RESUMO
Polymeric nanocomposites with graphene-based nanocarbons (GNCs) have been extensively studied with emphasis on the percolation of nanofillers toward electrical, rheological, and mechanical reinforcement. In this study, we report an unusual indirect reinforcing phenomenon of highly defective GNCs dispersed in the poly(vinyl chloride) (PVC) matrix via densification of the polymer packing originating from nanoscale confinement. Herein, chemically reduced graphene oxide nanoribbons (C-rGONRs) are employed as a nanofiller. The inclusion of defective and oxygen-functionalized C-rGONRs resulted in a dramatic densification of the PVC host with extremely low C-rGONR loading, largely exceeding the theoretical calculation from a rule of mixture. Along with the densification, the glass transition temperature of PVC also increased by 28.6 °C at 0.1 wt% filler loading. Remarkably, the oxygen barrier property and mechanical toughness under tension for the PVC/C-rGONR nanocomposite were the maximum when the greatest densification occurred. The structure-property relationship of the nanocomposites has been discussed with an emphasis on the nanoscale confinement phenomenon.
RESUMO
We report microporous carbon nanosheets containing numerous redox active heteroatoms fabricated from exfoliated waste coffee grounds by simple heating with KOH for pseudocapacitive charge storage. We found that various heteroatom combinations in carbonaceous materials can be a redox host for lithium ion storage. The bio-inspired nanomaterials had unique characteristics, showing superior electrochemical performances as cathode for asymmetric pseudocapacitors.
RESUMO
OBJECTIVES: This study aimed to analyse demographic profiles and pre-injection stroboscopic findings for patients with unilateral vocal fold paralysis, to investigate possible predictive factors for voice outcomes of injection laryngoplasty. MATERIALS AND METHODS: Fifty-nine unilateral vocal fold paralysis patients underwent vocal fold augmentation, using transcutaneous Artecoll (polymethyl methacrylate microspheres plus bovine collagen) injection into the paralysed vocal fold via the cricothyroid space. Three months later, patients were divided into improved (n = 44) and unimproved (n = 15) groups, using the perceptual grade-roughness-breathiness-asthenia-strain scale, and their clinical characteristics and pre-operative stroboscopic findings compared. RESULTS: The improved group were significantly younger than the unimproved group (p = 0.000). The size of the posterior gap on phonation was closely associated with the outcome of injection laryngoplasty (p = 0.015). CONCLUSION: Younger patients with a smaller posterior glottic gap on phonation can be expected to have a more favourable outcome following injection laryngoplasty for correction of glottic insufficiency due to unilateral vocal fold paralysis.
Assuntos
Glote/patologia , Laringoplastia/métodos , Paralisia das Pregas Vocais/cirurgia , Distúrbios da Voz/cirurgia , Qualidade da Voz/fisiologia , Adulto , Fatores Etários , Idoso , Animais , Bovinos , Colágeno/administração & dosagem , Colágeno/uso terapêutico , Feminino , Glote/fisiopatologia , Glote/cirurgia , Humanos , Injeções , Modelos Logísticos , Masculino , Microesferas , Pessoa de Meia-Idade , Fonação/fisiologia , Polimetil Metacrilato/administração & dosagem , Polimetil Metacrilato/uso terapêutico , Estroboscopia , Resultado do Tratamento , Paralisia das Pregas Vocais/fisiopatologia , Distúrbios da Voz/fisiopatologiaRESUMO
In this paper we studied the potential of nanocrystalline palladium particle production using Cinnamom zeylanicum bark extract (CBE) as the biomaterial for the first time. We studied the effects of biomaterial dosage, pH and temperature on nanoparticle formation; none of these factors had a major effect on the size and shape of the nanoparticles formed. Transmission electron microscopy (TEM) observations confirmed the synthesis of nano-sized palladium particles. More or less uniformly sized palladium nanoparticles were synthesized with an average size ranging from 15 to 20 nm. It was found that the zeta potential of these formed palladium nanoparticles was negative, and that it increased with an increase in pH. Energy dispersive X-ray (EDX) analysis results confirmed the significant presence of palladium. Of the palladium ions, 60% were reduced to a zero valent form by CBE. Terpenoids are believed to play an important role in palladium nanoparticle biosynthesis through the reduction of palladium ions. Currently, however, the exact mechanism for the synthesis of palladium nanoparticles is unclear. Our protocol for the phyto-synthesis of palladium nanoparticles under moderate pH and room temperature offers a new means to develop environmentally benign nanoparticles.
