Comprehensive insights into the effects of acidogenic off-gas utilization on successive biogas production, microbial community structure and metabolite distribution during two-stage anaerobic digestion.

Although two-stage anaerobic digestion (TSAD) technology has been investigated, the mechanisms regarding the impact of acidogenic off-gas (AOG) on successive methane production have not been well addressed. In this study, a novel TSAD system was designed. Food waste, as the main substrate, was co-digested with chicken manure and corn straw. The acidogenic gas beyond atmospheric pressure was introduced into the bottom of the methanogenesis reactor through a stainless steel diffuser. Results showed the addition of AOG increased the methane yield from 435.2 to 597.1 mL/g VSin in successive methanogenesis stage, improved by 37.2 %, and increased the energy yield from 9.0 to 11.3 kJ/g VSsubstrate. However, the theoretical contribution of hydrogenotrophic methanogenesis using H2 contained in AOG was only 15.2 % of the increased methane yield. After the addition of AOG, the decreased levels of ammonia nitrogen and butyrate indicate that the stability of the AD system was improved. The electron transfer system and co-enzyme F420 activity were enhanced; however, the decrease in acetate kinase activity indicates aceticlastic methanogenesis may have been weakened. The microbial diversity and species richness were improved by the added AOG. Methanosarcina was more competitive than Methanothermobacter, enhancing the syntrophic effect. The relative abundance of protein degradation bacteria norank_f_Anaerolineaceae and lipid degradation bacteria Syntrophomonas was increased. Metabolite analysis confirmed that the addition of AOG promoted amino acid metabolism, the biosynthesis of other secondary metabolism and lipid metabolism. The improved degradation of recalcitrant organic components (lipids and proteins) in food waste was responsible for the increased methane yield. This study provides an in-depth understanding of the impact of AOG utilization on successive methane production and has practical implications for the treatment of food waste.

Mechanical pretreatment of typical agricultural biomass on shape characterization and NO emissions during combustion.

The impact of mechanical pretreatment of corn straw (CS), pea straw (PS), and wheat straw (WS), on shape characterization and NO emissions during combustion were investigated in this research. Particle size ranges were obtained and characterized their shape factors using Image J correction. The thermal properties and NO emissions of the different-sized particles were investigated by TG-MS and fixed-bed reactor. Compared with CS and PS, WS is more likely to break into smaller particles due to its moderate strength. Amine-N completely disappeared after pyrolysis, whereas pyrrolic-N and pyridinic-N were the main N functionalities in char-N. During the volatile burning stage, the maximum peak of NO concentration was 270, 354 and 311 ppm for CS, PS and WS, respectively. NO was detected at a steady level during the semicoke combustion stage, and the duration increased with particle size. The NO concentration decreased sharply in a short duration during the fixed carbon combustion stage.

Integration of two-stage anaerobic digestion process with in situ biogas upgrading.

High impurity concentration of biogas limits its wide commercial utilization. Therefore, the integration of two-stage anaerobic digestion process with in situ biogas upgrading technologies is reviewed, with emphasis on their principles, main influencing factors, research success, and technical challenges. The crucial factors that influence these technologies are pH, alkalinity, and hydrogenotrophic methanogenesis. Hence, pH fluctuation and low gas-liquid mass transfer of H2 are some major technical challenges limiting the full-scale application of in situ upgrading techniques. Two-stage anaerobic digestion integration with various in situ upgrading techniques to form a hybrid system is proposed to overcome the constraints and systematically guide future research design and advance the development and commercialization of these techniques. This review intends to provide the current state of in situ biogas upgrading technologies and identify knowledge gaps that warrant further investigation to advance their development and practical implementation.

Insight into derivative Weibull mixture model in describing simulated distributed activation energy model and distillers dried grains with solubles pyrolysis processes.

