Clinical analysis of Chlamydia psittaci pneumonia in the elderly / 中华全科医师杂志

Objective:To analyze the clinical characteristics of Chlamydia psittaci pneumonia in the elderly, and factor related to the disease severity. Methods:Clinical data of 32 elderly patients with Chlamydia pneumoniae pneumonia admitted in the First People′s Hospital of Xiaoshan District from January 2019 to January 2021 were retrospectively analyzed. The patients were diagnosed by the second generation sequencing using bronchoalveolar lavage fluid (BALF) samples. There were 17 patients in moderate group and 15 patients in severe group; the liver function, muscle enzymes, imaging and lymphocyte subsets of the two groups were compared. Results:There were no significant differences in the age, gender and basic diseases, bird contact history, flaccid fever and dry cough symptoms between two groups, while there were significant differences in mental symptoms and gastrointestinal symptoms between the two groups ( P=0.032, 0.018). There were significant differences in ALT , AST, LDH , CK , CK-MB, CRP, BNP , troponin-T and PCT between the two groups (all P<0.001). The increase of leukocytes was not significant in both groups. The moderate group was dominated by single lobe involvement, while the severe group was dominated by multi lobe involvement and pleural effusion ( P=0.043, 0.015, 0.023). The total lymphocytes, T lymphocytes, CD4 + T cells, CD8 + T cells, CD4 +/CD8 + ratio, NK cells, B cells, CD4 +CD8 + double positive T cells decreased in both groups, while those in the severe group decrease more markedly ( P<0.05). CD4 -CD8 - double negative T cells were higher in the severe group than those in the moderate group ( P<0.001). CD4 +/CD8 + ratio and CD4 -CD8 - double negative T cells were correlated with severity index PSI and CURB-65 ( P<0.05). Conclusions:The liver function, muscle enzyme, lymphocyte immune function in patients with Chlamydia psittaci pneumonia are impaired, which were more markedly in severe patients. The multileaf infiltration and increased procalcitonin may indicate the severe pneumonia. CD4 +/CD8 + ratio and CD4 -CD8 - double negative T cells are correlated with the severity of pneumonia.

Chemotherapeutic drugs affect methylation of ER-α in breast cancer cells by down-regulating miR-148b / 中华内分泌外科杂志

Objective To investigate the effects of chemotherapeutic drugs on ER-α expression and methylation in breast cancer cells.Methods Human breast cancer cells MCF-7(ER+,Luminal A) were induced by paclitaxel(PTX) and epirubicin(EPI) for more than 6 months,with an incremental dose,respectively.The expression and methylation status of ER-α in MCF-7 cells were detected before and after drug treatment.miRNAs with consistent expression changes in MCF-7 cells after two drugs' treatment were screened by microarray,and verified by quantitative PCR (qPCR).Targets of the most significantly down-regulated miRNA were analyzed by bioinformatics.miRNA inhibitor was transfected into MCF-7 cells,miRNA mimic was transfected into MCF-7/PTX and MCF-7/EPI cells,then ER-α and DNA methyltransferase 1 (DNMT1) expression were detected by Western blot,and ER-α methylation was detected by quantitative methylation-specific PCR (qMSP).Results PTX resistant MCF-7/PTX cell line and EPI resistant MCF-7/EPI cell line were established.Both drug treatments caused a decrease in ER-α protein expression and an increase in methylation levels,with up-regulation of DNMT1 and his tone deacetylase 1 (HDAC 1) expression.miRNAs with consistent expression changes in MCF-7 cells after drug treatments were screened and verified by qPCR,the most significant down-regulation among which was miR-148b.Bioinformatics analysis,and further confirmed by luciferase reporter gene assay (Luciferas) that DNMT1 was a direct target of miR-148b.miR-148b inhibitor induced decreased expression of ER-α and increased methylation level in MCF-7 cells,accompanied by increased expression of DNMT1;whereas miR-148b mimic caused an increased expression of ER-α and decreased methylation level in MCF-7/PTX and MCF-7/EPI cells,with a decreased expression of DNMT1.Conclusion Chemotherapeutic drugs (represented by PTX and EPI) induce aberrant miRNA expression in breast cancer MCF-7 cells,and down-regulate miR-148b further to attenuate the inhibition of DNMT1 expression,which promote,hypermethylation and down-regulation of ER-α.

