Co-pyrolysis of chicken manure with tree bark for reduced biochar toxicity and enhanced plant growth in Arabidopsis thaliana.

Co-pyrolysis of chicken manure with tree bark was investigated to mitigate salinity and potentially toxic element (PTE) concentrations of chicken manure-derived biochar. The effect of tree bark addition (0, 25, 50, 75 and 100 wt%) on the biochar composition, surface functional groups, PTEs and polycyclic aromatic hydrocarbons (PAH) concentration in the biochar was evaluated. Biochar-induced toxicity was assessed using an in-house plant growth assay with Arabidopsis thaliana. This study shows that PTE concentrations can be controlled through co-pyrolysis. More than 50 wt% of tree bark must be added to chicken manure to reduce the concentrations below the European Biochar Certificate-AGRO (EBC-AGRO) threshold. However, the amount of PAH does not show a trend with tree bark addition. Furthermore, co-pyrolysis biochar promotes plant growth at different application concentrations, whereas pure application of 100 wt% tree bark or chicken manure biochar results in decreased growth compared to the reference. In addition, increased plant stress was observed for 100 wt% chicken manure biochar. These data indicate that co-pyrolysis of chicken manure and tree bark produces EBC-AGRO-compliant biochar with the potential to stimulate plant growth. Further studies need to assess the effect of these biochars in long-term growth experiments.

The screening of various biochars for Cd2+ removal at relevant soil pH.

Fourteen biochars from seven biomass sources were investigated on their long-term Cd2+ removal. The experiments consisted of a ten-day batch Cd2+ adsorption in a pH-buffered solution (pH = 6) to minimise pH effects. Insect frass, spent peat and chicken manure-derived biochars are promising Cd2+ adsorbents. Pyrolysis temperature was crucial for optimising Cd2+ removal by insect frass and spent peat-derived biochars. For these biochars, a pyrolysis temperature of 450 °C was optimal. In contrast, the Cd2+ removal by chicken manure biochars was independent of pyrolysis temperature. The Cd2+ removal by insect-frass and spent peat-derived biochars was associated with chemisorption on surface functionalities, while using chicken manure biochars was more associated with Cd2+ precipitation. The kinetics of Cd2+ removal over the course of ten days showed that insect frass biochar (450 °C) showed a gradual increase from 36 to 75 % Cd2+ removal, while chicken manure and spent peat-derived biochar (450 °C) already showed a higher Cd2+ removal (72 - 89 %) after day 1. This evidences that a long-term Cd2+ removal effect can be expected for some biochars. This should certainly be taken into consideration in future soil-based experiments.

Enhanced cesium removal from real matrices by nickel-hexacyanoferrate modified activated carbons.

After nuclear disasters, radioactive cesium partitions to soils and surface water, where it decays slowly. Hexacyanoferrates (HCFs) have excellent cesium removal properties but their structure is typically powdery. Many carrier materials, such as biomass or magnetic particles, have been used to provide a suitable substrate for HCFs that can be used in filters. This research uses the sorption properties of activated carbon (AC) to incorporate Ni-HCF, resulting in good structural properties of the hybrid material. These HCF-modified ACs show drastically improved sorption properties towards Cs after one, two and three HCF impregnation cycles. The activated carbon from brewer's spent grain with one modification cycle removes more than 80% of 1 mg L-1 Cs in a sea water solution and more than 98% of 1 mg L-1 Cs from surface water at a low AC dosage (0.5 g L-1). Iron and nickel leaching is studied and found to be dependent on the type of modified AC used and the leaching solution. Iron leaching can be problematic in surface and seawater, whereas nickel leaching is especially pronounced in seawater.

Characterisation of adsorbents prepared by pyrolysis of sludge and sludge/disposal filter cake mix.

Copper and zinc removal from water (pH = 5.0) using adsorbents produced from slow and fast pyrolysis of industrial sludge and industrial sludge mixed with a disposal filter cake (FC), post treated with HCl, is investigated in comparison with a commercial adsorbent F400. The results show that a pseudo-second order kinetics model is followed. The Langmuir-Freundlich isotherm model is found to fit the data best. The capacity for heavy metal removal of studied adsorbents is generally better than that of commercial F400. The dominant heavy metal removal mechanism is cation exchange. Higher heavy metal removal capacity is associated with fast pyrolysis adsorbents and sludge/FC derived adsorbents, due to enhanced cation exchange. Improvement of Zn(2+) removal via 1 N HCl post-treatment is only effective when exchangeable cations of the adsorbent are substituted with H(+) ions, which boost the cation exchange capacity. Increase of temperature also enhances metal removal capacity. Fast pyrolysis sludge-based adsorbents can be reused after several adsorption-desorption cycles.

Economic assessment of flash co-pyrolysis of short rotation coppice and biopolymer waste streams.

