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Background: Recent studies suggest an inverse relationship between baseline levels of hepatitis B virus (HBV) DNA and on-treatment risk of hepatocellular carcinoma (HCC) in patients with chronic hepatitis B (CHB). However, data are limited to Asian cohorts, and it is unclear if similar associations hold true for non-Asians with CHB. We aimed to evaluate association of baseline HBV DNA with long-term risks of cirrhosis and HCC among a predominantly non-Asian cohort of CHB patients in the USA. Methods: Using longitudinal data from the national Veterans Affairs database, we evaluated the risk of cirrhosis or HCC among adults with non-cirrhotic CHB who are on continuous antiviral therapy, stratified by moderate levels of baseline HBV DNA (4.00 - 6.99 log10 IU/mL) vs. high levels of baseline HBV DNA (7.00 log10 IU/mL or higher). Propensity score weighting was applied, and competing risks cumulative incidence functions and Cox proportional hazards models were utilized. Results: Among 1,129 non-cirrhotic CHB patients (41% non-Hispanic White, 36% African American, mean age 57.0 years, 62.2% hepatitis B e antigen (HBeAg) positive), 585 had moderate levels of baseline HBV DNA and 544 had high HBV DNA. After propensity score weighting, no significant difference in risk of cirrhosis was observed between moderate vs. high baseline HBV DNA (4.55 vs. 5.22 per 100 person-years, hazard ratio (HR): 0.87, 95% confidence interval (CI): 0.69 - 1.09, P = 0.22), but risk of HCC was significantly higher in patients with moderate vs. high baseline HBV DNA (0.84 vs. 0.69 per 100 person-years, HR: 1.33, 95% CI: 1.09 - 1.62, P < 0.01). Conclusions: Among a national cohort of predominantly non-Asian US veterans with non-cirrhotic CHB on antiviral therapy, moderate levels of baseline HBV DNA was associated with higher risk of HCC than high HBV DNA.
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Maize is one of the world's most important staple crops, yet its production is increasingly threatened by the rising frequency of high-temperature stress (HTS). To investigate the genetic basis of anther thermotolerance under field conditions, we performed linkage and association analysis to identify HTS response quantitative trait loci (QTL) using three recombinant inbred line (RIL) populations and an association panel containing 375 diverse maize inbred lines. These analyses resulted in the identification of 16 co-located large QTL intervals. Among the 37 candidate genes identified in these QTL intervals, five have rice or Arabidopsis homologs known to influence pollen and filament development. Notably, one of the candidate genes, ZmDUP707, has been subject to selection pressure during breeding. Its expression is suppressed by HTS, leading to pollen abortion and barren seeds. We also identified several additional candidate genes potentially underly QTL previously reported by other researchers. Taken together, our results provide a pool of valuable candidate genes that could be employed by future breeding programs aiming at enhancing maize HTS tolerance.
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Locos de Características Quantitativas , Termotolerância , Zea mays , Zea mays/genética , Zea mays/fisiologia , Locos de Características Quantitativas/genética , Termotolerância/genética , Ligação Genética , Mapeamento Cromossômico , Genes de Plantas/genética , Flores/genética , Flores/fisiologia , Pólen/genética , Pólen/fisiologiaRESUMO
Preharvest sprouting (PHS) is an undesirable trait that decreases yield and quality in rice production. Understanding the genes and regulatory mechanisms underlying PHS is of great significance for breeding PHS-resistant rice. In this study, we identified a mutant, preharvest sprouting 39 (phs39), that exhibited an obvious PHS phenotype in the field. MutMap+ analysis and transgenic experiments demonstrated that OsAAH, which encodes allantoate amidohydrolase, is the causal gene of phs39 and is essential for PHS resistance. OsAAH was highly expressed in roots and leaves at the heading stage and gradually increased and then weakly declined in the seed developmental stage. OsAAH protein was localized to the endoplasmic reticulum, with a function of hydrolyzing allantoate in vitro. Disruption of OsAAH increased the levels of ureides (allantoate and allantoin) and activated the tricarboxylic acid (TCA) cycle, and thus increased energy levels in developing seeds. Additionally, the disruption of OsAAH significantly increased asparagine, arginine, and lysine levels, decreased tryptophan levels, and decreased levels of indole-3-acetic acid (IAA) and abscisic acid (ABA). Our findings revealed that the OsAAH of ureide catabolism is involved in the regulation of rice PHS via energy and hormone metabolisms, which will help to facilitate the breeding of rice PHS-resistant varieties.