Clinical observation of bronchial artery embolization for massive hemoptysis / 中国基层医药

Objective To evaluate the clinical efficacy of bronchial artery embolization in the treatment of massive hemoptysis ,and to evaluate the clinical efficacy and adverse reactions of 3-year recurrence and survival . Methods 80 patients with hemoptysis or chronic recurrent hemoptysis were selected as the research subjects , and they were randomly divided into two gourps according to the digital table ,40cases in each group.The interventional embolization group was given bronchial artery embolization .The drug group was treated with phentolamine and pituitrin.The index of the system were observed and evaluated:(1)the effect of controlling massive hemoptysis;(2) the adverse reaction of the patients;(3) the recurrence rate of 3 years.Results (1) The total effective rate of the interventional embolization group was 92.5％,which was significantly higher than 77.5％ of the drug group (χ2 =9.044,P<0.05);(2) The incidence rate of adverse reaction of the interventional embolization group was 12.5％, which was lower than 35.0％of the drug group ,the difference was not statistically significant between the two groups (χ2 =1.742,P>0.05).(3) The 3 years recurrence rate of the interventional embolization group was 7.5％,which of the drug group was 40.0％,the difference between the two groups was statistically significant (χ2 =12.557,P<0.05).The 3 years survival rate of the interventional embolization group was 95.0％,which of the drug group was 92.5％,the difference between the two groups was not statistically significant (χ2 =0.215,P>0.05).Conclusion The bronchial artery embolization in the treatment of hemoptysis has ideal clinical effect .It is a minimally invasive and effective clinical treatment method ,and the effect has safety ,less adverse reaction and low recurrence rate ,which is worthy of extensive promotion and application .

Correlation of expressions of phosphorylated collapsin response mediator protein 2 and Ⅲβ tubulin protein with chemotherapeutic efficacy in elderly patients with non-small cell lung cancer / 中华老年医学杂志

Objective To investigate the expressions of phosphorylated collapsin response mediator protein 2(pCRMP2)and class Ⅲ β-tubulin protein(Ⅲ β tubulin)in elderly patients with stage Ⅲ/Ⅳ non-small cell lung cancer (NSCLC),and to evaluate the value of the expressions of pCRMP2 and Ⅲβ tubulin in predicting the chemotherapeutic sensitivity of taxol.Methods A total of 127 elderly patients with stage Ⅲ/Ⅳ NSCLC were recruited,and expression levels of pCRMP2 and Ⅲ β tubulin were detected by immunohistochemistry and Western blot.Based on the levels of pCRMP2 and Ⅲ β tubulin expression,all patients were divided into four groups,including group A(low expressions of both pCRMP2 and lβ tubulin),group B(high expressions of both pCRMP2 and Ⅲβ tubulin),group C(high expression of only pCRMP2)and group D(high expression of only Ⅲ β tubulin).All patients were assigned to receive two cycles of TP chemotherapy regimens and then evaluate the effect of chemotherapy.If chemotherapy was invalid,a second-line chemotherapy regimen was administered.Relationships of response rate,overall survival(OS)and time on tumor progression(TTP) with expression levels of pCRMP2 and Ⅲ β tubulin protein were assessed.Results Among 127 elderly patients,the high expression rate of pCRMP2 and Ⅲ β tubulin protein were 51.2％ (65 cases) and 47.2％ (60 cases),respectively.The Western blot result displayed that the high expressions of pCRMP2 and Ⅲ β tubulin protein were significantly associated with pathologic stage,and with poorly differentiated adenocarcinoma(all P＜0.05).The pCRMP2 expression was also related to the clinical stage.Meanwhile,the overall survival rate(OS)was significantly lower in patients with high expression of pCRMP2 and Ⅲ β tubulin protein than in patients with low expression of the two proteins(P＜ 0.05).Expression of pCRMP2 and Ⅲβ tubulin protein was related with pathological grade and tissue classification.The remission rate (RR)was significantly higher in group A(65.3 ％)than in other three groups(RR in group C,group D and group B was 48.1％,44.0％,30.8％;all P＜0.05,respectively).There were significant differences in RR between group C,group D and group B(48.1 ％,44.0％,30.8％ respectively,P＜0.05).The OS of four groups were(510.5 ±20.9)d,(215.7±31.5)d,(326.9 ± 25.7)d,(298.7±21.2)d respectively.The OS was significantly longer in group A than in other groups(P ＜ 0.05).Conclusions The expression of pCRMP2 and Ⅲ β tubulin protein in NSCLC can be used to judge prognosis of lung cancer and to predict chemotherapy drug resistance.

