[Establishment of a Patient-Derived T-Cell Acute Lymphoblastic Leukemia Xenograft Model in Novel Immunodeficient NCG Mice].

OBJECTIVE: The leukemia cells from patients with T-cell acute lymphoblastic leukemia (T-ALL) were inoculated into NCG mice to establish a stable human T-ALL leukemia animal model. METHODS: Leukemia cells from bone marrow of newly diagnosed T-ALL patients were isolated, and the leukemia cells were inoculated into NCG mice via tail vein. The proportion of hCD45 positive cells in peripheral blood of the mice was detected regularly by flow cytometry, and the infiltration of leukemia cells in bone marrow, liver, spleen and other organs of the mice was detected by pathology and immunohistochemistry. After the first generation mice model was successfully established, the spleen cells from the first generation mice were inoculated into the second generation mice, and after the second generation mice model was successfully established, the spleen cells from the second generation mice were further inoculated into the third generation mice, and the growth of leukemia cells in peripheral blood of the mice in each group was monitored by regular flow cytometry to evaluate the stability of this T-ALL leukemia animal model. RESULTS: On the 10th day after inoculation, hCD45+ leukemia cells could be successfully detected in the peripheral blood of the first generation mice, and the proportion of these cells was gradually increased. On average, the mice appeared listless 6 or 7 weeks after inoculation, and a large number of T lymphocyte leukemia cells were found in the peripheral blood and bone marrow smear of the mice. The spleen of the mice was obviously enlarged, and immunohistochemical examination showed that hCD3+ leukemia cells infiltrated into bone marrow, liver and spleen extensively. The second and third generation mice could stably develop leukemia, and the average survival time was 4-5 weeks. CONCLUSION: Inoculating leukemia cells from bone marrow of patients with T-ALL into NCG mice via tail vein can successfully construct a patient-derived tumor xenografts (PDTX) model.

Controlling internal nitrogen and phosphorus loading using Ca-poor soil capping in shallow eutrophic lakes: Long-term effects and mechanisms.

Clean soil is a potential capping material for controlling internal nutrient loading and helping the recovery of macrophytes in eutrophic lakes, but the long-term effects and underlying mechanisms of clean soil capping under in-situ conditions remain poorly understood. In this study, a three-year field capping enclosure experiment combining intact sediment core incubation, in-situ porewater sampling, isotherm adsorption experiments and analysis of sediment nitrogen (N) and phosphorus (P) fractions was conducted to assess the long-term performance of clean soil capping on internal loading in Lake Taihu. Our results indicate that clean soil has excellent P adsorption and retention capacity as an ecologically safe capping material and can effectively mitigate NH4+-N and SRP (soluble reactive P) fluxes at the sediment-water interface (SWI) and porewater SRP concentration for one year after capping. The mean NH4+-N and SRP fluxes of capping sediment were 34.86 mg m-2 h-1 and -1.58 mg m-2 h-1, compared 82.99 mg m-2 h-1 and 6.29 mg m-2 h-1 for control sediment. Clean soil controls internal NH4+-N release through cation (mainly Al3+) exchange mechanisms, while for SRP, clean soil can not only react with SRP due to its high Al and Fe content, but also stimulate the migration of active Ca2+ to the capping layer, thus precipitating as Ca-bound P (Ca-P). Clean soil capping also contributed to the restoration of macrophytes during the growing season. However, the effect of controlling internal nutrient loading only lasted for one year under in-situ conditions, after which the sediment properties returned to pre-capping conditions. Our results highlight that clean Ca-poor soil is a promising capping material and further research is needed to extend the longevity of this geoengineering technology.

Soil moisture determines nitrous oxide emission and uptake.