The disposal problem associated with phytoextraction of farmland polluted with heavy metals by means of willow requires a biomass conversion technique which meets both ecological and economical needs. Combustion and gasification of willow require special and costly flue gas treatment to avoid re-emission of the metals in the atmosphere, whereas flash pyrolysis mainly results in the production of (almost) metal free bio-oil with a relatively high water content. Flash co-pyrolysis of biomass and waste of biopolymers synergistically improves the characteristics of the pyrolysis process: e.g. reduction of the water content of the bio-oil, more bio-oil and less char production and an increase of the HHV of the oil. This research paper investigates the economic consequences of the synergistic effects of flash co-pyrolysis of 1:1 w/w ratio blends of willow and different biopolymer waste streams via cost-benefit analysis and Monte Carlo simulations taking into account uncertainties. In all cases economic opportunities of flash co-pyrolysis of biomass with biopolymer waste are improved compared to flash pyrolysis of pure willow. Of all the biopolymers under investigation, polyhydroxybutyrate (PHB) is the most promising, followed by Eastar, Biopearls, potato starch, polylactic acid (PLA), corn starch and Solanyl in order of decreasing profits. Taking into account uncertainties, flash co-pyrolysis is expected to be cheaper than composting biopolymer waste streams, except for corn starch. If uncertainty increases, composting also becomes more interesting than flash co-pyrolysis for waste of Solanyl. If the investment expenditure is 15% higher in practice than estimated, the preference for flash co-pyrolysis compared to composting biopolymer waste becomes less clear. Only when the system of green current certificates is dismissed, composting clearly is a much cheaper processing technique for disposing of biopolymer waste.

Hydrodechlorination of dichlorobiphenyls over Ni-Mo/Al2O3 catalysts prepared by spray-drying method.

The hydrodechlorination (HDCl) process of 2,3-, 2,4- and 2,5-dichlorobiphenyls was studied over a sulphided Ni-Mo/Al(2)O(3) catalyst in a stirred autoclave at a hydrogen pressure of 3 MPa. The catalysts were prepared by spray-drying. They were characterized by N(2) adsorption, thermogravimetry and scanning electron microscopy with X-ray microanalysis. The reaction temperature of the catalytic HDCl process was varied in the range of 230-290 degrees C. Polychlorinated biphenyls (PCBs) free transformer oil was used as reaction medium. The HDCl degree of dichlorobiphenyl isomers was in the range of 82-93%. The efficiency in the chlorine removal was found to be related to the position of the substituted chlorine atom and decreased as follows 2,4-dichlorobiphenyl approximately 2,5-dichlorobiphenyl>2,3-dichlorobiphenyl. For comparison, the HDCl process of 2,3-dichlorobiphenyl (2,3-PCB) without catalyst was also studied. The chlorine removal was 85% for the catalytic HDCl of 2,3-PCB whereas non-catalytic process led only to 16% of dechlorination in the same operating conditions, i.e. at 290 degrees C after 120 min. Monodichlorobiphenyls were not detected in the reaction products. The data for both catalytic and non-catalytic conversion of 2,3-PCB fit to a first-order model. Kinetic constants and the activation energy of the overall HDCl reaction of 2,3-PCB to biphenyl were evaluated. Compared to non-catalytic process, a nearly threefold decrease in the activation energy was observed in the presence of Ni-Mo/Al(2)O(3) catalyst prepared by spray-drying (48 kJ mol(-1) vs. 124 kJ mol(-1)).

Molecular and functional characterization of ovine cardiac valve-derived interstitial cells in primary isolates and cultures.

At present the involvement of cardiac valve interstitial cells (VICs) in growth, repair, and tissue engineering is understudied. Therefore, this study aims at characterizing ovine VICs in order to provide a solid base for tissue engineering of heart valves. Ovine ICs of the four heart valves were isolated by the explant outgrowth method and expanded in vitro up to passage 5. Vimentin and collagen I gene expression from freshly isolated or cultured ICs was measured by reverse transcriptase-polymerase chain reaction. Immunocytochemical stainings of vimentin, alpha-smooth muscle actin (ASMA), smooth muscle myosin, and procollagen I were performed on aortic VICs. In addition, migration and extracellular matrix deposition were studied in vitro in aortic VICs. ICs show stable vimentin and collagen I expression in culture. Expression is approximately doubled in cultured ICs compared with fresh isolates. More than 95% of ICs in each passage stain for vimentin and procollagen I. Freshly isolated ICs are ASMA and myosin negative, but ICs in culture partially stain for these contractile markers. ICs have stable matrix production for up to five passages, associated with stable migration of the cells. We conclude that ovine valve interstitial cells undergo phenotypic modulation to activated myofibroblasts under culture conditions but retain stable matrix production.

Antimineralization treatments in stentless porcine bioprostheses: an experimental study.

