Giant ventral hernia simultaneously containing the spleen, a portion of the pancreas and the left hepatic lobe: A case report.

BACKGROUND: Ventral hernia, also known as incisional hernia, is a common complication of previous surgery. The contents of ventral hernia may include omentum, preperitoneal fat, small intestine or colon. However, ventral hernia with protrusion of more than two parenchymal organs simultaneously is extremely rare, and its repair is very complex and difficult. Surgeons should make a comprehensive assessment based on their own experience and the individual characteristics of the hernia. In addition, psychological therapy should be emphasized in the whole treatment process. CASE SUMMARY: We report a rare case of asymptomatic giant ventral hernia for 15 years in a 21-year-old female. The patient underwent umbilical hernia repair at the age of 1 year. Approximately 5 years later, ventral hernia recurred and repair with Mesh was performed, but the operation failed due to postoperative infection, and a huge mass appeared in the left abdominal wall. The mass increased gradually with the development and maturity of the body. Computerized tomography scan demonstrated that the patient's total spleen, part of the pancreas and left lobe of the liver were simultaneously herniated through the abdominal incisional hernia. As the patient was unable to endure the inconvenience of life and the potential risk of spleen or liver rupture, she underwent a ventral hernia repair with Mesh at our hospital. The operation was successful and the patient had a good recovery. During a 3-mo follow-up, the patient remained asymptomatic and the appearance of the surgical incision was greatly improved. CONCLUSION: Ventral hernia is a common complication of abdominal surgery. Ventral hernia with protrusion of more than two parenchymal organs simultaneously is extremely rare. Surgeons should pay attention to the psychological treatment while restoring the abdominal physiological function in ventral hernia patients.

Intermittent abdominal pain accompanied by defecation difficulties caused by Chilaiditi syndrome: A case report.

We report a case of intermittent lower abdominal pain and distension accompanied by defecation difficulties for 3 years due to Chilaiditi syndrome in a 59-year-old male. Before admission to our hospital, the patient had undergone gastroscopy, which showed gastritis and duodenitis, and colonoscopy, which showed cecum deformation and cicatricial changes of the mucous membrane in the colon hepatic flexure. A computed tomography (CT) scan of the abdomen at our hospital confirmed right hepatic atrophy and interposition of the colon. Moreover, CT simulation endoscopy identified cystic dilatation in the colon hepatic flexure with the widest diameter of 8.2 cm. The patient was diagnosed with Chilaiditi syndrome. As the patient was unable to endure his defecation difficulties, he underwent a laparoscope-assisted right hemicolectomy. The patient had a good recovery. During the follow-up period of 9 mo, the patient remained symptom-free.

Comparison of Efficacy of Laparoscopic and Open Splenectomy Combined With Selective and Nonselective Pericardial Devascularization in Portal Hypertension Patients.

BACKGROUND: Recent advances in laparoscopic techniques have demonstrated that laparoscopy is superior for managing portal hypertension. This study aimed to compare the efficacy of laparoscopic splenectomy plus selective pericardial devascularization (LSSD) and open splenectomy and nonselective pericardial devascularization (OSD) in patients with portal hypertension. METHODS: A total of 127 patients with portal hypertension underwent either LSSD or OSD at Jingzhou Central Hospital from January 2014 to December 2016. A total of, 55 patients received LSSD, including 29 male and 26 female individuals (age, 56.0±11.6 y); 72 patients received OSD, including 45 male and 27 female individuals (age, 53.4±10.9 y). The parameters of operation and outcomes were measured and compared. RESULTS: Intraoperative blood loss, postoperative complications, and hospital stay were significantly lower in LSSD group but operation time was significantly lower in OSD group. Postoperative Child-Pugh score was significantly lower in LSSD group than in OSD group. CONCLUSIONS: LSSD is a safe and effective treatment for liver cirrhosis patients with portal hypertension.

The expression of PLK-1 in cervical carcinoma: a possible target for enhancing chemosensitivity.

BACKGROUND: Polo-like kinase-1 (PLK-1) is reported to be upregulated in a variety of human tumors and is implicated in cell proliferation and survival. However, its importance in cervical carcinoma has not yet been fully elucidated. METHODS: We examined PLK-1 expression in cervical carcinoma tissues using immunohistochemical staining. Furthermore, we blocked PLK-1 expression in HeLa cells using specific siRNA and detected the cell cycle, cell proliferation and chemosensitivity using western blotting, MTT and flow cytometry. RESULTS: We provide evidence that expression of PLK-1 exists in human cervical carcinoma tissues and establish an association with tumor size. Furthermore, we show that PLK-1 knockdown by transfection of siRNA induces accumulation of HeLa cells in the G2/M cell cycle phase and enhances cisplatin-induced apoptosis. CONCLUSION: Our results indicate that PLK-1 production in HeLa cells might be critical in determining whether cells survive or undergo apoptosis. Therefore, targeting PLK-1 might be a promising strategy for enhancing sensitivity to chemotherapeutic reagents in cervical carcinoma.

[Expressions of cyclins A, B1, D3, and E in asynchronous and synchronous MOLT-4 cells of G1 phase].

BACKGROUND & OBJECTIVE: We have testified expression imbalance of Cyclins A, B1, D3, and E in MOLT-4 cells, which were synchronized by "double thymidine blocks", with flow cytometry (FCM). However, comparisons of Cyclins expressions between synchronized cells and asynchronized cells haven't been performed at that time because of technique limitations. This study was to compare expressions of Cyclins A, B1, D3, and E in G(1) phase of asynchronized and synchronized cells by newly established "postsorting Western blot", and to conform the unreasonableness of using double thymidine blocks to synchronize cells to analyze normal cell cycles. METHODS: MOLT-4 cells were synchronized at G(1) phase by double thymidine blocks, asynchronous cells of G(1) phase were sorted by FCM. Western blot and double parameters analysis of DNA/Cyclins were performed to detect Cyclins A, B1, D3, and E expressions in asynchronous and synchronous MOLT-4 cells of G(1) phase. RESULTS: There were almost no expressions of Cyclins A, B1 in asynchronous MOLT-4 cells of G(1) phase, and obvious expressions in synchronized cells of G(1) phase. Expressions of Cyclins D3, E in synchronous MOLT-4 cells of G(1) phase were higher than those in asynchronous cells of G(1) phase. The FCM results were accordant with Western blot results. CONCLUSIONS: The expressions of Cyclins in synchronized cells obtained through "double thymidine blocks" can't represent their expressions in normal cells. Thus, synchronous cells produced by "double thymidine blocks" are not ideal experimental models for analyzing normal cell cycles.