Correlation of expressions of phosphorylated collapsin response mediator protein 2 and Ⅲβ tubulin protein with chemotherapeutic efficacy in elderly patients with non-small cell lung cancer / 中华老年医学杂志

Objective To investigate the expressions of phosphorylated collapsin response mediator protein 2(pCRMP2)and class Ⅲ β-tubulin protein(Ⅲ β tubulin)in elderly patients with stage Ⅲ/Ⅳ non-small cell lung cancer (NSCLC),and to evaluate the value of the expressions of pCRMP2 and Ⅲβ tubulin in predicting the chemotherapeutic sensitivity of taxol.Methods A total of 127 elderly patients with stage Ⅲ/Ⅳ NSCLC were recruited,and expression levels of pCRMP2 and Ⅲ β tubulin were detected by immunohistochemistry and Western blot.Based on the levels of pCRMP2 and Ⅲ β tubulin expression,all patients were divided into four groups,including group A(low expressions of both pCRMP2 and lβ tubulin),group B(high expressions of both pCRMP2 and Ⅲβ tubulin),group C(high expression of only pCRMP2)and group D(high expression of only Ⅲ β tubulin).All patients were assigned to receive two cycles of TP chemotherapy regimens and then evaluate the effect of chemotherapy.If chemotherapy was invalid,a second-line chemotherapy regimen was administered.Relationships of response rate,overall survival(OS)and time on tumor progression(TTP) with expression levels of pCRMP2 and Ⅲ β tubulin protein were assessed.Results Among 127 elderly patients,the high expression rate of pCRMP2 and Ⅲ β tubulin protein were 51.2％ (65 cases) and 47.2％ (60 cases),respectively.The Western blot result displayed that the high expressions of pCRMP2 and Ⅲ β tubulin protein were significantly associated with pathologic stage,and with poorly differentiated adenocarcinoma(all P＜0.05).The pCRMP2 expression was also related to the clinical stage.Meanwhile,the overall survival rate(OS)was significantly lower in patients with high expression of pCRMP2 and Ⅲ β tubulin protein than in patients with low expression of the two proteins(P＜ 0.05).Expression of pCRMP2 and Ⅲβ tubulin protein was related with pathological grade and tissue classification.The remission rate (RR)was significantly higher in group A(65.3 ％)than in other three groups(RR in group C,group D and group B was 48.1％,44.0％,30.8％;all P＜0.05,respectively).There were significant differences in RR between group C,group D and group B(48.1 ％,44.0％,30.8％ respectively,P＜0.05).The OS of four groups were(510.5 ±20.9)d,(215.7±31.5)d,(326.9 ± 25.7)d,(298.7±21.2)d respectively.The OS was significantly longer in group A than in other groups(P ＜ 0.05).Conclusions The expression of pCRMP2 and Ⅲ β tubulin protein in NSCLC can be used to judge prognosis of lung cancer and to predict chemotherapy drug resistance.

Analysis of adverse events following immunization in Tianjin (2011-2013) evaluated by active surveillance and passive surveillance / 天津医药

Objective To analyze the occurrence features of the active surveillance of adverse events following immu?nization (AEFI) in Tianjin, and evaluate the sensitivity of passive surveillance systems over the same period. Methods The layered eight vaccination clinics of four counties were selected as active surveillance points. The data of active surveillance AEFI were collected through telephone investigation in 2011-2013. The data of passive surveillance was collected through the national AEFI information management system. The descriptive methodology and chi square test were used. Results A total of 235 cases were collected through active surveillance in 2011-2013. The AEFI incidence rate was 718.70 per 100 000 doses,no difference in the incidence between three years(χ2=5.07). A total of 4164 cases were collected through passive sur?veillance. The incidence rate was 34.09 per 100 000 doses,the incidence increased year by year(χ2=572.02,P<0.05). In ac?tive surveillance cases, 95.32%was the common vaccine reaction, 3.83%was the rare vaccine reaction, no serious vaccinereaction. In passive surveillance cases, the common vaccine reaction, the rare vaccine reaction were 85.09%and 13.32%. The serious vaccine reaction was found in 44 cases. Conclusion The more slightly common vaccine reaction can be found by active surveillance. The sensitivity of Tianjin passive surveillance system is increasing year by year, which can meet the requirements for job. The classification and diagnostic criteria of the serious vaccine reaction need to be established.