BACKGROUND AND AIM OF THE STUDY: Photo-oxidation treatment of porcine stentless bioprostheses (Photofix) was compared with glutaraldehyde fixation, with either AOA (Freestyle valve) or Tween-80 (Edwards Prima Plus valve). METHODS: Six valves of each type were implanted in juvenile sheep, in the pulmonary position. Valves were explanted after three or six months and examined macroscopically, by X-radiography, and by light and transmission electron microscopy. Calcium content was determined by atomic absorption spectrometry. RESULTS: The cusps of all valves were free of calcification, and had normal histology and function. Calcium contents (median +/- IQR) were 0.63+/-0.45, 0.73+/-1.46 and 0.46+/-1.42 microg/mg for the Photofix, Freestyle and Prima Plus valves, respectively (p = NS). Calcium contents of the aortic wall portions were 0.71+/-1.27 (Photofix), 10.78+/-77.22 (Freestyle) and 28.70+/-66.53 (Prima Plus) (p <0.05 for Photofix versus Freestyle or Prima Plus). CONCLUSION: Photo-oxidation of a porcine stentless valve prevents calcification not only in the cusps, but also in the aortic wall portion.

Calcification characteristics of porcine stented valves in a juvenile sheep model.

BACKGROUND: Different antimineralization treatments of stented porcine bioprostheses were evaluated: ethanol (Epic), alpha-amino-oleic acid (AOA) (Mosaic), and sodium dodecyl sulfate (SDS) (Hancock II). A nontreated, glutaraldehyde-fixed valve (Labcor) served as control. METHODS: For each treatment, six valves were implanted in juvenile sheep in the pulmonary position. Valves were explanted after 3 and 6 months and examined macroscopically, by roentgenogram and light and transmission electron microscopy. Calcium content (microg/mg) was determined by atomic absorption spectrometry. RESULTS: The Labcor valves revealed small calcium deposits in the cusps, although calcium content remained low (median value 0.4+/-0.8 microg/mg). SDS did not prevent cusp calcification as assessed by histology and calcium content measurement, which was higher than in all other valves: 1.9+/-4.6 microg/mg (p < 0.05). Cusp retraction and rupture were occasionally found in the Hancock. The Mosaic and Epic valves showed no cusp calcification and had low calcium contents (0.3+/-2.4 microg/mg and 0.7+/-0.6 microg/mg, respectively). Epic showed less pannus formation, but had hematoma or iron staining in the cusps. CONCLUSIONS: SDS is inefficient as an antimineralization treatment, in contrast to ethanol or AOA. Cusp hematoma after ethanol treatment needs further investigation.

Lack of calcification of tissue engineered heart valves in juvenile sheep.

The presence of viable endothelial cells may support longer durability and the absence of calcification in valve prostheses. This study shows the development of a tissue engineered heart valve, constructed from viable autologous endothelial cells on an acellular matrix and its evaluation in juvenile sheep. In 3-month-old sheep (n = 8) a piece of vein was harvested to culture autologous endothelial cells. A porcine acellular matrix was reendothelialized and implanted in juvenile sheep as pulmonary interposition. The valves were explanted after 1 week, 3 and 6 months. Examination was performed by X-ray, light microscopy, and atomic absorption spectrometry. Reendothelialization mean rate was 10.3 x 10(5) cells/cm(2) with a mean endothelial cell viability of 95.5% (0.98 x 10(5) cells/cm(2)). X-ray examination showed no cusp calcification at 1 week, 3 and 6 months, which was confirmed by light microscopy. Immunostaining for factor VIII demonstrated colonization of viable mature autologous endothelial cells on the heart valve after the seeding process. The atomic absorption spectrometry showed no significant increase of the calcium content after 3 (P value >.1) and 6 months (P value >.1) compared with nonimplanted tissue engineered heart valves. The tissue engineered valve showed no cusp calcification in the juvenile sheep after 3 and 6 months.

A new design of lead amalgam/lead sulphate electrode for potentiometric measurement of sulphate.

A new lead amalgam/lead sulphate electrode has been developed which is easy to handle and can be used in a simple measuring cell. Its electrochemical characteristics have been tested in two different cell systems. The potentials obtained were very stable, and reproducible within +/-0.04 mV. The electrode exhibited a Nernstian response to sulphate and its calculated standard potential (-350.68 +/- 0.13 mV) agreed very closely with that recorded in the literature.

Potentiometric investigation of the stability of silver(I) complexes of some N-methyl substituted 4-H-diethylenetriamines in aqueous solution.

By means of potentiometric pH and pAg measurements, the stability constants and the stoichiometric composition of the silver(I) complexes of some N-methyl-substituted 4-H-diethylenetriamines, in aqueous medium of ionic strength 1.3 and at a temperature of 25.00 degrees , have been determined. In addition to mononuclear and polynuclear complexes, together with their protonated forms, some hydroxo complexes are formed. The values of the stability constants are discussed in terms of possible structures.