Impact of road transportation on physiological and oxidative stress biomarkers in puppies: Beneficial effect of melatonin.

Transportation stress is complex and can affect the welfare and health of animals. The study was aimed at investigating the impact of road transportation on puppies and the beneficial effects of melatonin supplementation. Fourteen (14) apparently healthy Nigerian indigenous breed of dogs (puppies) divided into two groups, group I (control) and group II (pretreated with Melatonin at 10 mg/kg) were transported by road during the hot-dry season. Vital parameters; respiratory rate (RR), pulse rate (PR) and rectal temperature (RT) were taken before, during and immediately after commencement of the journey. Blood sample was also collected from each puppy before and immediately after the journey, part of which was used to analyzed for some hematological parameters and oxidative biomarkers such as the concentration of malondialdehyde (MDA) and reduced glutathione (GSH) and the activities of superoxide dismutase (SOD) and catalase (CAT). The result revealed that road transportation caused moderate to severe stress on the puppies as observed with the discomfort index (DI) (27.75-34.64 oC). The dry-bulb temperature during the study period was 33.23 ± 0.13 oC, while the relative humidity was 73.85 ± 0.60%. The RT, PR and RR also increased significantly (p < 0.05) during and immediately after the transportation compared to before commencement of the transportation. The result further revealed that total leucocyte counts and Neutrophil:Lymphocyte ratio (NLR) were elevated immediately after the transportation. The MDA concentration was also significantly (P < 0.05) elevated immediately after the transportation compared to before commencement in the control group. The activities of SOD and CAT and the concentration of GSH was lowered immediately after the transportation compared to before commencement. Supplementation with melatonin before the transportation ameliorated the stress posed by road transportation on the puppies as can be observed in the lowering of the elevated RT, PR and RR, total leucocyte count, NLR and MDA concentration. Pretreated with melatonin also increased the depleted activities of SOD and CAT, and the concentration of GSH immediately after the transportation. The results showed that road transportation is stressful for puppies and supplementation with melatonin mitigated the stress posed on the puppies, probably through the ability of melatonin to scavenge reactive oxygen species generated during the transportation. The results further showed that melatonin may be used to enhance the welfare of puppies subjected to road transportation under adverse thermal conditions.

Rumination and Activity Patterns in Angus and Angus-Cross Beef Calves: Influences of Sex, Breed, and Backgrounding Diet.

The objectives of this study were to evaluate the influences of sex, breed, and backgrounding diet on rumination and activity patterns in Angus and Angus-cross beef calves; and the daily variations in rumination and activity in Angus and Angus-cross beef calves under different backgrounding systems. A total of 62 freshly weaned calves were vaccinated and randomly stratified by sex (heifers and steers), breed (Angus and Angus × Simmental cross), and assigned randomly to 3 backgrounding treatments for 55 days. The peak values for rumination and activity in heifers, steers, Angus, and Angus × Simmental cross occurred during the dark and light phases of the dark/light cycle, respectively. Beef calves backgrounded on cover crops had higher (p < 0.05) rumination (45.33 ± 1.57 min) compared with calves backgrounded on a perennial pasture (43.96 ± 1.47 min) diet. Similarly, drylot calves (24.16 ± 0.68 min) had higher (p < 0.05) activity compared to perennial pasture (23.49 ± 0.72 min). The results showed that sex and breed did not influence rumination and activity of Angus and Angus-cross beef calves during the study period. We concluded that the time of the day and backgrounding diet influenced rumination and activity patterns of Angus and Angus-cross beef calves.

Daytime Evaluation of Oxidative Stress and Behavioural Parameters of Donkeys (Equus asinus) During The Hot-Dry and Cold-Dry (Harmattan) Season in a Tropical Savannah.

