P<0.001), respectively. The Fibrinogen and the TEG K value, α-Angle, MA value had statistical significance (P<0.001), with a Kappa value of 0.323, 0.288 and 0.427, respectively. The chi-square test values between Fibrinogen and the TEG K value, α-Angle, MA value were not statistically significant, with X2=1.091 (P=0.296), X2=1.361 (P=0.243), X2=0.108 (P=0.742). The Kappa values of the platelet count and the TEG K value, α-Angle, MA value were 0.379, 0.208 and 0.352, respectively, which were also statistically significant difference (P<0.001). The chi-square test values between the platelet count and the TEG K value, α-Angle, MA value showed a statistically significant difference (P<0.001), with X2=37.5, X2=37.23, X2=26.630. The diagnostic sensitivity of the two methods for the patients with bleeding was less than 50%. CONCLUSION: There was a significant correlation between some TEG parameters and routine coagulation tests, but the consistency is weak. Moreover, the diagnostic sensitivity of two methods in the patients with bleeding is low. It was concluded that the TEG cannot replace the conventional coagulation tests, and the preferable method remains uncertain which could reflect the risk of bleeding.

Association of IL33/ST2 signal pathway gene polymorphisms with myocardial infarction in a Chinese Han population.

This study investigated the relationship between IL-33/ST2 signal pathway gene polymorphisms and myocardial infarction (MI) in Han Chinese. A case-control association analysis was performed on a total of 490 MI patients (MI group) and 929 normal subjects (NC group). Sequenom Mass Array and Taqman genotyping technique were used to analyze the tag single nucleotide polymorphisms (SNPs) in the genes encoding IL-33, ST2, and IL-1RaP (rs11792633, rs1041973 and rs4624606). The results showed that the frequencies of rs4624606 genotypes AA, TT, AT were 0.031, 0.647, 0.322 in MI group and 0.026, 0.712, 0.263 in NC group, and the allele frequencies of A and T were 0.192, 0.808 in MI group and 0.157, 0.843 in NC group. There were significant differences in rs4624606 genotypes and allele frequencies between MI group and NC group (P<0.05). For rs11792633, the allele frequencies of C and T were 0.45, 0.55 in MI group and 0.454, 0.546 in NC group with no significant differences found between the two groups. Compared with genotype CC+TC, rs11792633 genotype TT had an increased risk of hypertension (P<0.05). However, there were no significant differences in the frequencies of rs11792633 genotypes between the two groups. No significant differences were noted in the frequencies of rs1041973 genotype and allele between the two groups. Logistic regression analysis showed that rs4624606 genotypes AT and AA+AT were both significantly associated with MI (AT: OR=1.325, P=0.029, 95% CI=1.03-1.705; AA+AT: OR=1.316, P=0.028, 95% CI=1.03-1.681) after factors such as age, gender, smoking, drinking, body mass index (BMI), triglyceride (TG) and cholesterol were adjusted. Those carrying rs4624606 genotype AT or AA+AT had an increased risk of MI. No associations were found between the polymorphisms of the other two loci with MI. It was concluded that, in the IL33/ST2 signal pathway, the A allele of rs4624606 polymorphism of IL-1RaP gene is a potential independent risk factor for MI, and the genotypes AA+AT and AT are associated with the incidence of MI.

Expansion of myeloid-derived suppressor cells in patients with acute coronary syndrome.

AIM: The aim of this study was to explore whether the circulating frequency and function of myeloid-derived suppressor cells (MDSCs) are altered in patients with acute coronary syndrome (ACS). METHODS: The frequency of MDSCs in peripheral blood was determined by flow cytometry, and mRNA expression in purified MDSCs was analyzed by real-time reverse transcription polymerase chain reaction (RT-PCR). The suppressive function of MDSCs isolated from different groups was also determined. The plasma levels of certain cytokines were determined using Bio-Plex Pro™ Human Cytokine Assays. RESULTS: The frequency of circulating CD14(+)HLA-DR(-/low) MDSCs; arginase-1 (Arg-1) expression; and plasma levels of interleukin (IL)-1ß, IL-6, tumor necrosis factor (TNF)-α, and IL-33 were markedly increased in ACS patients compared to stable angina (SA) or control patients. Furthermore, MDSCs from ACS patients were more potent suppressors of T-cell proliferation and IFN-γ production than those from the SA or control groups at ratios of 1:4 and 1:2; this effect was partially mediated by Arg-1. In addition, the frequency of MDSCs was positively correlated with plasma levels of IL-6, IL-33, and TNF-α. CONCLUSIONS: We observed an increased frequency and suppressive function of MDSCs in ACS patients, a result that may provide insights into the mechanisms involved in ACS.

[Research on spectrum correction algorithm of temperature measurement system based on FBG].