Evaluation of immune effect of Tianjin Binhai New Area of varicella vaccine / 中国免疫学杂志

To observe the incidence of varicella vaccine after breaking through the case of varicella vaccine , immunization strategy ,popularized in the city.Inoculation Population living in the Binhai New Area of full age to 12 years old children in December,has been vaccinated or who have had chickenpox varicella vaccine except .Controls were four districts around the city girls without varicella in children.Methods: Implementation of vaccination for the target population.All vaccinees was observed from 42 days to 2 and a half years later ,the incidence of varicella break cases.In the observation group and the control group was observed in two groups of varicella vaccine protection rate calculation.Results: The gelatin free attenuated varicella vaccine breakthrough in 134 cases,the incidence rate was 0.35%;no gelatin attenuated varicella vaccine protection rate of 80.92%, with domestic and foreign reports consistent.Conclusion:After vaccination from 42 to 2 and a half years ,varicella vaccine can effectively protect children from the onset, while reducing the prevalence of children 's pain and the economic burden of the family.Varicella vaccine is still a breakthrough occurred ,therefore to consider two inoculations ,with further observation of two times after inoculation the body to produce antibody level and epidemiological protection effect.

Second stage surgery for repair of unilateral cleft lip based on the design of nose and mouth contour / 中华医学美学美容杂志

Objective To investigate the approach and clinical efficacy of second repair surgery according to the nose and mouth contour design of unilateral cleft lip.Methods Based on the characteristics of anatomy,mechanics and deformity of the cleft lip,we designed a surgical incision of nasolabial contour lines with reference to the horizontal and central vertical lines and the contour.After careful investigation of classic procedures and clinical practice,unilateral cleft lip was repaired by overall stage Ⅱ surgical procedures.Results Application of this surgical method with satisfactory clinical efficacy had been achieved.Conclusions The second stage repair personalized surgical methods of unilateral cleft lip designed according to the nose and mouth contour can achieve maximal correction of malformation and beautiful appearance.

Evaluation on the hepatitis A vaccine in preventing hepatitis A infection in Tianjin,from 2000 to 2011 / 中华流行病学杂志

Objective Hepatitis A immunization strategies were carried out in 2001 in Tianjin. We wanted to evaluate the effectiveness of the strategies related to hepatitis A control programs and to provide the basis for further modification of the strategies. Methods Descriptive epidemiology study was used to analyze the hepatitis A epidemic situation in 2000-2011 in Tianjin and to evaluate the disease reporting system. Hepatitis A vaccine coverage of target population and serum epidemiological study were carried out in 1999,2005 and 2010 to check on the hepatitis A antibody levels so as to evaluate the immuno-barrier condition in the normal population. Cox-Stuart test was used to analyze the epidemic trend of hepatitis A and other intestinal infectious diseases in Tianjin. Results The incidence rate of hepatitis A decreased from 2.89/100 000 in 2000 to 0.12/100 000 in 2011,and the percentage of hepatitis A in all types of viral hepatitis decreased from 8.02%in 2000 to 0.48% in 2011 in Tianjin. The positive rates of hepatitis A antibody also increased in the residents. Conclusion The hepatitis A vaccination program was successful in the programs on prevention and control of hepatitis A in Tianjin,China.

Evaluation on the hepatitis A vaccine in preventing hepatitis A infection in Tianjin,from 2000 to 2011 / 中华流行病学杂志

Objective Hepatitis A immunization strategies were carried out in 2001 in Tianjin. We wanted to evaluate the effectiveness of the strategies related to hepatitis A control programs and to provide the basis for further modification of the strategies. Methods Descriptive epidemiology study was used to analyze the hepatitis A epidemic situation in 2000-2011 in Tianjin and to evaluate the disease reporting system. Hepatitis A vaccine coverage of target population and serum epidemiological study were carried out in 1999,2005 and 2010 to check on the hepatitis A antibody levels so as to evaluate the immuno-barrier condition in the normal population. Cox-Stuart test was used to analyze the epidemic trend of hepatitis A and other intestinal infectious diseases in Tianjin. Results The incidence rate of hepatitis A decreased from 2.89/100 000 in 2000 to 0.12/100 000 in 2011,and the percentage of hepatitis A in all types of viral hepatitis decreased from 8.02%in 2000 to 0.48% in 2011 in Tianjin. The positive rates of hepatitis A antibody also increased in the residents. Conclusion The hepatitis A vaccination program was successful in the programs on prevention and control of hepatitis A in Tianjin,China.