Soils are major sources and sinks of nitrous oxide (N2O). The main pathway of N2O emission is performed through soil denitrification; however, the uptake phenomenon in denitrification is overlooked, leading to an underestimation of N2O production. Soil moisture strongly influences denitrification rates, but exact quantifications coupled with nosZ, nirK, and nirS gene analysis remain inadequately unaccounted for. In this study, a 15N-N2O pool dilution (15N2OPD) method was used to measure N2O production rates under different soil moisture levels. Therefore, 20%, 40%, 60%, 80% and 100% soil water holding capacity (WHC) were used. The results revealed that N2O uptake rates increased proportionally with soil moisture content and peaked at 80% WHC with 4.17 ± 2.74 µg N kg-1 soil h-1. The N2O production and net emission rates similarly peaked at 80% WHC, reading at 32.50 ± 4.86 and 27.63 ± 3.09 µg N kg-1 soil h-1 during the incubation period (18 days). Soil moisture content increased the gene copy number of the nosZ, NH4+ content, and denitrification potential in soil. N2O uptake at WHC 80-100% was significantly greater than that at WHC 20-60%. It was attributed to a decrease in O2 and the high NO3- concentration inhibition (> 50 mg N kg-1 of soil NO3--N content). Principal components analysis (PCA) indicated that the number of nosZ genes was the major driver of N2O uptake, especially nosZ clade II. Thus, the results of this study deepen our understanding of the mechanisms underpinning N2O sources and sinks in soils and provide a useful gene-based indicator to estimate N2O uptake.

Pathways of soil N2O uptake, consumption, and its driving factors: a review.

Nitrous oxide (N2O) is an important greenhouse gas that plays a significant role in atmospheric photochemical reactions and contributes to stratospheric ozone depletion. Soils are the main sources of N2O emissions. In recent years, it has been demonstrated that soil is not only a source but also a sink of N2O uptake and consumption. N2O emissions at the soil surface are the result of gross N2O production, uptake, and consumption, which are co-occurring processes. Soil N2O uptake and consumption are complex biological processes, and their mechanisms are still worth an in-depth systematic study. This paper aimed to systematically address the current research progress on soil N2O uptake and consumption. Based on a bibliometric perspective, this study has highlighted the pathways of soil N2O uptake and consumption and their driving factors and measurement techniques. This systematic review of N2O uptake and consumption will help to further understand N transformations and soil N2O emissions.

Effect of dredging and capping with clean soil on the mitigation of algae-induced black blooms in Lake Taihu, China: A simulation study.

Sediment is an important source of matter that causes blackening and odor formation in a water body. The restoration of polluted sediment can suppress algae-induced black blooms to a certain degree. In this study, we compared the control effects of sediment dredging and capping with clean soil on algae-induced black blooms in Lake Taihu using indoor simulation experiments. In addition, we explored the driving effect of temperature on algae-induced black blooms using the method of gradual warming (18, 23, and 28 °C) during the experiment. No blackening of the water body was observed in the simulation stages I (18 °C) and II (23 °C), and the blackening and odor formation occurred within 3 d when the temperature increased to 28 °C in stage III, implying that high temperature was an important driving factor for algae-induced black blooms. Dredging and capping inhibited the blackening and odor formation to some extent, and the colorimetric values in the water columns were lower in the treatment groups than in the control group. At the end of the experiment, the colorimetric values of dredging and capping treatments were 56.5% and 96.7% of the colorimetric value of the control group, respectively. The control effect of dredging on the blackening elements, i.e., Fe2+ and S2- and the main odor forming compounds, i.e., dimethyl disulfide (DMDS) and dimethyl trisulfide (DMTS) was observed in stage II (11-20 d) and stage III (21-27 d), respectively, and the inhibition ability of dredging to suppress algal-induced black blooms was superior than that of capping with clean soil.

Understanding the Heavy Metal Pollution Pattern in Sediments of a Typical Small- and Medium-Sized Reservoir in China.

Heavy metal pollution in sediments is a common environmental issue in small- and medium-sized reservoirs not only in China but also worldwide; however, few interpretations of the pollution pattern exist. Based on the analyses of accumulation characteristics, ecological risks, and source apportionments of eight heavy metals (As, Cd, Cr, Cu, Hg, Pb, Ni, and Zn) in sediments, we derived a paradigm to describe the pollution pattern of heavy metals in sediments of a typical small- and medium-sized Tongjiqiao Reservoir. The results showed high levels of Cd, Hg, and As pollutants in the surface and upper sediment layers of the pre-dam area. Additionally, As, Cd, Hg, and Pb pollutants peaked in the middle layers of the inflow area, indicating a high ecological risk in these areas. The positive matrix factorization results implied that industrial, agricultural, and transportation activities were the main sources of heavy metals. The heavy metal pollution pattern exhibited three distinct stages: low contamination, rapid pollution, and pollution control. This pattern explains the heavy metal pollution process in the sediments and will provide scientific guidance for realizing the green and sustainable operation and development of the reservoir.

