Clinical and follow-up study of premature infants with neonatal respiratory distress syndrome managed by the less invasive surfactant administration / 中华实用儿科临床杂志

Objective:To explore the clinical therapeutic effect and follow-up prognosis of preterm infants with neonatal respiratory distress syndrome (NRDS) managed by less invasive surfactant administration (LISA) and traditional intubation-surfactant-extubation (INSURE) of pulmonary surfactant (PS).Methods:Data during hospitalization and follow-up period of 187 NRDS preterm infants (gestational age 24 weeks to 31 + 6 weeks, and birth weight <1 500 g) admitted to the Department of Neonatology, the Women and Children′s Hospital of Chongqing Medical University from March 2019 to February 2021 were retrospectively analyzed.NRDS preterm infants who were injected with PS by LISA were included in the LISA group (144 cases), and those who were injected with PS by INSURE were included in the INSURE group (43 cases). The propensity score matching method was used to correct the confounding factors between groups, and the covariate equilibrium samples between groups were obtained (39 cases in each group). Clinical treatment effect and prognosis of physical development, hearing and vision development, nervous system development, respiratory system diseases and other conditions of the two groups of children were compared using the t test, Chi- square test and other statistical analysis methods as appropriate. Results:(1)Compared with that of the INSURE group, the incidence of BPD [12 cases (33.3%) vs.23 cases (63.9%), χ2=6.727, P=0.009] and ROP [13 cases (36.1%) vs.26 cases (72.2%), χ2=9.455, P=0.002] in the LISA group were significantly lower.The incidence of mild BPD [8 cases (22.2%) vs.16 cases (44.4%), χ2=4.000, P=0.046] and stage Ⅰ-Ⅱ ROP [11 cases (30.6%) vs.22 cases (61.1%), χ2=6.769, P=0.009] in the LISA group was significantly lower than that of the INSURE group.There was no significant difference in the incidence of moderate and severe BPD and stageⅢ ROP and above between groups (all P>0.05). (2)There were no statistical differences in the repeated use of PS, mechanical ventilation rate within 72 h, pneumothorax/pulmonary hemorrhage, grade Ⅲ-Ⅳ periventricula-rintraventricular hemorrhage, stage Ⅱ-Ⅲ neonatal necrotizing enterocolitis, sepsis, abnormal amplitude integrated electroencephalogram, mortality in 36 weeks of corrected gestational age, total oxygen inhalation duration and hospitalization duration between the two groups (all P>0.05). (3)Follow-up within 1 year of corrected age after discharge.There were no significant differences in extrauterine body mass, body length and head circumference development, visual development, hearing development, Neonatal Behavioral Neurological Assessment score at corrected gestational age of 40 weeks, Bayley Scales of Infants Development score at corrected gestational age of 6 months and age of 1 year, pneumonia and re-hospitalization due to respiratory diseases between groups (all P>0.05). Conclusions:PS administration with LISA technology can reduce the incidence of mild BPD and stage Ⅰ-Ⅱ ROP in premature infants with NRDS who had the gestational age of 24-31 + 6 weeks and birth weight<1 500 g, without increasing the risk of other complications.The long-term prognosis of them treated with PS administration with LISA and INSURE is similar.

Advances in clinic study of thermal moxibustion in recent five years / 国际中医中药杂志

Combining with the data based on data mining,to collect the clinical experimental research ralating to thermal moxibustion from June 2010 to March 2015, and extract the representative clinical experimental research of clinical diseases. To summarize advances in clinic study of Thermal Moxibustion in recent five years.

Study of clinical application of manipulations of filiform needles to promoteby data mining technique / 中国针灸

<p><b>OBJECTIVE</b>To analyze the diseases and effects of the manipulations to promoteby filiform needles, with the help of data mining technique.</p><p><b>METHODS</b>Literature about different manipulations to promoteby filiform needles in recent 60 years was collected in China National Knowledge Infrastructure(CNKI). Then databases were established to mine the relativities among the diseases, subjects, acupoints selection law,routes and directions of meridianand effects.</p><p><b>RESULTS</b>①Total 489 articles were included. The basic manipulations with the frequency of 630 were on the top. The auxiliary and four methods ofrespectively appeared 70 times. The diseases in the internal medicine were treated with the basic and auxiliary methods of the higher frequencies, 311 and 44 times separately. While the four methods ofwere mostly used in the surgical area, 37 timestotally. ②The diseases treated from high to low by lifting, thrusting and twirling represented retention of urine, sequelae of stroke, pain in waist and lower limbs, of which the effect for retention of urine was better than those of the other two, and the lifting and thrusting method was superior to twirling. ③As for the auxiliary methods, 25 times were found about vibration needling; 18 times, twisting. The highest frequency of 6 times for the vibration needling was on the sequelae of stroke; while 4 times for the twisting, stranguria. ④As to the four methods of,was most frequently applied, and its highest frequency of 18 was onsyndrome.</p><p><b>CONCLUSIONS</b>Manipulations to promoteby filiform needles are crucial to achieving the effects of acupuncture. The basic methods are widely used in clinic and have been seen good efficacy. While the auxiliary means assist to promoteto stimulate the body,among which the four methods ofare mainly to smooth the meridians and driveof patients with surgical diseases and take meridian blocking the priority.</p>

