Matching N supply for yield maximization in salt-affected wheat agri-food systems: On-farm participatory assessment and validation.

Driven by the UN-SDGs of achieving food security and agricultural sustainability, it remains more challenging in degraded ecosystems to simultaneously improve the crop performance without creating unintended favour for excessive fertilization and associated environmental consequences. We assessed the N-use pattern of 105 wheat-growers in sodicity affected Ghaghar Basin of Haryana, India, and then experimented upon to optimize and identify indicators of efficient N use in contrasting wheat cultivars for sustainable production. The survey results revealed that majority of farmers (88%) have increased their reliance on N nutrition (∼18 % extra N), and even extended their duration of N scheduling (12-15 days) for better plant adaptation and yield insurance in sodicity stressed wheat; albeit to a greater extent in moderately sodic soils applying 192 kg N ha-1 in 62 days. The participatory trials validated the farmers' perception of using more than the recommended N in sodic lands. This could realize the transformative improvements in plant physiological [higher photosynthetic rate (Pn; 5 %) and transpiration rate (E; 9 %)] and yield [more tillers (ET; 3 %), grains spike-1 (GS; 6 %) and healthier grains (TGW; 3 %)] traits culminating in ∼20 % higher yield at 200 kg N ha-1 (N200). However, further incremental N application had no apparent yield advantage or monetary benefits. At N200, every additional kilogram of N captured by the crop beyond the recommended N improved grain yields by 36.1 kg ha-1 in KRL 210 and 33.7 kg ha-1 in HD 2967. Further, the varietal differences for N requirements, with 173 kg ha-1 in KRL 210 and 188 kg ha-1 in HD 2967, warrants the need of applying balanced fertilizer dose and advocate revision of existing N recommendations to cope up the sodicity induced agricultural vulnerability. Principal Component Analysis (PCA) and correlation matrix showed N uptake efficiency (NUpE) and total N uptake (TNUP) as the highly weighted variables illustrating strong positive association with grain yield, and potentially deciding the fate of proper N utilization in sodicity stressed wheat. Key insights suggested that combining participatory research with farmers' knowledge and local perspective could be decisive in better integration of technologies, and serving to adapt the real-time soil sodicity stress and sustaining wheat yields with economized farm profits.

Computational analysis and functional characterisation of Tor putitora toll-like receptor 4 with the elucidation of its binding sites for microbial mimicking ligands.

In the current study, full-length Toll-like receptor 4 (TLR4) cDNA was cloned and characterised in Tor putitora, an important fish inhibiting Himalayan rivers. The complete coding sequence of TpTLR4 is 2457 bp with nine key structural domains, including six leucine-rich repeats (LRRs). The phylogenetic tree revealed that TpTLR4 showed the closest relationship with TLR4 of Cyprinus carpio (96%), Labeo rohita (91%) and Megalobrama amblycephala (88%), all belonging to the Cyprinidae family. CELLO2GO tool revealed that TpTLR4 protein is highly localised in the plasma (67.7%), and the protein has a strong association with myeloid differentiation primary response 88 (MYD88) followed by Tumor necrosis factor receptor-associated factor (TRAF) family. In the toll-interleukin-1 receptor (TIR) domain of TpTLR4, the proline is replaced by the alanine amino acid, thus may give plasticity to the receptor to recognise both bacterial and viral ligands. Molecular docking has revealed that TpTLR4 showed the strongest affinity towards poly (I:C) with the binding energy of -6.1 kcal/mol and five hydrogen bonds among all ligands. Based on our molecular docking results, it can be presumed that TpTLR4 can sense bacterial, fungal and viral molecular patterns with binding sites mainly present in the TpTLR4 LRR9 motif, which spans between 515 and 602 amino acids. Tor putiora TLR4 transcript was ubiquitously expressed in all the tested fish tissues. Although, transcript level was found to be highest in blood and spleen followed by the kidney. The TpTLR4 transcripts showed peak expression in spleen and kidney at 12 h post-injection (hpi) (p < 0.05) of poly (I:C). The constitutive expression of TpTLR4 in various tissues, up-regulation in different tissues and strong binding affinities with poly (I:C) indicate that TpTLR4 may play an essential role in sensing pathogen-associated molecular patterns (PAMPs), particularly of viral origin.

Ecological and human health risk assessment of trace element pollution in sediments and five important commercial fishes of the Oman Sea.

The distribution of trace elements in sediments and five commercially important fish species of the Oman Sea were assessed using an atomic absorption spectrometer to determine ecological and human health risk assessment. The ranges of trace elements concentration in sediments were: Cr: 5.42-68.94, Zn: 5.22-18.11, Cu: 1.44-4.87, As: 18.07-79.96, Cd: 0.10-1.27, Ni: 43.0-65.34 and Pb: 8.92-30.53 mg/kg dry weight. Cr, Zn, Cu, Cd and Pb were below the ERL (effects range low) and TEL (threshold effect level) values at all the sampling stations with rarely biological effects. Whereas, As and Ni were higher than ERL and TEL with occasionally biological effects. The patterns of trace elements bioaccumulation in the tissues of all species were: liver> skin> gill> muscle. A significant positive correlation suggests that trace elements accumulation in fish is determined by trace elements in sediments. Estimated Daily Intake (EDI) was significantly lower than the tolerable daily intake (TDI) value. Target Hazard quotient (THQ), total target hazard quotient (TTHQ) and target carcinogenic risk (TR) values indicated no hazard risk from fish consumption. Hence, the consumption of concerned species to people in the Oman Sea is safe.