Assuntos
Dióxido de Carbono/química , Cinnamomum zeylanicum/metabolismo , Etilenoglicol/química , Paládio/química , Casca de Planta/metabolismo , Catálise , Físico-Química/métodos , Cristalização , Microscopia Eletrônica de Varredura/métodos , Nanopartículas/química , Tamanho da Partícula , Análise Espectral Raman/métodos , Temperatura , Água/química , Difração de Raios XRESUMO
The exploitation of various plant materials for the biosynthesis of nanoparticles is considered a green technology as it does not involve any harmful chemicals. The present study reports the synthesis of silver (Ag) nanoparticles from silver precursor using the bark extract and powder of novel Cinnamon zeylanicum. Water-soluble organics present in the plant materials were mainly responsible for the reduction of silver ions to nano-sized Ag particles. TEM and XRD results confirmed the presence of nano-crystalline Ag particles. The pH played a major role in size control of the particles. Bark extract produced more Ag nanoparticles than the powder did, which was attributed to the large availability of the reducing agents in the extract. Zeta potential studies showed that the surface charge of the formed nanoparticles was highly negative. The EC(50) value of the synthesized nanoparticles against Escherichia coli BL-21 strain was 11+/-1.72 mg/L. Thus C. zeylanicum bark extract and powder are a good bio-resource/biomaterial for the synthesis of Ag nanoparticles with antimicrobial activity.
Assuntos
Antibacterianos/biossíntese , Cinnamomum zeylanicum/química , Química Verde/métodos , Nanopartículas Metálicas/química , Casca de Planta/metabolismo , Extratos Vegetais/metabolismo , Prata/farmacologia , Antibacterianos/farmacologia , Materiais Biocompatíveis/farmacologia , Cristalização , Escherichia coli/efeitos dos fármacos , Escherichia coli/crescimento & desenvolvimento , Concentração de Íons de Hidrogênio/efeitos dos fármacos , Nanopartículas Metálicas/ultraestrutura , Testes de Sensibilidade Microbiana , Pós , Análise Espectral , Difração de Raios XRESUMO
Ginsan is a polysaccharide extracted from Panax ginseng that is known to have multiple immunomodulatory effects. This study evaluates the chemoprotective effect of ginsan on normal mice and the adjuvant effect on tumor bearing mice in combination with cyclophosphamide (CP). Ginsan (100 mg/kg) was injected 24 h before or after a sublethal dose of a CP treatment. The mice pre-treated with ginsan all died within 10 days whereas up to 53 percent of the mice post-treated with ginsan increased survival to day 30 compared with only 10 percent in the CP alone treated group on day 30. The post-treatment of ginsan accelerated the recovery of the bone marrow cells and blood neutrophils by approximately 1.3- and 1.75-fold compared to CP treated control mice at 5 days after CP administration, respectively. These marked differences in activity between the pre- and post-treatment of ginsan with CP was clarified by examining the mRNA expression levels of several cytokines in spleen cells and the self-renewal potential of hematopoietic progenitor cells, CFU-s. The post-treatment with ginsan increased the mRNA expression levels of TNF-alpha, IL-1beta, IL-6, SCF, and GM-CSF with respect to that of the CP alone or ginsan pre-treated group. Similarly, the number of CFU-s was significantly higher in the mice post-treated with ginsan. The inhibition of tumor growth and survival elongation was also observed when ginsan was administered 24 h after the CP treatment. These results show that the post-treatment with ginsan had an immunomodulating and adjuvant effect in combination with CP, which indicates its wide applications in reducing the adverse effects of chemotherapy and improving the general conditions of patients.