The kinetics of biomass pyrolysis is fundamental for exploring its mechanisms and optimizing its processes, which is helpful for designing its systems. The derivative Weibull mixture model was proposed for kinetic description of the simulated distribution energy model (DAEM) processes and distillers dried grains with solubles (DDGS) pyrolysis processes. The conversion rate data of these processes at different heating rates could be accurately described by the derivative Weibull mixture model. Moreover, the proposed model could effectively smooth the noises contained in the experimental conversion rate data of DDGS pyrolysis. The derivative Weibull mixture model separated DDGS pyrolysis reactions into several individual processes, and provided some data required for further isoconversional kinetic analysis. The predicted curves from the derivative Weibull mixture model allowed us to obtain the effective activation energies of DDGS pyrolysis, which varied significantly from 170 to 330 kJ mol-1 in the conversion range between 0.1 and 0.9.

Investigation of product selectivity and kinetics of poplar sawdust catalytic pyrolysis over bi-metallic Iron-Nickel/ZSM-5 catalyst.

Py-GC/MS and thermogravimetric analysis were carried out to systematically explore product selectivity and kinetics of poplar sawdust catalytic pyrolysis over bi-metallic Fe-Ni/ZSM-5. The results showed that the Fe-Ni/ZSM-5 exhibited an additive effect on the production of monocyclic aromatic hydrocarbons compared to mono-metallic catalysts (Fe/ZSM-5 or Ni/ZSM-5). Fe-Ni/ZSM-5 further increased the yield of toluene (17.28 mg g-1), which was 41.4% and 80.9% higher than Fe/ZSM-5 and Ni/ZSM-5, respectively. According to the kinetic analysis, the average activation energy obtained from catalytic pyrolysis with Fe-Ni/ZSM-5 using the methods of Friedman, Starink, Flynn-Wall-Ozawa, and Kissinger-Akahira-Sunose was 156.19, 152.39, 154.30, and 152.11 kJ mol-1, respectively. Fe-Ni/ZSM-5 addition lowered the activation energy compared to non-catalytic pyrolysis at the conversion rate of 0.15-0.75. The overall catalytic pyrolysis process of poplar sawdust follows the diffusion and nucleation models. The thermodynamic parameters (enthalpy and entropy) showed positive and negative values, respectively, indicating non-spontaneous reactions during the catalytic pyrolysis process.

Effects of co-digestion of food waste, corn straw and chicken manure in two-stage anaerobic digestion on trace element bioavailability and microbial community composition.

Co-digestion is known to effectively alleviate trace elements (TEs) deficiency in mono-substrates; however, the bioavailability of TEs is crucial for the stability of anaerobic digestion. Therefore, this study investigated the effects of co-digestion of food waste (FW), corn straw (CS) and chicken manure (CM) in two-stage anaerobic digestion on TEs bioavailability and microbial community composition. Various VSFW:(VSCS:VSCM) ratios of 8:2, 7:3, 4:6, and 2:8 were evaluated in two-stage (group A, B, C, D) anaerobic digestion in which the VSCS:VSCM ratio was fixed at 3:1. Results showed that the highest hydrogen production of 106 mL/g VS and methane production co-efficiency of 125.3% was obtained in group A. Group A has a high close range of easily bioavailable TEs (32-64%) compared to other groups, especially the mono-substrate, where almost all TEs ranged between 10 and 36%. The increased relative abundance of the obligate hydrogenotrophic methanogens reflected a positive two-stage methane co-digestion efficiency.

Anaerobic co-digestion of corn stover and chicken manure using continuous stirred tank reactor: The effect of biochar addition and urea pretreatment.

The performance of biochar mediated anaerobic co-digestion (co-AD) of corn stover (CS) and chicken manure (CM) using continuous stirred tank reactor (CSTR) was studied. Results showed that urea pretreated CS (UPCS) and biochar addition in anaerobic digestion (AD) system can improve co-AD. The effect of urea pretreatment is similar to that of biochar addition, and their synergistic effect was apparent under medium and high OLR conditions. When the OLR was 4.2 and 6.3 g VS/L/d, the biochar mediated UPCS/CM co-AD operated stably with the VMP of 2.160 and 1.616 L/L/d, and VMP of the biochar mediated UPCS /CM were 32.8%-89.6% and 27.8%-96.4% higher than other reactors, respectively. The results reveal that urea pretreatment and biochar addition promoted AD process through strengthening the buffer capacity system established by ammonia nitrogen and volatile fatty acids and improving the degradation of lignocellulosic biomass.