[Antibiotics formed by Actinomadura fulvescens]. / Antibiotiki, obrazuemye Actinomadura fulvescens.

Novel antibiotics with in vitro activity against gram-positive bacteria were isolated from Actinomadura fulvescens INA 3321 and INA 3852. Conditions for biosynthesis and isolation of antibiotics 3321 and 3852, as well as their physicochemical and biological properties were studied. Chromatographic analysis of the antibiotics revealed that each of them contained two biologically active components. The components were separated with preparative chromatography. Physicochemical properties of the components showed that antibiotics 3321 and 3852 were similar. UV and IR spectroscopy suggested that antibiotics 3321 and 3852 were original compounds not described earlier.

[Determination of carminomycin in its preparations by high-performance liquid chromatography]. / Opredelenie karminomitsina v ego preparatakh metodom vysokoéffektivnoi zhidkostnoi khromatografii.

A three component system for separating a mixture of carminomycin, carminomycinone and 13-dihydrocarminomycinone by HPLC was developed. Spherisorb ODS Column, 4.6 X 250 mm, the particle size of 10 micron was used. The impact of the mobile phase composition, temperature during chromatography and buffer solution pH on the capacity factors K' for every of the above compounds was studied. For determining purity of carminomycin dosage forms the procedure with an external standard was applied. The procedure provides routine quantitative assay of carminomycin hydrochloride dosage forms.

[Quantitative determination of the antibiotic doxorubicin by high-performance liquid chromatography]. / Kolichestvennoe opredelenie antibiotika doksorubitsina metodom vysokoéffektivnoi zhidkostnoi khromatografii.

A new three-component system was developed for separation of a mixture containing 6 components: doxorubicin, rubomycin, dihydrorubomycin, 14-bromrubomycin, doxorubicinone and rubomycinone. The system uses reverse phase high performance liquid chromatography. Separation was performed on a column with Spherisorb C18 (4.6 X 250 mm) with the particle size of 10 microns. Relationships between the capacity factor k1 for the above 6 components and relative arresting alpha for 3 hardly separating components: doxorubicin, rubomycinone and dihydrorubomycin, as well as between the percentage content of the organic component, acetonitrile and the percentage content of the buffer solution were studied. The external standard method was used for determination of purity of doxorubicin standard preparations. The method provides routine quantitative assay of doxorubicin hydrochloride.

[Structure of carminomycins II and III]. / Struktura karminomitsinov II i III.

Carminomycins II and III, the main components of the carminomycin complex were isolated in pure state. Their crystalline exalates and acetate of cardminomycin II were prepared. The PMR spectra of both carminomycins and the 13C-NMR spectra of the oxalates were obtained. The molecular weights of the antibiotics were determined by mass-spectrometry. On the basis of the PMR spectra it was shown that carminomycins II and III had similar structures and differed in the stereoisomerism of the nitrogen-free fragment linked to the amino sugar. This was confirmed by the 13C-NMR spectra. The above fragment (C7H15O3) is analogous to the fragment of baumycins A1 and A2 described earlier.

[Minor components from the carminomycin complex]. / Minornye komponenty iz karminomitsinovogo kompleksa.

Three components differing by their properties from the carminomycins described earlier were isolated from the carminomycin complex. Comparison of the IR and UV spectra, as well as chromatographic and physicochemical properties of 2 of them showed that they were dihydrocarminomycin and its aglycone or dihydrocarminomycinone, which was prepared earlier by synthesis. The third component was a chromophore belonging to 1,4,6-trihydroxyanthraquinone. Investigation of its IR spectrum, physicochemica properties and PMR spectrum showed it to be carboxymethylethylcarminomycinone identical to epsilon-rodomycinone. The data were confirmed by 13C-NMR spectrometry.