In order to solve the problem that temperature sensor laying is complex and maintenance cost is high in the large-scale, multi-point real-time temperature monitoring process, the temperature monitoring system based on Fiber Bragg Grating was designed and developed. Using wavelength selectivity by optical fiber diffraction grating, a function of temperature and wavelength was established. Temperature of the measured position was inversed by calculating the wavelength variation through the optical fiber Bragg grating. Due to environmental, materials and other factors, the spectral distribution and temperature changes do not satisfy linear relationship. Therefore, designed the spectrum correction algorithm was designed, and function curve fitting of wavelength and temperature was completed with a degree of fitting greater than 99.7%. Experiments used FB136L-IAC-proof oven, LPT-200 diode, and 1 550 nm optical fiber to detect 20 to 280 degrees C temperature range of multi-point in real-time. The results show that when the temperature is changed by 1 degrees C, the corresponding center wavelength shifts about 0.04 nm to longer wavelengths. Compared with the test data from standard device, the error is less than +/- 0.3 degrees C. Meanwhile, the spectral correction algorithm was applied to the system to further improve the uniformity and accuracy of the temperature detection. Because the system uses fiber-optic sensor network, it has a strong anti-electromagnetic interference capability. The diffraction grating can achieve precise measurements, so it has big dynamic range and high accuracy. The innovation of the system is to ensure high-precision measurements, while still satisfy large-scale, multi-point, high anti-jamming capability of rapid laying, and has a strong practical value.

Analysis of clinical characteristics and differential diagnosis of the lung biopsy specimens in 99 adenocarcinoma cases and 111 squamous cell carcinoma cases: utility of an immunohistochemical panel containing CK5/6, CK34ßE12, p63, CK7 and TTF-1.

This study aimed at evaluating the utility of a panel of antibodies, consisting of cytokeratins (CK) 5/6, CK34ßE12, p63, thyroid transcription factor-1 (TTF-1), and CK7 for distinguishing between squamous cell carcinoma (SCC) and adenocarcinomas (Ad), as well as their expression with clinicopathological parameters and prognosis in SCC and Ad. 111 SCC of small biopsy specimens and 99 cases of Ad were stained by immunohistochemistry, among which 76 SCC and 64 Ad had complete follow-up data. Most of SCC displayed CK5/6 (91/99, 91.92%), CK34ßE12 (83/99, 83.84%), p63 (96/99, 96.97%), and most of Ad showed expression of CK7 (89/111, 80.18%) and TTF-1 (105/111, 94.59%). The combination of CK5/6/CK34ßE12/p63 seems to be useful for differentiating SCC from Ad with 100% sensitivity and 97.30% specificity, and TTF-1 was a useful biomarker for Ad with 94.59% sensitivity and 100% specificity. There were differences between CK5/6, p63, and TTF-1 expression with tumor differentiation (p<0.05) in SCC or Ad. Univariate analysis indicated that patients with high TTF-1 expression predicted better prognosis in Ad patients. Multivariate analysis showed that TTF-1 expression (HR=0.340, 95% CI 0.143-0.811, p=0.015) was a good independent predictor of Ad patient survival.

Regulatory T cells protect fine particulate matter-induced inflammatory responses in human umbilical vein endothelial cells.

OBJECTIVE: To investigate the role of CD4(+)CD25(+) T cells (Tregs) in protecting fine particulate matter (PM-) induced inflammatory responses, and its potential mechanisms. METHODS: Human umbilical vein endothelial cells (HUVECs) were treated with graded concentrations (2, 5, 10, 20, and 40 µg/cm(2)) of suspension of fine particles for 24h. For coculture experiment, HUVECs were incubated alone, with CD4(+)CD25(-) T cells (Teff), or with Tregs in the presence of anti-CD3 monoclonal antibodies for 48 hours, and then were stimulated with or without suspension of fine particles for 24 hours. The expression of adhesion molecules and inflammatory cytokines was examined. RESULTS: Adhesion molecules, including vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1), and inflammatory cytokines, such as interleukin (IL-) 6 and IL-8, were increased in a concentration-dependent manner. Moreover, the adhesion of human acute monocytic leukemia cells (THP-1) to endothelial cells was increased and NF- κ B activity was upregulated in HUVECs after treatment with fine particles. However, after Tregs treatment, fine particles-induced inflammatory responses and NF- κ B activation were significantly alleviated. Transwell experiments showed that Treg-mediated suppression of HUVECs inflammatory responses impaired by fine particles required cell contact and soluble factors. CONCLUSIONS: Tregs could attenuate fine particles-induced inflammatory responses and NF- κ B activation in HUVECs.

Hollow melon-seed-shaped lithium iron phosphate micro- and sub-micrometer plates for lithium-ion batteries.

Melon-seed-shaped LiFePO4 hollow micro- and sub-micrometer plates have been synthesized via a polyol-assisted hydrothermal method. The as-prepared LiFePO4 hollow materials were new with regard to their single-crystalline shells with large ac surfaces. Based on the detailed analysis of time-dependent studies, a possible growth mechanism was proposed involving nucleation, anisotropic growth, selective etching, and reversed recrystallization. The effects of polyol concentration, reaction temperature, and feeding sequence of precursors on the growth of LiFePO4 materials were investigated. The electrochemical properties of as-prepared LiFePO4 hollow materials were examined as cathode materials.

