Handheld metal detector confirmation of radiopaque foreign bodies in the esophagus.

OBJECTIVE: To examine the utility of handheld metal detectors in confirming the position of radiopaque foreign bodies in the esophagus before delayed endoscopic removal. DESIGN: Prospective study of patients evaluated between June 1, 1997, and August 31, 1999. SETTING: Tertiary pediatric referral center. PATIENTS: Twenty-six of 139 children presenting consecutively for evaluation of esophageal foreign bodies met eligibility criteria and completed the study protocol. Inclusion in the study was contingent on a delay of at least 6 hours from the time of diagnosis to the time of endoscopic removal. All patients underwent both radiographic evaluation and handheld metal detector scanning of the chest and abdomen on presentation and immediately before endoscopic removal. RESULTS: All patients evaluated during the study period had coins lodged within the esophagus. Handheld metal detector scanning accurately confirmed this position before endoscopic removal in all cases. CONCLUSION: Our data suggest that handheld metal detectors may obviate the need for repeated radiographs in patients whose foreign bodies cannot be removed at presentation.

Characterization of clustered MHC-linked olfactory receptor genes in human and mouse.

Olfactory receptor (OR) loci frequently cluster and are present on most human chromosomes. They are members of the seven transmembrane receptor (7-TM) superfamily and, as such, are part of one of the largest mammalian multigene families, with an estimated copy number of up to 1000 ORs per haploid genome. As their name implies, ORs are known to be involved in the perception of odors and possibly also in other, nonolfaction-related, functions. Here, we report the characterization of ORs that are part of the MHC-linked OR clusters in human and mouse (partial sequence only). These clusters are of particular interest because of their possible involvement in olfaction-driven mate selection. In total, we describe 50 novel OR loci (36 human, 14 murine), making the human MHC-linked cluster the largest sequenced OR cluster in any organism so far. Comparative and phylogenetic analyses confirm the cluster to be MHC-linked but divergent in both species and allow the identification of at least one ortholog that will be useful for future regulatory and functional studies. Quantitative feature analysis shows clear evidence of duplications of blocks of OR genes and reveals the entire cluster to have a genomic environment that is very different from its neighboring regions. Based on in silico transcript analysis, we also present evidence of extensive long-distance splicing in the 5'-untranslated regions and, for the first time, of alternative splicing within the single coding exon of ORs. Taken together with our previous finding that ORs are also polymorphic, the presented data indicate that the expression, function, and evolution of these interesting genes might be more complex than previously thought.

The interrelationship of subglottic air pressure, fundamental frequency, and vocal intensity during speech.

In this study we have simultaneously measured subglottic air pressure, airflow, and vocal intensity during speech in nine healthy subjects. Subglottic air pressure was measured directly by puncture of the cricothyroid membrane. The results show that the interaction between these aerodynamic properties is much more complex that previously believed. Certain trends were seen in most individuals, such as an increase in vocal intensity with increased subglottic air pressure. However, there was considerable variability in the overall aerodynamic properties between subjects and at different frequency and intensity ranges. At certain frequencies several subjects were able to generate significantly louder voices without a comparable increase in subglottic air pressure. We hypothesize that these increases in vocal efficiency are due to changes in vocal fold vibration properties. The relationship between fundamental frequency and subglottic pressure was also noted to vary depending on vocal intensity. Possible mechanisms for these behaviors are discussed.