RESUMO
Striga is a root parasitic weed that attacks many of the staple crops in Africa, India and Southeast Asia, inflicting tremendous losses in yield and for which there are few effective control measures. Studies of parasitic plant virulence and host resistance will be greatly facilitated by the recent emergence of genomic resources that include extensive transcriptome sequence datasets spanning all life stages of S. hermonthica. Functional characterization of Striga genes will require detailed analyses of gene expression patterns. Quantitative real-time PCR is a powerful tool for quantifying gene expression, but correct normalization of expression levels requires identification of control genes that have stable expression across tissues and life stages. Since no S. hermonthica housekeeping genes have been established for this purpose, we evaluated the suitability of six candidate housekeeping genes across key life stages of S. hermonthica from seed conditioning to flower initiation using qRT-PCR and high-throughput cDNA sequencing. Based on gene expression analysis by qRT-PCR and RNA-Seq across heterogeneous Striga life stages, we determined that using the combination of three genes, UBQ1, PP2A and TUB1 provides the best normalization for gene expression throughout the parasitic life cycle. The housekeeping genes characterized here provide robust standards that will facilitate powerful descriptions of parasite gene expression patterns.
Assuntos
Genes Essenciais , Interações Hospedeiro-Parasita/genética , Plantas Daninhas/genética , Striga/genética , África , Sudeste Asiático , Regulação da Expressão Gênica no Desenvolvimento , Índia , Doenças das Plantas/genética , Doenças das Plantas/parasitologia , Raízes de Plantas/genética , Raízes de Plantas/crescimento & desenvolvimento , Raízes de Plantas/parasitologia , Plantas Daninhas/crescimento & desenvolvimento , RNA/genética , RNA/isolamento & purificação , Reação em Cadeia da Polimerase em Tempo Real , Análise de Sequência de RNA , Striga/crescimento & desenvolvimentoRESUMO
Parasitic plants invade host plants in order to rob them of water, minerals and nutrients. The consequences to the infected hosts can be debilitating and some of the world's most pernicious agricultural weeds are parasitic. Parasitic genera of the Scrophulariaceae and Orobanchaceae directly invade roots of neighboring plants via underground structures called haustoria. The mechanisms by which these parasites identify and associate with host plants present unsurpassed opportunities for studying chemical signaling in plant-plant interactions. Seeds of some parasites require specific host factors for efficient germination, thereby insuring the availability of an appropriate host root prior to germination. A second set of signal molecules is required to induce haustorium development and the beginning of heterotrophy. Later stages in parasitism also require the presence of host factors, although these have not yet been well characterized. Arabidopsis is being used as a model host plant to identify genetic loci associated with stimulating parasite germination, haustorium development, and parasite support. Arabidopsis is also being employed to explore how host plants respond to parasite attack. Current methodologies and recent findings in Arabidopsis - parasitic plant interactions will be discussed.
RESUMO
Parasitic plants in the Scrophulariaceae use chemicals released by host plant roots to signal developmental processes critical for heterotrophy. Haustoria, parasitic plant structures that attach to and invade host roots, develop on roots of the hemiparasitic plant Triphysaria versicolor within a few hours of exposure to either maize (Zea mays) root exudate or purified haustoria-inducing factors. We prepared a normalized, subtractive cDNA library enriched for transcripts differentially abundant in T. versicolor root tips treated with the allelopathic quinone 2,6-dimethoxybenzoquinone (DMBQ). Northern analyses estimated that about 10% of the cDNAs represent transcripts strongly up-regulated in roots exposed to DMBQ. Northern and reverse northern analyses demonstrated that most DMBQ-responsive messages were similarly up-regulated in T. versicolor roots exposed to maize root exudates. From the cDNA sequences we assembled a unigene set of 137 distinct transcripts and assigned functions by homology comparisons. Many of the proteins encoded by the transcripts are predicted to function in quinone detoxification, whereas others are more likely associated with haustorium development. The identification of genes transcriptionally regulated by haustorium-inducing factors provides a framework for dissecting genetic pathways recruited by parasitic plants during the transition to heterotrophic growth.
