RESUMO
Toll-like receptors (TLRs) are recognized as fundamental contributors to the immune system function against infections. Hepatitis C virus (HCV) infection represents a global health problem especially in Egypt having the highest HCV prevalence worldwide where HCV infection is a continuing epidemic. The aim of the present study was to investigate the possible association between genetic variation in TLR-3 and TLR-9 and HCV infection and hepatic fibrosis in chronic HCV-positive Egyptian patients. The present study included 100 naïve chronic HCV-positive patients and 100 age- and sex-matched healthy controls. Genotyping of TLR-3 (_7 C/A [rs3775296]), TLR-3 (c.1377C/T [rs3775290]) and TLR-9 (1237T/C [rs5743836]) were done by polymerase chain reaction restriction fragment length polymorphism technique. Frequency of polymorphic genotypes in TLR-3 (_7 C/A), TLR-3 (c.1377C/T) and TLR-9 (1237T/C) were not significantly different between studied HCV-positive patients and controls with P values 0.121, 0.112, and 0.683, respectively. TLR-3 c.1377 T-allele was associated with advanced stage of hepatic fibrosis (P = 0.003).
Assuntos
Hepatite C/epidemiologia , Cirrose Hepática/epidemiologia , Polimorfismo de Nucleotídeo Único , Receptor 3 Toll-Like/genética , Receptor Toll-Like 9/genética , Adulto , Alelos , Índice de Massa Corporal , Estudos de Casos e Controles , Egito , Feminino , Predisposição Genética para Doença , Técnicas de Genotipagem , Hepacivirus/isolamento & purificação , Hepatite C/genética , Humanos , Cirrose Hepática/genética , Masculino , Pessoa de Meia-Idade , PrevalênciaRESUMO
A polymerase chain reaction (PCR), based on insertion sequence IS6110, was developed to detect Mycobacterium tuberculosis complex organisms in the blood samples of 56 tuberculosis patients and 34 healthy controls. The early secreted antigenic target 6-KDa (ESAT-6) are used to stimulate T lymphocyte subsets from tuberculosis-infected patients and the correlation of these immune responses to the genetic factors (HLA type) which determined the host immune response is evaluated. ESAT-6 derived peptides: P1 (1.05+/-0.084), P2 (1.08+0.094), P3 (1.02+ 0.086), P5 (0.98+/-0.117) & P7 (1.26+/-0.152) were significantly higher in the infected group than in non-infected one. Besides, 33 patients and 12 controls were tested for HLA-DRB, HLA-DQB1 & HLA-DPB1. Only type HLA-DRB1*15 was significantly associated with tuberculosis infection using the Chi- square test (X(2)=0.04311). By using the relative risk, some HLA types were relatively more susceptible to be associated with tuberculosis infection. HLA-DR typing of patients showed that they covered a large spectrum of HLA-DR molecules encoded by HLA-DRB1, -DRB3, -DRB4, & -DRB5 genes. However, HLA-DQ typing showed that they covered the HLA-DQB1 molecules. HLA-DP typing of patients showed that they covered a large spectrum of HLA-DP molecules encoded by HLA-DPB 1.
