RESUMO
The present study was designed to describe the expression of the glutathione S-transferase/glutathione system in squamous cell carcinoma of the bladder in a population in Egypt. The glutathione-S transferase activity was significantly higher in bladder cancer specimens (n = 40) in comparison with schistosomiasis cystitis tissue (n = 42) (4-fold, p = 1 x 10(-12)) and with healthy control samples (n = 9) (10-fold, p = 1 x 10(-6)). The glutathione content was also significantly higher in bladder cancer than in cystitis tissue (2-fold, p = 8 x 10(-6)) and in control samples (6-fold, 8 x 10(-6)). When control mucosa and cystitis samples were compared, 2-fold increased values were obtained for glutathione-S transferase (p = 4 x 10(-3)) and glutathione (p = 1 x 10(-3)) in schistosomiasis bladder tissue. Results describe an over-expression of glutathione-S transferase and glutathione levels in squamous cell carcinoma of the bladder, and indicate a possible role in chemoresistance to pharmacological therapy.
Assuntos
Carcinoma de Células Escamosas/genética , Transformação Celular Neoplásica/genética , Glutationa Transferase/genética , Glutationa/metabolismo , Esquistossomose Urinária/genética , Neoplasias da Bexiga Urinária/genética , Adulto , Idoso , Carcinoma de Células Escamosas/patologia , Transformação Celular Neoplásica/patologia , Resistencia a Medicamentos Antineoplásicos/genética , Egito , Feminino , Regulação Enzimológica da Expressão Gênica/fisiologia , Humanos , Masculino , Pessoa de Meia-Idade , Esquistossomose Urinária/patologia , Bexiga Urinária/patologia , Neoplasias da Bexiga Urinária/patologiaRESUMO
In this study we show an effect of the glutathione-S-transferase M1 (GSTM1) null phenotype on the risk for squamous cell carcinoma (SCC) of the bladder among male smokers in Egypt, with an adjusted odds ratio of 4.8 (95% confidence interval: 1.06-21.77). However, no overall effect of the GSTM1 null phenotype on the risk for bladder SCC was observed.