Levels of 25-(OH)D in 0 ～ 12 years Old Children in Kunming / 昆明医科大学学报

Objective To learn the levels of serum 25-(OH)D in 0 12 years old chindren in Kunming.Methods Serum 25-(OH)D levels of children (4 498) were measured by using Roche cobas 8 000 / E602 automatic electrochemical luminescence immunoassay.Results As the increase of age,the levels of serum 25-(OH)D were gradually decreased,different age groups had significant difference (P＜0.05).The levels of 25-(OH)D between boys and girls had no significant difference (P＞0.05);The average level of sernm 25-(OH)D was 83.48 nmol/L,the level of serum 25-(OH)D was distributed in 0 ～ 1 years old group,and most concentrated in the 6 ～ 9 years old group.Conclusion The levels of 25-(OH)D in 0 ～ 12 years old chindren are in a good status,but with the increase of age,the level of 25-(OH)D is decreasing gradually,especially the levels of 25-(OH)D decreased significantly after the age of three,in shortage or lack of status,this should cause everybody's attention.

Present situation and development direction of microacupuncture therapy / 中国针灸

There have been many reports of clinical and experimental researches of microacupuncture therapy, the relevant systematic works, teaching materials and national standards in recent decades. It shows that microacupuncture system has been formed and its influence has been promoted. While the rapid development of microacupuncture therapy, there are the problems and contradictions on nomenclature, explanation of theoretical basis, optimal indications, and multiple systems and schools, etc. All the above have blocked the clinical application and development. It is considered that we need to unify the nomenclature, condense the theoretical basis, clear the optimal indications, promote the exchange and blend among different schools and sum up the outcomes by systematic review and data mining technique.

The clinical study of perioperative depression in brain tumor patients / 中国神经精神疾病杂志

Objective To investigate the clinical features and incidence of depression in patients with different lo-cations and pathological types of brain tumors. Methods Hamilton Depression Scale (HRSD) was used to assess the de-pression in 140 patients with brain tumor before and after operation. Results The preoperative mean depression rating score was 15.36 ± 6.52 and the prevalence rate of depression was 50 cases (35.7%) in 140 patients with brain tumor. The postoperative mean score of depression was 9.71 ± 5.55 and the prevalence rate of depression was 9 cases (6.4%) in 140 patients with brain tumor. The postoperative depression score and the prevalence rate was significantly decreased after op-eration (P<0.05)(χ2=36.10,P<0.05). The postoperative depression score in either benign or malignant brain tumors was significantly lower after than before operation(all P<0.05)The postoperative depression score in either the left or right or bilateral brain lesions were significantly decreased after than before operation(all P<0.05).Conclusion Pa-tients with brain tumors have different degrees of depression in perioperative. Depression may be associated with brain tu-mour pathological types and lesion sites in patients with brain tumors.

Design of a gain compensating circuit for medical ultrasonic system / 中国组织工程研究

BACKGROUND: To make the echo signals reflected by interface with different depth and same property display the same amplitude,amplifier gain much be gradually increased based on depth(time),and the situation that weak long-distance reflected wave caused by ultrasonic wave in the ultrasonic transmission attenuation process must be compensated.OBJECTIVE: To compensate the propagation attenuation of the echo signal in the medical ultrasonic system,a time gain compensating(TGC)circuit design scheme is presented,and the design basis and principles of the circuit are briefly introduced.METHODS: Aiming to the medical ultrasound system features,the variable gain amplifier parts VCA610 with high SNR,high-bandwidth were chosen to realize the ultrasonic gain compensation circuit.RESULTS and CONCLUSION: The design effectively solved the non-linear compensation of echo signals caused by echo distance in the medical ultrasound soft tissue measurement.Compared with traditional discrete components circuit,the scheme had the characteristics of simple circuit,stable and reliable TGC control signal,and flexible adjustment,etc.It can accurately compensate the attenuation of ultrasound in the human body,thus,provides a new and reliable method for the medical measurement system design.

