Simultaneous determination of flavonoids and anthraquinones in honey by using SPE-CE-LIF.

Based on advantages of capillary electrophoresis (CE), a new solid-phase extraction (SPE) coupled with CE has been developed for preconcentration, enrichment and determination of anthraquinones and flavonoids (rutin, emodin, quercetin, 1,8-dihydroxyanthraquinone) in honey. The environmental-friendly chitin activated after an easy processing is selected as the adsorbent to enrich analytes. Then, chitin was filled into the filter as the solid phase. To improve the extraction effect, some key parameters of extraction were optimized. Under the optional extraction conditions, the chitin showed excellent adsorption capacity and selectivity over rutin, emodin, quercetin, and 1,8-dihydroxyanthraquinone, with enrichment factors reaching 5 folds. The CE coupled with fluorescence detection was used for the detection. Results prove the method is simple, fast, and highly sensitive, with the limit of detection (LOD) is 3.00-200.0 ng/mL; the recovery is 90.0-107.0%, and relative standard deviation of (RSD) is 1.8-8.3%.

Sensitive analysis of doxorubicin and curcumin by micellar electromagnetic chromatography with a double wavelength excitation source.

Doxorubicin has been extensively used to treat cancers, and there are recent findings that the anticancer activities can be enhanced by curcumin. Although the two compounds have native fluorescence, they can hardly be quantified directly simultaneously using the laser-induced fluorescence (LIF) detection method. To avoid complex fluorescence derivatization and introduction of interfering components, a highly sensitive double wavelength excitation source LIF (D-W-Ex-LIF) detector composed of a 445-nm and 488-nm commercial laser diode was constructed to detect them simultaneously. Rhodamine 6G was selected as an internal standard, because its fluorescence can be excited at 445 nm and 488 nm. The native fluorescence of doxorubicin and curcumin and their resolution were enhanced by introducing mixed micelles. The optimal electrophoretic separation buffer was 10 mM borate buffer containing 20 mM Triton X-100, 5 mM sodium dodecyl sulfate, and 30% (v/v) methanol at pH 9.00. Therefore, the developed method was specific, accurate, and easily operable. Its limits of detection for doxorubicin and curcumin in human urine samples were 4.00 × 10-3 and 1.00 × 10-2 µg/mL, respectively, and the limits of quantification were 1.00 × 10-2 and 3.00 × 10-2 µg/mL, respectively. The recoveries were 94.9-109.1%. Graphical abstract.

A simple and highly sensitive masking fluorescence detection system for capillary array electrophoresis and its application to food and medicine analysis.

A high sampling rate, good stability, high throughput masking fluorescence detection system with easy positioning of each channel for capillary array electrophoresis was prepared and studied. A special mask combined with convex lenses was designed to modulate signals, without using any extra device to position each channel. The signal of each channel was detected by a photomultiplier tube, classified and saved by software. The design was used to evidently reduce the rotational vibration of optical components and to stabilize the system, so a high sampling rate was obtained by increasing the DC motor speed. To improve the optical system, optical fibers instead of conventional bulky optical components were used to transmit optical signal and to collect fluorescences in multiple directions, which greatly raised the sensitivity. Other important parameters including sampling rate, rotating speed and driven voltage laser diode (LDs) have also been investigated. Under optimal conditions, the performance of the detection system was evaluated. This novel system had a well-designed structure, and allowed independent multiple capillary operations and easy microanalysis. Its limit of detection for rhodamine 6G was 2.0 × 10-2 µg/mL.