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BACKGROUND & AIMS: Conflicting data exist on the impact of alcohol use on risk of liver disease progression in patients with steatotic liver disease. We aimed to evaluate the effect of longitudinal alcohol use on risk of cirrhosis among veterans with steatotic liver disease. METHODS: US veterans with steatotic liver disease were identified from January 2010 through December 2022. Alcohol use was assessed using documented Alcohol Use Disorders Identification Test-Concise (AUDIT-C) scores and categorized as no alcohol (AUDIT-C = 0), low-risk alcohol use (AUDIT-C 1-2 for women and 1-3 for men), and high-risk alcohol (AUDIT-C ≥ 3 for women and ≥ 4 for men). Incidence of cirrhosis was evaluated with competing risks Nelson-Aalen methods. Adjusted multivariable regression models evaluated risks of cirrhosis associated with baseline alcohol use and changes in alcohol use during follow-up. RESULTS: There were 1,156,189 veterans with steatotic liver disease identified (54.2% no alcohol, 34.6% low-risk alcohol, and 11.2% high-risk alcohol). Veterans with steatotic liver disease and high-risk alcohol have a 43% higher incidence of cirrhosis compared with patients reporting no alcohol use. Compared with patients with baseline high-risk alcohol who reported no change in alcohol use, those who decreased their alcohol use during follow-up experienced a 39% reduction in long-term risk of cirrhosis (hazard ratio, 0.61; 95% CI, 0.45-0.83; P < .01). CONCLUSIONS: One in 9 veterans with steatotic liver disease report concurrent high-risk alcohol use, which is associated with 43% greater risk of cirrhosis compared with no alcohol use. However, reducing alcohol use lowers risk of cirrhosis, emphasizing the importance of timely alcohol use assessment and early interventions to address high-risk alcohol use in steatotic liver disease.
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Consumo de Bebidas Alcoólicas , Cirrose Hepática , Humanos , Feminino , Masculino , Pessoa de Meia-Idade , Estados Unidos/epidemiologia , Consumo de Bebidas Alcoólicas/efeitos adversos , Consumo de Bebidas Alcoólicas/epidemiologia , Incidência , Fatores de Risco , Cirrose Hepática/epidemiologia , Cirrose Hepática/diagnóstico , Idoso , Progressão da Doença , Veteranos/estatística & dados numéricos , Medição de Risco , Fígado Gorduroso/epidemiologia , Fígado Gorduroso/diagnóstico , Estudos Longitudinais , Fatores de Tempo , Adulto , Estudos RetrospectivosRESUMO
INTRODUCTION: Hepatic steatosis is highly prevalent in people living with HIV. It remains unclear whether HIV in patients with metabolic dysfunction-associated steatotic liver disease (MASLD) is associated with greater risks of liver disease progression and cardiovascular disease (CVD). We aim to evaluate the impact of HIV infection on risks of liver and CVD outcomes among US Veterans with MASLD. METHODS: Using national Veterans Administration data from 2010 to 2022, we created a propensity score-matched cohort of MASLD patients with vs without HIV. Primary outcomes were incidence of cirrhosis and hepatocellular carcinoma (HCC) among patients with vs without HIV and patients with MASLD-HIV on antiretroviral therapy (ART) vs not on ART. Secondary outcomes included incidence of major adverse cardiovascular events and overall survival. RESULTS: The propensity-matched cohort included 920 MASLD patients with HIV and 920 MASLD patients without HIV and was similar in demographics and comorbidities. Compared with MASLD patients without HIV, incidences of cirrhosis and HCC were similar among MASLD with HIV. Compared with MASLD patients without HIV, incidence of major adverse cardiovascular event was higher among MASLD patients with HIV (5.18 vs 4.48 per 100 person-years, P = 0.03). Overall 5-year survival was significantly lower among MASLD patients with HIV and even lower among those not on ART. DISCUSSION: Among US Veterans with MASLD, concurrent HIV infection, and particularly not being on ART, is associated with greater risks of CVD and decreased overall survival. No differences in risks of cirrhosis or HCC were observed.