The aim of the study was to evaluate oxidative stress and behavioural responses of donkeys during the cold-dry and hot-dry seasons. Thirty donkeys divided into three groups of ten donkeys each according to their age served as experimental subjects. During each season, behavioural and blood parameters of the donkeys were evaluated three times with an interval of one week at 06:00 h, 12:00 h and 18:00 h (GMT+1). During the hot-dry season, the peak dry-bulb temperature (DBT) value of 36.00 ± 1.00°C was recorded at 12:00 h, indicating that the season induced heat stress. The time spent feeding demonstrated diurnal fluctuations in adult, yearling and foal donkeys, with the highest time occurring at 12:00 h in both seasons. The malondialdehyde (MDA) concentrations in adults (2.34 ± 0.14 nMol/L), yearlings (2.28 ± 0.19 nMol/L) and foals (3.48 ± 0.17 nMol/L) during the hot-dry season were significantly (P < 0.05) higher, when compared to the corresponding values (0.96 ± 0.09, 1.18 ± 0.10 and 1.82 ± 0.10 nMol/L, respectively) recorded during the cold-dry season. At 0.3% NaCl-concentration, the percentage haemolysis obtained during the hot-dry season in adult (90.33 ± 0.49%), yearling (91.24 ± 0.88%) and foal (97.44 ± 0.24%) donkeys were significantly (P < 0.05) higher than the corresponding values (78.53 ± 0.98, 78.67 ± 0.58 and 88.23 ± 0.47%, respectively), recorded during the cold-dry season. In conclusion, the hot-dry season induced heat stress in the donkeys, resulting in a decrease in time spent on feeding and oxidative stress, as evidenced by high erythrocyte MDA concentration and percentage haemolysis.

Comparison of diurnal rectal and body surface temperatures in large white piglets during the hot-dry season in a tropical Guinea savannah.

The aim of the study was to determine the differences in rectal and body surface temperatures and their extent of conformity using digital and infrared thermometers, respectively, in piglets during the hot-dry season in a tropical guinea savannah of Nigeria. Thirty Large White piglets of both sexes, aged 10-14 days, served as the experimental subjects. The rectal and surface body temperatures were recorded concurrently with those of the ambient dry- and wet-bulbs, during the day at 06:00, 09:00, 12:00, 15:00 and 18:00 h (GMT +1). There were significant (P < 0.05) diurnal variations in all body and ambient temperature readings, with the highest values obtained in the afternoon (at 15:00 h GMT + 1). The mean diurnal rectal and body surface temperatures in the piglets at 09:00-18:00 h were significantly higher (P < 0.001) than the corresponding values at 06:00 h. The overall mean rectal temperature (39.00 ± 0.04 °C) was higher (P < 0.01) than body surface temperature recorded for the eye (38.05 ± 0.04 °C), ear (38.10 ± 0.07 °C), head (37.97 ± 0.05 °C), nose (35.68 ± 0.13 °C), scapula (38.16 ± 0.06 °C), thigh (38.00 ± 0.06 °C), back (38.02 ± 0.06 °C) and hoof (36.83 ± 0.07 °C). The largest and smallest mean difference between rectal and body surface temperatures was -3.32 ± 0.12 °C and -0.84 ± 0.06 °C for the temperature of the nose and scapula, respectively. The positive correlation (P < 0.05) between body temperatures (rectal and surface) of the piglets with ambient temperature implied that the later had a tremendous effect on the former. Body surface temperatures at the region of eye, ear, head, nose, scapula, thigh, back and hoof had significantly (P < 0.0001) linear and positive relationships with rectal temperature. In conclusion, the similar diurnal trends, highly significant correlation coefficients and linear relationships between the rectal and body surface temperatures suggest that the later may serve as valid and reliable estimates of the former in piglets.

Protective effect of fisetin against subchronic chlorpyrifos-induced toxicity on oxidative stress biomarkers and neurobehavioral parameters in adult male albino mice.

Chlorpyrifos (CPF), a chlorinated organophosphate insecticide that is widely used in agriculture and public health, has neurotoxic effects in animals. In addition to acetylcholinesterase inhibition, CPF has been shown to induce alterations such as oxidative stress and lipid peroxidation. Fisetin is a dietary flavonol that protects the brain tissue against oxidative stress by modulating the activity of antioxidant enzymes. This study was designed to investigate the protective role of fisetin against brain oxidative damages and neurobehavioral parameters induced by subchronic oral exposure to CPF in albino mice. Adult albino mice (males, n = 32, weighing 20 ~ 25 g) were assigned randomly into 4 groups and treated accordingly for 7 weeks as follows: Group 1(S/OIL): served as the control group and were given 2 ml/kg of soya oil; Group 2 (CPF): received CPF (6.6 mg/kg; 1/5th of the LD50); Group 3 (FIS): fisetin (15 mg/kg) and Group 4 (FIS + CPF): received fisetin at 15 mg/kg, followed by CPF (6.6 mg/kg) 30 min later. Co-treatment with FIS + CPF mitigated the increase in brain malondialdehyde concentration (0.28 ± 0.02 nmol/mg) and orchestrated the increase in the activities of catalase (81.35 ± 7.26 µ/mg), superoxide dismutase (93.03 ± 6.63 IU/mL), glutathione peroxidase (68.76 ± 3.554 nmol/mL) and acetylcholinesterase (11.59 ± 0.72 nmol/min/mL) when compared to the CPF group. The result showed that deficits in motor strength and excitability scores induced by subchronic CPF were mitigated by fisetin administration. It was concluded that fisetin has a protective potential in mitigating against oxidative stress and damages in the brain tissues, induced by subchronic exposure to CPF in adult male albino mice.