Alterations of amino acid metabolism and intestinal microbiota in Chinese mitten crab (Eriocheir sinensis) fed on formulated diet and iced trash fish.

Formulated diet (FD) and iced trash fish (ITF) are common diets during E. sinensis farming. However, whether FD can completely replace ITF during long-term E. sinensis farming is still unclear. Thus this study was conducted to compare the differences in amino acid metabolism and intestinal microbiota of the E. sinensis fed on different diets. The crabs were randomly divided into three groups fed on FD, ITF and mixed diet (MD, FD: ITF = 1:1), respectively. The results showed that there were no significant differences in amino acid composition among FD, MD and ITF groups. The activities of AST and ALT, and the mRNA levels of amino acid metabolism-related genes were significantly up-regulated in FD or/and MD groups compared with ITF group. The diversity of intestinal bacterial community was similar between the FD and ITF groups, but the relative abundance of dominant taxa showed marked differences between the two groups. At the phylum level, the relative abundance of Firmicutes was significantly higher, but the relative abundance of Proteobacteria was significantly lower in the FD group than that in ITF group. Meanwhile, at genus level, the relative abundance of Candidatus_Hepatoplasma in FD was higher than that in ITF group. Data related to functional prediction demonstrated that the significantly differenced pathways between the two groups were observed in metabolism (Pyrimidine metabolism, Glycolysis/Gluconeogenesis and Citrate cycle) and environmental information processing (transporters). The overall results indicated that replacement of ITF by FD did not affect amino acid composition, but altered amino acid metabolism and the relative abundance of intestinal microbiota. Our data provided a valuable reference for FD application replacing ITF during long-term E. sinensis farming.

Parallel subgenome structure and divergent expression evolution of allo-tetraploid common carp and goldfish.

How two subgenomes in allo-tetraploids adapt to coexistence and coordinate through structure and expression evolution requires extensive studies. In the present study, we report an improved genome assembly of allo-tetraploid common carp, an updated genome annotation of allo-tetraploid goldfish and the chromosome-scale assemblies of a progenitor-like diploid Puntius tetrazona and an outgroup diploid Paracanthobrama guichenoti. Parallel subgenome structure evolution in the allo-tetraploids was featured with equivalent chromosome components, higher protein identities, similar transposon divergence and contents, homoeologous exchanges, better synteny level, strong sequence compensation and symmetric purifying selection. Furthermore, we observed subgenome expression divergence processes in the allo-tetraploids, including inter-/intrasubgenome trans-splicing events, expression dominance, decreased expression levels, dosage compensation, stronger expression correlation, dynamic functionalization and balancing of differential expression. The potential disorders introduced by different progenitors in the allo-tetraploids were hypothesized to be alleviated by increasing structural homogeneity and performing versatile expression processes. Resequencing three common carp strains revealed two major ecotypes and uncovered candidate genes relevant to growth and survival rate.

Identification, expression and enzyme activity of the group III sPLA2 s in Cyprinus carpio L.