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BACKGROUND: Veterans may be especially susceptible to increased alcohol consumption following the COVID-19 pandemic. We aim to evaluate trends in alcohol use among US Veterans prior to, during, and following the onset of the COVID-19 pandemic. METHODS: All US Veterans utilizing Veterans Affairs health care facilities in the United States from March 1, 2018 to February 28, 2023 with ≥1 AUDIT-C score were categorized into 1) No alcohol use (AUDIT-C = 0), 2) Low-risk alcohol use (AUDIT-C 1-2 for women, 1-3 for men), and 3) High-risk alcohol use (AUDIT-C ≥ 3 for women, ≥ 4 for men). Trends in the proportion of Veterans reporting high-risk alcohol use, stratified by sex, age, race/ethnicity, and urbanicity were evaluated. RESULTS: Among a cohort of 2.15 to 2.60 million Veterans, 15.5% reported high-risk alcohol use during March 2018-February 2019, which decreased to 14.6% during the first year of the pandemic, increased to 15.2% in the second year, and then decreased to 14.9% from March 2022-February 2023. Among non-Hispanic whites, African Americans, Asians, and Hispanics, the proportion of women reporting high-risk alcohol use surpassed that of men during the onset of the pandemic and beyond. The greatest proportion of high-risk alcohol use was observed among young Veterans ages 18-39 years (17%-27%), which was consistent across all race/ethnic groups. CONCLUSIONS: High-risk alcohol use among US Veterans has increased since the COVID-19 pandemic onset, and in the third year following pandemic onset, 15% of Veterans overall and over 20% of young Veterans ages 18-39 years reported high-risk alcohol use.
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Alcoolismo , COVID-19 , Veteranos , Masculino , Humanos , Feminino , Estados Unidos/epidemiologia , Pandemias , COVID-19/epidemiologia , Alcoolismo/epidemiologia , EtnicidadeRESUMO
Male sterility is an important trait in rice for hybrid rice (Oryza sativa) breeding. However, the factors involved in dominant male sterility are largely unknown. Here, we identified a gene from Sanming dominant genic male sterile rice, named Sanming dominant male sterility (SMS), and reported that an epi-allele of this locus contributes to male sterility. Segregation analysis attributed dominant male sterility to a single locus, SMS, which we characterized using a male-sterile near isogenic line (NIL) of rice cultivar 93-11. The SMS locus was heterozygous in the male-sterile 93-11 NIL, containing an epi-allele identical to that in 93-11, and an epi-allele identical to that in rice cultivar Nipponbare, which we refer to as SMS9 and SMSN, respectively. SMS9 is silent and hyper-methylated, whereas SMSN is expressed and hypo-methylated in the 93-11 NIL. Overexpressing SMSN led to male sterility. Mutations in SMS rescued the male sterility of the 93-11 NIL. Interestingly, we observed the duplication of SMSN in Nipponbare, but did not observe the duplication of SMS9 in 93-11. Together, these findings suggest that the reduced methylation and enhanced expression of the SMSN epi-allele in the 93-11 NIL is responsible for its role in conferring dominant male sterility.