Assuntos
Adjuvantes Imunológicos , Antineoplásicos Alquilantes/uso terapêutico , Carcinoma Pulmonar de Lewis/tratamento farmacológico , Ciclofosfamida/uso terapêutico , Polissacarídeos , Adjuvantes Imunológicos/administração & dosagem , Adjuvantes Imunológicos/isolamento & purificação , Adjuvantes Imunológicos/uso terapêutico , Animais , Antineoplásicos Alquilantes/administração & dosagem , Antineoplásicos Alquilantes/efeitos adversos , Contagem de Células Sanguíneas , Células da Medula Óssea/efeitos dos fármacos , Células da Medula Óssea/imunologia , Carcinoma Pulmonar de Lewis/sangue , Carcinoma Pulmonar de Lewis/imunologia , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Ciclofosfamida/administração & dosagem , Ciclofosfamida/efeitos adversos , Citocinas/biossíntese , Esquema de Medicação , Feminino , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos C57BL , Panax/química , Polissacarídeos/administração & dosagem , Polissacarídeos/isolamento & purificação , Polissacarídeos/uso terapêutico , RNA Mensageiro/biossíntese , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Baço/citologia , Baço/efeitos dos fármacos , Baço/metabolismo , Células-Tronco/citologiaRESUMO
N-acetylphytosphingosine (NAPS), a sphingolipid derivative, is one of the well-known signal molecules that mediates various cellular functions, including cell growth, differentiation, and apoptosis. In this study, we demonstrated that NAPS induces apoptosis of Jurkat cells by activating Bak, but not Bax, which are both members of a proapoptotic subfamily of the Bcl-2 proteins. NAPS activated caspase-8 in a FADD-independent manner, but the lack of caspase-8 did not suppress the activation of caspase-3 and -9 and cell death, indicating that caspase-8 activation does not play an important role in NAPS-induced cell death. The overexpression of Bcl-xL, an anti-apoptotic protein, completely inhibited the activation of the caspases and apoptosis, assuming that NAPS-induced apoptosis was initiated by the mitochondria. The expression levels of pro- and anti-apoptotic Bcl-2 family members were not changed by the NAPS treatment. However, Bad was translocated from the cytosol into the mitochondria, where it bound to Bcl-xL, and Bak was dissociated from Bcl-xL and conformationally changed. Taken together, these findings indicate that NAPS induced apoptosis of Jurkat cells in a mitochondria-dependent manner that was controlled by the translocation of Bad and the conformational change in Bak.
Assuntos
Antineoplásicos/farmacologia , Apoptose , Esfingolipídeos/farmacologia , Proteína Killer-Antagonista Homóloga a bcl-2/metabolismo , Proteínas Adaptadoras de Transdução de Sinal/metabolismo , Caspase 8 , Inibidores de Caspase , Caspases/metabolismo , Proteína de Domínio de Morte Associada a Fas , Humanos , Células Jurkat , Mitocôndrias/metabolismo , Poli(ADP-Ribose) Polimerases/metabolismo , Conformação Proteica , Proteína Killer-Antagonista Homóloga a bcl-2/química , Proteína de Morte Celular Associada a bcl/metabolismo , Proteína bcl-X/metabolismoRESUMO
Biomass waste, mainly Corynebacterium glutamicum, is generated from large-scale lysine fermentation process. In this study, protonated C. glutamicum biomass was evaluated as a biosorbent for the removal of lead from synthetic wastewater. As Pb2+ were bound to the biomass, the solution pH deceased, indicating that protons in the biomass were exchanged with lead ions. The Corynebacterium biomass bound Pb2+ at up to 2.74 mmol g(-1) at pH 5, where lead does not precipitate. Compared with other biosorbents and conventional sorbents, such as natural zeolite, activated carbon and synthetic ion exchange resin, the protonated C. glutamicum biomass was considered to be a useful biomaterial for lead biosorption.
Assuntos
Corynebacterium/metabolismo , Chumbo/química , Lisina/química , Adsorção , Biomassa , Cálcio/química , Carbono/química , Fermentação , Concentração de Íons de Hidrogênio , Íons , Chumbo/farmacocinética , Magnésio/química , Prótons , Sódio/química , Fatores de Tempo , Zeolitas/químicaAssuntos
Ventrículos do Coração/anatomia & histologia , Insuficiência da Valva Mitral/cirurgia , Complicações Pós-Operatórias/etiologia , Disfunção Ventricular Esquerda/etiologia , Criança , Pré-Escolar , Ecocardiografia , Feminino , Ventrículos do Coração/diagnóstico por imagem , Humanos , Lactente , Masculino , Insuficiência da Valva Mitral/fisiopatologia , Complicações Pós-Operatórias/fisiopatologia , Valor Preditivo dos Testes , Cuidados Pré-Operatórios , Estudos Retrospectivos , Fatores de Risco , Sístole , Disfunção Ventricular Esquerda/fisiopatologiaRESUMO
AIMS: The aim of this study was to investigate the therapeutic effect of recombinant human erythropoietin (rHuEpo) on anaemia with erythropoietin deficiency in diabetic patients. METHODS: Twenty diabetic patients with anaemia and Epo deficiency were enrolled. All patients were treated with rHuEpo (Epokine; 4000 U/day s.c., three times a week) for 8 weeks. RESULTS: The responder group (n = 14) had significant increments in haemoglobin compared with the non-responder group (n = 6) (P < 0.05). No significant differences were found between the responder and non-responder groups in terms of duration of diabetes mellitus, serum creatinine level, 24-h urine albumin excretion rates, frequency of diabetic microangiopathy, or HbA1c. There was no difference between the two groups in terms of serum iron and total iron-binding capacity (TIBC). Serum ferritin level was significantly higher in the responder group than in the non-responder group (240.3 +/- 108.4, 25.8 +/- 3.0 micro g/l, P < 0.05), as was transferrin saturation (32.7 +/- 7.9%, 21.2 +/- 5.3%, P < 0.05). CONCLUSIONS: rHuEpo could be useful in the treatment of anaemia with erythropoietin deficiency in diabetic patients, and the degree of iron storage and functional iron deficiency might be the main cause of hyporesponsiveness to rHuEpo.