Construction and verification of replication-deficient recombinant adenovirus to silence CTGF gene by AdEasy system / 肿瘤

Objective:To design and construct the replication-deficient recombinant adenovirus Ad-siCTGF which can silence the expression of connective tissue growth factor (CTGF) by RNA interference and verified its function. Methods:A specific sequence, which was verified to be able to silence CTGF gene with high efficiency, was cloned into pSES-HUS vector to produce the shuttle plasmid pSES-siCTGF. The plasmid after Pme Ⅰ linearization was cotransduced with pAdEasy into BJ5183 E.coli strains to construct recombinant vector Ad-siCTGF. After linearization treatment with Pac Ⅰ enzyme digestion Ad-siCTGF was transfected into HEK293 cells via liposome mediation. The recombinant adenovirus was packaged. The titer of the Ad-siCTGF was increased after three times of cross-infection. 4T1 cells were infected with the adenovirus. The silencing efficiency was tested by real-time fluorescence quantitative (RFQ)-PCR and Western blotting.Results:Pac Ⅰ enzyme digestion electrophoresis indentified that recombinant adenovirus was successfully constructed. The titer of the recombinant adenovirus Ad-siCTGF was 2.6×10~(10) pfu/mL after amplification and purification. The CTGF mRNA and protein expression in 4T1 cells were decreased by 36.27% and 31.56%, respectively, compared with the control groups.Conclusion:The recombinant adenovirus which can silence the expression of CTGF was successfully constructed. It laid a good foundation for further investigation of the action mechanism of CTGF in tumor cells.

Effect of naloxone on neural functional recovery of rats with traumatic brain injury / 中国组织工程研究

BACKGROUND: As unspecific antagonist of opiate receptor, naloxone is widely used for multiple diseases which are related with abnormal release of endogenous opium. At present, researches suggest that large dosage of naloxone is used at early period can decrease death rate of patients with acute craniocerebral injury and promote neural functional recovery.OBJECTIVE: To investigate the effect of naloxone on improving the nervous function of rats with acute craniocerebral injury and to analyze effectively.DESIGN: Randomized grouping design based on the experimental animal.SETTING: Beijing Neurosurgical Institute.MATERIALS: Totally 250 SD rats were divided randomly into 0.3, 1.0,3.0, 9.0 mg/kg naloxone group, positive control group and negative control group.METHODS: Craniocerebral injured model was established with Feenly free fall struck, and the medicine was given 30 minutes after injury. The rats of the first four experimental group were injected transpeniponeally with naloxone hydrochloride by 0.3 mg/kg, 1.0 mg/kg, 3.0 mg/kg and 9.0 mg/kg respectively once a day; meanwhile, the control groups were given 2 mg citicoline sodium for injection and 0.5 mL normal saline per rat respectively. The longest time was 14 days.MAIN OUTCOME MEASURES: MNSS neural functional score was used every day. The brain edemas of 8 rats in each group were measured with wet-dry weight methods on the second and the fourth day after head trauma.RESULTS: Among 250 rats, 172 entered the final analysis. The nervous function of rats in naloxone groups was better than the two control groups (P ＜ 0.01), and that in 1, 3 and 9 mg/kg naloxone group were better than 0.3 mg/kg group (P ＜ 0.05), but there were no significant differences a mong the three naloxone groups (P ＞ 0.05). The brain edemas of rats in naloxone groups were lighter than that in the control groups (P ＜ 0.05), and that of 1, 3 and 9 mg/kg groups were lighter than 0.3 mg/kg (P ＜ 0.05), but there were no significant differences among these three groups (P ＞ 0.05).CONCLUSION: Naloxone can decrease the brain edemas of rats with traumatic brain injury, promote the nervous function recovery, and the treatment effect changes with the dosage during some range.Therefore, the experiment illustrates that naloxone can decrease the brain edemas of experimental brain injury in SD rats and improve the nervous function, but the effect of naloxone is associated with the dosages in some range.

Effects of the Ligustrazine Injection on Retina Injury Rat Models Induced by Retina Ischemia-reperfusion / 中药新药与临床药理

Objective To investigate the therapeutic effect of the ligustrazine injection on rats models of experimental high intraocular pressure induced by retina ischemia-reperfusion.Methods Thirty two SD rats were divided into four groups randomly:the ligustrazine group,the model group,the normal group and the vitamine E group.The ischemia-reperfusion model is established by increasing intraocular pressure to 110mmHg in rat eyes.The ligustrazine group was treated with the ligustrazine injection,the vitamine E group with gastric gavage of vitamine E,and the model and normal groups with distilled water.The period of treatment was 7 days.The histopathologic changes were observed under the optical microscope and the electron microscope,meanwhile the SOD and MDA were measured.Results After ischemia for 60 min and reperfusion for 48 h,the cells in retinal ganglion cell layer and inner nuclear layer were thinned under optical microscope,perinuclear karyopyknosis and apoptotic bodies appeared in retinal ganglion cell layer and inner nuclear layer under electron microscope,SOD activity decreased and MDA level increased.After treatment,the morphology of retinal ganglion cell layer and inner nuclear layer was improved obviously,SOD activity increased and MDA level decreased.It is suggested that ligustrazine injection exerts anti-ischemic and anti-hypoxic and cytomembrane stabilizing effects to a certain extent.Conclusion The ligustrazine injection is an effective traditional Chinese medicine in treating retinal ischemia.