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Oryza , Infertilidade das Plantas , Alelos , Oryza/genética , Fenótipo , Melhoramento Vegetal/métodos , Infertilidade das Plantas/genéticaRESUMO
Timely antiviral therapy is critical in chronic hepatitis B (CHB) cirrhosis, to prevent further liver complications. Among a national cohort of US Veterans with CHB cirrhosis, only 52% were initiated on antiviral therapy; treatment was significantly lower among patients of non-Asian ethnicity, high-risk alcohol use, and in rural settings.
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Background & Aims: The vast majority of studies evaluating differences in on-treatment risks of hepatocellular carcinoma (HCC) in patients with chronic hepatitis B (CHB) have been conducted in Asia. Data on the course of CHB on antiviral therapy among predominantly non-Asian populations is less well described. We aimed to evaluate overall risks of cirrhosis and HCC and the influence of baseline factors on this risk among a predominantly non-Asian cohort of patients with CHB in the US. Methods: Using longitudinal data from the national Veterans Affairs database, we evaluated the incidence of cirrhosis or HCC among adults with non-cirrhotic CHB on continuous antiviral therapy. Cumulative incidence functions and adjusted Cox proportional hazards models employed competing risks methods and evaluated overall risk and predictors of developing cirrhosis or HCC while on treatment. Results: Among 2,496 patients with non-cirrhotic CHB (39.1% African American, 38.4% non-Hispanic White, 18.8% Asian, mean age 58.0 ± 13.4 years), the overall incidences of cirrhosis and HCC were 3.99 per 100 person-years (95% CI 3.66-4.35) and 0.43 per 100 person-years (95% CI 0.33-0.54), respectively. The highest incidences of cirrhosis and HCC were observed in non-Hispanic White patients (5.74 and 0.52 per 100 person-years, respectively), which were significantly higher than in Asian patients (1.93 and 0.17 per 100 person-years, respectively, p <0.0001). On multivariate regression, only baseline FIB-4 score was consistently associated with long-term risk of cirrhosis or HCC. Conclusions: Using a longitudinal cohort of predominantly non-Asian Veterans with non-cirrhotic CHB on antiviral therapy (an understudied population), we provide important epidemiological data to describe long-term risks of cirrhosis and HCC. Impact and implications: In one of the largest studies to date of a predominantly non-Asian cohort of patients with non-cirrhotic chronic hepatitis B, we provide important epidemiological data describing the long-term risks of cirrhosis and hepatocellular carcinoma among patients on antiviral therapies. Among this understudied population, the overall incidence of cirrhosis was 3.99 per 100-person-years (95% CI 3.66-4.35) and of HCC was 0.43 per 100-person-years (95% CI 0.33-0.54). These data also emphasize the importance of continued monitoring and HCC surveillance among CHB patients who are maintained on antiviral therapies.
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BACKGROUND AND AIMS: Despite the high prevalence of metabolic dysfunction-associated fatty liver disease (MAFLD), the long-term incidence of cirrhosis or hepatocellular carcinoma (HCC) among adults with MAFLD is not well described. Using a national cohort of United States Veterans, we evaluated the overall incidence and predictors of cirrhosis and HCC among adults with noncirrhotic MAFLD. METHODS: Data from the 2010 to 2022 Veterans Affairs database were used to identify adults with noncirrhotic MAFLD using established definitions. Five and 10-year incidence of cirrhosis and HCC were assessed and stratified by demographics and relevant clinical variables. Multivariate Cox proportional hazard models were utilized to determine predictors of cirrhosis and HCC. RESULTS: Among 969,253 patients with noncirrhotic MAFLD (94.5% males, 70.2% non-Hispanic white, mean age of 62.7 ± 12.2 y), the 10-year incidence of cirrhosis and HCC was 3.70% (95% CI: 3.66-3.74) and 0.69% (95% CI: 0.67-0.70), respectively. When stratified by race/ethnicity, the 10-year incidence of cirrhosis was lowest among Asians (2.63%, 95% CI: 2.37-2.88) and highest among Hispanics (4.60%, 95% CI: 4.45-4.75), a pattern also observed with HCC. Significant disparities in risk of cirrhosis or HCC were observed when stratified by sex, substance use, and comorbidities. Risks of cirrhosis and HCC were highest in patients with baseline fibrosis-4 >2.67. CONCLUSION: This large study provides important epidemiological data describing the natural history of adults with MAFLD. Disparities in risk of cirrhosis and HCC were observed by demographic and clinical characteristics, emphasizing the importance of early identification of MAFLD with modifiable high-risk features to implement earlier interventions to improve long-term outcomes.
