Analysis on the treatment with Jiaji points / 国际中医中药杂志

Jiaji points have been widely used in the treatment of cerebral palsy, spinal cord injury, stroke, cervical spondylosis, lumbar disc herniation, ankylosing spondylitis, herpes zoster and other diseases, and shown good curative effect. The treatment methods for Jiaji points are rich, including acupuncture, electroacupuncture, moxibustion, percussion, fire needle, acupoint injection, warm acupuncture, and bloodletting. And acupuncture and electroacupuncture are the most application treatment methods. But the treatment shows narrow, scope of diseases, like internal medicine, pediatrics, and gynecology. Such treatment of Jiaji points are seldomly combined with other acupoints. Though Jiaji point treatment has good curative effect for insomnia, depression, uterine fibroids and other diseases, clinical application is few.

Different longitudinal patterns of nucleic acid and serology testing results based on disease severity of COVID-19 patients.

Effective strategy to mitigate the ongoing pandemic of 2019 novel coronavirus (COVID-19) require a comprehensive understanding of humoral responses against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the emerging virus causing COVID-19. The dynamic profile of viral replication and shedding along with viral antigen specific antibody responses among COVID-19 patients started to be reported but there is no consensus on their patterns. Here, we conducted a serial investigation on 21 individuals infected with SARS-CoV-2 in two medical centres from Jiangsu Province, including 11 non-severe COVID-19 patients, and 5 severe COVID-19 patients and 5 asymptomatic carriers based on nucleic acid test and clinical symptoms. The longitudinal swab samples and sera were collected from these people for viral RNA testing and antibody responses, respectively. Our data revealed different pattern of seroconversion among these groups. All 11 non-severe COVID-19 patients and 5 severe COVID-19 patients were seroconverted during hospitalization or follow-up period, suggesting that serological testing is a complementary assay to nucleic acid test for those symptomatic COVID-19 patients. Of note, immediate antibody responses were identified among severe cases, compared to non-severe cases. On the other hand, only one were seroconverted for asymptomatic carriers. The SARS-CoV-2 specific antibody responses were well-maintained during the observation period. Such information is of immediate relevance and would assist COVID-19 clinical diagnosis, prognosis and vaccine design.

Analysis on acupuncture and moxibustion in history / 中国针灸

school of acupuncture and moxibustion is the important component of the medicine of school, especially medical scholars. Through collecting and digging the medical books and literature of medical scholars in dynasties, the contribution to acupuncture academic development in Shandong region was introduced briefly so as to provide the references to the study on the regionalism of acupuncture school. The results showed that there was a long history of acupuncture from the Dynasty to the present and many medical scholars and medical books were sprung up. The systematic analysis on medical scholars, acupuncture works and literature as well as academic thoughts are conductive to the great-leap-forward development of school of acupuncture and moxibustion.

's academic characteristics and achievements on acupuncture / 中国针灸

, the famous medical scholar of the Northern Song Dynasty, compiled (, hereinafter referred to as ) based on the acupuncture achievements before the Song Dynasty. In reference to the records in , two acupuncture bronze figures were made and all of the text was inscribed on the stele. 's academic characteristics on acupuncture are reflected through these three different forms, including 4 aspects. ① Establishing the supplementation and classification of the numbers of acupoints; ② elaborating the indications of meridians and application of acupoints; ③ stressing the contraindication and nursing of prognosis; ④ revising acupuncture atlas and bone proportional measurement. , the bronze figure and the stele represent the eminent academic achievements of acupuncture in the Song Dynasty and have become the treasure of acupuncture history in China.

Effects of Herb-partition Moxibustion at Navel on Protein and mRNA Expressions of Bcl-2 and Bax in Rats Model with Premature Ovarian Failure / 中国中医药信息杂志

Objective To observe the effects of herb-partition moxibustion at navel on the expressions of Bcl-2 and Bax in rats with premature ovarian failure (POF); To explore its mechanism underlying improvement of POF. Methods POF model was established by intraperitoneal injection of cyclophosphamide. Forty-eight Female Wistar rats were randomly divided into normal group, model group, moxibustion group and Western medicine group, with 12 rats in each group. Rats of moxibustion group were treated with herb-partition moxibustion at navel (Shenque, CV8), 30 min/d, once every other day; rats of Western medicine group were given intragastric dose of estradiol valerate, once a day, for 14 d. The protein and mRNA expressions of Bax and Bcl-2 in ovarian granulosa cells were detected by immunohistochemal method, Western blot and qRT-PCR, respectively.Results The weights of ovaries in the model group rats were significantly lower than those in the normal group (P<0.05). The expressions of Bcl-2 and Bax protein in ovarian tissue of each group were mainly expressed brown coloration and in the cytoplasm of cells. The range of Bax protein in granulosa cells of moxibustion group and Western medicine group was significantly less than that in model group. The range of Bcl-2 in moxibustion group and Western medicine group was significantly larger than that in model group. Compared with the normal group, the expressions of Bcl-2 protein and mRNA in the model group rats significantly decreased (P<0.05,P<0.01), and the expression of Bax protein and mRNA significantly increased (P<0.05,P<0.01). The expression level of Bcl-2 protein and mRNA was significantly higher in the moxibustion group and in the western medicine group than that in the model group (P<0.05,P<0.01). The expression level of Bax protein and mRNA in the moxibustion group was significantly lower than that in the model group (P<0.05,P<0.01). ConclusionThe herb-partition moxibustion at navel can improve the function of ovaries by up regulating the expression of Bcl-2 and down regulating the expression of Bax in POF rats model.

