Cuticular property affects the insecticidal synergy of major constituents in thyme oil against houseflies, Musca domestica.

Plant essential oils are intricate blends comprising predominantly of monoterpenes and some sesquiterpenes. These oils display diverse bioactivities against targeted organisms, often arising from complex interactions among their constituents, which may demonstrate synergistic or antagonistic effects. Despite their wide use as botanical insecticides, the mechanisms behind these interactions and their effects on bioactivity are poorly understood. This study investigated the synergistic interaction of thymol and p-cymene, two major constituents of Thymus vulgaris essential oil, on the larvae and adults of the housefly, Musca domestica. The results showed that p-cymene synergized the insecticidal activity of thymol in adult houseflies, but not in larvae. GC-MS analyses and bioassays indicated the increased cuticular penetration of thymol by p-cymene was the mechanism of synergy, which was observed only in the adults. Two potential routes were proposed: the expansion of the wetting area, or the disruption of cuticular integrity through dissolving the wax layer. The sequential application and large-volume treatment bioassay results suggested that the former was the more likely mechanism. Also, the hydrophobicity of the cuticle seemed critical for this stage-specific synergy. Wax-devoid adults failed to show synergistic toxicity, whereas artificially wax-coated larvae gained a synergistic effect. Overall, the findings provide insights into the synergistic mechanism of insecticidal activity of plant essential oils and suggest potential applications in developing effective strategies using penetration-enhancing synergists.

Real-Time Context-Aware Recommendation System for Tourism.

Recently, the tourism trend has been shifting towards the Tourism 2.0 paradigm due to increased travel experiences and the increase in acquiring and sharing information through the Internet. The Tourism 2.0 paradigm requires developing intelligent tourism service tools for positive effects such as time savings and marketing utilization. Existing tourism service tools recommend tourist destinations based on the relationship between tourists and tourist destinations or tourism patterns, so it is difficult to make recommendations in situations where information is insufficient or changes in real time. In this paper, we propose a real-time recommendation system for tourism (R2Tour) that responds to changing situations in real time, such as external factors and distance information, and recommends customized tourist destinations according to the type of tourist. R2Tour trains a machine learning model with situational information such as temperature and precipitation and tourist profiles such as gender and age to recommend the top five nearby tourist destinations. To verify the recommendation performance of R2Tour, six machine learning models, including K-NN and SVM, and information on tourist attractions in Jeju Island were used. As a result of the experiment, R2Tour was verified with accuracy of 77.3%, micro-F1 0.773, and macro-F1 0.415. Since R2Tour trains tourism patterns based on situational information, it is possible to recommend new tourist destinations and respond to changing situations in real time. In the future, R2Tour can be installed in vehicles to recommend nearby tourist destinations or expanded to tasks in the tourism industry, such as a smart target advertising system.

Synergistic modes of interaction between the plant essential oils and the respiratory blocker chlorfenapyr.

Plant essential oils are widely acknowledged for their insecticidal activities and synergistic interaction with conventional insecticides, but their insecticidal modes of action and the mechanism of synergy remain less understood. In this study, electrophysiological screenings on the larval central nervous system (CNS) of the common fruit fly, Drosophila melanogaster, and the housefly, Musca domestica, were conducted to identify the neurophysiological effects of the oils and their major constituents. Several oils changed the firing rate of the central motor neurons, and four oils were selected to determine their major active compounds. Eugenol and thymol (87.2% and 31.1% in clove bud and thyme oils, respectively) were inhibitory to the nerve firing rates of the CNS, and exhibited synergistic toxicity to the housefly when blended with a respiratory blocking pyrrole insecticide, chlorfenapyr. On the other hand, trans-cinnamaldehyde and terpinen-4-ol (74.6% and 52.0% in cinnamon and teatree oils) seemed excitatory to the nerves, and displayed antagonistic interaction to chlorfenapyr in their insecticidal activity. Chlorfenapyr led to ATP depletion in the insects, and the inhibitory compounds accelerated the process. On the other hand, nerve-excitatory compounds seemed to nullify the depletion. This was further confirmed with the two CNS-excitatory synthetic insecticides, permethrin and chlorpyrifos, that they exhibited antagonistic toxicity when mixed with chlorfenapyr. Meanwhile, the synergy between the inhibitory compounds and chlorfenapyr was diminished when ATP was artificially injected, indicating that the bioenergetic effects of neuroinhibitors are responsible for their synergistic interactions.