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Microplastics (MPs) are widely detected in wastewater treatment plants (WWTPs) and natural environment, while the relationship of MPs pollution in both media is not fully understood. In this study, the occurrence of MPs in WWTPs and in surface water and soil was investigated, and their relationship was critically formulated. Results showed although wastewater treatment could effectively remove MPs (58.2%), the effluent was still the important source of MPs in the river, while sludge was not as important as the effluent of MPs in the soil. Specifically, the dominant size ranges of MPs were 0-200 µm, with main type of PE in all wastewater, sludge, river and soil. The dominant shape of MPs in wastewater and river was film. However, the shapes were different between sludge (52.1% of fibers) and soil (40.6% of fragment). Overall, WWTP input and surface runoff were the main source of MPs pollution in surface water, and the abrasion of agricultural films accounted for the MPs pollution in soil. The findings revealed the distribution and interconnection of MPs in WWTPs and environmental media, which could help to trace the sources of MPs pollution and assess the ecological risks in the environment.
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KEY MESSAGE: Two causal OsTTL and OsSAPK1 genes of the key locus qNL3.1 significantly associated with seed germination under salt stress were identified via a genome-wide association study, which could improve rice seed germination under salt stress. Rice is a salt-sensitive crop, and its seed germination determines subsequent seedling establishment and yields. In this study, 168 accessions were investigated for the genetic control of seed germination under salt stress based on the germination rate (GR), germination index (GI), time at which 50% germination was achieved (T50) and mean level (ML). Extensive natural variation in seed germination was observed among accessions under salt stress. Correlation analysis showed significantly positive correlations among GR, GI and ML and a negative correlation with T50 during seed germination under salt stress. Forty-nine loci significantly associated with seed germination under salt stress were identified, and seven of these were identified in both years. By comparison, 16 loci were colocated with the previous QTLs, and the remaining 33 loci might be novel. qNL3.1, colocated with qLTG-3, was simultaneously identified with the four indices in two years and might be a key locus for seed germination under salt stress. Analysis of candidate genes showed that two genes, the similar to transthyretin-like protein OsTTL and the serine/threonine protein kinase OsSAPK1, were the causal genes of qNL3.1. Germination tests indicated that both Osttl and Ossapk1 mutants significantly reduced seed germination under salt stress compared to the wild type. Haplotype analysis showed that Hap.1 of OsTTL and Hap.1 of OsSAPK1 genes were excellent alleles, and their combination resulted in high seed germination under salt stress. Eight accessions with elite performance of seed germination under salt stress were identified, which could improve rice seed germination under salt stress.