Research Advances in the Mechanism of Acupuncture Treatment for Premature Ovarian Failure in Recent 10 Years / 上海针灸杂志

The CNKI database was searched using the keywords premature ovarian failure and acupuncture. Ninety-eight related articles published from 2006 to 2015 were retrieved and 17 with randomized controlled trials were sifted out. The recent 10 years' research situation of the mechanism of acupuncture treatment for premature ovarian failure was systematically summarized through literature review and collation. A retrospective summary was made from five aspects: improvement in ovarian endocrine function, acceleration of ovulation, effect on immunologic system, improvement in ovarian reserve function and regulation of ovarian microenvironment. It is put forward on this basis that future studies on the mechanism of acupuncture treatment for premature ovarian failure can be further into higher gene and protein levels; and no matter what kind of acupuncture manipulation is used, arrival of qi is necessary; only arrival of qi can produce an effect.

Research Advances in the Mechanism of Acupuncture and Moxibustuion Treatment for Lumbar Intervertebral Disc Herniation / 上海针灸杂志

By sorting out 2005-2015's literature on the mechanism of acupuncture and moxibustuion treatment for lumbar intervertebral disc herniation, this article concludes that the studies focused mainly on six aspects: improvement in nail fold microcirculation, improvement in neural ultrastructure, regulation of bodily autoimmunity, improvement in hemorheological indicators, regulation of neuroelectrophysiology and regulation of chemical neuroinflamma- tory mediators. A summary is made from the six aspects.

Unique Features of Acupuncture-moxibustion in Chuan Ya Wai Bian / 上海针灸杂志

Chuan Ya Wai Bian (External Therapies of Folk Medicine), compiled by ZHAO Xue-min, a medical expert in Qing Dynasty, is abundant in folk external therapies including acupuncture, moxibustion, acupoint sticking therapies, which all have unique features. Folk acupuncture-moxibustion is an important part of acupuncture- moxibustion therapy, playing a role that can't be replaced by conventional acupuncture-moxibustion. It's worth exploring and needs constant improvements to adapt to the requirement of modern medicine, which is also significant for boosting the development of acupuncture-moxibustion therapy.

Application of self-developed moxibustion thermometer in experiment teaching / 中国针灸

In order to improve the teaching quality of moxibustion experiment, a moxibustion thermometer was self-developed to monitor the real-time and continuous data of moxibustion temperature at different time points during the experiment. After teacher's explanation and demonstration of experiment process, the students used the moxibustion thermometer to monitor the change of temperature data and extended the experiment design. In the process of experiment class, the students found the temperature of the object tested increased rapidly, arrived at the highest temperature and slowly reduced. In addition, with learned knowledge, the students were able to design the feasible experiment scheme. The self-developed moxibustion thermometer operates smoothly in actual teaching, with stable experiment data and less experiment error, which obtained satisfactory teaching effect.

Analysis for contemporary academic study of / 中国针灸

is a profound book byfor acupuncture-moxibustion development. Twenty-five articles about arranging the book were analyzed in China National Knowledge Infrastructure(CNKI) from 1980 to 2015. It is considered that the versions spread are clear, and literature quoted is abundant but needed to be further sorted. Also, academic thoughts with value were deeply explored in terms of acupoint, moxibustion, acupuncture-moxibustion case, contraindication and health preserving. However, shortages about contemporary research exist, such as less exploration on historical cultural background of the book, the humanistic spirit of, the rule of acupoint application and the treatment experie-nce. We should pay attention to the above points so as to further systematically study its academic thought.