Assuntos
Benzoquinonas/farmacologia , Magnoliopsida/genética , DNA Complementar , DNA de Plantas , Biblioteca Gênica , Interações Hospedeiro-Parasita , Magnoliopsida/metabolismo , Magnoliopsida/parasitologia , Estruturas Vegetais/crescimento & desenvolvimento , Estruturas Vegetais/metabolismo , Análise de Sequência , Transdução de Sinais , Transcrição Gênica , Zea mays/metabolismoRESUMO
Parasitic plants in the Scrophulariaceae invade the roots of neighboring plants in order to rob them of water and nutrients. A distinctive feature of these parasites is their ability to cue their development to small molecules released by host-plant roots. Evidence is continuing to emerge that parasite perception of host factors occurs via a redox-associated mechanism. Genes predicted to function during the early stages of parasite-host interactions have been cloned from both plant partners, and their characterization is providing a genetic framework on which to model subterranean plant-plant interactions.
Assuntos
Genes de Plantas , Interações Hospedeiro-Parasita , Magnoliopsida/genética , Raízes de Plantas/parasitologia , Benzoquinonas/metabolismo , Clonagem Molecular , Regulação da Expressão Gênica de Plantas , Magnoliopsida/fisiologia , Oxirredução , Fenômenos Fisiológicos Vegetais , Raízes de Plantas/genética , Transdução de Sinais , Especificidade da EspécieRESUMO
Parasitic plants, including the root holoparasites Orobanche spp., cause devastating damage to crops worldwide. Arabidopsis thaliana (L.) is widely used an amenable model for the study of plant biology, including plant-pathogen interactions. Bringing the two plants together in a controlled system will enable the study of the molecular and genetic basis involved in host-parasitic plant interactions and should provide tools for the detection of genes responsible for incompatibility and resistance responses. The objective of this study was to screen Arabidopsis lines for reduced germination of Orobanche seeds. A 96-cell well bioassay was developed to test the potential of lines, ecotypes and mutants of Arabidopsis to induce germination of Orobanche. Screening of 50 A. thaliana ecotypes did not reveal non-inducing ecotypes. Screening of 13000 A. thaliana fast neutron mutated M2 plants detected 94 non-inducing mutant plants of which 34 were rescued, self pollinated, and M3 seeds collected. M3 seedlings from five lines were reduced in their ability to induce germination. In a separate assay, we determined that the reduced germination rates corresponded with reduced distance from the roots at which germination occurred. While further studies are necessary to determine the segregation of low germination phenotypes, these lines might prove useful for studying the genetic basis of variation in germination stimulant production in A. thaliana.
RESUMO
Haustoria are parasitic plant specific organs that locate, attach to, and invade host plant tissues. Parasitic species of the Scrophulariaceae develop haustoria on their roots in response to chemical signals released by host plant roots. Haustorium development was induced in vitro in roots of the parasitic Scrophulariaceae Triphysaria versicolor by treating them with exudates obtained from maize roots, the chemical 2,6-dimethoxybenzoquinone (DMBQ) or the cytokinin 6-benzylaminopurine (BAP). Morphological responses of T. versicolor roots to these haustoria inducing factors (HIFs) included localized swelling and epidermal hair proliferation near the root tips. These responses were not observed when roots of the non-parasitic Scrophulariaceae Lindenbergia muraria were similarly treated. Because expansin proteins are closely associated with plant cell wall expansion and growth, we examined the expression of expansin genes in response to HIFs. We isolated cDNAs homologous to transcripts encoding three distinct alpha-expansin proteins in T. versicolor. Northern-blot analyses indicated that these transcripts were differentially abundant in different tissues. Steady-state levels of two expansin transcripts increased in T. versicolor roots exposed to BAP, but not DMBQ or maize root exudates. Expansin transcript abundance also increased in L. muraria in response to BAP treatment. These results suggest that the expansins examined fulfill functions distinct from haustorium development.