Comparison of body temperature in donkeys using rectal digital, infrared, and mercury-in-glass thermometers during the hot-dry season in a tropical savannah.

The study aimed at comparing variations in body temperature values recorded using rectal digital, infrared, and mercury-in-glass thermometers in donkeys during the hot-dry season, prevailing under tropical savannah conditions. Thirty donkeys that served as subjects were divided into three groups of adults, yearlings, and foals. Values of the body temperature of each donkey were recorded bihourly, starting from 06:00 h till 18:00 h, by digital (5-cm depth of insertion), mercury-in-glass (3 cm depth), and infrared thermometers. The values obtained by each type of the thermometer were compared with those recorded using a 15-cm digital probe (Model HI935007, Hanna Instruments, range -50.0 to 150.0°C; accuracy ± 0.2°C) which served as the gold standard. Dry-bulb temperature (34.00 ± 0.50°C), temperature-humidity index (79.65 ± 0.15), and wet-bulb globe temperature (28.00 ± 0.50) index peaked at 14:00 h. The mean body temperatures for rectal probe, digital, mercury-in-glass, and infrared thermometers were 38.35 ± 0.11°C, 37.24 ± 0.04°C, 36.76 ± 0.06°C, and 36.92 ± 0.07°C, respectively. In comparison to the rectal probe, the mean bias for digital (-1.11 ± 0.05°C), mercury-in-glass (-1.59 ± 0.07°C), and infrared thermometers (-1.38 ± 0.07°C) was large. The Passing-Bablok regression plot demonstrated significant deviation from linearity (p < 0.01) when digital, infrared, and mercury-in-glass thermometers were compared to the rectal probe. The area under the curve (AUC) for digital (AUC: 0.7005 ± 0.01 [95%: 0.6853 - 0.7310], infrared (AUC: 0.6711 ± 0.01 [95%: 0.6322 - 0.7100], and mercury-in-glass (AUC: 0.6321 ± 0.01 [95%: 0.6001 - 0.7873] thermometers showed poor accuracy with low sensitivity. In conclusion, the use of digital, mercury-in-glass, and infrared thermometers in recording body temperature in donkeys during the hot-dry season underestimated the values. Their use in measuring body temperature may result in wrong diagnosis, and compromise the control of hyperthermia and diseases associated with thermoregulatory impairments in donkeys.

Effect of the Depth of Insertion of the Thermometer on the Rectal Temperature of Donkeys During the Hot-Dry Season in a Tropical Savannah.

The objective of the present study was to evaluate the effect of the depth of insertion and environmental parameters on the rectal temperature (RT) in donkeys during the hot-dry season in a tropical savannah zone of Nigeria. The experimental subjects were comprised of thirty donkeys divided into three groups based on age: group I, 10 foals (40.67 ± 2.20 kg; 1.50 ± 0.02 months); group II, 10 yearlings (91.53 ± 0.54 kg; 1.51 ± 0.01 years); and group III, 10 adults (140 ± 0.71 kg; 8.03 ± 0.06 years). Each group was divided into 5 male and 5 female donkeys. Measurements of the RT were recorded with a digital thermometer probe (model HI935007, Hanna Instruments), which was inserted into the rectum at varying depths of 3.5, 7, 10.5, and 14 cm in the same animal in each group. There was a gradual increase in the RT in donkeys as the depth of insertion was increased from 3.5 cm (36.60°C) to 14 cm (38.40°C). Data obtained from the study were subjected to repeated-measures analysis of variance, followed by Tukey's post-hoc test to compare mean values between different depths of RT measurements. Overall, there was a variation in the RT by the depth of insertion with the shallow depth of 3.5 cm having a lower RT than the depths of 7, 10.5, and 14 cm. The variation of the RT observed in donkeys showed that there is need to standardize the probe-insertion depth in veterinary clinical practice for accurate measurement of the RT in donkeys in the Northern Guinea savannah zone of Nigeria.