Evaluation on the hepatitis A vaccine in preventing hepatitis A infection in Tianjin, from 2000 to 2011 / 中华流行病学杂志

<p><b>OBJECTIVE</b>Hepatitis A immunization strategies were carried out in 2001 in Tianjin. We wanted to evaluate the effectiveness of the strategies related to hepatitis A control programs and to provide the basis for further modification of the strategies.</p><p><b>METHODS</b>Descriptive epidemiology study was used to analyze the hepatitis A epidemic situation in 2000-2011 in Tianjin and to evaluate the disease reporting system. Hepatitis A vaccine coverage of target population and serum epidemiological study were carried out in 1999, 2005 and 2010 to check on the hepatitis A antibody levels so as to evaluate the immuno-barrier condition in the normal population. Cox-Stuart test was used to analyze the epidemic trend of hepatitis A and other intestinal infectious diseases in Tianjin.</p><p><b>RESULTS</b>The incidence rate of hepatitis A decreased from 2.89/100 000 in 2000 to 0.12/100 000 in 2011, and the percentage of hepatitis A in all types of viral hepatitis decreased from 8.02% in 2000 to 0.48% in 2011 in Tianjin. The positive rates of hepatitis A antibody also increased in the residents.</p><p><b>CONCLUSION</b>The hepatitis A vaccination program was successful in the programs on prevention and control of hepatitis A in Tianjin, China.</p>

A sero-epidemiological study on pertussis among the community-based populations in Tianjin during 2010-2012 / 中华流行病学杂志

Objective To study the sero-epidemiology of pertussis immunity and related factors among the community-based populations in Tianjin.Methods Antibodies to pertussis toxin immunoglobulin and IgG (PT-IgG) levels among the community-based populations were examined,using ELISA over the three consecutive years.Subjects that were older than 4 years of age,with concentration of anti-PT-IgG higher than 40 IU/ml were recognized as having recent Bordetella (B.) pertussis infection.Results Of the 1 825 study subjects,the average positive rate of anti-PT-IgG was 10.96％.The highest rate appeared as 24.37％-13.61％ among the 0-3 year-olds (P＜0.001).The positive rate was 8.84％,and the estimated incidence of recent infection became 10 852 per 100 000 among those age 4-83 year-olds.Peak of the estimated incidence rate of recent infection was 18 986 per 100 000 in the age group of 51-83 year-olds (P=0.001),but increasing linearly along with the increase of age (r=0.976,P＜0.001).There were statistically significant differences seen in the antibody positive rate of 3-year period of study (P=0.001),and appeared linear correlation with the reported annual incidence (r =0.992,P＜ 0.001).There were statistically significant differences in the antibody positive rates of the 3 monitoring areas (P=0.034),also showing a linear correlation with the reported annual incidence (r=0.996,P＜0.001).Conclusion Results from this study indicated that B.pertussis infection had been common,particularly in adults living in the communities in Tianjin,calling for the current pertussis immunization strategy to be improved in order to control the pertussis reemerge in China.

Epidemiological investigation of host and focus of natural infection on hemorrhagic fever with renal syndrome in migration areas of the Yangtze River Three-Gorge Reservoir Chongqing region / 第三军医大学学报

Objective　To ascertain the natural infection rate of hemorrhagic fever with renal syndrome virus (HFRSV) among its hosts and the type of the natural foci for providing some baseline data for the immigrant health and epidemic prevention of the Three-Gorge region. Methods　Epidemiological survey on the field was performed including epidemiological data collection, ecology of rodents and pathogen detection. HFRS virus antigen of hosts were detected by the direct immunofluorescent assay (DIFA) technique and determination of HFRSV-RNA by ISH were carried out from HFRSV-Ag-positive animals. Results　HFRSV-Ag-positive animals were found in 5 migration areas ie Baitao Town of Fuling Section, Wansheng Village of Fengjie County and Dachang Town of Wushan County. The positive hosts were as follows, Rattus Norvegicus, Apodemus agrarius, Anourusurex squamipes, Mus musculus and Rattus flavipectus. The positive rate of HFRSV in the mice of 5 migration areas were 19.4%, 17.0%, 14.0%, 13.7%, and 8.5% respectively. The results showed that the lung tissues of some hosts in all five migration areas were HFRSV-RNA-positive. Baitao Town and Peishi Town were attributed to mixture type epidemic areas while. Kangle Town, Wansheng Village and Dachang Town were domestic rats type epidemic areas. Conclusion　This study shows that the five migration areas are natural epidemic foci of HFRS. It is predicted that maximum risk of HFRS breakout or epidemic may take place after the completion of the San Xia Reservoir(the Three-Gorges Reservoir), which results from rodent moving toward higher land. Therefore, deratization and preventive measures for rat are important in migration areas.

Vinorelbine plus oxaliplatin in the treatment of advanced non-small-cell lung cancer / 中国癌症杂志

0.05). The main toxicities were well tolerated and consisted of leukopenia,nausea/vomiting and peripheral neuritis. Grades Ⅲ-Ⅳ nausea/vomiting,renal toxicity and cardiac insufficiency occurred more frequently in the control group,peripheral neuritis occurred more frequently in the study group (P