[Imported B3 genotype measles viruses were isolated from measles cases in the Chinese mainland].

We isolated and identified the genotypes and molecular characteristics of the imported B3 measles virus (MeV) in the Chinese mainland. The Vero/SLAM cell line was used to isolate the viruses. Reverse transcription-polymerase chain reaction was undertaken to amplify the 450 nucleotide acids of the 3-terminal of the nucleoprotein gene. A phylogenetic tree was constructed and similarities in homology assessed. Results suggested that the Shanghai isolates MVi/Shanghai. CHN/38. 13/02 [B3] and MVi/Shanghai. CHN/40. 13/02[B3] were clustered within the same genotype group as the World Health Organization (WHO) B3 genotype reference strain. The number of differences in nucleotide acids between the two Shanghai isolates was one. The homology of nucleotide acids between the Shanghai isolates and the WHO B3 genotype reference strain (MVi/Ibadan. NGA/0.97/1/B3) was 98%. Comparative results from the Measles Nucleotide Surveillance system suggested that the sequences of Shanghai isolates and the 2013 vi- ruses from Australia, Japan, Korea, Hong Kong China, Philippines and Iran were identical. This is the first time that the B3 genotype of MeV in the Chinese mainland has been isolated since 1993. These data can be used to create a "baseline" of genetic information for measles viruses in China, and help to trace the transmission of measles viruses in China and the rest of the world.

[Case-control study on two different approaches for the treatment of thoracolumbar fracture without neurological symptoms].

OBJECTIVE: To investigate the clinical effects of two surgical approaches for the treatment of thoracolumbar fracture without neurological symptoms. METHODS: From January 2008 to December 2009, 40 cases with thoracolumbar fractures without neurological symptoms treated by surgery were respectively analyzed. Among the patients, there were 13 males and 27 females, with an average age of 46 years (ranged, 26 to 61 years). Twenty patients in group A treated through posterior median approach; twenty patients in group B were treated through paraspinal muscle approach. All the patients were received the same posterior spinal internal fixation (Sofamor Inc (Basis)). Operating time, blood loss, postoperative drainage, postoperative bed time, VAS score 24 and 72 hours after operation, postoperative Cobb angle correction rate, correction rate of vertebral collapse were analyzed. RESULTS: There were no significant difference in postoperative Cobb angle correction rate and vertebral collapse rate (P < 0.05); while the index such as operating time, blood loss, postoperative drainage, postoperative bed time and VAS score 24 h and 72 h after operation in group B is better than group A. CONCLUSION: Treatment of thoracolumbar fracture through posterior median approach has an advantage of minimal invasive, less bleeding and rapidly recovery, but the patients with neural symptoms and intraspinal occupying more than 1/3 is not suggested.

Syntheses, structures, photoluminescence, and theoretical studies of d(10) metal complexes of 2,2'-dihydroxy-[1,1']binaphthalenyl-3,3'-dicarboxylate.

The free solvated ligand, H(2)bna.CH(3)OH.H(2)O (1), and its dimeric complex, [Cd(2)(bna)(2)(H(2)O)(6)] (2) (bna = 2,2'-dihydroxy-[1,1']-binaphthalene-3,3'-dicarboxylate), were obtained by evaporation of the solutions, while two new d(10) metal-hydroxy cluster-based coordination polymers, namely [Cd(8)(OH)(4)(H(2)O)(10)(bna)(6)].17H(2)O (3) and [Hpy](2)[Zn(4)(OH)(2)(H(2)O)(2)(bna)(4)].2H(2)O.2CH(3)CN (4), were obtained by a hydrothermal route. All the compounds have been characterized by X-ray crystallography and photoluminescence measurements. Compound 1 consists of a three-dimensional, hydrogen-bonded supramolecular array, 2 exhibits a dimeric molecule featuring a square motif organized by two Cd(II) atoms and two bna ligands each at the corner, and 3 contains unprecedented [Cd(8)(micro(3)-OH)(2)(micro-OH)(2)(micro-H(2)O)(2)](12+) octanuclear metallacrown cores which are interlinked through bna to afford a two-dimensional structure, while 4 features layers with butterfly-shaped [Zn(4)(micro(3)-OH)(2)](6+) clusters. All the complexes display photoluminescent properties in the blue/green range. The manifestation of photoluminescence, as probed by molecular orbital calculations performed on the complexes and also on hypothetical multinuclear complexes, is attributed to a ligand-to-metal charge-transfer mechanism. In addition to presenting a new approach for the study of the photoluminescent properties of metal-cluster-based coordination polymers by using simple model compounds, the study also reveals the dominant role of the structure of the ligand over that of the d(10) metal-hydroxy (or oxy) cluster and the presence of the cluster significantly increasing the emission lifetime.