Changes in feed consumption and water intake among broiler chickens subjected to melatonin treatment during the hot-dry season.

The experiment was conducted to evaluate changes in feed consumption and water intake among broiler chickens subjected to melatonin treatment during the hot-dry season. A total of 300 broiler chicks were selected and assigned into three groups, by simple random sampling, comprising 100 chicks each: group I was exposed to natural photoperiod of about 12-h light and 12-h darkness cycle (12D/12L), without melatonin supplementation; group II was kept under 24-h continuous lighting (CL), without melatonin supplementation; and group III was raised under 24-h CL and administered daily with melatonin orally at 0.5 mg/kg (CL + MEL). Live weight (LW), feed consumption, and water intake for each group were obtained at weekly intervals over a period of 8 weeks. On day 42 of age, the LW of 2420 ± 50 g/bird was obtained in group III administered with melatonin (CL + MEL), while LW values recorded in the 12D/12L and CL groups not administered with melatonin were 1470.00 ± 30.00 and 1907.00 ± 38.00 g/bird, respectively. The mean weight gain in CL + MEL (345.00 ± 21.01 g) was significantly (P < 0.05) higher than those of the 12D/12L (244.99 ± 18.67 g) and CL (307.48 ± 18.14 g) groups. Feed consumptions were significantly (P < 0.05) different in all the groups. Group II, raised on CL without melatonin supplementation, had the highest feed consumption value of 25.14 ± 0.51 g/bird from day 14, and attained the peak value of 206.77 ± 7.82 g/bird at day 56. The highest overall amount of water intake was recorded in the melatonin-treated group. In conclusion, melatonin administration to broiler chickens enhanced water intake but decreased feed consumption with increase in LW during the hot-dry season.

Ergonomic study of donkeys administered with Pycnogenol® and subjected to packing during the hot-dry season in Northern Nigeria.

The aim of the study was to determine some ergonomic parameters of donkeys administered with Pycnogenol® (PYC) and subjected to packing during the hot-dry season in northern Nigeria. Fifteen donkeys served as experimental subjects. Group 1 (n = 5) donkeys were subjected to trekking only (control), group 2 (n = 5) donkeys were subjected to packing (P-PYC), and group 3 (n = 5) were administered with PYC (10 mg/kg) and subjected to packing (P+PYC). Each group covered a distance of 10 km. Some ergonomic parameters were evaluated. The duration of work in control, P-PYC, and P+PYC donkeys were 170.0 ± 10 min (2.8 h), 126.6 ± 6.7 min (2.1 h), and 112.0 ± 3.9 min (1.9 h), respectively. The energy expenditure obtained for P-PYC group (88.3 ± 9.8 KJ/s) was significantly higher than the values of 28.3 ± 3.7 KJ/s and 73.5 ± 9.9 KJ/s obtained in control and P+PYC groups, respectively. The output was significantly higher in P+PYC group with the value of 5263.2 ± 135 m/h. In conclusion, packing the donkeys during the hot-dry season was stressful; however, PYC may have served as a potent antifatigue agent to reduce the stress.

Daily rhythms of rectal and body surface temperatures in donkeys during the cold-dry (harmattan) and hot-dry seasons in a tropical savannah.

The aim of the study was to evaluate daily rhythms of rectal temperature (RT) and body surface temperature (BST) of adult, yearling and foal donkeys during the cold-dry (harmattan) and hot-dry seasons in a tropical savanna. The RTs and BSTs of 30 clinically healthy, free-ranging donkeys were recorded using digital and infrared thermometers. The RT and BST of each donkey and thermal environmental parameters inside the pen were measured bi-hourly from 0600 to 0600 hours (GM + 1) on each experimental day. Application of single cosinor procedure showed that RT and BST exhibited varying levels of daily rhythmicity in both seasons, with values usually higher during the afternoon periods of the photophase of the light/dark cycle. The oscillatory pattern differed with season, with greater amplitude recorded during the cold-dry season than the hot-dry season. The acrophases of the RT and BST were mostly restricted to the photophase of the light/dark cycle in both seasons. The mean values of RT in the adult (37.10 ± 0.10 °C), yearling (37.19 ± 0.10 °C) and foal (37.45 ± 0.11 °C) donkeys were higher (F = 93.41, P < 0.0001) during the hot-dry season than the cold-dry season (35.40 ± 0.05 °C, 35.50 ± 0.04 °C and 36.20 ± 0.05 °C, respectively). In conclusion, seasonal changes influenced significantly the daily rhythmicity of RT and BST in donkeys, kept under natural light/dark cycle.

