Genome analysis of Salmonella phage vB_SalM_8-19 (genus Rosemountvirus).

This work describes the characterization and genome annotation of Salmonella phage vB_SalM_8-19 (referred to as 8-19) isolated from sewage samples collected in a pig farm in Jilin, China. This phage was capable of infecting 60% Salmonella strains in our lab stock. The genome of phage 8-19 is composed of linear double-stranded DNA that is 52,648 bp in length with a G + C content of 46.02%; containing 74 ORFs and no tRNA genes. In October 2019, phylogenetic analyses indicated that phage 8-19 might belong to a novel cluster among the other similar phages which have not been specifically classified within some new genus in family Myoviridae. Recently, the International Committee on Taxonomy of Viruses (ICTV) defined phage 8-19 and its related phages as genus Rosemountvirus, family Myoviridae. This new genus, known as Rosemountvirus, is rarely reported in the literature.

Attitudes of the patients and their families towards cancellation of anesthesia and surgery in hypertensive patients.

BACKGROUND: Pre-operative hypertension which is a common cause for cancellation of scheduled surgery is not merely a medical consideration. Understanding the patients' and their families' attitudes towards this issue may help develop an efficient system of qualitative management and promote the utilization of medical resources in a hospital. METHODS: Questionnaire of the study regarding the attitudes towards pre-operative hypertension and cancellation of scheduled surgery was answered through interviews by the patients and families admitted in a rural hospital in early 2003. RESULTS: A total of 189 people were interviewed. Over 42% of interviewees said that cancellation of scheduled surgery in hypertensive patients was acceptable. Nearly 43% felt that such a cancellation did not cause any loss to patient. However, less than 12% of respondents could accept the occurrence of a post-operative cardiovascular complication as a reasonable event even though most of them acknowledged that a hypertensive patient was not a good risk for surgery. If any loss or complication occurred, more than half of them thought that it was the surgeon's fault. CONCLUSIONS: Most patients and families in this rural area accept case cancellation rather than tempt the suffering of a post-operative cardiovascular complication. Developing a standard guideline for postponement of anesthesia and surgery in hypertensive patients may help reduce the variability in practising and management, with resultant increase of operating suite efficiency.