Analysis on Treatment of Oculopathy in“Acupuncture DaCheng” / 浙江中医药大学学报

Objective]Through finishing the information about oculopathy in“Acupuncture DaCheng”, analyze the treatment regulations, provide guidance for oculopathy treatment. [Method] Finishing the clause about oculopathy in“Acupuncture DaCheng”,counting numbers of points, frequency of each points, analyzing the characteristics of points, calculated out all points and the channel attribution. [Results] It is found that treatment of oculopathy inacupuncture Dachengof less points, commonly uses the extraordinary points, local points and meridians around eyes, and the characteristic of the dialectical treatment, in acupuncture treatment method at the same time attaches great importance to the use of moxibustion, and puts forward the whole set of gold needle couching. [Conclusion]Summing up the experiences of acupuncture treatment of oculopathy in“Dacheng”can provide reference for modern acupuncture of oculopathy in selection of points and ideal of treatment.

Application of problem-based learning in teaching practice of Science of Meridians and Acupoints / 中国针灸

Science of Meridians and Acupoints is the bridge between basic medicine and clinical medicine of acupuncture and moxibustion. This teaching practice was conducted in reference to the teaching mode of problembased learning (PBL), in association with the clinical design problems, by taking as the students as the role and guided by teachers. In order to stimulate students' active learning enthusiasm, the writers implemented the class teaching in views of the typical questions of clinical design, presentation of study group, emphasis on drawing meridian running courses and acupoint locations, summarization and analysis, as well as comprehensive evaluation so that the comprehensive innovative ability of students and the teaching quality could be improved.

Ancient LiteratureAnalysis of Point Shenmai Indications and the Characteristics of Acupuncture and Moxibustion / 上海针灸杂志

ObjectiveTo summarize point Shenmai(BL62)indications and the characteristics of acupuncture and moxibustion by sorting out literature on point Shenmaiin ancient literature.MethodShenmai, Yangqiao and Guilu were used as search words to search Chinese Medical Classics to sift out items on indications for point Shenmai and its combination and the characteristics of point combination and acupuncture and moxibustion and establish the relevant database. Statistical analysis was made using the SPSS19.0 software.ResultTwo hundred and eighty-seven items on point Shenmai and 274 items on the indications met the inclusion criteria. There were 47 indications for the single acupoint and 19 kinds of diseases on which the single acupoint had a marked therapeutic effect. Lumbago, ophthalmalgia, vertigo, headache and mania were ranked in the top five. There were 64 indications for its combination and 22 kinds of diseases on which its combination had a marked therapeutic effect. Epilepsy, Lumbago, stroke, podalgia and headache were ranked in the top five. The commonly used depth of needle insertion was 3 fen, acupuncture time was 6-7 respiratory cycles and stimulation intensity of moxibustion was 3 moxa cones.ConclusionSingle point Shenmai and its combination are both good at treating internal medicaldiseases and especially meridian diseases in the limb. Acupuncture and moxibustion are the commonly used methods.

Analysis on the ancient clinical application of Taiyuan (LU 9) / 中国针灸

The ancient literature on Taiyuan (LU 9) was collected to summarize the application laws of this acupoint. One thousand TCM ancient works collected in Chinese Medical Dictionary (fourth edition) were retrieved. The literature on the indications of Taiyuan (LU 9), the indications of Taiyuan (LU 9) combined prescriptions, the laws of acupoints combination and the techniques of acupuncture and moxibustion was collected systematically and the database was set up. It is found that there are 333 provisions in compliance with the inclusive standard of Taiyuan (LU 9), 306 provisions for the treatment of disorders and 66 relevant ancient works. There are 119 provisions relevant with the indication of single Taiyuan (LU 9) , 187 provisions for the applications of the combined prescriptions and 143 acupoints for the combined acupoints. Taiyuan (LU 9) and the combined prescriptions are applied to every department in clinic, especially for the disorders of TCM internal medicine department. The combined acupoints are distributed among the fourteen meridians and extra points and had been used totally for 1142 times. Of these points, there are 9 acupoints from the lung meridian of hand-taiyin and 25 acupoints from the bladder meridian of foot-taiyang, with the highest use frequency, 195 times and 141 times respectively, accounting for 17.08% and 12.35% of the total frequency. The common pairs of combination are Taiyuan (LU 9) and Yuji (LU 10), Taiyuan (LU 9) and Lieque (LU 7) and Taiyuan (LU 9) and Taibai (SP 3). The common dose of moxibustion at the acupoint is 3 cones. The depth of insertion is 0.2 cun and the acupuncture time is two breathes.

