Manipulation of Gut Symbionts for Improving the Sterile Insect Technique: Quality Parameters of Bactrocera dorsalis (Diptera: Tephritidae) Genetic Sexing Strain Males After Feeding on Bacteria-Enriched Diets.

One environmentally friendly method used to manage Bactrocera dorsalis (Hendel), a key agricultural pest of substantial economic importance, is the sterile insect technique (SIT). Nevertheless, several deficiencies related to this strategy impair the success of the SIT, including the inferior performance of released sterile males compared with wild males, which could be partly solved by the utilization of gut symbionts as probiotic dietary components. In this study, a culture-dependent method was used to isolate and characterize gut-associated bacterial species in adult B. dorsalis genetic sexing strain (GSS) males. In addition, three bacterial isolates from the Enterobacteriaceae family, namely, Enterobacter sp., Morganella morganii, and Moellerella wisconsensis, were used as supplements in larval and adult diets to assess their effects on the life-history traits of irradiated males. Consistent with many previous studies, Enterobacter spp. was shown to be beneficial, with some quality control indices, such as adult size, pupal weight, survival rate under stress and nutritionally rich conditions, and mating competitiveness, being significantly increased, while slight nonsignificant increases in emergence rate and flight ability were observed. Conversely, the M. morganii and M. wisconsensis strains both had negative effects on irradiated male fitness and mating competitiveness. Our results, in combination with those of earlier studies, can contribute to improving the effectiveness of SIT application by enhancing the different aspects of augmentative rearing and biological traits of pests under laboratory rearing conditions.

Life History and Host Preference of Trichopria drosophilae from Southern China, One of the Effective Pupal Parasitoids on the Drosophila Species.

This study aims to evaluate several life-history traits of a T. drosophilae population from southern China and its parasitic preference of three Drosophila species. For mated T. drosophilae females, the mean oviposition and parasitization period were 27.20 and 37.80 d, respectively. The daily mean parasitization rate was 59.24% per female and the lifetime number of emerged progeny was 134.30 per female. Trichopria drosophilae females survived 37.90 and 71.61 d under host-provided and host-deprived conditions, respectively. To assess the potential for unmated reproduction in T. drosophilae, the mean oviposition and parasitization period of unmated females was 22.90 and 47.70 d, respectively. They had a daily mean parasitization rate of 64.68%, produced a total of 114.80 offspring over their lifetime, and survived 52 d. Moreover, T. drosophilae showed a preference towards D. suzukii based on the total number of emerged offspring under a choice test. Our findings indicate that T. drosophilae from southern China appears to be suitable for the control of D. suzukii in invaded areas, due to its reproductive potential.

Evaluation of Protein Bait Manufactured From Brewery Yeast Waste for Controlling Drosophila suzukii (Diptera: Drosophilidae).

We evaluated a protein bait based on an enzymatically hydrolyzed beer yeast and two widely used baits including a sugar + vinegar + wine mixture and apple cider vinegar for their ability to trap Drosophila suzukii (Matsumura) in the laboratory and outdoors. The protein bait was a more attractive lure than the other tested baits, with the protein bait capturing significantly more female and male adults at different developmental stages than the other baits. Furthermore, the protein bait with 20% vinegar attracted significantly more adult flies than the other baits, and the protein bait without dilution attracted the most adults. Except for the addition of 0.05% spinosad, increased insecticide content in protein bait reduced its attractiveness to adult flies. Moreover, we found that D. suzukii has a bimodal activity pattern in visiting protein bait, where maximum activity occurs during 8:00-10:00 a.m. and 14:00-16:00 p.m. The antennae and maxillary palpi played an important role in detecting the chemicals emitted from the protein bait, as the number of intact flies that responded to new protein bait was significantly higher than the number of flies without antennae that responded, but almost 15% of defective flies still responded to the protein bait. Our results suggested that the protein bait based on spent brewery yeast could be a promising alternative for D. suzukii population monitoring and for managing this pest when combined with bioinsecticides, providing guidance for using this protein bait as a component of integrated pest management.