Assuntos
Adenina/análogos & derivados , Magnoliopsida/genética , Proteínas de Plantas/genética , RNA de Plantas/metabolismo , Adenina/farmacologia , Sequência de Aminoácidos , Benzoquinonas/farmacologia , Compostos de Benzil , Southern Blotting , Clonagem Molecular , DNA Complementar/química , DNA Complementar/genética , DNA de Plantas/genética , Regulação da Expressão Gênica de Plantas/efeitos dos fármacos , Cinetina , Magnoliopsida/efeitos dos fármacos , Magnoliopsida/crescimento & desenvolvimento , Dados de Sequência Molecular , Isoformas de Proteínas/genética , Purinas , RNA de Plantas/efeitos dos fármacos , RNA de Plantas/genética , Alinhamento de Sequência , Análise de Sequência de DNA , Homologia de Sequência de AminoácidosRESUMO
We are using the facultative hemiparasite, Triphysaria, as a model for studying host-parasite signaling in the Scrophulariaceae. Parasitic members of this family form subterranean connections, or haustoria, on neighboring host roots to access host water and nutrients. These parasitic organs develop in response to haustorial-inducing factors contained in host root exudates. A well-characterized inducing factor, 2, 6-dimethoxy-p-benzoquinone (DMBQ), can be used to trigger in vitro haustorium formation in the roots of Triphysaria. We have assayed three species, Triphysaria eriantha (Benth.) Chuang and Heckard, Triphysaria pusilla (Benth.) Chuang and Heckard, and Triphysaria versicolor Fischer and C. Meyer, for haustorium development in response to DMBQ. There were significant differences between the species in their ability to recognize and respond to this quinone. Ninety percent of T. versicolor individuals responded, whereas only 40% of T. pusilla and less than 10% of T. eriantha formed haustoria. Within field collections of self-pollinating T. pusilla, differential responsiveness to DMBQ was seen in distinct maternal families. Assaying haustorium development in subsequent generations of self-pollinated T. pusilla showed that DMBQ responsiveness was heritable. Reciprocal crosses between T. eriantha and T. versicolor demonstrated that DMBQ responsiveness was influenced by maternal factors. These results demonstrate heritable, natural variation in the recognition of a haustorial-inducing factor by a parasitic member of the Scrophulariaceae.
Assuntos
Asteraceae/fisiologia , Benzoquinonas/farmacologia , Magnoliopsida/fisiologia , Cruzamentos Genéticos , Variação Genética , Hibridização Genética , Magnoliopsida/genética , Magnoliopsida/crescimento & desenvolvimento , Especificidade da EspécieRESUMO
Allelopathic chemicals released by plants into the rhizosphere have effects on neighboring plants ranging from phytoxicity to inducing organogenesis. The allelopathic activity of naturally occurring quinones and phenols is primarily a function of reactive radicals generated during redox cycling between quinone and hydroquinone states. We isolated cDNAs encoding two distinct quinone oxidoreductases from roots of the parasitic plant Triphysaria treated with the allelopathic quinone 2,6-dimethoxybenzoquinone (DMBQ). TvQR1 is a member of the zeta-crystallin quinone oxidoreductase family that catalyzes one-electron quinone reductions, generating free radical semiquinones. TvQR2 belongs to a family of detoxifying quinone oxidoreductases that catalyze bivalent redox reactions which avoid the radical intermediate. TvQR1 and TvQR2 message levels are rapidly upregulated in Triphysaria roots as a primary response to treatment with various allelopathic quinones. Inhibition of quinone oxidoreductase enzymatic activity with dicumarol prior to quinone treatment resulted in increased transcript levels. While TvQR2 homologs were upregulated by DMBQ in roots of all plants examined, TvQR1 homologs were upregulated only in roots of parasitic plants. Phylogenetic trees constructed of TvQR1 and TvQR2 protein homologs in Archea, Eubacteria and Eukaryotes indicated that both gene families are ancient, yet the families have dissimilar evolutionary histories in angiosperms. We hypothesize that TvQR2-like proteins function to detoxify allelopathic quinones in the rhizosphere, while TvQR1 has specific functions associated with haustorium development in parasitic plants.
Assuntos
Alelos , Plantas/enzimologia , Quinona Redutases/metabolismo , Quinonas/farmacologia , Sequência de Aminoácidos , Animais , Dados de Sequência Molecular , Filogenia , Plantas/efeitos dos fármacos , Plantas/genética , Quinona Redutases/química , Homologia de Sequência de AminoácidosRESUMO
Agricultural genomics has the potential to dramatically enrich the availability and quality of food supplies worldwide. However, because thousands of different plant species are grown for food, the application of genomics to crop improvement faces issues distinct from those in medical research. The challenge to agricultural plant scientists is to exploit the databases being generated for rice, maize, and Arabidopsis toward the genetic improvement of non-model crop species. The work in our lab illustrates one example of how genomic approaches can be applied to a non-model plant. Our overall goal is to understand how roots of different plants interact and use this information to improve the subterranean performance of crops in relation to weeds. The most obvious manifestation of root-root interactions is haustoria development. Haustoria are parasitic plant-specific organs that invade host plants and rob them of water and nutrients. Parasitic members of the Scrophulariaceae develop haustoria in vitro when exposed to molecules released by host roots. This is a useful phenotype for investigating plant-plant interactions because it is rapid, highly synchronous, and strictly dependent on exogenous haustoria-inducing factors (HIFs). Using a PCR-based subtractive hybridization, we cloned several hundred cDNAs representing transcripts one to two orders of magnitude more abundant in the roots of a parasitic plant after HIF exposure. Putative functions for about 90% of these transcripts could be assigned by searching the public databases. These have been arrayed on nylon filters and interrogated with a variety of probes from different parasitic and nonparasitic plants. Results from these experiments allowed us to identify likely candidate genes for the perception and processing of root signals by neighboring plants.