Five group III secreted phospholipase (pla2g3s) homologous genes located on different linkage groups were identified from common carp (Cyprinus carpio), which we named Ccpla2g3a1, Ccpla2g3a2, Ccpla2g3b, Ccpla2g3c1 and Ccpla2g3c2. The five genes encode 530, 525, 461, 752 and 753 amino acids, respectively. Sequence analysis showed that the Ccpla2g3as contain seven exons and the others contain four exons. Synteny analysis of fish pla2g3s indicated that pla2g3a and pla2g3b were from the same ancestor gene, and Ccpla2g3a1, Ccpla2g3a2, Ccpla2g3c1 and Ccpla2g3c2 were from the specific genome duplication of common carp. Due to the significant variation of the pla2g3bs from common carp and zebrafish (Danio rerio), they formed a separate group in the phylogenetic tree. The tissue distributions of Ccpla2g3s coincided with their expression profiles during the embryo stages. The expression levels of Ccpla2g3as and Ccpla2g3cs were low at the embryo stages, and they were abundant in the liver and brain, respectively, whereas the expression of Ccpla2g3b was high at 0.5 h after fertilization and in the ovary. We obtained three soluble recombinant proteins of the bee venom-like PLA2 (BVLP) from Ccpla2g3 and evaluated their PLA2 enzyme properties. The optimum pHs of MBP-a1-BVLP, MBP-b-BVLP and MBP-c1-BVLP were 7.5, 7.0 and 8.0, respectively, and specific activities were 7.68 ± 0.66, 4.155 ± 0.158 and 1.93 ± 0.05 U µmol-1 , respectively. The Kd for Ca2+ of MBP-b-BVLP was the lowest (2.6 µM), whereas the values for both MBP-a1-BVLP and MBP-c1-BVLP were about 15 µM. The Km values of three proteins ranged from 31.9 to 41.91 µM.

Identification, expression and pro-inflammatory effect of interleukin-17 N in common carp (Cyprinus carpio L.).

Two interleukin (IL)-17 N genes (CcIL-17Na and b) present on different linkage groups were identified in the common carp (Cyprinus carpio) genome and confirmed by polymerase chain reaction (PCR) and real time (RT)-PCR in this experiment. Synteny analysis revealed that IL-17 N is transcribed by the complement sequence of TOP3B's intron 2. It is flanked by SDF2L and PPM1F in all fish studied to date, except fugu (Takifugu rubripes). The open reading frames of the two CcIL-17Ns are 411 base pairs long and encode 136 amino acids. The amino acid identity/similarity between CcIL-17Na and b is 91.2%/97.1%. The CcIL-17Ns share identity (46.8-90.4%) with their orthologs from other teleosts. Identities/similarities to other members of the IL-17 family in common carp were low at 21.4-30.2%/31.4-51.4%. In the phylogenetic tree, IL-17Ns from spotted gar (Lepisosteus oculatus, the ancestor of teleosts) and coelacanth (Latimeria chalumnae, the ancestor of tetrapods) were grouped within the same branch with a high bootstrap value of 97%, which indicates that IL-17 N is an ancient and conserved gene. Quantitative RT-PCR results showed that CcIL-17Ns were most highly expressed in the brain of healthy individuals. The expression in brain was significantly induced at 6 h post Aeromonas hydrophila infection; at 1 day post infection, expression in liver, muscle, skin, spleen, and head kidney was up-regulated. In addition, the upregulated expression of proinflammatory cytokines IL-1ß, IFN-γ, IL-6, chemokine CCL20, NF - κ B and TRAF6 in kidney tissue by ccIL-17 N recombinant protein also indicate that IL-17 N can promote inflammation through NF-κB pathway and induce the expression of chemokines and inflammatory factors.

Nitrogen budget at sediment-water interface altered by sediment dredging and settling particles: Benefits and drawbacks in managing eutrophication.

Internal nitrogen (N) loading of lakes is commonly controlled by sediment dredging, although its comprehensive effect on internal N loading remains unclear. Herein, we examined the long-term effects of sediment dredging on internal N loading from a new perspective on the N budget at the sediment-water interface (SWI) through a simulation of field dredging performed by incubating intact sediment cores from a shallow eutrophic lake (Lake Taihu). We further evaluated the role of settling particles (SP) in the recovery of N cycle processes after dredging and its potential impact on the N budget. Our results demonstrated that dredging could help reduce organic matter and total N in sediments; improve the redox environment of the SWI; slow down N mineralization, N fixation, denitrification, and anaerobic ammonia oxidation (anammox); and alter the N budget at the SWI and the contribution of various N cycle processes. However, the input of SP enriched in fresh organic matter and N could accelerate the recovery of N cycle processes at the SWI, reducing the variation in the N budget and the contribution of each N cycle process caused by dredging. Dredging significantly reduced the N flux at the SWI, which was evident from the reduction of inorganic N release flux and N removal through denitrification and anammox. Therefore, sediment dredging has its advantages and disadvantages in managing internal N loading in lakes. To maintain a long-term control on the release of internal N through sediment dredging, measures should be taken based on the in-lake and watershed to inhibit the inflow and settlement of particulate matter.

