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J Forensic Sci ; 65(1): 283-287, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31436852

RESUMO

Species identification of necrophagous insects found on a dead body is an essential key in applying medicolegal entomology to the estimation of postmortem interval (PMI). Due to limited morphological identification of insect evidence, several studies have identified species using molecular information such as DNA markers. While considerable cytochrome c oxidase subunit I (COI) gene sequence data of necrophagous fly species have been collected and annotated, those of necrophagous beetle species have not. Since necrophagous beetles such as Dermestes species have a larval period longer than that of flies, beetles are useful in even the late decomposition phase in estimating minimum PMI. To obtain the full-length COI gene sequences of six Dermestes species collected from South Korea, we designed primers for polymerase chain reaction amplification and sequencing. The obtained full COI nucleotide sequences were used for performing phylogenic analysis and comparison with previously reported sequences. The results demonstrated that the COI gene sequences could be used to identify forensically important Dermestes species in South Korea.


Assuntos
Besouros/genética , Complexo IV da Cadeia de Transporte de Elétrons/genética , Análise de Sequência de DNA , Animais , Primers do DNA , Entomologia Forense , Larva , Filogenia , Reação em Cadeia da Polimerase , República da Coreia , Especificidade da Espécie
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