Spatio-temporal patterns in pedestrian crashes and their determining factors: Application of a space-time cube analysis model.

The number of vehicle accidents involving pedestrians in Korea has decreased gradually since the Pedestrian Safety and Convenience Enhancement Act was enacted in 2012, but the number of serious pedestrian-related crashes per capital remains near the top of a list of such rates for member countries of the Organization of Economic Cooperation and Development. Previous studies of pedestrian safety have been conducted based on various built environments. However, few have analyzed spatio-temporal changes and influential factors over more than 10 years, despite dramatic changes in the built environment during such time spans. Here, we examine big data on pedestrian-related crashes in Seoul from 2009 to 2018 using a space-time cube methodology and binary logistic regression analysis. The results show that the trend in pedestrians killed or severely injured is decreasing with pedestrian environment enhancement projects and pedestrian safety measures in Seoul. Also, the analysis reveals a need to pay more attention to pedestrian safety in areas with a large older population. Pedestrian safety measures should be reinforced in areas of concentrated wholesale and retail businesses. This study also indicates that illegal parking poses a threat to pedestrian safety. Lastly, this study confirms some positive impacts of redeveloped or newly developed areas and pedestrian environment enhancement projects on pedestrian safety.

Does elderly safety matter? Associations between built environments and pedestrian crashes in Seoul, Korea.

Korea's elderly population is growing rapidly, as is attention to elderly pedestrian safety. Despite a consensus that the elderly are vulnerable to pedestrian safety issues, our understanding of the determinants of elderly pedestrian crashes is limited. This study explores which attributes of the built environment affect the risk of pedestrian accidents among the elderly, particularly with respect to injury severity, in Seoul, Korea. We compare the impacts of various determinants on pedestrian crashes to specify how the associations between various built environments and pedestrian accidents differ by pedestrian age. We also examine how the associations vary by neighborhood economic attributes. Our findings provide policy implications for identifying various attributes of the built environment that increase the risk of elderly pedestrian crashes and improving the safety of elderly pedestrian by neighborhood economic status.

Efficacy of Seven Commercial Household Aerosol Insecticides and Formulation-Dependent Toxicity Against Asian Tiger Mosquito (Diptera: Culicidae).

For the indoor and outdoor pest controls, various types of insecticide formulations are available including aerosols, sprays, electric vaporizers, mosquito coils, and traps. In the present study, the insecticidal activity of aerosols, the most commonly used formulation of household insecticides for mosquito control, against Aedes albopictus (Skuse) was assessed using seven commercial products and some attributes which can affect the efficacy of aerosol were investigated as well. The products had difference in their chemical composition of active ingredients, propellant/liquid phase ratios, solvent types, and nozzle orifice sizes, and these characteristics seem to affect the overall insecticidal activity. In general, solvent type dominantly determined the insecticidal activity, where four products in oil-based solvent system showed greater mortality (97.5% in average) than water-based aerosols (38.3% in average) against the mosquitoes located at the far side of the test chamber. The contribution of solvent type and nozzle orifice size were further examined with the sample aerosols, and the orifice size were determined more influential to the spray distance. Regardless of solvent types, the sample products attached to a bigger actuator (0.96 mm in diameter) showed greater knock-down activity (>98%) than the smaller ones (0.48 mm, 62.5% in average) to the back panel in the chamber. On the other hand, solvent system significantly affected the residual activity, as the oil-based and water-based aerosols showed 2.3- and 4.8-fold decrease in KT50 values, respectively, between 1 and 10 min after the spray.

The Impact of Preoperative α-Adrenergic Antagonists on Ureteral Access Sheath Insertion Force and the Upper Limit of Force Required to Avoid Ureteral Mucosal Injury: A Randomized Controlled Study.

