Well-designed two-fold crosslinked biological valve leaflets with heparin-loaded hydrogel coating for enhancing anticoagulation, endothelialization, and anticalcification.

Commercial biological valve leaflets (BVLs) crosslinked with glutaraldehyde (GA) are at risk of accelerating damage and even failure, owing to the high cell toxicity of GA, acute thrombosis, and calcification in clinical applications. In this study, a novel joint strategy of double crosslinking agents (dialdehyde pectin (AP) and carbodiimide) and heparin-loaded hydrogel coating was developed, endowing BVLs with excellent mechanical properties and multiple performances. Herein, AP played two essential roles, the crosslinking agent and the main component of the hydrogel coating. Both experimental and theoretical results indicated that the mechanical properties and stability of double-crosslinked BVLs were comparable to those of GA, and heparin loaded in hydrogel coating could improve the hemocompatibility of AP + EDC/NHS-PP. Further, cytocompatibility and in vivo tests showed that compared with GA-PP, AP + EDC/NHS + CS + Hep-PP has exhibited good endothelialization ability, mild immune response and anti-calcification performance and therefore prompts it to be an extremely valuable candidate for more durable and multifunctional BVLs.

A Comparative Study Between Porcine Peritoneum and Pericardium as Cardiovascular Material.

Decellularized porcine pericardium has many applications in the cardiovascular field for its excellent properties. The peritoneum is a single-layer bio-dialysis membrane with many similarities and differences in physical characteristics, biochemical composition, and structure to the pericardium. The limited available literature suggests that, similar to the pericardium, the peritoneum has good application potential in the field of cardiovascular substitute materials. This research focused on comparing the differences between decellularized peritoneum and decellularized pericardium in microstructure, biochemical composition, mechanical properties, hemocompatibility, in vitro enzymatic degradation, in vitro calcification, cytocompatibility, and other vital indicators. The peritoneum was consistent with pericardium in terms of fibrous structure, hemocompatibility, in vitro calcification, and cytocompatibility. The peritoneal elastic fiber content (219 µg/mg) was significantly higher than that of the pericardium (66 µg/mg), resulting in two to three times higher maximum load (21.1 N) and burst pressure (1309 mmHg), and better performance than the pericardium in terms of in vitro resistance to enzymatic degradation. In the cardiovascular field, decellularized peritoneum can be used as vascular substitute material. Impact statement There are many similarities between the embryonic origin and morphological structure of the porcine peritoneum and the porcine pericardium, but little research has been done on the use of the porcine peritoneum as a biomaterial. In this compared research, we showed that porcine peritoneum had better resistance to enzymatic degradation, better stretching, and more suitable burst pressure for being used as vascular substitute material. This research is the first to describe the structural composition of porcine peritoneum and its advantageous properties as a cardiovascular material.