Diurnal rhythms of heart and respiratory rates in donkeys of different age groups during the cold-dry and hot-dry seasons in a tropical savannah.

The aim of this study was to determine the effect of season on diurnal rhythms of heart (HR) and respiratory rates (RespR) in the adult, yearling, and foal donkeys during the cold-dry and hot-dry seasons under natural light/dark cycle. The resting HR and RespR were recorded bihourly for 24 consecutive hours from 06:00 to 06:00 h (GMT +1) in 30, clinically healthy donkeys (10 adults,10 yearlings, and 10 foals). Dry-bulb temperature (DBT), relative humidity (RH), temperature-humidity index (THI) and wet-bulb globe temperature index (WBGT) inside the pen were recorded bihourly from 06:00 to 06:00 h. Values of DBT, THI, and WBGT obtained during the hot-dry season were significantly (P < 0.05) higher than corresponding values recorded during the cold-dry season. Application of single-cosinor procedure showed that HR and RespR exhibited daily rhythmicity in both seasons. The mesors of the HR in adult (41.51 ± 0.34 beats/min [bpm]), yearling (40.80 ± 0.43 bpm), and foal (47.55 ± 0.40 bpm) donkeys during the cold-dry season were significantly (P < 0.01) lower, compared to the corresponding values of 48.4 ± 0.40 bpm, 50.42 ± 0.52 bpm and 58.10 ± 0.50 bpm, respectively during the hot-dry season. The mesors of RespR in adult, yearling, and foal donkeys during the hot-dry season were higher (P < 0.05), when compared to the corresponding values recorded in the cold-dry season. The HR and RespR of foals were significantly (P < 0.05) higher than those of the adult and yearling donkeys. Amplitudes of HR and RespR were higher during the hot-dry season than the cold-dry season. In conclusion, seasonal changes affect diurnal rhythmicity of HR and RespR of adult, yearling, and foal donkeys during the cold-dry and hot-dry seasons. The HR and RespR of donkeys vary with age, with higher values in the foals than the adult and yearling donkeys in both seasons.

Daily rhythms of cloacal temperature in broiler chickens of different age groups administered with zinc gluconate and probiotic during the hot-dry season.

The aim of the experiment was to evaluate effects of zinc gluconate (ZnGlu) and probiotic administration on the daily rhythm of cloacal temperature (tcloacal) in broiler chickens of different age groups during the hot-dry season. One-day-old broiler chicks (n = 60) were divided into groups I-IV of 15 chicks per group, and treated for 35 days: Group I (control) was given deionized water; Group II, ZnGlu (50 mg/kg); Group III, probiotic (4.125 × 106 cfu/100 mL), and Group IV, ZnGlu (50 mg/kg) + probiotic (4.125 × 106 cfu/100 mL). Air dry-bulb temperature (tdb), relative humidity (RH), and temperature-humidity index (THI) inside the pen, and tcloacal of each broiler chick were obtained bihourly over a 24-h period; on days 21, 28, and 35 of the study. Values of tdb (32.10 ± 0.49°C), RH (49.94 ± 1.91%), and THI (38.85 ± 0.42) obtained were outside the thermoneutral zone for broiler chickens, and suggested that the birds were subjected to heat stress. Application of the periodic model showed disruption of daily rhythm of tcloacal in broilers on day 21, which was synchronized by probiotic administration. The administration of probiotics or ZnGlu + probiotics to a greater extent decreased the mesor and amplitude, delayed the acrophases of tcloacal in broilers, especially at day 35, as compared to the controls. Overall, the tcloacal values in broiler chickens administered with probiotic alone (41.25 ± 0.05°C) and ZnGlu + probiotic (41.52 ± 0.05°C) were lower (P < 0.001) than that of the controls (41.94 ± 0.06°C). In conclusion, probiotic alone synchronized tcloacal of the birds at day 21, and, in addition, decreased tcloacal response most, followed by its coadministration with ZnGlu, the antioxidants may be beneficial in modulating daily rhythmicity of tcloacal and alleviating adverse effects of heat stress on broiler chickens during the hot-dry season.