Relationship Between the Fatty Acid Profiles and Gut Bacterial Communities of the Chinese Mitten Crab (Eriocheir sinensis) From Ecologically Different Habitats.

The gut microbiota plays an important role in a variety of physiological functions such as intestinal digestion, metabolic homeostasis, immune response, and responses to disease treatment. Whether there is a relationship between gut microbial communities and fatty acid (FA) profiles of Chinese mitten crab is unclear. Hence, we analyzed the relationship between FA profiles and the gut bacterial communities of six Chinese mitten crab (Eriocheir sinensis) populations from different lakes. The crabs were sampled from six different lakes in Jiangsu Province, China. The FA profiles of these crab populations were compared and clustered, and then used to determine the relationship between geographic location and FA composition. We also characterized the gut microbial communities of these crabs using 16S rRNA high-throughput gene sequencing. The FA profiles varied significantly (P < 0.05) between crabs from different geographical locations. A similar trend was also observed in the gut microbial communities, which also varied significantly based on their geographical origin (P < 0.05). Furthermore, alpha diversity, cluster analysis, and matching bacterial community structures with specific locations revealed patterns that significantly linked FA profiles to the gut microbiota. Further analysis of FA profiles and gut microbial community generated patterns that linked the two parameters. Hence, it was observed that the gut microbial community seems to contribute significantly to the FA composition of the Chinese mitten crab. However, further studies need to be conducted to investigate the interactions between gut microbial communities and the biochemical composition of the Chinese mitten crab, which will ultimately unravel the complexity of microbial ecosystems for potential applications in aquaculture and species conservation.

Molecular insights into information processing and developmental and immune regulation of Eriocheir sinensis megalopa under hyposaline stress.

Eriocheir sinensis is an important euryhaline catadromous crustacean of the Yangtze River and an important commercial species for breeding in China. However, wild E. sinensis have suffered serious damage attributed to overfishing, climate change, etc. The Ministry of Agriculture of China issued a notice banning the commercial fishing of wild E. sinensis. E. sinensis megalopa migrates upriver into fresh water for growth and fattening, which creates optimal conditions to experimentally explore its hyposaline osmoregulation mechanism. We performed comparative transcriptome analyses of E. sinensis megalopae under hyposaline stress. The results suggest that KEGG pathways and genes related to genetic information processing, developmental regulation, immune and anti-stress responses were differentially expressed. The present study reveals the most significantly enriched pathways and functional gene groups, and explores the hyposaline osmoregulation mode of E. sinensis megalopae. This study lays a theoretical foundation for further studies on the osmoregulation and developmental mechanisms of E. sinensis.

Genome Wide Analysis for Growth at Two Growth Stages in A New Fast-Growing Common Carp Strain (Cyprinus carpio L.).

In order to identify candidate genes or loci associated with growth performance of the newly established common carp strain, Xinlong, we conducted a genome-wide association analysis using 2b-RAD technology on 123 individuals. We constructed two sets of libraries associated with growth-related parameters (weight, length, width and depth) measured at two different grow-out stages. Among the 413,059 SNPs identified using SOAP SNP calling, 147,131 were tested for GWAS after quality filtering. Finally, 39 overlapping SNPs, assigned to four genomic locations, were associated with growth traits in two stages. These loci were assigned to functional classes related to immune response, response to stress, neurogenesis, cholesterol metabolism and development, and proliferation and differentiation of cells. By overlapping results of Plink and EMMAX analyses, we identified three genes: TOX, PLK2 and CD163 (both methods P < 0.05). Our study results could be used for marker-assisted selection to further improve the growth of the Xinlong strain, and illustrate that largely different sets of genes drive the growth of carp in the early and late grow-out stages.

Hydropower reservoirs on the upper Mekong River modify nutrient bioavailability downstream.