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Germinação , Oryza , Germinação/genética , Oryza/genética , Estudo de Associação Genômica Ampla/métodos , Sementes/genética , Estresse Salino/genéticaRESUMO
Real-time dense view synthesis based on three-dimensional (3D) reconstruction of real scenes is still a challenge for 3D light-field display. It's time-consuming to reconstruct an entire model, and then the target views are synthesized afterward based on volume rendering. To address this issue, Light-field Visual Hull (LVH) is presented with free-viewpoint texture mapping for 3D light-field display, which can directly produce synthetic images with the 3D reconstruction of real scenes in real-time based on forty free-viewpoint RGB cameras. An end-to-end subpixel calculation procedure of the synthetic image is demonstrated, which defines a rendering ray for each subpixel based on light-field image coding. In the ray propagation process, only the essential spatial point of the target model is located for the corresponding subpixel by projecting the frontmost point of the ray to all the free-viewpoints, and the color of each subpixel is identified in one pass. A dynamic free-viewpoint texture mapping method is proposed to solve the correct graphic texture considering the free-viewpoint cameras. To improve the efficiency, only the visible 3D position and texture that contributes to the synthetic image are calculated based on backward ray tracing rather than computing the entire 3D model and generating all elemental images. In addition, an incremental calibration method by dividing camera groups is proposed to satisfy the accuracy. Experimental results show the validity of our method. All the rendered views are analyzed for justifying the texture mapping method, and the PSNR is improved by an average of 11.88dB. Finally, LVH can achieve a natural and smooth viewing effect at 4K resolution and the frame rate of 25 â¼ 30fps with a large viewing angle.
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The epidemiology of latent tuberculosis and hepatitis B virus (HBV-LTBI) co-infection among U.S. populations is not well studied. We aim to evaluate LTBI testing patterns and LTBI prevalence among two large U.S. cohorts of adults with chronic HBV (CHB). Adults with CHB in the Chronic Hepatitis Cohort Study (CHeCS) and Veterans Affairs national cohort were included in the analyses. Prevalence of HBV-LTBI co-infection was defined as the number of HBV patients with LTBI divided by the number of HBV patients in a cohort. Multivariable logistic regression evaluated odds of HBV-LTBI co-infection among CHB patients who underwent TB testing. Among 6019 CHB patients in the CHeCS cohort (44% female, 47% Asian), 9.1% were tested for TB, among whom 7.7% had HBV-LTBI co-infection. Among HBV-LTBI co-infected patient, only 16.7% (n = 7) received LTBI treatment, among whom 28.6% (n = 2) developed DILI. Among 12,928 CHB patients in the VA cohort (94% male, 42% African American, 39% non-Hispanic white), 14.7% were tested for TB, among whom 14.5% had HBV-LTBI. Among HBV-LTBI co-infected patient, 18.6% (n = 51) received LTBI treatment, among whom 3.9% (n = 3) developed DILI. Presence of cirrhosis, race/ethnicity, and country of birth were observed to be associated with odds of HBV-LTBI co-infection among CHB patients who received TB testing. In summary, among two large distinct U.S. cohorts of adults with CHB, testing for LTBI was infrequent despite relatively high prevalence of HBV-LTBI co-infection. Moreover, low rates of LTBI treatment were observed among those with HBV-LTBI co-infection.
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Doença Hepática Induzida por Substâncias e Drogas , Coinfecção , Hepatite B Crônica , Hepatite B , Tuberculose Latente , Adulto , Humanos , Masculino , Feminino , Estados Unidos/epidemiologia , Vírus da Hepatite B , Tuberculose Latente/complicações , Tuberculose Latente/epidemiologia , Estudos de Coortes , Prevalência , Coinfecção/epidemiologia , Coinfecção/complicações , Hepatite B/complicações , Hepatite B/epidemiologia , Hepatite B Crônica/complicações , Doença Hepática Induzida por Substâncias e Drogas/complicaçõesRESUMO
Light irradiation is considered as most important process for the aging of microplastics (MPs); however, which factors drive the process is still unknown. This study investigated the role of typical environmental factors including ultraviolet (UV), oxygen, temperature and physical abrasion in the photoaging of polystyrene (PS) in freshwater. Results showed that UV irradiation and abrasion were dominant factors for affecting photoaging of PS based on dynamic analysis in the property of MP itself and leachate. Especially, when both factors worked together on MPs, they caused more destructive effect. Mechanical exploration revealed that photoaging of MPs was mainly controlled by reactive oxygen species (ROS, 1O2) generated from the reaction of dissolved oxygen/water molecules with polymer radicals initiated by UV energy. As an attacker on MPs, ROS formation was significantly linked with UV intensity, highlighting the important role of UV. The fragmentation was correlated to abrasion intensity, where a higher abrasion generated stronger physical force to tear MPs into fragments. The low roles of oxygen and temperature were presumably related to multiple effects of ROS formation and UV absorption. The findings firstly clarify the drivers in the photoaging of MPs, and contribute our effort to assess their fate and pollution risk in the environment.