PURPOSE: Excessive bulking force during primary access of the ureteral access sheath may induce ureteral injury. We investigated the efficacy of preoperative α-blockade to reduce ureteral access sheath insertion force and determine the upper limit required to avoid ureteral injury. MATERIALS AND METHODS: In this randomized controlled trial 135 patients from a single institution who had ureteropelvic junction or renal pelvis stones and were scheduled to undergo retrograde intrarenal surgery were prospectively enrolled from December 2015 to January 2017. Of the patients 41 and 42 were randomly assigned to the control and experimental groups, respectively. The experimental group received α-blockade preoperatively. The 21 patients who were pre-stented were assessed separately. We developed a homemade device to measure maximal ureteral access sheath insertion force. RESULTS: Our ureteral access sheath insertion force measurement device showed excellent reproducibility. Higher insertion velocity resulted in greater maximal sheath insertion force. Maximal insertion force in the α-blockade group was significantly lower than in the control group at the ureterovesical junction (p = 0.008) and the proximal ureter (p = 0.036). Maximal insertion force in the α-blockade group was comparable to that in pre-stented patients. Female patients and patients 70 years old or older showed a lower maximal ureteral access sheath insertion force than their counterparts. The rate of grade 2 or greater ureteral injury was lower in the α-blockade group than in controls (p = 0.038). No injury occurred in any case in which ureteral access sheath insertion force did not exceed 600 G. CONCLUSIONS: Preoperative α-blockade and slow sheath placement may reduce maximal ureteral access sheath insertion force. If the force exceeds 600 G, a smaller diameter sheath may be an alternative. Alternatively the procedure can be terminated and followed later by pre-stented retrograde intrarenal surgery.

Effect of hydrothermal processing on ginseng extract.

BACKGROUND: Panax ginseng Meyer is cultivated because of its medicinal effects on the immune system, blood pressure, and cancer. Major ginsenosides in fresh ginseng are converted to minor ginsenosides by structural changes such as hydrolysis and dehydration. The transformed ginsenosides are generally more bioavailable and bioactive than the primary ginsenosides. Therefore, in this study, hydrothermal processing was applied to ginseng preparation to increase the yields of the transformed ginsenosides, such as 20(S)-Rg3, Rk1, and Rg5, and enhance antioxidant activities in an effective way. METHODS: Ginseng extract was hydrothermally processed using batch reactors at 100-160°C with differing reaction times. Quantitative analysis of the ginsenoside yields was performed using HPLC, and the antioxidant activity was qualitatively analyzed by evaluating 2,2'-azino-bis radical cation scavenging, 2,2-diphenyl-1-picrylhydrazyl radical scavenging, and phenolic antioxidants. Red ginseng and sun ginseng were prepared by conventional steaming as the control group. RESULTS: Unlike steaming, the hydrothermal process was performed under homogeneous conditions. Chemical reaction, heat transfer, and mass transfer are generally more efficient in homogeneous reactions. Therefore, maximum yields for the hydrothermal process were 2.5-25 times higher than those for steaming, and the antioxidant activities showed 1.6-4-fold increases for the hydrothermal process. Moreover, the reaction time was decreased from 3 h to 15-35 min using hydrothermal processing. CONCLUSION: Therefore, hydrothermal processing offers significant improvements over the conventional steaming process. In particular, at temperatures over 140°C, high yields of the transformed ginsenosides and increased antioxidant activities were obtained in tens of minutes.

Hydrolysis kinetics of tulip tree xylan in hot compressed water.

Lignocellulosic biomass, a promising renewable resource, can be converted into numerous valuable chemicals post enzymatic saccharification. However, the efficacy of enzymatic saccharification of lignocellulosic biomass is low; therefore, pretreatment is necessary to improve the efficiency. Here, a kinetic analysis was carried out on xylan hydrolysis, after hot compressed water pretreatment of the lignocellulosic biomass conducted at 180-220°C for 5-30min, and on subsequent xylooligosaccharide hydrolysis. The weight ratio of fast-reacting xylan to slow-reacting xylan was 5.25 in tulip tree. Our kinetic results were applied to three different reaction systems to improve the pretreatment efficiency. We found that semi-continuous reactor is promising. Lower reaction temperatures and shorter space times in semi-continuous reactor are recommended for improving xylan conversion and xylooligosaccharide yield. In the theoretical calculation, 95% of xylooligosaccharide yield and xylan conversion were achieved simultaneously with high selectivity (desired product/undesired product) of 100 or more.

General Strategy for Broadband Coherent Perfect Absorption and Multi-wavelength All-optical Switching Based on Epsilon-Near-Zero Multilayer Films.