Hydropower development is the key strategy in many developing countries for energy supply, climate-change mitigation and economic development. However, it is commonly assumed that river dams retain nutrients and therefore reduce downstream primary productivity and fishery catches, compromising food security and causing trans-boundary disputes. Contrary to expectation, here we found that a cascade of reservoirs along the upper Mekong River increased downstream bioavailability of nitrogen and phosphorus. The dams caused phytoplankton density to increase with hydraulic residence time and stratification of the stagnant reservoirs caused hypoxia at depth. This allowed the release of bioavailable phosphorus from the sediment and an increase in dissolved inorganic nitrogen as well as a shift in nitrogen species from nitrate to ammonium, which were transported downstream by the discharge of water from the base of the dam. Our findings provide a new perspective on the environmental impacts of river dams on nutrient cycling and ecosystem functioning, with potential implications for sustainable development of hydropower worldwide.

Molecular insights into the sex-differential regulation of signal transduction in the cerebral ganglion and metabolism in the hepatopancreas of Eriocheir sinensis during reproduction.

The Chinese mitten crab (Eriocheir sinensis), an economically valuable crustacean that is popular for its flavor, exhibits catadromous spawning migration. Overfishing and environmental pollution have inflicted serious damage on wild E. sinensis populations, and the Chinese government has banned the commercial fishing of this species in the Yangtze River. Studies have examined the sexual dimorphism in the body size and morphology of crabs, but there are few reports on the molecular regulatory mechanisms that occur during the reproduction of E. sinensis. In this study, we performed the first comparative transcriptome analyses of the cerebral ganglion and hepatopancreas of E. sinensis during reproduction. The results indicate that E. sinensis has significant sexual dimorphism in signal transduction, metabolism, substance transportation, and cellular protection. This study aims to provide information that can be used as a basis for further research on the molecular mechanisms that underlie sexual dimorphism in E. sinensis during reproduction. Furthermore, the results can be used to support the development of the E. sinensis breeding industry and the restoration of wild E. sinensis.

Water-level alterations modified nitrogen cycling across sediment-water interface in the Three Gorges Reservoir.

Water-level regime alteration-associated redox fluctuation plays a primary role in governing exchange and transformation of nitrogen (N) in water-level fluctuation zones (WLFZs), while few understanding of how hydrological regimes under reservoir operation affected N cycling across the sediment-water interface (SWI), giving rise to uncertainties in reservoir N nutrient management. Batch microcosm simulation experiments with intact sediment cores from WLFZs of the Three Gorges Reservoir (TGR) were conducted for 24 days to identify holistic flooding-drying process mechanism on N-cycling patterns. Our results showed a distinct transition of N-cycling mode across the SWI, shifting from biological denitrogen loss dominated in initial period of flooding to enhance endogenous N retention. A dramatic source-sink switch of nitrous oxide (N2O) occurred in the first 1.5 days during the flooding period. However, combined accelerating migration of NH4+-N from sediment to overlying water, and subsequently enhanced transformation of NH4+-N to NO3--N formed from flooding to drying rotation, thereby increasing N loading to overlying water. The reason for this investigation could be attributed to intensive N loss through coupled nitrification and denitrification in oxic-anoxic microenvironments after flooding. With oxygen replenishment from atmosphere during drying phase, persistent ammonification of organic N in sediments provided sufficient source of NH4+-N for the formation of NO3--N fraction in a more oxic overlying water. Therefore, water-level regime alteration by reservoir operation was capable of weakening N removal from water body and lengthening internal N turnover time across redox-variable SWI. These findings elucidate new understanding of holistic hydrological regime mechanisms on N cycling across SWI and provide insight to biogenic N nutrient management for improving the green credentials of hydroelectric reservoir.

Influence of chlorothalonil and carbendazim fungicides on the transformation processes of urea nitrogen and related microbial populations in soil.