Assuntos
Genômica , Raízes de Plantas/fisiologia , Biologia Computacional , Modelos Biológicos , Plantas/genéticaRESUMO
The ability of plants to fulfill nutritional needs by parasitizing neighboring plants has originated several times in angiosperm evolution. Molecular tools are now being exploited to investigate the evolutionary origins of plant parasitism and to dissect the genetic mechanisms governing parasitic plant-host plant interactions. Investigating the nature of signal exchanges between parasitic plants and their hosts serves as a tractable system for understanding how plants in general communicate in the environment. This work should also lead to the development of novel strategies for minimizing the devastation caused by parasitic weeds in international agriculture.
Assuntos
Modelos Biológicos , Fenômenos Fisiológicos Vegetais , Plantas/parasitologia , Transdução de Sinais , Evolução Biológica , Plantas/genética , Plantas/metabolismoRESUMO
Triphysaria is a facultative root parasite in the Scrophulariaceae family. Similar to other related parasites, the development of the parasitic life cycle is initiated by molecular signals released from appropriate host roots. Using a differential display, we isolated cDNAs preferentially abundant in T. versicolor roots exposed to Trifolium repens (white clover) root exudates in vitro. Sequence analysis indicated that one of the differentially expressed cDNAs had significant homology to the nitrogen-assimilating enzyme, asparagine synthetase (AS). T. versicolor AS cDNA clones were isolated and placed into three distinct classes on the basis of nucleotide sequence variations. All three classes encoded identical AS proteins. AS was expressed in both roots and shoots of in-vitro-cultured T. versicolor. Steady-state levels of AS mRNA increased in T. versicolor roots several-fold when seedlings were exposed to exudate obtained from hydroponically grown Arabidopsis thaliana roots. Therefore, AS transcript levels increased in response to exudates from two different hosts (Trifolium and Arabidopsis). The T. versicolor AS message levels increased to a similar magnitude when seedlings were incubated in the dark. Interestingly, AS levels were unaffected by treatment with the Striga haustoria inducer 2,6-dimethoxybenzoquinone. The potential role of AS in root parasitism is discussed.
Assuntos
Aspartato-Amônia Ligase/genética , Genes de Plantas , Magnoliopsida/enzimologia , Magnoliopsida/genética , Sequência de Aminoácidos , Sequência de Bases , Primers do DNA/genética , DNA Complementar/genética , DNA Complementar/isolamento & purificação , DNA de Plantas/genética , DNA de Plantas/isolamento & purificação , Regulação Enzimológica da Expressão Gênica , Regulação da Expressão Gênica de Plantas , Interações Hospedeiro-Parasita , Magnoliopsida/parasitologia , Dados de Sequência Molecular , Raízes de Plantas/enzimologia , Raízes de Plantas/genética , Raízes de Plantas/parasitologia , RNA Mensageiro/biossíntese , RNA Mensageiro/genética , RNA de Plantas/biossíntese , RNA de Plantas/genética , Transdução de SinaisRESUMO
The site-selected insertion (SSI) procedure was used to generate insertional knockout mutations in the gene for tomato polygalacturonase (PG), a critical enzyme in fruit ripening. Previously, it had been shown that the Dissociation (Ds) elements in a select group of tomato plants frequently inserted into PG, at least in somatic tissues. DNA isolated from pollen produced by progeny of these plants was screened by SSI to identify plants likely to transmit the insertions in PG to progeny. These results identified one family as likely candidate for yielding germinally transmitted insertions. Four thousand progeny were screened and five were found containing germinally transmitted Ds insertions in PG, one of which contained two Ds insertions in PG. The Ds elements were stabilized by genetically removing the transposase and four of the five insertions were recovered as homozygous in the next generation. Enzymatic analysis of fruit from these individuals demonstrated that there was at least a 1000-fold reduction in polygalacturonase levels in those plants bearing Ds insertions in PG exons. Individuals with modified PG sequences due to the sequence footprint, resulting from excision of the element, were identified using the single-strand conformational polymorphism (SSCP) method. Enzymatic analysis of fruit from a plant homozygous for one such excision allele showed a significant reduction in polygalacturonase activity. Since there is no transgenic material left in PG, this demonstrates the ability to modify a gene of commercial value in planta and subsequently removing all transgenic material.