We propose a general, easy-to-implement scheme for broadband coherent perfect absorption (CPA) using epsilon-near-zero (ENZ) multilayer films. Specifically, we employ indium tin oxide (ITO) as a tunable ENZ material, and theoretically investigate CPA in the near-infrared region. We first derive general CPA conditions using the scattering matrix and the admittance matching methods. Then, by combining these two methods, we extract analytic expressions for all relevant parameters for CPA. Based on this theoretical framework, we proceed to study ENZ CPA in a single layer ITO film and apply it to all-optical switching. Finally, using an ITO multilayer of different ENZ wavelengths, we implement broadband ENZ CPA structures and investigate multi-wavelength all-optical switching in the technologically important telecommunication window. In our design, the admittance matching diagram was employed to graphically extract not only the structural parameters (the film thicknesses and incident angles), but also the input beam parameters (the irradiance ratio and phase difference between two input beams). We find that the multi-wavelength all-optical switching in our broadband ENZ CPA system can be fully controlled by the phase difference between two input beams. The simple but general design principles and analyses in this work can be widely used in various thin-film devices.

Broadband Epsilon-Near-Zero Perfect Absorption in the Near-Infrared.

Perfect absorption (PA) of incident light is important for both fundamental light-matter interaction studies and practical device applications. PA studies so far have mainly used resonant nanostructures that require delicate structural patterning. Here, we realize tunable and broadband PA in the near-infrared region using relatively simple thin film coatings. We adjust the growth condition of an ITO film and control its epsilon-near-zero (ENZ) wavelength. We show that this results in highly tunable PA in the telecommunication window. Then, using an ITO multilayer of different ENZ wavelengths, we demonstrate broadband PA that covers a wide range of near-infrared wavelengths. The use of ENZ coatings makes PA adjustable during the film growth and does not require any structural patterning afterward. It also facilitates the chip-scale integration of perfect absorbers with other device components. Broadband PA relaxes the single wavelength condition in previous PA studies, and thus it is suitable for many practical device applications, including sensors, photodetectors, and energy harvesting devices.

Preparation and characterization of nano-sized hydroxyapatite/alginate/chitosan composite scaffolds for bone tissue engineering.

The aim of this study was to develop chitosan composite scaffolds with high strength and controlled pore structures by homogenously dispersed nano-sized hydroxyapatite (nano-HAp) powders. In the fabrication of composite scaffolds, nano-HAp powders distributed in an alginate (AG) solution with a pH higher than 10 were mixed with a chitosan (CS) solution and then freeze dried. While the HAp content increased up to 70 wt.%, the compressive strength and the elastic modulus of the composite scaffolds significantly increased from 0.27 MPa and 4.42 MPa to 0.68 MPa and 13.35 MPa, respectively. Higher content of the HAp also helped develop more differentiation and mineralization of the MC3T3-E1 cells on the composite scaffolds. The uniform pore structure and the excellent mechanical properties of the HAp/CS composite scaffolds likely resulted from the use of the AG solution at pH 10 as a dispersant for the nano-HAp powders.

Vibronic structures and dynamics of the predissociating dimethyl sulfide and its isotopomers (CH3SCH3, CD3SCD3, CH3SCD3) at the conical intersection.

Conical intersection seam comprised of crossing surfaces of two lowest excited states of dimethyl sulfide (DMS) has been directly accessed by the one-photon excitation from the ground equilibrium state. Since the S-C bond rupture takes place promptly, the molecular structure on the excited state effectively belongs to C(S) symmetry. Namely, excited states of 1(1)B1 and 1(1)A2 in C(2)V become 1(1)A'' and 2(1)A'' states in C(S), respectively, and the optical transition from the ground equilibrium state to the dissociating molecule at the conical intersection seam is symmetry-allowed to facilitate the nonadiabatic transition on the 2(1)A'' state, leading eventually to the CH3S + CH3 products. The dynamic study of DMS, in this sense, gives the great opportunity to unravel the vibronic structure of the conical intersection seam by the conventional one-photon excitation method. In this work, utilizing the photofragment excitation (PHOFEX) spectroscopic method, the vibronic structures of DMS and its isotope analogs (CD3SCD3, CH3SCD3) at the conical intersection seam have been revealed, providing accurate lifetimes and detailed dynamics associated with individual vibronic transitions. The lifetime of the excited DMS is estimated to be ~100 fs, indicating that the dissociation is complete within one single oscillation in the conical intersection region. It is also found that the symmetric CSC stretching mode is strongly coupled to the reaction coordinate, as manifested by our experimental finding that the fragmentation yield of the S-CD3 bond is enhanced compared to that of the S-CH3 bond in the CH3SCD3 dissociation reaction only when the CSC symmetric stretching vibrational mode is excited at the conical intersection region. This work demonstrates that the better understanding of the excited state could make the bond-selective chemistry into reality.

