[Quantification and fast-screening of malachite green in aquaculture water using paper spray ionization mass spectrometry].

OBJECTIVE: A rapid quantitative technique had been developed for the screening of malachite green(MG) in aquaculture water using paper spray ionization mass spectrometry(PSI-MS). METHODS: To rapid extract MG, chloroform and deep eutectic solvents(DESs) were used as extraction agent and co-solvent respectively. malachite green D_5(MG-D_5) was used as an internal standard to quantitative determination MG, C_(18) chromatographic column(4. 6 mm×250 mm, 5 µm). RESULTS: The method showed a good linearity(R~2=0. 999) in the concentration range of 0. 1-10 µg/mL with the detection limit of 0. 03 µg/mL and the quantitative limit of 0. 1 µg/mL. The intra-and inter-day precisions were 3. 5% and 6. 2%, and the recovery ranged from 98. 9% to 106. 9%(n=7). The detection result by PSI-MS were in agreement with those by liquid chromatography-tandem mass spectrometry(LC-MS/MS), but the run time was only 1 min each sample. CONCLUSION: The method is accurate and reliable. And compared with the conventional LC-MS/MS technology, it is simple, rapid and low-cost.

Rapid analysis of benzoic acid and vitamin C in beverages by paper spray mass spectrometry.

A paper spray mass spectrometry (PS-MS) method has been developed for the rapid quantification of benzoic acid (BA) and vitamin C (VC) in beverages. Using BA-d5 as an internal standard (IS) to analyze BA and VC, the calibration curves ranged from 0.3 to 100 µg/mL for BA and 1 to 100 µg/mL for VC, the linearity was 0.9996 for BA and 0.9973 for VC. The limits of detection (LODs) and limits of quantitation (LOQs) were 0.1 µg/mL and 0.3 µg/mL for BA, 0.3 µg/mL and 1 µg/mL for VC, respectively. The recovery ranged from 91.1 to 106.7% for BA, 92.6 to 108.2% for VC. Compared with HPLC, there is no substantial difference in the quantification of BA and VC in samples, the accuracy was 95.7-102.2%, and the run time is far less than that of the HPLC method. The results indicated that PS-MS is a rapid, environmentally friendly and high-throughput method for the quantification of BA and VC.

Rapid analysis of benzalkonium chloride using paper spray mass spectrometry.

A paper spray mass spectrometry (PS-MS) method for rapid and reliable analysis of benzalkonium chloride (BAC) in compound eye drops and body surface disinfectant was developed. The sample was dropped onto triangular filter paper, and high voltage (3.5kV) was applied to form an electrospray. This method can provide the composition of benzalkonium chloride in samples without pretreatment, solvent or chromatographic separation, and the analysis time is only 10s. The primary homologues C12-BAC, C14-BAC and C16-BAC of benzalkonium chloride were quantitatively analyzed using PS-MS. Samples were subjected to simple dilution and quantified using the internal standard method. Ion trap mass spectrometry was scanned using SIM mode. The linear ranges of C12-BAC, C14-BAC and C16-BAC were 1-100µgmL-1; the linear regression coefficients were 0.998-0.999; the detection limits (LODs) were 0.1µgmL-1; the limit of quantifications (LOQs) was <1µgmL-1, and the method validation indicated that the method precision and accuracy were good. Compared with HPLC-UV methods, there was no significant difference in the quantitative determination of the actual samples, but the analysis time for PS-MS is shorter (2min). In addition, reagent consumption in PS-MS is small, and no chromatographic separation is needed, suggesting that PS-MS is especially suitable for high-throughput analysis.