RESUMO
In cells of micobacteria of all investigated samples the following ultrastructural changes were observed: disorganization of a nucleoid and citoplasm, formation of the citoplasmic vacuoles and endocellular lipide-like inclusions, and also change of the cells form into spheroid and formeless mass, disappearance of periplasmatic space, occurrence of cells of small-size and short incompletely divided cells in the samples. They were observed more often after 72 hours of exposition of the cultures with antibacterial substances, than after 24 hours of exposition. The highest concentration of these substances being used, the ultrastructural changes were more essential. No significant difference between the nature of changes in the structure of cells studied using antibacterial substances has been found.
Assuntos
Antituberculosos/farmacologia , Compostos Heterocíclicos/farmacologia , Mycobacterium bovis/efeitos dos fármacos , Mycobacterium tuberculosis/efeitos dos fármacos , Antituberculosos/síntese química , Forma Celular/efeitos dos fármacos , Meios de Cultura , Citoplasma/efeitos dos fármacos , Citoplasma/ultraestrutura , Relação Dose-Resposta a Droga , Compostos Heterocíclicos/síntese química , Mycobacterium bovis/ultraestrutura , Mycobacterium tuberculosis/ultraestrutura , Periplasma/efeitos dos fármacos , Periplasma/ultraestrutura , Sais/química , Vacúolos/efeitos dos fármacos , Vacúolos/ultraestruturaRESUMO
Antimycobacterial activity of izoniazid, fluorenon-9, fluorenizid and its five salts (Ag, Na, K, Ca, Li) in relation to Mycobacterium tuberculosis hominis H37R(V) and M. tuberculosis bovis 8 has been studied. High antimicrobial activity in vitro has been proved in respect of the explored cultures of mycobacteria. An optimum solvent has been defined for new substances of flouoren series when making bacteriological researches.