Assuntos
Anemia/tratamento farmacológico , Complicações do Diabetes , Eritropoetina/deficiência , Eritropoetina/uso terapêutico , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Proteínas RecombinantesRESUMO
Factors affecting the astaxanthin production by a unicellular green alga, Haematococcus pluvialis UTEX 16, were evaluated with sequential fractional factorial design. To simulate an actual production mode, a two-stage process was adapted for astaxanthin production: the alga was first cultivated under vegetative growth conditions, and then astaxanthin production was induced by applying various induction methods. A high dose of irradiation was most effective for the production of astaxanthin both in weight (mg/g) and in cellular (pg/cell) contents. A combination of nitrogen deficiency and acetate addition also significantly increased the astaxanthin content of cells on a dry weight basis. Meanwhile, the acetate addition alone increased only the cellular content of astaxanthin. Although the addition of ferrous ion alone had a negative effect on the weight content of astaxanthin, simultaneous addition of ferrous ion and acetate was effective for increasing the cellular content of astaxanthin.
Assuntos
Clorófitas/crescimento & desenvolvimento , Clorófitas/metabolismo , beta Caroteno/análogos & derivados , beta Caroteno/biossíntese , Acetatos/farmacologia , Biomassa , Ferro/farmacologia , Luz , Modelos Teóricos , Nitrogênio/farmacologia , Projetos de Pesquisa , XantofilasRESUMO
The aim of the present research was conducted to elucidate anti-septicaemic effect of a polysaccharide (PS) isolated from Panax ginseng C.A. Meyer (Araliaceae) by nitric oxide production from stimulated macrophage. In vitro assays for the activity measurement of PS, NO production test with Greiss reagent, phagocytic activity test using zymosan and cytokines production test using ELISA kit were also conducted. In vivo anti-septicaemic activity was assessed by using C57BL/6J mice. This was done with Staphylococcus aureus infection test. PS used at 0.025 mg/kg concentration showed a potent anti-septicaemic activity (80%, survival). However, it did not directly inhibit S. aureus in a minimum inhibitory concentration (MIC) test, conducted in vitro (data not shown). Nitric oxide production via macrophage activation showed the highest value of 5.5 nmol/ml at 1 microg/ml PS. In in vitro phagocytic activity test, PS at 10 microg/ml concentration showed a potent phagocytic activity for zymosan with 167% of the control. Production of TNF-alpha by macrophage activation at 10 microg/ml of PS was 96% lysis of L929. Also production of IL-1 and IL-6 by stimulation of macrophage with 100 microg/ml PS dose increased to 235 pg/ml and 0.47 ng/ml, respectively. The low mortality of PS treated (0.025 mg/kg) infected mice was concurrent with decreased bacterial content in the blood. Nitric oxide production in S. aureus infected mice whose macrophage was stimulated by PS (0.025 mg/kg) increased approximately 4 times than the untreated S. aureus infected group at 24 and 48 h incubation. In the PS treated (0.025 mg/kg) group, the intracellular concentration of S. aureus in macrophages decreased approximately by 50%, compared with the untreated group. Combine treatment with PS (0.025 mg/kg body weight) and vancomycin (10 mg/kg B.W.) resulted in 100% survival of the animals, whereas only 67% or 50% of the animals survived, respectively, when treated with PS or vancomycin alone. These results suggest that PS from Panax ginseng possess a potent anti-septicaemic activity by stimulating macrophage and a potentiality as an immunomodulator against sepsis occurred by Staphylococcus aureus.