To improve crop yielding, a large amount of fungicides is continuously applied during the agricultural management, while the effects of fungicides residues on microbial processing of N in soil need further study. In the present study, two broad spectrum fungicides, chlorothalonil and carbendazim, were applied at the rates of 5, 10, and 50 mg of active ingredient (A.I.) per kg of dry soil combined with urea with 200 mg of N per kg of dry soil under laboratory conditions. The results showed that chlorothalonil obviously retarded the hydrolysis of urea, whereas carbendazim accelerated it in 4 days after the treatments (P < 0.05). Chlorothalonil reduced denitrification, nitrification, and N2O production (P < 0.05), but not for carbendazim. Further analysis on N-associated microbial communities showed chlorothalonil reduced nitrosomonas populations at the rates of 10 and 50 mg of A.I. per kg and autotrophic nitrifying bacterial populations at three application rates (P < 0.05), but Carbendazim decreased nitrosomonas populations only at the rate of 50 mg of A.I. per kg and also autotrophic nitrifying bacterial populations at three rates and heterotrophic nitrifying bacterial populations at the rates of 10 and 50 mg of A.I. per kg. The reasons for this difference were ascribed to arrest urea hydrolysis and impediment of denitrification and nitrification processes by chlorothalonil. In conclusion, to improve crop yielding, chlorothalonil might be more beneficial to conserve soil N by improving soil N fertility, compared with carbendazim.

Investigating the distribution of the Yangtze finless porpoise in the Yangtze River using environmental DNA.

Determining the distribution of the Yangtze finless porpoise (Neophocaena asiaeorientalis asiaeorientalis, YFP) in the Yangtze River has to date relied on traditional visual and counting methods, but such field surveys are time-consuming and expensive. Analyses using environmental DNA (eDNA) to investigate the presence and range of endangered aquatic species have proven to be more economical and effective detection methods, and are a non-invasive approach to sampling. A challenge of relying on eDNA for YFP monitoring is that the Yangtze River is characterized by high turbidity and a strong current. Here, we used an eDNA-based approach to estimate the presence of YFP at 18 sites in the Yangtze River in August 2017 and at an additional 11 sites in January 2018. At each sampling site, we filtered six 1 L water samples with 5 µm pore size filter paper and quantified the amount of YFP eDNA in each water sample using quantitative real-time polymerase chain reaction (qPCR). In addition, YFP eDNA was successfully detected in locations where we visually observed YFP, as well as in locations where YFP were not observed directly. We found that our eDNA-based method had higher detection rates than traditional field survey methods. Although YFP was visually observed in the Yangtze River in winter, water samples collected during the summer contained significantly higher YFP eDNA than winter water samples. Our results demonstrate the potential effectiveness of eDNA detection methods in determining the distribution of YFP in the Yangtze River.

In situ simulation of thin-layer dredging effects on sediment metal release across the sediment-water interface.

Dredging is widely applied to remediate contaminated sediments in aquatic ecosystems. However, the efficiency of thin-layer dredging for metal pollution control remains uncertain and even controversial. This study conducted an in-situ simulation experiment in Lake Taihu to investigate dredging effects on sediment metal release based on metal fractions, diffusion flux and kinetics parameters of metal resupply, using diffusive gradient in thin films (DGT), multi-microelectrode, and European Community Bureau of Reference (BCR) sequential extraction scheme. Results indicated that the exchange fluxes of metals did not necessarily correspond to total sediment metal concentrations or the contents of different sequentially-extracted metal fractions; there were appreciable decreases in Ni, Cd, Cu and Zn in terms of total sediment metal concentrations and metal fractions, whereas the bioavailability and release fluxes of labile Ni, Cu and Zn (but not Cd) were all notably promoted (by 136, 128 and 149%, respectively) in dredged area compared to those in un-dredged sediments. Further analysis on the kinetics of metal resupply by DGT technique and DGT-induced fluxes in sediments model (DIFS) showed higher concentrations of labile metals, with a larger resupply ability from sediments after dredging. Therefore, thin-layer dredging had the possibility to increase metal release from sediments to the water column. This was attributed to the remobilization of metal sulfides in anoxic deep sediments, as oxidation increased after dredging due to the introduction of oxygenated water, causing subsequent dissolution of sulfide-bound metals. In conclusion, dredging may not mitigate metal contamination, although it can reduce the total pollution load. Our findings indicated dual effects of dredging and provided new insights into the remobilization mechanism of metal release induced by dredging.