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Microplásticos , Envelhecimento da Pele , Microplásticos/toxicidade , Plásticos , Espécies Reativas de Oxigênio , Água Doce , Poliestirenos , OxigênioRESUMO
Three-dimensional (3D) light-field displays can provide an immersive visual experience, which has attracted significant attention. However, the generating of high-quality 3D light-field content in the real world is still a challenge because it is difficult to capture dense high-resolution viewpoints of the real world with the camera array. Novel view synthesis based on CNN can generate dense high-resolution viewpoints from sparse inputs but suffer from high-computational resource consumption, low rendering speed, and limited camera baseline. Here, a two-stage virtual view synthesis method based on cutoff-NeRF and 3D voxel rendering is presented, which can fast synthesize dense novel views with smooth parallax and 3D images with a resolution of 7680 × 4320 for the 3D light-field display. In the first stage, an image-based cutoff-NeRF is proposed to implicitly represent the distribution of scene content and improve the quality of the virtual view. In the second stage, a 3D voxel-based image rendering and coding algorithm is presented, which quantify the scene content distribution learned by cutoff-NeRF to render high-resolution virtual views fast and output high-resolution 3D images. Among them, a coarse-to-fine 3D voxel rendering method is proposed to improve the accuracy of voxel representation effectively. Furthermore, a 3D voxel-based off-axis pixel encoding method is proposed to speed up 3D image generation. Finally, a sparse views dataset is built by ourselves to analyze the effectiveness of the proposed method. Experimental results demonstrate the method's effectiveness, which can fast synthesize novel views and 3D images with high resolution in real 3D scenes and physical simulation environments. PSNR of the virtual view is about 29.75 dB, SSIM is about 0.88, and the synthetic 8K 3D image time is about 14.41s. We believe that our fast high-resolution virtual viewpoint synthesis method can effectively improve the application of 3D light field display.
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Before being discharged into natural environment, almost all of microplastics (MPs) interact with wastewater constituents in wastewater treatment plants (WWTPs). This study investigated the photoaging of disposable box-derived polystyrene (PS) mediated by real wastewater by simulating the case flowing from WWTPs to natural water. Results showed that wastewater influent pretreatment significantly enhanced the photoaging of PSMPs through the sorption of wastewater constituents, e.g., 2.02 times of increase in photooxidation after 30 d of ultraviolet irradiation. Fulvic acid was identified as the leading contributor for the enhancing effect of wastewater relative to other wastewater constituents such as Cl, CO32-, NO3- and clay particles. In-depth mechanism analysis showed that the observed enhancement was critically controlled by the photosensitization effect of wastewater itself and the enhanced utilization of PSMPs for ultraviolet energy. Specifically, various sorbed wastewater constituents can not only generate higher concentrations of â¢OH and O2â - than clean MPs without constituents, but also reinforce the utilization of PSMPs for light energy due to the increased dispersion in solution by increasing hydrophilicity and surface charges. Also, the light-shielding effect was induced by wastewater, but was less important. This study bridges wastewater source and MP aging and fate and suggests the shortened lifetime of (micro)plastic samples via WWTP input to deepen our understanding of MP pollution in the environment.
