RESUMO
Previous studies underscored the importance of genetic factors in the pathogenesis of certain cancers, including cervical cancer. Epidemiological evidence supports an association between specific polymorphisms of Toll-like receptors (TLR) with several human pathological states, including cervical cancer. The aim of this study was to investigate the link between specific gene variants in TLR2 (-196 to -174 del), TLR3 (c.1377 C>T), TLR4 (Asp299Gly), and TLR9 (2848 G>A) and susceptibility to cervical cancer in Tunisian women. Study subjects comprised 122 women with histopathologically-confirmed cervical cancer, and 260 unrelated age- and ethnically-matched healthy females, who served as controls. TLR genotyping was done using PCR-restriction fragment length polymorphism. The C/C genotype of TLR3 (c.1377 C>T) is associated with cervical cancer susceptibility (OR: 1.71, CI: 1.08-2.70). For TLR4 (Asp299Gly), the Asp/Asp genotype and the Asp allele were associated with higher risk of developing cervical cancer (OR: 4.95, CI: 1.97-13.22) and (OR: 5.17, CI: 2.11-13.50) respectively. We demonstrated no association between the TLR2 (-196 to -174 del) and the TLR 9 (2848 G>A) polymorphisms and the susceptibility of cervical cancer among Tunisian women. However, the C/C genotype for the TLR3 (c.1377 C>T) polymorphism and the Asp/Asp genotype and the Asp allele for (Asp299Gly) TLR4 polymorphism were found to be associated with a higher risk of cervical cancer.
Assuntos
Predisposição Genética para Doença , Receptor 2 Toll-Like/genética , Receptor 3 Toll-Like/genética , Receptor 4 Toll-Like/genética , Receptor Toll-Like 9/genética , Neoplasias do Colo do Útero/genética , Adenocarcinoma/genética , Adenocarcinoma/patologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Alelos , Carcinoma de Células Escamosas/genética , Carcinoma de Células Escamosas/patologia , Estudos de Casos e Controles , Intervalos de Confiança , Feminino , Genótipo , Humanos , Pessoa de Meia-Idade , Razão de Chances , Polimorfismo Genético , Polimorfismo de Fragmento de Restrição , Análise de Regressão , Estudos Retrospectivos , Risco , Sarcoma/genética , Sarcoma/patologia , Tunísia , Neoplasias do Colo do Útero/patologiaRESUMO
Brain balance changes have been recognised in migraine, but cerebellar function between or during attacks has been assessed only in a few studies. Previous studies have indicated that migraine affects cerebellar function. In the present study we aimed to evaluate the hemicerebellar volume changes of patients with migraine without aura (MWoA). Volumetric changes of cerebellar hemispheres were evaluated in terms of asymmetry using stereological methods on magnetic resonance images (MRI) retrospectively. Nineteen patients with MWoA and 18 age- and gender-matched control subjects were included in the study. MRIs were analysed by using the point-counting approach of stereological methods by Cavalier's principle. There was no statistically significant cerebellar atrophy or hemicerebellar asymmetry between the MWoA and control subjects. There was no cerebellar atrophy or asymmetry between the MWoA and age-matched control group. The stereological evaluation of cerebellar asymmetry and atrophy in humans is important for both clinicians and anatomists. The technique is simple, inexpensive, and reliable.
Assuntos
Cerebelo/patologia , Enxaqueca sem Aura/patologia , Feminino , Humanos , Imageamento por Ressonância Magnética , Masculino , Pessoa de Meia-Idade , Tamanho do ÓrgãoRESUMO
The human genome has been shaped by evolutionary and historical forces. Therefore, genetic polymorphisms are useful tools not only to understand the susceptibility to disease in modern populations, but the history of ancestral populations as well. For this purpose, data on genetic polymorphisms such as human leucocyte antigen, mitochondrial DNA sequence variability and the frequencies of TAP1 and TAP2 gene variants in Turkey have been reported previously. Here we have used interleukin (IL)-10 (-592C/A, -819T/C, -1082G/A) and IL-2 (-330T/G) as genetic markers to study the relationship between Turkish population and other populations.
Assuntos
Interleucina-10/genética , Interleucina-2/genética , População/genética , Adolescente , Adulto , Alelos , Frequência do Gene , Marcadores Genéticos , Genótipo , Humanos , Pessoa de Meia-Idade , Polimorfismo Genético , Turquia , Adulto JovemAssuntos
Anti-Inflamatórios/uso terapêutico , Emolientes/uso terapêutico , Lábio/patologia , Doenças da Boca/patologia , Pregnadienodiois/uso terapêutico , Psoríase/patologia , Administração Tópica , Adulto , Diagnóstico Diferencial , Feminino , Humanos , Furoato de Mometasona , Doenças da Boca/etiologia , Psoríase/etiologia , Recidiva , Resultado do TratamentoRESUMO
UNLABELLED: The effect of H. pylori infection on gastric epithelial cell apoptosis and proliferation is contradictory. Using immunohistochemistry and electron microscopy, this study sought to demonstrate gastric epithelial changes (ie, apoptosis and proliferation) due to chronic H. pylori infection. METHODS: Eighteen female 6- to 8-week old Swiss Albino mice were inoculated intragastrically with 3 doses of 10(9) CFU/mL H. pylori prepared in a Brucella Broth in 5 days. Nine others served as a control group. At the end of 28 weeks, tissue specimens from the gastric antrum were excised and examined immunohistochemically (epithelial growth factor for regeneration and Caspase-3 for apoptosis) and electron microscopically. Immunohistochemical assessment was performed using the indirect peroxidase-antiperoxidase method. RESULTS: In the H. pylori-infected group, EGF staining in gastric epithelium was found to be decreased significantly compared to that in control group (P < 0.001). Caspase-3 reactivity was commonly observed in surface epithelial cells and glandular epithelial cells in H. pylori-infected group and totally it was statistically significant compared to Caspase-3 staining in control group (P < 0.001). Electron micrograph images demonstrated numerous apoptotic cells with condensed chromatin. CONCLUSION: Chronic H. pylori infection of 28 weeks' duration increases apoptosis in gastric epithelium; however, increased apoptosis does not induce proliferation.