Assuntos
Ativação de Macrófagos/efeitos dos fármacos , Panax/química , Polissacarídeos/farmacologia , Polissacarídeos/uso terapêutico , Sepse/tratamento farmacológico , Sepse/imunologia , Animais , Antibacterianos/farmacologia , Citocinas/biossíntese , Citocinas/imunologia , Relação Dose-Resposta a Droga , Feminino , Macrófagos Peritoneais/efeitos dos fármacos , Macrófagos Peritoneais/imunologia , Macrófagos Peritoneais/metabolismo , Macrófagos Peritoneais/microbiologia , Camundongos , Camundongos Endogâmicos C57BL , Testes de Sensibilidade Microbiana , Óxido Nítrico/metabolismo , Nitritos/metabolismo , Fagocitose/efeitos dos fármacos , Infecções Estafilocócicas/tratamento farmacológico , Infecções Estafilocócicas/imunologia , Staphylococcus aureus/efeitos dos fármacos , Staphylococcus aureus/fisiologia , Fatores de Tempo , Vancomicina/farmacologiaRESUMO
Electroacupuncture (EA) has been reported to modulate natural killer cell (NK cell) activities. Also it is well known that hypothalamus directly mediates the effects of EA on analgesia. Especially lateral hypothalamic area (LHA) is related to splenic NK cell activities. In order to investigate the relationship between hypothalamus and effects of EA on NK cell activity, lesions have been made bilaterally at LHA of Spraque-Dawley rats. Subsequently, NK cell cytotoxities of normal and lesioned rats were measured with (51)Cr release immunoassay after EA stimulation for 2 and 14 days. NK cell activity of EA group was significantly higher than sham group. In addition, lesions abolished effects of EA on NK cell activity. These results strongly suggest that LHA is closely related to increase of NK cell activity induced by EA.
Assuntos
Eletroacupuntura , Região Hipotalâmica Lateral/imunologia , Células Matadoras Naturais/imunologia , Animais , Radioisótopos de Cromo , Masculino , Ratos , Ratos Sprague-Dawley , Baço/citologia , Baço/imunologiaRESUMO
Ketosteroid isomerase (KSI) from Pseudomonas putida biotype B is a homodimeric enzyme catalyzing an allylic rearrangement of Delta5-3-ketosteroids at rates comparable with the diffusion-controlled limit. The tyrosine triad (Tyr14.Tyr55.Tyr30) forming a hydrogen-bond network in the apolar active site of KSI has been characterized in an effort to identify the roles of the phenyl rings in catalysis, stability, and unfolding of the enzyme. The replacement of Tyr14, a catalytic residue, with serine resulted in a 33-fold decrease of kcat, while the replacements of Tyr30 and Tyr55 with serine decreased kcat by 4- and 51-fold, respectively. The large decrease of kcat for Y55S could be due to the structural perturbation of alpha-helix A3, which results in the reorientation of the active-site residues as judged by the crystal structure of Y55S determined at 2.2 A resolution. Consistent with the analysis of the Y55S crystal structure, the far-UV circular dichroism spectra of Y14S, Y30S, and Y55S indicated that the elimination of the phenyl ring of the tyrosine reduced significantly the content of alpha-helices. Urea-induced equilibrium unfolding experiments revealed that the DeltaG(U)H2O values of Y14S, Y30S, and Y55S were significantly decreased by 11.9, 13.7, and 9.5 kcal/mol, respectively, as compared with that of the wild type. A characterization of the unfolding kinetics based on PhiU-value analysis indicates that the interactions mediated by the tyrosine triad in the native state are very resistant to unfolding. Taken together, our results demonstrate that the internal packing by the phenyl rings in the active-site tyrosine triad contributes to the conformational stability and catalytic activity of KSI by maintaining the structural integrity of the alpha-helices.