Effect of age, sex, physical activity and meteorological factors on haematological parameters of donkeys (Equus asinus).

The review examines the role of blood in homeostasis, diagnosis and treatment of disease as influenced by meteorological stress factors, age, sex and physical activity of the donkeys. Haematological parameters play a crucial role in clinical diagnosis of infectious and parasitic diseases, in assessing the responses of donkeys to treatment and in prevention of diseases. The changes in blood values are important in evaluating the responses of the animals to various physiologic conditions. In conclusion, haematological values of donkeys are largely influenced by age, sex, physical factors of the environment and physical activity, and consideration of the factors will aid accurate diagnosis and therapeutic evaluation of equine diseases.

Effects of age and season on haematological parameters of donkeys during the rainy and cold-dry seasons.

The aim of the study was to investigate the effects of age and season on haematological parameters of donkeys at rest during the rainy and cold-dry seasons. Thirty healthy donkeys divided into three groups based on their age served as the subjects. During each season, blood sample was collected from each donkey thrice, 2 weeks apart, for haematological analysis, and the dry-bulb temperature (DBT), relative humidity (RH) and temperature-humidity index (THI) were obtained thrice each day during the experimental period using standard procedures. During the rainy season, the mean DBT (33.05 ± 0.49 °C), RH (73.63 ± 1.09 %) and THI (84.39 ± 0.71) were higher (P < 0.0001) than the corresponding values of 24.00 ± 0.44 °C, 36.80 ± 0.92 % and 64.80 ± 0.62, during the cold-dry season. Packed cell volume (PCV), erythrocyte count [red blood cell (RBC)], haemoglobin concentration (Hb), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), platelet count (PLT), leucocyte count [white blood cell (WBC)], lymphocyte count (LYM) and neutrophil/lymphocyte ratio (N/L) were higher (P < 0.05) in adults than foals during the rainy season. The MCV, MCH, WBC, NEU, LYM and PLT of adult and yearling donkeys were higher (P < 0.05) during the rainy than the cold-dry season. The PCV, RBC, Hb, MCV, MCH, and NEU of foals were higher in the rainy than the cold-dry season. The N/L of adult and foal donkeys were higher (P < 0.05) in the rainy than in the cold-dry season. In conclusion, PCV, RBC, Hb and LYM were considerably higher in foals than yearlings or adults during the rainy season, while erythrocytic indices and platelet counts were higher in adults or yearlings than in foals in both seasons. Erythrocytic indices, PLT and N/L were higher in the rainy than the cold-dry season in adults, yearlings and foals.

Influence of season on daytime behavioral activities of donkeys in the Northern Guinea Savanna zone of Nigeria.

The present experiment was performed with the aim of investigating the effect of season on behavioral activities of donkeys during the rainy and harmattan seasons in the Northern Guinea zone of Nigeria. Sixteen apparently healthy donkeys were used as subjects and divided into four groups based on age. During each season, behavioral activities of each donkey were evaluated for three weeks using the focal animal sampling technique. The dry-bulb temperature (DBT), relative humidity (RH), and temperature-humidity index (THI) were obtained three times each day during the experimental period using standard procedures. In the rainy season, the mean DBT (31.65 ± 0.49°C), RH (73.63 ± 1.09%), and THI (84.39 ± 0.71) were significantly (P<0.0001) higher than the corresponding values of 24.00 ± 0.44°C, 36.80 ± 0.92%, and 64.80 ± 0.62 in the harmattan season. During the rainy season, the donkeys spent 60.00 ± 0.77%, 25.40 ± 0.69%, and 2.94 ± 0.21% on grazing, resting, and grooming, respectively. During the harmattan season, the donkeys spent the most time on grazing (76.76 ± 0.43%), less time on resting (11.97 ± 0.38%), and the least time on grooming (0.89 ± 0.05%). In conclusion, season and seasonal variations affect the daytime behavioral activities of donkeys in the zone, and this should be considered in husbandry practices for donkeys.