Conical intersection seam and bound resonances embedded in continuum observed in the photodissociation of thioanisole-d3.

Herein, the multi-dimensional nature of the conical intersection seam has been experimentally revealed in the photodissociation reaction of thioanisole-d3 (C6H5SCD3) excited on S1, giving C6H5S·(à or X̃]) +·CD3 products. The translational energy distribution of the nascent·CD3 fragment, reflecting the relative yields of the C6H5S·(Ã) and C6H5S·(X̃) products, was measured at each S1 vibronic band using the velocity map ion imaging technique. Direct access of the reactant flux to the conical intersection seam leads to the increase of the nonadiabatic transition probability resulting in sharp resonances in the X̃/ÃC6H5S·product branching ratio at several distinct S1 vibronic bands. The nature of the S1 vibronic bands associated with such dynamic resonances was clarified by the mass-analyzed threshold ionization spectroscopy. The bound state embedded in continuum generated by the conical intersection is observed as a distinct dynamic resonance, revealing the nature of the nuclear motion responsible for the nonadiabatic coupling of two potential energy surfaces at the conical intersection. The multi-dimensional facets of the conical intersection seam in terms of its detailed structure and dynamic role are discussed with the aid of theoretical calculations.

Evaluation of hot compressed water pretreatment and enzymatic saccharification of tulip tree sawdust using severity factors.

Tulip tree sawdust was pretreated using hot compressed water with different pretreatment severities (LogR0, 3.05-5.01) by varying reaction temperatures (180-220°C) and residence time (1-30 min). It is found that the chemical composition and physicochemical properties of the pretreated products can be characterized and correlated with severity. Removal of most of the xylan and other hemicellulosic sugars from the raw material was observed at a severity of 4.5. Thus, the residual solids were recovered with increased cellulose and lignin contents. Nearly complete glucan conversion was achieved after 48 h of hydrolysis with 10 FPU/g of wet residual solid obtained above a severity of 4.8. The characteristics of the pretreated solids according to the pretreatment severity were strongly related with the glucose yield. The removal of structural barriers to the enzyme attack was the dominant factor affecting enzyme accessibility to the substrate.

Impact of bleaching on subcritical water- and Formosolv-pretreated tulip tree to enhance enzyme accessibility.

A novel method was developed for fractionating cellulose microfibrils from forest residue (tulip tree sawdust) to enhance cellulose digestibility, particularly at minimum enzyme loadings. This method involved three main stages: selective hemicellulose solubilization by subcritical water (SCW) pretreatment, delignification of the SCW-pretreated solids using the Formosolv process, and deformylation/bleaching of the cellulose pulp with alkaline hydrogen peroxide solution. This process produced nearly 98% white cellulose microfibrils with 23-fold higher conversion to glucose as compared to the raw substrate after 72 h of enzymatic hydrolysis. This study showed that cellulose swelling had the greatest effect on the enzymatic hydrolysis efficiency of delignified pulp obtained by the Formosolv process.

Resonance spectrum of a three-dimensional photonic quantum ring laser with an equilateral triangle microcavity.

We have fabricated three-dimensional (3D) photonic quantum ring lasers with an equilateral triangle microcavity. Their spectra were well explained by combining the off-normal resonance and hexagonally bounced in-plane whispering-gallery-mode condition. The angular distribution of the emission modes and their discrete wavelengths were shown to be in excellent agreement with a 3D Rayleigh Fabry-Perot model. We confirmed that the allowed modes in the equilateral triangle microcavity decrease by decreasing the length of equilateral triangle side, L, and the spectral mode spacing linearly increases with the mode index m and is inversely proportional to L2.