Assuntos
Genes de Plantas , Poligalacturonase/genética , Solanum lycopersicum/enzimologia , Solanum lycopersicum/genética , Alelos , Sequência de Bases , Primers do DNA/genética , DNA de Plantas/genética , Solanum lycopersicum/crescimento & desenvolvimento , Mutagênese Insercional , Plantas Geneticamente Modificadas , Poligalacturonase/metabolismo , Polimorfismo Conformacional de Fita SimplesRESUMO
Parasitic plants use host molecules to trigger development programs essential for parasitism. One such program governs the initiation, development, and function of haustoria, parasite-specific organs responsible for attachment and invasion of host tissues. Haustoria development can be initiated by several different molecules produced by appropriate host species. We are interested in understanding how these signals are interpreted by two related facultative parasites, Triphysaria eriantha (Benth). Chuang and Heckard, and T. versicolor Fischer and C. Meyer, to distinguish their own roots from those of potential hosts. We used an in vitro bioassay to determine what proportion of different Triphysaria populations formed haustoria in the presence and absence of closely related and unrelated host species. We found that the proportion of plants with haustoria was the same whether the plants were grown in isolation or with a conspecific host. In contrast, a significantly higher proportion of plants made haustoria when the host was a congeneric Triphysaria. Plants with haustoria neither enhanced nor inhibited other plants' propensity to form haustoria. Together these results indicate that qualitative differences exist in haustorium-inducing factors exuded by closely related species. The highest proportion of Triphysaria had haustoria when growth with Arabidopsis thaliana (L.) Heynh. Even in this case, however, some Triphysaria failed to develop haustoria. Interestingly, the percentage of haustoria that had vessel elements was higher when connections were made with Arabidopsis than with another Triphysaria. These results demonstrate that host recognition can be manifested at multiple points in haustorium development.
Assuntos
Interações Hospedeiro-Parasita , Fenômenos Fisiológicos Vegetais , Plantas/parasitologia , Arabidopsis/fisiologia , Plantas/genética , Especificidade da Espécie , Fatores de TempoRESUMO
Site-selected insertion (SSI) is a PCR-based technique which uses primers located within the transposon and a target gene for detection of transposon insertions into cloned genes. We screened tomato plants bearing single or multiple copies of maize Ac or Ds transposable elements for somatic insertions at one close-range target and two long-range targets. Eight close-range Ds insertions near the right border of the T-DNA were recovered. Sequence analysis showed a precise junction between the transposon and the target for all insertions. Two insertions in separate plants occurred at the same site, but others appeared dispersed in the region of the right T-DNA border with no target specificity. However, insertions showed a preference for one orientation of the transposon. Use of plants with multiple Ac (HiAc) or Ds (HiDs) elements allowed detection of somatic insertions at two single-copy genes, PG (polygalacturonase) and DFR (dihydroflavonol 4-reductase). Certain HiDs plants showed much higher rates of insertion into PG than others. Insertions in PG and DFR were found throughout the gene regions monitored and, with the exception of one insertion in PG, the junctions between transposon and target were exact. SSI analysis of progeny from the HiDs parents revealed that in some cases the tendency to incur high levels of somatic insertions in PG was inherited. Inheritance of this character is an indication that SSI could be used to direct a search for germinal PG insertions in tomato.