Assuntos
Apoptose , Proliferação de Células , Células Epiteliais/ultraestrutura , Mucosa Gástrica/ultraestrutura , Infecções por Helicobacter/fisiopatologia , Helicobacter pylori/ultraestrutura , Animais , Caspase 3 , Caspases/metabolismo , Modelos Animais de Doenças , Fator de Crescimento Epidérmico/metabolismo , Células Epiteliais/metabolismo , Células Epiteliais/virologia , Feminino , Mucosa Gástrica/citologia , Mucosa Gástrica/microbiologia , Mucosa Gástrica/patologia , Infecções por Helicobacter/metabolismo , Helicobacter pylori/metabolismo , Imuno-Histoquímica , Camundongos , Microscopia EletrônicaRESUMO
Multiple eruptive dermatofibromas (MEDF) are rare and their aetiology is unknown. More than half of the patients with MEDF have underlying diseases, and more than 80% of the underlying diseases are immune mediated. Familial cases suggesting a genetic cause have also been reported. We report a case of familial eruptive dermatofibromas associated with atopic dermatitis, possibly caused by both hereditary factors and compromised immunity.
Assuntos
Dermatite Atópica/genética , Histiocitoma Fibroso Benigno/genética , Adulto , Dermatite Atópica/imunologia , Histiocitoma Fibroso Benigno/imunologia , Humanos , Masculino , LinhagemRESUMO
Isotretinoin (13-cis-retinoic acid) is one of the synthetic retinoids derived from vitamin A. Vitamin A derivatives demonstrate virucidal activity, both in vivo and in vitro. Isotretinoin has been used for the treatment of recurrent herpes simplex with encouraging results. However, we present a case with frequent attacks of herpes labialis during isotretinoin therapy for acne, who had a marked decrease in frequency of recurrences following strict use of sunscreens.
Assuntos
Acne Vulgar/tratamento farmacológico , Herpes Labial/induzido quimicamente , Isotretinoína/efeitos adversos , Adulto , Feminino , Herpes Labial/prevenção & controle , Humanos , Recidiva , Protetores Solares/uso terapêuticoRESUMO
Idiopathic guttate hypomelanosis (IGH) is a common but often unrecognized skin disease with obscure etiopathogenesis. As the lesions mostly develop on the exposed parts of the extremities, ultraviolet has been hypothesized to induce these lesions. However, previous statistical studies did not confirm any association between ultraviolet and IGH. We describe a 72-year-old woman with mycosis fungoides in whom widespread IGH occurred during narrow band ultraviolet B (UVB) therapy. The lesion development in the trunk following narrow band UVB therapy suggests that these lesions may not be idiopathic and they may be associated with UV exposure although controversy exist in the literature. This case is particular as it reveals strong evidence on this relationship.
Assuntos
Hipopigmentação/patologia , Micose Fungoide/patologia , Neoplasias Cutâneas/patologia , Terapia Ultravioleta , Idoso , Feminino , Humanos , Hipopigmentação/etiologia , Micose Fungoide/complicações , Micose Fungoide/radioterapia , Neoplasias Cutâneas/complicações , Neoplasias Cutâneas/radioterapia , Terapia Ultravioleta/efeitos adversosRESUMO
Favre-Racouchot disease (FRD) is a common disease characterized by solar elastosis and large open comedones and cysts. This disorder mainly affects elderly Caucasian men who have had a great deal of exposure to the sun. Patients having FRD continuously develop numerous large open comedones that could cause a cosmetic problem. We performed a simple and effective extraction technique for these open comedones using a standard dissecting forceps. This technique is well tolerated and no complications were observed. We suggest this comedone extraction technique as a simple alternative treatment for this common cosmetic problem.
Assuntos
Cisto Epidérmico/terapia , Dermatoses Faciais/terapia , Idoso , Cisto Epidérmico/complicações , Cisto Epidérmico/patologia , Cisto Epidérmico/urina , Dermatoses Faciais/complicações , Dermatoses Faciais/patologia , Humanos , MasculinoRESUMO
We report a case of recurrent localized Sweet's syndrome (SS) with pulmonary sarcoidosis and hepatitis C virus infection. Hepatitis C may be the triggering factor for both Sweet's syndrome and sarcoidosis through stimulation of T helper 1 immune responses involved in the pathogenesis of both diseases.