RESUMO
Immunoreactive follicle-stimulating hormone (IR-FSH) is detected in sections of formalin-fixed and paraffin-embedded gastric mucosal tissue of normal men, using the immunoperoxidase staining technique and specific antisera to hFSH (NIDDK, NIH). Positive staining for IR-FSH was detected in the parietal cells lining the gastric glands of the intermediate zone. The staining was intracytoplasmic and distributed throughout the cytoplasm. IR-FSH was also found to be present in the basal part of the foveolar epithelium. Stromal tissue and nuclei were devoid of the stain. The zymogen cells in the deeper region of the mucosa did not show any detectable staining for IR-FSH. The presence of IR-FSH in gastric mucosa was also detected by radioimmunoassay. Gel chromatography of the gastric tissue extract showed a single peak of FSH immunoreactivity that coeluted with the 125I-labeled highly purified FSH preparation (NIDDK, NIH). Furthermore, the FSH in the pituitary tissue extract had a chromatographic profile similar to that of IR-FSH from gastric tissue, and 125I-FSH labeled highly purified FSH, indicating a close resemblance in their molecular sizes. These results demonstrate that IR-FSH is present in the normal human gastric mucosa. The role of this regulatory petpide in gastric tissue, if any, needs to be investigated.
Assuntos
Hormônio Foliculoestimulante/metabolismo , Mucosa Gástrica/metabolismo , Cromatografia , Mucosa Gástrica/citologia , Mucosa Gástrica/ultraestrutura , Humanos , Imuno-Histoquímica/métodos , Radioimunoensaio , Valores de Referência , Coloração e Rotulagem , Distribuição TecidualRESUMO
Using indirect immuno-peroxidase staining technique, localization of immunoreactive follicle-stimulating hormone (IR-FSH) is demonstrated in the cytoplasm of the epithelial cells of normal human stomach. In view of their triangular shape and central nucleus and their predominance in the intermediate glands of the gastric mucosa, these cells are identified as parietal cells. The stromal tissue is devoid of staining reaction.
Assuntos
Hormônio Foliculoestimulante/análise , Células Parietais Gástricas/análise , Citoplasma/análise , Mucosa Gástrica/análise , Humanos , Técnicas Imunoenzimáticas , MasculinoRESUMO
Inhibin biosynthesis by human prostatic tissue was investigated in vitro. Serum levels of inhibin as well as tissue concentrations in different cells and zones of the normal and benign hyperplastic prostates were determined. Immunocytochemical localization of inhibin identified the involvement of epithelial but not stromal cells in the synthesis and release of prostatic inhibin into the circulation. The endocrine and paracrine functions of prostatic inhibin remain to be elucidated.