Assuntos
Elementos de DNA Transponíveis/genética , Mutagênese Insercional , Solanum lycopersicum/genética , Zea mays/genética , Oxirredutases do Álcool/genética , Sequência de Bases , Southern Blotting , Mapeamento Cromossômico , Clonagem Molecular , Primers do DNA , DNA Bacteriano/genética , Genes de Plantas/genética , Dados de Sequência Molecular , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Poligalacturonase/genéticaRESUMO
We have isolated a novel mutation that caused variegated leaf color in a tomato plant which had multiple maize Ac transposable elements and the tomato Xa allele. Xa is a previously characterized semi-dominant mutation that causes tomato leaves to be bright yellow when heterozygous (Xa/xa+). The mutation responsible for the new phenotype was named Mox (Modifier of Xa). The Mox mutation modified the Xa/xa+ yellow leaf phenotype in two ways: it compensated for the Xa allele resulting in a plant with a wildtype green color, and it caused somatic variegation which appeared as white and yellow sectors on the green background. Somatic variegation was visible only if the plant contained both the Mox and Xa loci. Genetic studies indicated that the Mox locus was linked in repulsion to Xa and that the Mox locus was genetically transmitted at a reduced frequency through the male gamete. Molecular characterization of the Ac elements in lines segregating for Mox identified an Ac insertion that appeared to cosegregate with Mox variegation. We propose a model in which the Mox mutation consists of a duplication of the xa+ allele and subsequent Ac-induced breakage of the duplicated region causes variegation.
Assuntos
Genes de Plantas , Solanum lycopersicum/genética , Alelos , Elementos de DNA Transponíveis , Ligação Genética , Mutação , Pigmentação/genética , Zea mays/genéticaRESUMO
We have assayed the transposition activity of the maize transposable element Ac in transgenic tomato plants that had a single copy of Ac. We found that Ac elements were in either a high or low activity state and that an Ac insertion could cycle from low to high activity within a generation. The different transposition activities were not simply due to the chromosomal position of the element, because the same Ac insertion had different levels of activity in sibling plants. Transposition activity was measured by two methods, one genetic and one physical; both assays gave similar results for each plant studied. Notably, plants with active Ac elements had progeny with amplified Ac copy number, while no amplification was detected in lines containing Ac in a low activity state. Analysis of lines with amplified elements revealed that the elements could be either clustered or dispersed. Our results were consistent with amplification being the result of transposition.
Assuntos
Elementos de DNA Transponíveis/fisiologia , Solanum lycopersicum/genética , Zea mays/genética , DNA de Plantas/genética , Amplificação de Genes , Metilação , Plantas Geneticamente Modificadas , Recombinação GenéticaRESUMO
We are examining the behavior of the maize transposable element Ac in transgenic tomato with the goal of developing an efficient insertional mutagenesis system. Among the self progeny of a transgenic tomato plant containing an active Ac element, we identified six plants that contained the same germinally transposed Ac. In one of these plants, we found a second Ac element inserted in the same orientation and approximately 2 kb to the 5′ side of the original Ac insertion. Transmission of this composite structure was significantly reduced with less than one-quarter of the self progeny inheriting Ac either in the form of the intact parental allele (two neighboring Ac's) or derivatives of it. The derivative alleles that arose were complex in structure and could not be explained solely on the basis of the excision of one or the other Ac element. These results illustrate the potential of transposable elements to cause genetic instabilities and complex chromosomal rearrangements.
RESUMO
We isolated the dihydroflavonol 4-reductase (DFR) gene from tomato (Lycopersicon esculentum) using a previously characterized cDNA as probe. Earlier studies had indicated that the DFR gene is present in tomato as a single gene located on chromosome 2 near the locus anthocyanin without (aw). Mutant alleles of the aw locus result in the complete absence of anthocyanin pigmentation throughout all stages of plant development. When the genomic DFR clone was introduced by Agrobacterium-mediated transformation into plants bearing the aw mutation, primary transgenic seedlings accumulated anthocyanins that could be observed while the plants were still in tissue culture and which continued to be observed as the plants matured. Progeny of self pollinated and backcrossed transgenic plants segregated for anthocyanin pigmentation, and Southern hybridization analyses indicated the presence of the DFR transgene exclusively in those plants with pigmentation. These data indicate that the aw locus likely corresponds to the structural gene for DFR and that DFR can be used as a visual, nondestructive, plant-derived marker gene for tomato.
RESUMO
A cDNA clone (DFR) encoding dihydroflavonol 4-reductase was identified from tomato hypocotyls. Nucleotide and amino acid sequence comparisons to Petunia hybrida, Antirrhinum majus and Zea mays DFR sequences confirmed that the cDNA encodes the structural DFR gene. In tomato, the DFR sequence appeared to be present as a single gene and mapped to a region on chromosome 2 near two loci affecting anthocyanin pigmentation, are and aw. DFR was expressed in both leaf and hypocotyl tissue. Sequencing data from two DFR cDNA clones indicated there are alternative polyadenylation sites on DFR.