Assuntos
Pseudomonas putida/enzimologia , Esteroide Isomerases/metabolismo , Tirosina/metabolismo , Substituição de Aminoácidos , Sítios de Ligação , Catálise , Dicroísmo Circular , Cristalização , Cristalografia por Raios X , Estabilidade Enzimática , Cinética , Modelos Moleculares , Mutação , Dobramento de Proteína , Estrutura Secundária de Proteína , Esteroide Isomerases/química , Esteroide Isomerases/genética , Tirosina/químicaRESUMO
Biosorption has attracted attention as a cost-effective means for the treatment of metal-bearing wastewater. However, the mechanism of metal binding is not clearly understood, and consequently, modeling of the biosorption performance is still raising debates. In this study, the biosorption of trivalent chromium was investigated with protonated brown alga Ecklonia biomass as a model system. Titration of the biomass revealed that it contains at least three types of functional groups. The Fourier transform infrared spectrometry showed that the carboxyl group was the chromium-binding site within the pH range (pH 1-5) used in this study, where chromium does not precipitate. The pK value and the number of carboxyl groups were estimated to be 4.6 +/- 0.1 and 2.2 +/- 0.1 mmol/g, respectively. The equilibrium sorption isotherms determined at different solution pH indicated that the uptake of chromium increased significantly with increasing pH. A model for the description of chromium biosorption was developed incorporating the hydrolysis reactions that chromium undergoes in the aquatic phase. The model was able to predict the equilibrium sorption experimental data at different pH values and chromium concentrations. In addition, the speciation of the binding site as a function of the solution pH was predicted using the model in order to visualize the distribution of chromium ionic species on the binding site.
Assuntos
Cromo/farmacocinética , Phaeophyceae/metabolismo , Alga Marinha/metabolismo , Absorção , Biomassa , Concentração de Íons de Hidrogênio , Resíduos Industriais/análise , Modelos Biológicos , Eliminação de Resíduos Líquidos/métodos , Purificação da Água/métodosRESUMO
The slurry bioreactor system is an effective means for treating highly saline food wastes, which may not be recycled as composts. The effect of aeration rate was investigated in a slurry bioreactor as a major factor affecting the slurry-phase decomposition of food wastes. The aeration rate affected significantly the decomposition performance and the composition profiles of the liquid and solid phases. The decomposed carbon was almost linear with oxygen consumption, indicating that the slurry-phase decomposition of food wastes was limited by oxygen transfer. The oxygen requirement for decomposing 1 g organic carbon in food wastes was estimated to be 61.5 g O(2).
Assuntos
Reatores Biológicos , Resíduos de Alimentos , Oxigênio/química , Eliminação de Resíduos/métodos , Concentração de Íons de Hidrogênio , Nitratos/análise , Consumo de Oxigênio , Fosfatos/análiseRESUMO
A quantitative description of light attenuation in microalgal suspensions is a prerequisite for kinetic modeling of microalgal photosynthesis and/or growth activity depending upon the light distribution inside photobioreactors. In this study, the light attenuation coefficients in Chlorella vulgaris suspensions were theoretically calculated from light absorption spectra and spectral irradiances of various light sources. By using this method, errors occurring in the direct measurement of the attenuation coefficients can be avoided. The obtained light attenuation coefficients were used for evaluating light attenuation models such as the Beer-Lambert, Cornet, and hyperbolic models. Furthermore, advantages and disadvantages of these models are discussed with respect to prediction of performance, mechanistic background, and usefulness for further application to calculation of the light distribution inside photobioreactors.
Assuntos
Chlorella/crescimento & desenvolvimento , Chlorella/metabolismo , Luz , Reatores Biológicos , Matemática , Modelos Biológicos , Fotossíntese , Espalhamento de Radiação , EspectrofotometriaRESUMO
BACKGROUND: The Lecompte procedure for correcting transposition of the great arteries has an advantage because it obviates the need for an extracardiac conduit for the reconstruction of the pulmonary outflow tract. In this study, we evaluated the effectiveness and the application of the Lecompte procedure based on our experiences. METHODS: A retrospective review was conducted of the records of 45 patients who underwent the Lecompte procedure during the past 11 years to achieve direct right ventricle to pulmonary artery continuity. Mean age at operation was 2.4+/-1.7 years (range 3.5 months to 6.9 years). The diagnoses involved anomalies of the ventriculoarterial connection with ventricular septal defect and pulmonary outflow tract obstruction, such as transposition of the great arteries, double-outlet right ventricle, and double-outlet left ventricle. RESULTS: Early mortality was 4.4% (2 of 45 patients) and late mortality was 4.7% (2 of 43). The mean follow-up was 4.9+/-3.1 years. Fourteen patients (34.1% of survivors, n = 41) had pulmonary stenosis (pressure gradient above 30 mm Hg), the main reason for which was a calcified monocusp valve (n = 10, 71.4%). Eight of 45 patients (17.8%) underwent reoperation: 2 for residual ventricular septal defect, 1 for recurrent septic vegetation, and 5 for pulmonary stenosis. The cumulative survival rates were 91.1%+/-4.2% at 10 years. The actuarial probabilities of freedom from reoperation for pulmonary stenosis were 93.8%+/-4.3% and 71.4%+/-11.8% at 5 and 10 years, respectively. CONCLUSIONS: Our review suggests that the Lecompte procedure is an effective treatment modality for anomalies of the ventriculoarterial connection with ventricular septal defect and pulmonary outflow tract obstruction. Repair in early age is possible with acceptable morbidity and mortality, but recurrent right ventricular outflow tract obstruction caused by degeneration of the monocusp valve is a problem that needs resolution.