Preparation and identification of anti-HIV-1 gp120 monoclonal antibody / 中国生化药物杂志

Purpose To Prepare anti- HIV-1 gp120 monoclonal antibodies and to identify the specificity of antibodies in order to provide technique for preparing HIV remedial antibodies. Methods The gene fragment of HIV-1 gp120 was connected to PEGX-4T-2 prokaryotic expressing vector. The vector was cut by enzyme. GST-HIV protein was expressed by E. coli XL1-blue. The BALB/c mice were immunized with purified GST-HIV antigen, and then the fusion of mice spleen cell and myeloma cell SP2/0 was executed as the routine cell-fusion technique. Positive cells were screened by Indirect ELISA. Immuno-blotting assay and Western blot identified the specificity of antibodies. Results External gene section from the recombinant plasmid by sequencing showed the same size of HIV-1 gp120 gene sequences. An external expressed protein band of 32 KD was obtained after purified protein SDS-PAGE electrophoresis. It indicated that six strains of hybridoma cells secreting special monoclone antibodies had been obtained. ELISA results showed that six strains monoclonal antibodies only reacted to HIV-1 gp120 antigen. Western blot results showed that a band with molecular weight 32 kDa was obtained, which could interact with HIV-1 gp120 monoclonal antibody. Conclusion Six strains of hybridoma cells secreting special monoclone antibodies had been obtained. The prepared monoclonal antibodies have established a basis for HIV remedial antibody.

Establishment of ELISA for detection of hepatoma specific γ-glutamyltransferase and its clinical application / 中华检验医学杂志

Objective To prepare monoclonal antibody (McAb) against γ-glutamyhransferase(GGT) firmly bound to datura stramonim (DSA) leetin from primary hepatic carcinoma (PHC) tissue and establish an avidin-biotin enzyme-linked immunosorbent assay (ELISA) for evaluating the diagnostic value of serum DSA-GGT for PHC. Methods Anti-DSA-GGT monoclonal antibodies were obtained by McAb technology and purified by protein G-sepharose affinity chromatography. The McAb was labeled with biotin and avidin-biotin ELISA for measurement of serum DSA-GGT was established. Using the avidin-biotin ELISA, serum DSA-GGT levels was detected in 39 patients with PHC, and 122 patients with non-PHC diseases. The distribution of serum DSA-GGT values of 119 healthy subjects were determined by P-P plots. Optimal cut-off value for the diagnosis of PHC was determined by receiver operating characterstic (ROC) curve. Results The protein levels of McAb in the ascites derived from 5 McAb hybridoma cell strains ranged from 2. 12 to 6. 70 mg, The biotin-labeled rate varied from 48. 6% to 72. 2% respectively. The minimum detection limit of serum DSA-GGT in avidin-biotin ELISA was 2 μg/L. Intra-assay and inter-assay coefficients of variation were 8.9% and 11.5% respectively. The distribution of DSA-GGT values of 119 healthy subjects showed Gaussian distribution and its Mean ± SD was ( 1.50±0. 51 ) μg/L. Optimal cut-off value (3.25 μg/L) in the diagnosis of PHC was determined by ROC curve. DSA-GGT was positive in 26 out of 39 patients with PHC and 10 out of 122 patients with non-PHC diseases were positive. The sensitivity and specificity of this assay for the diagnosis of PHC were 66. 7% and 91.8% respectively. Conclusions The convenient avidin-biotin ELISA method was successfully established in our laboratory and it showed a good reproducibility and reliability. It may be a potential tool in the diagnosis of PHC to achieve higher sensitivity and specificity.

Role of human interferon α-2b as an immunological adjuvant for human papillomavirus (HPV) 11-E7 DNA vaccine / 中华皮肤科杂志

ngthen cellular immunity, especially Th1-type immune response to HPV11-E7 DNA vaccine in mice.

Discussion on Compatibility of Acupoints in the Book of Nanjing / 国际中医中药杂志

<Nanjing> is a famous ancient medical book of China. This article made a deep analysis on compatibility of acupoints in this book from four aspects: ① Acupoints compatibility of mother-son reinforcing-reducing method; ②Acupoints compatibility of reducing heart-fire and reinforcing kendy-watre method; ③Acupoints compatibility of needling jing-point reducing xing-point method, and ④ Acupoints compatibility of four-seasions wu-zang and wushu-point method.

Treatment of 40 Cases of Post-apoplectic Dysphagia by Acupuncture plus Rehabilitation Exercise / 针灸推拿医学（英文版）

To investigate the efficacy of acupuncture plus rehabilitation exercise in treating post-apoplectic dysphagia. Methods:The patients were randomly allocated into treatment and control groups. The treatment group was treated by acupuncturing points Fengchi(GB 20),Tianzhu(BL 10),Tongli(HT 5) and Lianquan(CV 23) plus rehabilitation exercises and the control group only by rehabilitation exercise. Results:The total effective rate was 95.0% in the treatment group and 71.9% in the control group. There was a significant difference between the two groups (P＜0.01). Conclusion:Acupuncture plus rehabilitation exercise can improve the curative effect on postapoplectic dysphagia.