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Envelhecimento da Pele , Poluentes Químicos da Água , Microplásticos , Águas Residuárias/análise , Plásticos , Poliestirenos , Água/análise , Eliminação de Resíduos Líquidos , Poluentes Químicos da Água/análise , Monitoramento AmbientalRESUMO
INTRODUCTION: The purpose of this study was to evaluate hepatitis delta virus (HDV) testing patterns among US adults with chronic hepatitis B (CHB). METHODS: HDV testing was evaluated among CHB patients using Quest Diagnostics (2016-2020) and Veterans Affairs (2010-2020) data. RESULTS: Among 157,333 CHB patients (Quest), 6.7% received HDV testing, among which 2.2% were positive. HDV testing was higher in male patients, younger individuals, and patients with advanced liver disease. Among 12,002 CHB patients (Veterans Affairs), 19.7% received HDV testing, among which 3.1% were positive. HDV testing was higher in younger individuals and Asians. DISCUSSION: Low HDV testing was observed among 2 large US cohorts of adults with CHB.
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Hepatite B Crônica , Hepatite B , Adulto , Humanos , Masculino , Estados Unidos/epidemiologia , Vírus Delta da Hepatite , Hepatite B Crônica/diagnóstico , Hepatite B Crônica/epidemiologia , Vírus da Hepatite B , Antígenos de Superfície da Hepatite BRESUMO
In orthopedics, developing functionalized biomaterials to enhance osteogenesis and bacterial resistance is crucial. Although poly(ether ether ketone) (PEEK) is regarded as an important engineering plastic for biomedical material with excellent mechanical properties and biocompatibility, its biological inertness has greatly compromised its application in biomedical engineering. Inspired by the catecholamine chemistry of mussels, we propose a universal and versatile approach for enhancing the osteogenesis and antibacterial performances of PEEK based on surface functionalization of polydopamine-modified nanohydroxyapatite and lysozyme simultaneously. The characterizations of surface morphology and elemental composition revealed that the composite coating was successfully added to the PEEK surface. Additionally, the in vitro cell experiment and biomineralization assay indicated that the composite coating-modified PEEK was biocompatible with significantly improved bioactivity to promote osteogenesis and biomineralization compared with the untreated PEEK. Furthermore, the antibacterial test demonstrated that the composite coating had a strongly destructive effect on two bacteria (Staphylococcus aureus and Escherichia coli) with antibacterial ratios of 98.7% and 96.1%, respectively. In summary, the bioinspired method for surface functionalization can enhance the osteogenesis and bacterial resistance of biomedical materials, which may represent a potential approach for designing functionalized implants in orthopedics.
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Cetonas , Osteogênese , Antibacterianos/farmacologia , Materiais Biocompatíveis/farmacologia , Éter/farmacologia , Éteres/farmacologia , Cetonas/farmacologia , Polietilenoglicóis/farmacologia , Propriedades de SuperfícieRESUMO
Glioma is a primary high malignant intracranial tumor with poorly understood molecular mechanisms. Previous studies found that both DNA methylation modification and gene alternative splicing (AS) play a key role in tumorigenesis of glioma, and there is an obvious regulatory relationship between them. However, to date, no comprehensive study has been performed to analyze the influence of DNA methylation level on gene AS in glioma on a genome-wide scale. Here, we performed this study by integrating DNA methylation, gene expression, AS, disease risk methylation at position, and clinical data from 537 low-grade glioma (LGG) and glioblastoma (GBM) individuals. We first conducted a differential analysis of AS events and DNA methylation positions between LGG and GBM subjects, respectively. Then, we evaluated the influence of differential methylation positions on differential AS events. Further, Fisher's exact test was used to verify our findings and identify potential key genes in glioma. Finally, we performed a series of analyses to investigate influence of these genes on the clinical prognosis of glioma. In total, we identified 130 glioma-related genes whose AS significantly affected by DNA methylation level. Eleven of them play an important role in glioma prognosis. In short, these results will help to better understand the pathogenesis of glioma.