Assuntos
Cardiopatias Congênitas/cirurgia , Ventrículos do Coração/anormalidades , Artéria Pulmonar/anormalidades , Análise Atuarial , Criança , Pré-Escolar , Feminino , Cardiopatias Congênitas/mortalidade , Ventrículos do Coração/cirurgia , Humanos , Lactente , Masculino , Complicações Pós-Operatórias/mortalidade , Artéria Pulmonar/cirurgia , Estudos Retrospectivos , Taxa de SobrevidaRESUMO
To investigate an association between surface electrocardiographic (ECG) parameters and sustained ventricular tachycardia (VT) in children after repair of congenital heart disease (CHD), data were obtained and analyzed in three groups (group I, 7 postoperative patients with episode of sustained VT (4 tetralogy of Fallot (TOF), 2 double outlet right ventricle (DORV), 1 truncus arteriosus); group II, 14 children with postoperative TOF not associated with VT; group III, 14 normal children). Mean age at the onset of sustained VT was 129+/-77 months (range 60-232); mean age at corrective surgery, 44+/-33 months (range 10-102); mean follow-up period after surgery, 84+/-74 months (range 20-185); the duration from repair to the onset of sustained VT, range 1-185 months. Compared to group II and III, group I showed longer QRS duration (group I, 137+/-10 msec; group II, 114+/-22 msec; group III, 65+/-12 msec) and shorter corrected J to Tmax interval (group I, 209+/-24 msec; group II, 272+/-44 msec; group III, 249+/-18 msec). QT and corrected QT, J to Tmax interval, and their dispersions in group I and II are significantly different from those of group III. In conclusion, QRS duration and corrected J to Tmax interval could be helpful to predict ventricular tachycardia in postoperative CHD.
Assuntos
Cardiopatias Congênitas/cirurgia , Taquicardia Ventricular/etiologia , Adolescente , Criança , Pré-Escolar , Eletrocardiografia , Feminino , Cardiopatias Congênitas/fisiopatologia , Humanos , Lactente , MasculinoRESUMO
Interleukin-10 (IL-10) has both inhibitory and stimulatory effects on diverse cell types of the immune system. It inhibits the antigen-presenting capacity of monocytes/macrophages and stimulates T cell proliferation. Although many tumors spontaneously release IL-10, the physiological relevance of this phenomenon to the in vivo antitumor immune response is not known. To elucidate the physiological role of tumor-released IL-10, we used IL-10-specific antisense oligodeoxynucleotides (AS-ODN) for the inhibition of IL-10 production from the tumor cells. Incubation of MOPC 315 plasmacytoma with IL-10 AS-ODN in vitro resulted in inhibition of IL-10 production and also in enhancement of the expression of major histocompatibility complex (MHC) class I, MHC class II, and B7-1 molecules. MOPC 315 cells incubated with IL-10 AS-ODN (MOPC-IL10AS) for 16 h in vitro showed reduced tumorigenicity in Balb/c mice. The mice implanted with MOPC-IL10AS effectively rejected the tumor graft, and showed strong cytotoxic T lymphocyte (CTL) activity against the parental MOPC 315 cells. In addition, MOPC-IL10AS were more effective as stimulator cells in mixed lymphocyte/ tumor cell culture, and as target cells in a CTL assay. These results imply that IL-10 spontaneously released from MOPC 315 cells inhibits their immunogenicity and that the inhibition of IL-10 production by IL-10 AS-ODN may be a way to enhance the host cellular antitumor immune response.